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471 results for “testis”
Single Cell RNAseq of Mouse Testis
<p>This is the processed data from the publication: "Unified single-cell analysis of testis gene regulation and pathology in 5 mouse strains" (https://doi.org/10.1101/393769)</p> <p>The raw data is avaliable at GEO: GSE113293</p> <p>Associated software is at https://zenodo.org/badge/latestdoi/140632831</p> <p>SDA_objects.zip contains key tables required for many functions, download this to use the shiny app. Contents:</p> <ul> <li>cell_data: data.table containing metadata of the cells. 80 columns including cell id, SDA component cell scores, Tsne coordinates, pseudotime, experimental group etc.</li> <li>data: a sparse matrix of normalised read counts (20322 cells by 19262 genes)</li> <li>gene_annotations: data.table containing gene locations, enrichment vs other tissues from bulk data, infertility gene status</li> <li>GO_enrich: data.table containing gene ontology enrichments for each component, the genes enriched, p.values and enrichment values</li> <li>principal_curves: list of output of princurve() containing the pseudotime trajetories</li> <li>SDAresults: output of SDA run loaded using SDAtools::load_results()</li> </ul> <p>Other R objects include:</p> <ul> <li>QC_count_matrix: Sparse matrix of raw count values for QC cells and genes</li> <li>cell_imputation_AUCs: data.table of PRAC AUC values for unimputed (train), mean cell, SDA (predict), ICA, PCA, NNMF, and MAGIC</li> <li>HocomocoV11_motifProbs_matrix: Matrix of regulation probabilities from MotifFinder using fixed motifs from HocomocoV11 database</li> <li>motifFinder_denovo: list of results of MotifFinder denovo motifs from promoters regions of genes from each component</li> <li>motifFinder_denovo_fixed: list of results of MotifFinder using 125 denovo motifs with fixed motif on all genes.</li> <li>tomtom_matched_motifs: data.table of TOMTOM matches of denovo motifs, plus metadata</li> </ul>
Assembled transcriptomes of ovary, testis, and brain (male and female) of Amphibolurus muricatus (jacky dragon) generated using Trinity v2.11.0
<p><strong><em>A. muricatus</em> transcriptome assemblies generated using Trinity v2.11.0 (Haas et al. 2013; Grabherr et al. 2011; Henschel et al. 2012)</strong><br> • Amphibolurus-muricatus_brain.fa.tar.gz: Combined Trinity assembly of <em>A. muricatus</em> brain (male and female).<br> • Amphibolurus-muricatus_combined.fa.tar.gz: Combined Trinity assembly of <em>A. muricatus</em> ovary, testis, and brain (male and female).<br> • Amphibolurus-muricatus_female_brain.fa.tar.gz: Trinity assembly of female <em>A. muricatus</em> brain.<br> • Amphibolurus-muricatus_male_brain.fa.tar.gz: Trinity assembly of male <em>A. muricatus</em> brain.<br> • Amphibolurus-muricatus_ovary.fa.tar.gz: Trinity assembly of <em>A. muricatus</em> ovary.<br> • Amphibolurus-muricatus_testis.fa.tar.gz: Trinity assembly of <em>A. muricatus</em> testis.</p> <p> </p> <p><strong>References</strong></p> <ul> <li>Grabherr, M.G., B.J. Haas, M. Yassour, J.Z. Levin, D.A. Thompson et al., 2011 Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol 29 (7):644-652.</li> <li>Haas, B.J., A. Papanicolaou, M. Yassour, M. Grabherr, P.D. Blood et al., 2013 De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis. Nat Protoc 8 (8):1494-1512.</li> <li>Henschel, R., M. Lieber, L.-S. Wu, P.M. Nista, B.J. Haas et al., 2012 Trinity RNA-Seq assembler performance optimization, pp. 45 in Proceedings of the 1st Conference of the Extreme Science and Engineering Discovery Environment: Bridging from the eXtreme to the campus and beyond. Association for Computing Machinery, Chicag, IL, USA.</li> </ul> <p> </p>
The organophosphate pesticide methamidophos opens the blood-testis barrier and covalently binds to ZO-2 in mice
<p>We studied biological effects and post-translational modifications of proteins after treating mice with the pesticide methamidophos.</p> <p>This data set provides evidence for the modification of ZO-2, indicating that the blood-testis barrier in mouse was crossed.</p>
Þekil1. Cicadatra persicaÕda erkek reme organÝ Ts.: Testis Vd.: Vas deferens Yd.b.: YardÝmcÝ bez Ejb.: Bulbus ejaculatorius Ejb: Ductus ejaculatorius in An Investigation on the Morphology of Genital Organs and Oviposition Capacit of Cicadatra persica Kirkaldy, 1909 (Cicadidae, Homoptera)
