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276 results for “spermatogenesis”
spermatogenesis across mammals - Ensembl Release 112 mapping
<p>This repo contains remapped single-cell Anndata (h5ad) objects from the original publication "The molecular evolution of spermatogenesis across mammals (Florent Murat, Noe Mbengue et al 2023; https://doi.org/10.1038/s41586-022-05547-7)". Data was (pseudo-)remapped against Ensembl Release 112 genomes and annotations with kb-python (v0.28.2). For Macaca mulatta the NCBI GCF_003339765.1_Mmul_10_genomic.fna and GCF_003339765.1_Mmul_10_genomic.gtf was used due to low protein number in Ensembl Release 112 file Macaca_mulatta.Mmul_10.pep.all.fa.gz. Only filtered counts are provided. </p> <p>If you use this data, please cite Murat and Mbengue et al. 2023.</p>
Figure 5 in Investigation of protective effects of lithium borate on spermatogenesis and testes histopathology against cadmium-induced acute toxicity in rats
Figure 5. Testes tissue, control and LTB groups, negative COX-2 expression (AC), Cd group, severe COX-2 expressions in damaged tubules and intertubular intervals (B), LTB + Cd group, mild COX-2 expression in interstitial tissue (arrowheads) (D), IHC - P, Bar: 20µm.
Figure 4 in Investigation of protective effects of lithium borate on spermatogenesis and testes histopathology against cadmium-induced acute toxicity in rats
Figure 4. Testes tissue, Control and LTB groups, negative 8-OHdG expression (AC), Cd group, severe cytoplasmic 8-OHdG expressions in spermatocytes and spermatogonium in damaged tubules (arrowheads) (B), LTB + Cd group, mild cytoplasmic 8-OHdG expression in spermatocytes (arrowheads) (D), IHC - P, Bar: 20 µm.
Figure 3. Negative Caspase-3 in Investigation of protective effects of lithium borate on spermatogenesis and testes histopathology against cadmium-induced acute toxicity in rats
Figure 3. Negative Caspase-3 expressions in testes tissues of control and LTB groups (A and C), severe Caspase-3 expression in spermatocytes of Cd group (arrowheads) (B), mild Caspase- 3 expression in spermatocytes (arrowheads) of LTB + Cd group (D), IHC - P, Bar: 20 µm.
Figure 1 in Investigation of protective effects of lithium borate on spermatogenesis and testes histopathology against cadmium-induced acute toxicity in rats
Figure 1. Testicular tissue, normal anatomical appearance (A), oedema, hyperaemia, congestion and haemorrhage (B), normal anatomical appearance (C), moderate oedematous and mild hyperaemic (D).
Life stage-specific effects of heat stress on spermatogenesis and oogenesis in Drosophila melanogaster
<p><br>#Life stage-specific effects of heat stress on spermatogenesis and oogenesis in Drosophila melanogaster</p> <p>##Authors</p> <p>Abhishek Meena, Alessio N. De Nardo, Komal Maggu, Sonja Sbilordo, Benjamin Eggs, Rawaa Al Toma Sho, Stefan Lüpold</p> <p><br>## Citation of associated article<br>Meena, A., Maggu, K., De Nardo, A.N., Sbilordo, S.H., Eggs, B., Al Toma Sho, R., Lüpold, S., 2024. Life stage-specific effects of heat stress on spermatogenesis and oogenesis in Drosophila melanogaster. Journal of Thermal Biology 125, 104001. https://doi.org/10.1016/j.jtherbio.2024.104001</p> <p> </p> <p>##Overview<br>This dataset was collected to assess the impact of heat stress across various life stages on reproductive performance in Drosophila melanogaster. <br>The study focuses on stage- and sex-specific reproductive metrics such as: mating success, fertility, fecundity, hatching success, which for females were summed over four days and for males were assayed for four consecutive 24-hour periods, each with a different female. </p> <p>The dataset is structured to analyze these outcomes at different temperatures and developmental stages, providing insights into sex- and stage-specific vulnerabilities to heat stress. </p> <p>The analyses are performed in an R Markdown (.Rmd) file, using data stored in a CSV file</p> <p>##Contents</p> <p>The dataset includes the following files:</p> <p>Rscript.Rmd: An R Markdown file containing code for analyzing sex- and stage-specific reproductive fitness based on the data in Data.csv.