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Microbial Observatory at North Temperate Lakes LTER High-resolution temporal and spatial dynamics of microbial community structure in freshwater bog lakes 2005 - 2009 original format (Reformatted to the ecocomDP Design Pattern)
This data package is formatted as an ecocomDP (Ecological Community Data Pattern). For more information on ecocomDP see https://github.com/EDIorg/ecocomDP. This Level 1 data package was derived from the Level 0 data package found here: https://pasta.lternet.edu/package/metadata/eml/knb-lter-ntl/349/4. The abstract below was extracted from the Level 0 data package and is included for context: The North Temperate Lakes - Microbial Observatory seeks to study freshwater microbes over long time scales (10+ years). Observing microbial communities over multiple years using DNA sequencing allows in-depth assessment of diversity, variability, gene content, and seasonal/annual drivers of community composition. Combining information obtained from DNA sequencing with additional experiments, such as investigating the biochemical properties of specific compounds, gene expression, or nutrient concentrations, provides insight into the functions of microbial taxa. Our 16S rRNA gene amplicon datasets were collected from bog lakes in Vilas County, WI, and from Lake Mendota in Madison, WI. Ribosomal RNA gene amplicon sequencing of freshwater environmental DNA was performed on samples from Crystal Bog, North Sparkling Bog, West Sparkling Bog, Trout Bog, South Sparkling Bog, Hell’s Kitchen, and Mary Lake. These microbial time series are valuable both for microbial ecologists seeking to understand the properties of microbial communities and for ecologists seeking to better understand how microbes contribute to ecosystem functioning in freshwater.
Cascade project at North Temperate Lake LTER – Daily Respiration Data for Whole Lake Nutrient Additions 2013-2015
Daily estimates of ecosystem respiration and values of covariates from surface waters of Paul, Peter, and Tuesday lakes from mid-May to early September for the years 2013, 2014, and 2015. Inorganic nitrogen and phosphorus were added to Peter and Tuesday lakes each year while Paul Lake was an unfertilized reference.
Moose Foraging in Temperate Forests of Central Massachusetts 2005
The "re-wilding" of ecosystems with extirpated large mammals has become a focus of recent scientific and conservation initiatives; however, it is unclear how proposed re-introductions will influence systems that are often vastly different from those that occurred before these animals were extirpated. Moose, the northeast’s largest Holocene browser, have recently expanded across southern New England’s temperate forest landscape after an absence of 200 years, realizing a natural re-wilding experiment. Moose have been well-studied throughout the boreal forest biome; however, because they are rare today in temperate forests, almost nothing is known of their ecology, behavior, or potential impacts to these ecosystems. This study investigated patterns of winter moose browse in order to: (1) gain insight into the likely influences of this herbivore on the vegetation patterns of the region; and (2) to identify the most important habitat features influencing moose winter foraging activity at a landscape and site scale. Two large forested watersheds in Central Massachusetts were sampled for moose browse, habitat features, and disturbances including forest harvesting and human activity. Chi-square and t-tests were used to identify browse species preferences of moose, and step-wise multiple regression was used to identify habitat variables that are strong predictors of browse intensity. Hardwoods and hemlock were favored over white pine, and browse intensity was significantly and positively related to forest harvesting, elevation, swamps, and distance to human settlement. The results from this study suggest that in the winter months, moose populations are concentrating in remote, elevated areas that are broken by swamps and have intensive forest harvests. In areas that support high moose densities, selective browsing, particularly in regenerating harvests, could promote less favored species like white pine at the expense of hardwoods and hemlock. The strong association between
