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Supplementary material 3 from: Gladstone NS, Niemiller ML, Pieper EB, Dooley KE, McKinney ML (2019) Morphometrics and phylogeography of the cave-obligate land snail Helicodiscus barri (Gastropoda, Stylommatophora, Helicodiscidae). Subterranean Biology 30: 1-32. https://doi.org/10.3897/subtbiol.30.35321

: Data type: multimedia

Supplementary material 2 from: Gladstone NS, Niemiller ML, Pieper EB, Dooley KE, McKinney ML (2019) Morphometrics and phylogeography of the cave-obligate land snail Helicodiscus barri (Gastropoda, Stylommatophora, Helicodiscidae). Subterranean Biology 30: 1-32. https://doi.org/10.3897/subtbiol.30.35321

: Data type: multimedia

Supplementary material 1 from: Gladstone NS, Niemiller ML, Pieper EB, Dooley KE, McKinney ML (2019) Morphometrics and phylogeography of the cave-obligate land snail Helicodiscus barri (Gastropoda, Stylommatophora, Helicodiscidae). Subterranean Biology 30: 1-32. https://doi.org/10.3897/subtbiol.30.35321

: Data type: list

Supplementary material 4 from: Gladstone NS, Niemiller ML, Pieper EB, Dooley KE, McKinney ML (2019) Morphometrics and phylogeography of the cave-obligate land snail Helicodiscus barri (Gastropoda, Stylommatophora, Helicodiscidae). Subterranean Biology 30: 1-32. https://doi.org/10.3897/subtbiol.30.35321

: Data type: multimedia

Figure 7 from: Gladstone NS, Niemiller ML, Pieper EB, Dooley KE, McKinney ML (2019) Morphometrics and phylogeography of the cave-obligate land snail Helicodiscus barri (Gastropoda, Stylommatophora, Helicodiscidae). Subterranean Biology 30: 1-32. https://doi.org/10.3897/subtbiol.30.35321

Figure 7 Geographic distribution of MOTUs generated from the mPTP delimitation method in relation to karst adapted from Weary and Doctor (2014). Triangles represent cave populations. The numbers associated with each unique color corresponds to the associated mPTP MOTUs found in Table 3.

Figure 6 from: Gladstone NS, Niemiller ML, Pieper EB, Dooley KE, McKinney ML (2019) Morphometrics and phylogeography of the cave-obligate land snail Helicodiscus barri (Gastropoda, Stylommatophora, Helicodiscidae). Subterranean Biology 30: 1-32. https://doi.org/10.3897/subtbiol.30.35321

Figure 6 PCA results from both geometric morphometric (left) and traditional morphometric (right) analyses. A, B Total morphometric dataset (n=65) grouped by physiographic province. C, D Morphometric dataset with complimentary molecular data (n=39) grouped by MOTUs from the mPTP analysis.

Figure 3 from: Gladstone NS, Niemiller ML, Pieper EB, Dooley KE, McKinney ML (2019) Morphometrics and phylogeography of the cave-obligate land snail Helicodiscus barri (Gastropoda, Stylommatophora, Helicodiscidae). Subterranean Biology 30: 1-32. https://doi.org/10.3897/subtbiol.30.35321

Figure 3 Haplotype network generated using the NeighborNet network method with uncorrected p-distances with the CO1 dataset. Species delimitation results are depicted using major color groups for the mPTP results, and subcolor groups for the ABGD results.

Figure 5 from: Gladstone NS, Niemiller ML, Pieper EB, Dooley KE, McKinney ML (2019) Morphometrics and phylogeography of the cave-obligate land snail Helicodiscus barri (Gastropoda, Stylommatophora, Helicodiscidae). Subterranean Biology 30: 1-32. https://doi.org/10.3897/subtbiol.30.35321

Figure 5 Phylogenetic trees of the concatenated mtDNA (CO1 + 16S; 1316 bp) and the full mtDNA + nDNA (CO1 + 16S + 28S + H3; 3040 bp) datasets. Posterior probabilities generated from the analyses are shown for each clade with the top numbers. Confidence values given from the bootstrapped ML method are shown for each clade with the bottom numbers. The 'x' symbols indicate varying topology between the BI and ML analyses. ML trees are reported in the Appendix for cross-reference. Species delimitation results are depicted using major color groups for the mPTP results, and subcolor groups for the ABGD results.

Figure 2 from: Gladstone NS, Niemiller ML, Pieper EB, Dooley KE, McKinney ML (2019) Morphometrics and phylogeography of the cave-obligate land snail Helicodiscus barri (Gastropoda, Stylommatophora, Helicodiscidae). Subterranean Biology 30: 1-32. https://doi.org/10.3897/subtbiol.30.35321

Figure 2 A Landmark scheme for geomorphometric analyses. Red circles represented landmarks (LM), blue circles represent semi-landmarks (SLM). B Shell measurements utilized for the traditional morphometric (TM) analyses.

Figure 1 from: Gladstone NS, Niemiller ML, Pieper EB, Dooley KE, McKinney ML (2019) Morphometrics and phylogeography of the cave-obligate land snail Helicodiscus barri (Gastropoda, Stylommatophora, Helicodiscidae). Subterranean Biology 30: 1-32. https://doi.org/10.3897/subtbiol.30.35321

Figure 1 Geographic distribution of Helicodiscusbarri from this study in relation to karst adapted from Weary and Doctor (2014). Triangles represent cave populations.

Figure 4 from: Gladstone NS, Niemiller ML, Pieper EB, Dooley KE, McKinney ML (2019) Morphometrics and phylogeography of the cave-obligate land snail Helicodiscus barri (Gastropoda, Stylommatophora, Helicodiscidae). Subterranean Biology 30: 1-32. https://doi.org/10.3897/subtbiol.30.35321

Figure 4 Phylogenetic tree of the CO1 dataset (808 bp) using the BI methodology. Posterior probabilities generated from the analysis are shown for each clade with the top numbers. Confidence values given from the bootstrapped ML method are shown for each clade with the bottom numbers. The 'x' symbols indicate varying topology between the BI and ML analyses. ML trees are reported in the Appendix for cross-reference. Species delimitation results are depicted using major color groups for the mPTP results, and subcolor groups for the ABGD results.