<br>Data.csv: A CSV file with the raw data used for the analysis.</p> <p>##Data Description</p> <p>Note: NA's in each data file represent missing data (data not available). </p> <p>The Data.csv file contains the following columns:</p> <p>Unique.ID: Unique identifier for each experimental individual.</p> <p>SampleID: Identifier for each treatment group.</p> <p>Temperature: Thermal exposure temperature (measured in degrees Celsius) for 4 hours, with levels: 24.5°C, 28°C, 32°C, 36°C, and 38°C.</p> <p>Lifestage: Developmental stage during which thermal treatment was applied.</p> <p>Sex: Sex of the individual exposed to the thermal treatment.</p> <p>Day: Days post adult treatment exposure.</p> <p>Block: Experimental block identifier for randomized grouping.</p> <p>Total: Total number of eggs laid within 24 hours of oviposition.</p> <p>Hatched: Number of eggs that successfully hatched.</p> <p>Unhatched: Number of eggs that did not hatch (derived from the difference between Total and Hatched).</p> <p><br>##Usage</p> <p>To analyze the dataset, open and run the respective R Markdown (Rmd) files in RStudio or any compatible R Markdown environment. The Rmd files contain all the necessary code to reproduce the analyses described in the overview.</p> <p> </p>
Biogenesis of C. elegans spermatogenesis small RNAs is initiated by a zc3h12a like ribonuclease
<p><span>Small RNAs regulate spermatogenesis in many species, ranging from <em>Caenorhabditis elegans</em> to mammals. In <em>C. elegans</em>, two Argonaute proteins, ALG-3 and ALG-4, and their associated <em>alg-3/4</em> 26G-small RNAs are essential for spermatogenesis at 25°C and for imprinting paternal memory of germline gene expression in offspring. The <em>alg-3/4</em> 26G-small RNAs are antisense to their target mRNAs and are known to be produced by </span><span>the</span><span> RNA-dependent RNA polymerase, RRF-3. However, it remains unclear how the RNA templates for RRF-3 are generated and which cellular processes are affected by </span><em><span>alg-3/4</span></em><span> 26G-small RNAs</span><span>. Here, we demonstrate a key role for the conserved zc3h12a-ribonuclease-like NYN-domain-containing protein, NYN-3, in spermatogenesis at 25°C. Expression of NYN-3 was temporally coordinated with ALG-3, and our sequencing of both total and 2xFLAG::ALG-3-immunoprecipitated small RNAs revealed that NYN-3 is required for the biogenesis of <em>alg-3/4</em> 26G small RNAs. We further used ePAR-CLIP sequencing to identify NYN-3 binding sites on <em>alg-3/4</em>-targeted mature mRNAs; the NYN-3 binding sites were downstream of the binding sites for <em>alg-3/4</em> 26G-small RNAs. Additionally, 3'-RACE results placed the NYN-3 mRNA recognition upstream of RRF-3 binding and further revealed NYN-3 cleavage sites in <em>alg-3/4</em>-targeted mRNAs. Finally, a bioinformatics analysis was performed to parse the 26G small RNA-targeted genes into functional subclasses (e.g., signaling, chromatin defects). Collectively, these findings reveal NYN-3 as an initiator of small RNA generation that is central to the coordination of 26G small RNA-mediated gene regulation during spermatogenesis.</span></p>
Figures 1–4 in Testisacs microanatomy and spermatogenesis of Helobdella simplex (Hirudinida: Glossiphoniidae)
Figures 1–4. Helobdella simplex, cross section of the testisac in stages I, II and III of spermatogenesis: (1) poliplasts in the testisac lumen; (2) Stage II; (3, 4) Stage III of spermatogenesis. (C) Cytophore, (eg) spermatogonia, (em) testicular funnel, (ep) primary spermatocytes, (es) secondary spermatocytes, (et) spermatids, (ez) spermatozoa, (fa) phagocytes, (lu) testisac lumen, (m) mesothelial cell. Scale bars: 1, 3, 4 = 50 µm, 2 = 10 µm.