Proteolytic Enzyme Activity in Temperate Forest Soils at Harvard Forest and Pisgah State Forest 2007-2010
The objective of this research is to investigate the processes that limit or promote the activity and production of proteolytic enzymes in temperate forest soils. To meet this objective, we performed a series of integrated observations and experiments to investigate a conceptual model of proteolytic enzyme activity whereby activity is a function of the interaction between four parameters: soil temperature and moisture, substrate concentration, and the enzyme pool size. We used four dominant temperate forest tree species that differ in SOM chemistry and the enzymatic capabilities of their fungal symbionts as a model system. These four species differed in mycorrhizal association, with white ash (Fraxinus americana) and sugar maple (Acer saccharum) supporting arbuscular mycorrhizal (AM) fungi and eastern hemlock (Tsuga canadensis) and American beech (Fagus grandifolia) supporting ectomycorrhizal (ECM) fungi. Further, the ECM associated species have leaf litter and SOM that is characterized by higher ratios of C:N than the AM associated. Soil samples were collected two sites, one located at the Prospect Hill Tract of the Harvard Forest and the other at the Pisgah State Forest. The sites have similar land use history and stand age. Soils at both sites are inceptisols classified as Typic Dystrochrepts derived from glacial till overlying granite-schist-gneiss bedrock. Experimental plots dominated by one of four target tree species were established at each site. Stands of sugar maple (Acer saccharum) and American beech (Fagus grandifolia) were located in Pisgah. Stands of Eastern hemlock (Tsuga canadensis) and white ash (Fraxinus americana) were located in Harvard Forest. At a later date, plots were also established in a red oak (Quercus rubra) stand on Prospect Hill in the MES tower footprint. At each site we located six replicate, 8 m radius, monodominant plots that were based on the following criteria: (1) more than 80% of the standing basal area was composed of the targ
Hydraulic Pathways in Leaves of Temperate Trees at Harvard Forest 2002
The transport of water, sugar and nutrients in trees is restricted to specific vascular pathways, and thus organs may be relatively isolated from one another (=sectored). Strongly sectored leaf-to-leaf pathways have been shown for the transport of sugar and signal molecules within a shoot, but not previously for water transport. The hydraulic sectoriality of leaf-to-leaf pathways was determined for current year shoots of six temperate deciduous tree species (three ring-porous: Castanea dentata, Fraxinus americana and Quercus rubra, and three diffuse-porous: Acer saccharum, Betula papyrifera and Liriodendron tulipifera). Hydraulic sectoriality was determined using dye staining and a hydraulic method. In the dye method, leaf blades were removed, and dye was forced into the most proximal petiole. For each petiole we counted the vascular traces shared with the proximal petiole. For other shoots, measurements were made of the leaf-area specific hydraulic conductivity for leaf-to-leaf pathways (kLL). In five of six species patterns of sectoriality reflected phyllotaxy; both the sharing of vascular bundles between leaves and kLL were higher for orthostichous than non-orthostichous leaf pairs. Species-differences in leaf-to-leaf sectoriality were determined as the proportional differences between non-orthostichous vs. orthostichous leaf pairs in their staining of shared vascular bundles and in their kLL; for the six species these two indices of sectoriality were strongly correlated (R2 = 0.94; P less than 0.001). Species varied 8-fold in their kLL-based sectoriality, and ring-porous species were more sectored than diffuse-porous species. Differential leaf-to-leaf sectoriality has implications for species-specific coordination of leaf gas exchange and water relations within a branch, especially during fluctuations in irradiance, water and nutrient availability.