Biogenesis of C. elegans spermatogenesis small RNAs is initiated by a zc3h12a like ribonuclease
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Pervasive relaxed selection on spermatogenesis genes in gorillas coincident with the evolution of polygyny
<p>Gorillas have a polygynous social system in which the highest-ranking male has almost exclusive access to females and sires most of the offspring in the troop. Such behavior results in a dramatic reduction of sperm competition, which is ultimately associated with numerous traits leading to a low efficacy of gorilla spermatogenesis. However, the molecular basis behind the remarkable erosion of the gorilla male reproductive system remains unknown. Here, we explored the genetic consequences of the polygynous social system in gorillas by testing for altered selection intensity across 13,310 orthologous protein-coding genes from 261 Eutherian mammals. We identified 578 genes with relaxed purifying selection in the gorilla lineage, compared with only 96 that were positively selected. Genes under relaxed purifying selection in gorillas have accumulated numerous deleterious amino acid substitutions; their expression is biased towards male germ cells, and are enriched in functions related to meiosis and sperm biology. We tested the function of gorilla relaxed genes previously not implicated in sperm biology using the Drosophila model system and identified 41 novel spermatogenesis genes required for normal fertility. Furthermore, by exploring exome/genome sequencing data of infertile men with severe spermatogenic impairment, we found that the human orthologs of the gorilla relaxed genes are enriched for loss-of-function variants in infertile men. These data provide compelling evidence that reduced sperm competition in gorillas is associated with relaxed purifying selection on genes related to male reproductive function. The accumulation of deleterious mutations in these genes likely provides the mechanistic basis behind the low efficacy of gorilla spermatogenesis and uncovers new candidate genes for human male infertility.</p>
Data from: Phylogenetic distribution and expression pattern analyses identified a divergent basal body assembly protein involved in land plant spermatogenesis
<pre>Data from: Phylogenetic distribution and expression pattern analyses identified a divergent basal body assembly protein involved in land plant spermatogenesis Author information Shizuka Koshimizu1, Naoki Minamino2, Tomoaki Nishiyama3, Emiko Yoro4, Mayuko Sato5, Mayumi Wakazaki5, Kiminori Toyooka5, Kazuo Ebine2,6, Keiko Sakakibara4, Takashi Ueda2,6, and Kentaro Yano1 1 School of Agriculture, Meiji University, Kawasaki 214-8571, Japan 2 Division of Cellular Dynamics, National Institute for Basic Biology, Okazaki 444-8585, Japan 3 Research Center for Experimental Modeling of Human Disease, Kanazawa University, Kanazawa 920-0934, Japan 4 Department of Life Science, Rikkyo University, Tokyo 171‐8501, Japan 5 RIKEN Center for Sustainable Resource Science, Yokohama 230-0045, Japan 6 Department of Basic Biology, SOKENDAI (The Graduate University for Advanced Studies), Okazaki 444-8585, Japan This directory contains 0_README (this file) 1_Marchantia_FL_data 2_Physcomitrella_FL_data 3_Physcomitrella_TEM_images 4_PAML 1_Marchantia_FL_data Raw image data using quantification of spermatid phenotypes in Marchantia. quantification.xlsx: Summary of quantification data. 211111 and 211210: Observation date. ∟Mpbld10-1, Mpbld10-2, and Tak-1: Observed lines. ∟raw data: *.lsm files are raw image data. ∟binary image: *.tif files are binarization images of the Hoechst33342 data. ∟DIC: *.tif files are maximum intensity projection images of the C2 channels (detection of DIC images) of the raw data. ∟Hoechst33342: *.tif files are maximum intensity projection images of the C1 channels (detection of Hoechst33342 signals) of the raw data. 2_Physcomitrella_FL_data Merged data of DIC and Hoechst33342 signal images using quantification of spermatid phenotypes in Physcomitrella. line22-*.png: The images of Ppbld10-22 mutant. line30-*.png: The images of Ppbld10-30 mutant. wt.png: The images of wild type. Number in the images 1: With flagella 2: Without flagella 3_Physcomitrella_TEM_images TEM images of spermatids in the Ppbld10-30 mutant, except for images shown in Supporting Information Fig. S11. 4_PAML Files using analysis by PALM. input.fasta: Input multi fasta file. species_tree.nwk: The gene tree file for the global clock model (rooted using chlorophytes as an outgroup). species_tree_marked.nwk: A gene tree file with marks specifying land plant stem and crown branches as category #1 (for a local clock model). clock1: A directory containing output files of a PAML run with the global clock model (clock = 1) clock2: A directory containing output files of a PAML run with a local clock model (clock = 2). The branches assumed to have a different rate (r1) than the default rate r0 are specified with #1 in species_tree_marked.nwk.</pre>
Developmental heat stress interrupts spermatogenesis inducing early male sterility in Drosophila melanogaster
<p><span>Thermal stress leads to fertility reduction, can cause temporal sterility and thus results in fitness loss with severe ecological and evolutionary consequences, e.g., threatening species persistence already at sub-lethal temperatures. For males we here tested which developmental stage is particularly sensitive to heat stress in the model species <em>Drosophila</em> <em>melanogaster</em>. As developmental stages characterize the different steps of sperm development, we could narrow down which particular processes are heat sensitive. We studied early male reproductive ability and, by following recovery dynamics after a move to benign temperatures, we investigated the mechanisms behind a subsequent gain of fertility. We found strong support to suggest that the last steps of spermatogenesis are particularly sensitive to heat stress, as processes occurring during the pupal stage were mostly interrupted, delaying both sperm production and sperm maturation. Moreover, further measurements in the testes and for proxies of sperm availability indicating the onset of adult reproductive capacity matched the expected heat-induced delay in completing spermatogenesis. We discuss these results within the context of how heat stress affects reproductive organ function and the consequences for male reproductive potential.</span></p>
Pervasive relaxed selection on spermatogenesis genes in gorillas coincident with the evolution of polygyny
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Developmental heat stress interrupts spermatogenesis inducing early male sterility in Drosophila melanogaster
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Data from: Genetic dissection of hybrid male sterility across stages of spermatogenesis
Hybrid sterility is a common form of reproductive isolation between nascent species. Although hybrid sterility is routinely documented and genetically dissected in speciation studies, its developmental basis is rarely examined, especially in generations beyond the F1. To identify phenotypic and genetic determinants of hybrid male sterility from a developmental perspective, we characterized testis histology in 312 F2 hybrids generated by intercrossing inbred strains of Mus musculus domesticus and M. m. musculus, two subspecies of house mice. Hybrids display a range of histologic abnormalities that indicate defective spermatogenesis. Among these abnormalities, we quantified decreased testis size, reductions in spermatocyte and spermatid number, increased apoptosis of meiosis I spermatocytes, and more multinucleated syncytia. Collectively, our phenotypic data point to defects in meiosis I as a primary barrier to reproduction. We identified seven quantitative trait loci (QTL) controlling five histologic traits. A region of chromosome 17 that contains Prdm9, a gene known to confer F1 hybrid male sterility, affects multinucleated syncytia and round spermatids, potentially extending the phenotypic outcomes of this incompatibility. The X chromosome also plays a key role, with loci affecting multinucleated syncytia, apoptosis of round spermatids, and round spermatid numbers. We detected an epistatic interaction between QTL on chromosomes 17 and X for multinucleated syncytia. Our results refine the developmental basis of a key reproductive barrier in a classic model system for speciation genetics.
Glycosphingolipid Inhibition and Spermatogenesis in Man: A Pilot Study (MIG 2)
ClinicalTrials.gov study NCT00194649. IPD Sharing: Not stated. Countries: 1. Publications: 8.
Effect of TPN171H on Spermatogenesis
ClinicalTrials.gov study NCT05585931. IPD Sharing: Not stated. Countries: 1. Publications: 1.
ITT-5 Mechanisms of Spermatogenesis in Man
ClinicalTrials.gov study NCT02147964. IPD Sharing: UNDECIDED. Countries: 1. Publications: 12.
Rad21l1 cohesin subunit is dispensable for spermatogenesis but not oogenesis in zebrafish
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Data from: Genetic dissection of hybrid male sterility across stages of spermatogenesis
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Allen Brain Atlas
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OpenNeuro
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