Fluxes project at North Temperate Lakes LTER: Predicting Peat Depth in a North Temperate Lake District 2008
Peat deposits contain on the order of 1/6 of the Earth's terrestrial fixed carbon (C), but uncertainty in peat depth precludes precise estimates of peat C storage. To assess peat C in the Northern Highlands Lake District (NHLD), a approximately 7000 square km region in northern Wisconsin, United States, with 20 percent peatland by area, we sampled 21 peatlands. In each peatland, peat depth (including basal organic lake sediment, where present) was measured on a grid and interpolated to calculate mean depth. Our study addressed three questions: (1) How spatially variable is peat depth? (2) To what degree can mean peat depth be predicted from other field measurements (water chemistry, water table depth, vegetation cover, slope) and/or remotely sensed spatial data? (3) How much C is stored in NHLD peatlands? Site mean peat depth ranged from 0.1 to 5.1 m. Most of the peatlands had been formed by the in-filling of small lake basins (terrestrialization), and depths up to 15 m were observed. Mean peat depth for small peat basins could be best predicted from basin edge slope at the peatland/upland interface, either measured in the field or calculated from digital elevation (DEM) data (Adj. R2 = 0.70). Upscaling using the DEM-based regression gave a regional mean peat depth of 2.1 plus or minus 0.2 m (including approximately 0.1 to 0.4 m of organic lake sediment) and 144 plus or minus 21 Tg-C in total. As DEM data are widely available, this technique has the potential to improve C storage estimates in regions with peatlands formed primarily by terrestrialization. Number of sites: 21 Sampling Frequency: once for each site
North Temperate Lakes LTER: Northern Wisconsin Lake Resident Survey 2005, 2008, 2012
The purpose of this survey was to understand what lake characteristics people value most, what activities they enjoy most, and what they expect for the future of northern Wisconsin lakes. Questions covered aspects such as the property search process individuals went through leading up to their purchase of lakeshore property in Vilas County WI, what activities the individual's household participate in on lakes in Vilas County WI, their attitude about the future of their lake, their perception of the current state of their lake, and lake qualities they would like improved on their lake. Demographics of the respondents and background information about their lake were also collected. Two types of surveying methodologies were used for this survey, one being an internet-based survey, while the other was a mail survey. Surveys were conducted in 2005 and repeated in 2008.
North Temperate Lakes LTER Morphometry and Hypsometry data for core study lakes
Morphometric data for NTL study lakes. These are area and volume at one meter depth interval. hp-factor in percent volume for each depth layer NTL core study lakes
North Temperate Lakes LTER High Frequency Water Temperature Data, Dissolved Oxygen, Chlorophyll, pH - Crystal Lake 2011 - 2014
Data from the instrumented buoy on Crystal Lake include micrometeorological parameters, relative humidity, air temperature, wind velocity, wind driection (2 m height),and water temperatures, pH, chlorophyll, and dissolved oxygen measured by a sonde that is moving through the water column. Measurements were taken before, during, and after lake mixing experiment
Lake Mendota Carbon and Greenhouse Gas Measurements at North Temperate Lakes LTER 2016
This original dataset contains carbon and greenhouse gas (GHG) data collected in Lake Mendota during the summer of 2016. Data were collected between 15 April 2016 and 14 November 2016 on both Lake Mendota and its surrounding streams—four major inflows and the primary outflow of Lake Mendota. The dataset is comprised of four linked tables, corresponding to carbon and GHG measurements on Lake Mendota (lake_weekly_carbon_ghg), weekly physico-chemical sonde casts on Lake Mendota (lake_weekly_ysi), ebullition rate estimates on Lake Mendota (lake_weekly_ebullition), and carbon and physico-chemical data from the four major inflows and primary outflow of Lake Mendota (stream_weekly_carbon_ysi). These data were used to explore the relationship between organic carbon dynamics and greenhouse gas production on a eutrophic lake. From these data, it is possible to estimate daily oxygen, methane, and carbon dioxide flux on Lake Mendota during the study time period. Additional methods and applications of this data can be found in J.A. Harts Masters Thesis, University of Wisconsin-Madison Center for Limnology, May 2017.
North Temperate Lakes LTER Estimated winter inputs of stream water and groundwater to primary study lakes 1982 - 2014
This data set integrates and summarizes daily surface and groundwater inputs to 5 primary study lakes, using model estimates from a data-driven USGS hydrologic model (Hunt et al. 2013; Hunt and Walker 2017), and ice phenology data (number of days since ice-on). The lakes are Allequash, Big Muskellunge, Crystal, Sparkling, and Trout. Powers et al. (2017) used these data to estimate upper and lower bounds for exogenous chemical inputs to the lakes during winter. For a given lake and winter year, cumulative surface water and groundwater inputs were calculated across the ice cover period. For each lake, this data set reports the mean, maximum, and minimum winter water inputs observed across years, in units of water volume, % of average lake volume, and volume per winter day. Sampling Frequency: 1 per lake, with multiple summary values reported (i.e., mean, min, max). Number of sites: 5. Hunt, R.J. et al., 2013. Simulation of Climate - Change effects on streamflow, Lake water budgets, and stream temperature using GSFLOW and SNTEMP, Trout Lake Watershed, Wisconsin. USGS Scientific Investigations Report., pp.2013-5159. Available at: https://www.researchgate.net/publication/258363719_Simulation_of_Climate... Hunt, R.J., and Walker, J.F., 2017, GSFLOW groundwater-surface water model 2016 update for the Trout Lake Watershed, Wisconsin: U.S. Geological Survey data release, https://dx.doi.org/10.5066/F7M32SZ2. Powers SM, Labou SG, Baulch HM, Hunt RJ, Lottig NR, Hampton SE, Stanley EH. In press (expected 2017). Ice duration drives winter nitrate accumulation in north temperate lakes. Limnology and Oceanography Letters.
North Temperate Lakes LTER Long-term winter chemical limnology and days since ice-on for primary study lakes 1982 - 2014
This data set integrates long-term data sets on winter nutrient chemistry with ice phenology (number of days since ice-on), focusing on the subset of measurements taken during ice cover. Parameters characterizing limnology of 5 primary lakes (Allequash, Big Muskellunge, Crystal, Sparkling, and Trout lakes, are measured at one station in the deepest part of each lake at the surface, middle, and deep (~1 meter above bottom). These parameters include nitrate-N, ammonium-N, total dissolved phosphorus, dissolved inorganic carbon, water temperature, dissolved oxygen, and pH. Water temperature and dissolved oxygen values are the zonal averages from more complete depth profiles. Sampling Frequency: every 6 weeks during ice-covered season for the northern lakes. Number of sites: 5
North Temperate Lakes LTER Bythotrephes longimanus spiny water flea population monitoring in Wisconsin and Minnesota 2009 - 2014
Three data tables are included describing population dynamics for Bythotrephes longimanus, spiny water flea, in Southern Wisconsin during invasion. General monitor took place in Lake Mendota, Lake Monona, Lake Waubesa, Lake Kegonsa, Stormy Lake, Gile Flowage, Lake Gogebic. Accompanying Bythotrephes morphological measurements from Lake Mendota monitoring efforts in 2011 and 2012. Included are individual measurements of body morphology and reproductive status for ~2,500 Bythotrephes collected from Lake Mendota in 2011 and 2012. Sediment cores from Lake Mendota were analyzed for spiny water flea evidence with age of sediment estimated.
Long-term fish size data for Wisconsin Lakes Department of Natural Resources and North Temperate Lakes LTER 1944 - 2012 (Reformatted to the ecocomDP Design Pattern)
This data package is formatted as an ecocomDP (Ecological Community Data Pattern). For more information on ecocomDP see https://github.com/EDIorg/ecocomDP. This Level 1 data package was derived from the Level 0 data package found here: https://pasta.lternet.edu/package/metadata/eml/knb-lter-ntl/357/2. The abstract below was extracted from the Level 0 data package and is included for context: This dataset describes long-term (1944-2012) variations in individual fish total lengths from Wisconsin lakes. The dataset includes information on 1.9 million individual fish, representing 19 species. Data were collected by Wisconsin Department of Natural Resource fisheries biologists as part of routine lake fisheries assessments. Individual survey methodologies varied over space and time and are described in more detail by Rypel, A. et al., 2016. Seventy-Year Retrospective on Size-Structure Changes in the Recreational Fisheries of Wisconsin. Fisheries, 41, pp.230-243. Available at: http://afs.tandfonline.com/doi/abs/10.1080/03632415.2016.1160894
Long-term fish abundance data for Wisconsin Lakes Department of Natural Resources and North Temperate Lakes LTER 1944 - 2012 (Reformatted to the ecocomDP Design Pattern)
This data package is formatted as an ecocomDP (Ecological Community Data Pattern). For more information on ecocomDP see https://github.com/EDIorg/ecocomDP. This Level 1 data package was derived from the Level 0 data package found here: https://pasta.lternet.edu/package/metadata/eml/knb-lter-ntl/356/3. The abstract below was extracted from the Level 0 data package and is included for context: This dataset describes long-term (1944-2012) variations in the relative abundance of fish populations representing nine species in Wisconsin lakes. Data were collected by Wisconsin Department of Natural Resource fisheries biologists as part of routine lake fisheries assessments. Individual survey methodologies varied over space and time and are described in more detail by Rypel, A. et al., 2016. Seventy-Year Retrospective on Size-Structure Changes in the Recreational Fisheries of Wisconsin. Fisheries, 41, pp.230-243. Available at: http://afs.tandfonline.com/doi/abs/10.1080/03632415.2016.1160894
North Temperate Lakes LTER Processed eddy covariance time series fluxes from tower located on roof of the CFL building oriented toward Lake Mendota 2012 - current
We calculated eddy covariance based fluxes of CO2, H2O, heat, and momentum to study lake-atmosphere exchanges since 2012. These data were collected by Ankur Desai from 2012 to present using a CSAT-3 sonic anemometer and LI-7500 gas analyzer located on the roof of the CFL building. A footprint model (Kljun) was used to screen for lake only data.
Cascade Project at North Temperate Lakes LTER Core Data Carbon 1984 - 2023
Data on dissolved organic and inorganic carbon, particulate organic matter, partial pressure of CO2 and absorbance at 440nm. Samples were collected with a Van Dorn sampler. Organic carbon and absorbance samples were collected from the epilimnion, metalimnion, and hypolimnion. Inorganic samples were collected at depths corresponding to 100%, 50%, 25%, 10%, 5%, and 1% of surface irradiance, as well as one sample from the hypolimnion. Samples for the partial pressure of CO2 were collected from two meters above the lake surface (air) and just below the lake surface (water). Sampling frequency: varies; number of sites: 14
Cascade Project at North Temperate Lakes LTER Core Data Nutrients 1991 - 2019
Physical and chemical variables are measured at one central station near the deepest point of each lake. In most cases these measurements are made in the morning (0800 to 0900). Vertical profiles are taken at varied depth intervals. Chemical measurements are sometimes made in a pooled mixed layer sample (PML); sometimes in the epilimnion, metalimnion, and hypolimnion; and sometimes in vertical profiles. In the latter case, depths for sampling usually correspond to the surface plus depths of 50percent, 25percent, 10percent, 5percent and 1percent of surface irradiance. The 1991-1999 chemistry data was obtained from the Lachat auto-analyzer. Like the process data, there are up to seven samples per sampling date due to Van Dorn collections across a depth interval according to percent irradiance. Voichick and LeBouton (1994) describe the autoanalyzer procedures in detail. Nutrient samples were sent to the Cary Institute of Ecosystem Studies for analysis beginning in 2000. The Kjeldahl method for measuring nitrogen is not used at IES, and so measurements reported from 2000 onwards are Total Nitrogen.
Cascade Project at North Temperate Lakes LTER Core Data Physical and Chemical Limnology 1984 - 2016
Physical and chemical variables are measured at one central station near the deepest point of each lake. In most cases these measurements are made in the morning (0800 to 0900). Vertical profiles are taken at varied depth intervals. Chemical measurements are sometimes made in a pooled mixed layer sample (PML); sometimes in the epilimnion, metalimnion, and hypolimnion; and sometimes in vertical profiles. In the latter case, depths for sampling usually correspond to the surface plus depths of 50percent, 25percent, 10percent, 5percent and 1percent of surface irradiance.
Cascade Project at North Temperate Lakes LTER Core Data Phytoplankton 1984 - 2015
Data on epilimnetic phytoplankton from 1984-2015, determined by light microscopy from pooled Van Dorn samples at 100 percent, 50 percent, and 25 percent of surface irradiance. St. Amand (1990) and Cottingham (1996) describe the counting protocols in detail. Samples after 1995 were counted by Phycotech Inc. (http://www.phycotech.com). Sampling Frequency: varies; Number of sites: 5
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Allen Brain Atlas
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Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.