Þekil1. Cicadatra persicaÕda erkek Ÿreme organÝ Ts.: Testis Vd.: Vas deferens Yd.b.: YardÝmcÝ bez Ejb.: Bulbus ejaculatorius Ejb: Ductus ejaculatorius
Figure 3. Limnocoris submontandoni testis with clomazone 162 in Ecotoxicological effects of commercial herbicides on the reproductive system of aquatic arthropod Limnocoris submontandoni (Hemiptera: Naucoridae)
Figure 3. Limnocoris submontandoni testis with clomazone 162 mg / L, (A) Apparent enlargement of the testicular interstice and degeneration of some spermatoids (SPTI), with normal concentrations of spermatozoa (SPTZ) in the outer regions, (B) spermatocytes (SPTO) with normal appearance and sperm (SPTZ) with slight alteration of spermatids (SPTI), C) profusion of sperm (SPTZ) with a high degree of compaction, indicating high rates of spermatogenesis and integrity of tubular cells (arrowhead). Dye: Toluidine Blue.
Single-cell RNA sequencing of the testis of drive and standard Teleopsis dalmanni males
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Exposure to 3, 3', 4, 4', 5-pentachlorobiphenyl (PCB 126) causes widespread DNA hypomethylation in adult zebrafish testis
<p>Exposure to environmental toxicants during preconception have been shown to affect offspring health and epigenetic mechanisms such as DNA methylation are hypothesized to be involved in adverse outcomes. However, studies addressing the effects of exposure to environmental toxicants during preconception on epigenetic changes in gametes are limited. The objective of this study is to determine the effect of preconceptional exposure to a dioxin-like PCB (PCB126) on DNA methylation and gene expression in testis. Adult zebrafish were exposed to 3 and 10 nM PCB126 for 24 hours and testis tissue was sampled at 7 days post-exposure for histology, DNA methylation and gene expression profiling. Reduced Representation Bisulfite Sequencing revealed 37 and 92 differentially methylated regions (DMRs) in response to 3 and 10nM PCB126 exposures, respectively. Among them 19 DMRs were found to be common between both PCB126 treatment groups. Gene ontology (GO) analysis of DMRs revealed that enrichment of terms such as RNA processing, iron-sulfur cluster assembly and gluconeogenesis. Gene expression profiling showed differential expression of 40 and 1621 genes in response to 3 and 10nM PCB126 exposures, respectively. GO analysis revealed differential expression of genes related to xenobiotic metabolism, oxidative stress and immune function. There is no overlap in the GO terms or individual genes between DNA methylation and RNAseq results, but functionally many of the altered pathways have been shown to cause spermatogenic defects. Our results indicate that exposure to dioxin-like PCBs during preconception could affect testicular function by altering DNA methylation patterns, with significant implications for reproductive health. </p>
Exposure to 3, 3’, 4, 4’, 5-pentachlorobiphenyl (PCB 126) causes widespread DNA hypomethylation in adult zebrafish testis
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Data and code for: Relative testis size is associated with vagina length but not sperm storage traits in Galliformes
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Data from: Sperm competition and the evolution of precopulatory weapons: testis size and amplexus position, but not arm strength, affect fertilization success in a chorusing frog
Trade-offs between pre- and postcopulatory traits influence their evolution, and male expenditure on such traits is predicted to depend on the number of competitors, the benefits from investing in weapons, and the risk and intensity of sperm competition. Males of the chorusing frog Crinia georgiana use their arms as weapons in contest competition. Previously, we showed that increased numbers of rivals elevated the risk and intensity of sperm competition due to multimale amplexus, and caused a reversal in the direction of precopulatory selection on arm girth. Here we focused on the factors affecting postcopulatory fertilization success during group spawning, using paternity data from natural choruses. Competitive fertilization success depended on the time spent amplexed and amplexus position. Relative testes size but not arm girth, contributed to fertilization success, but the effect of testes size depended on amplexus position. Our findings offer within species empirical support for recent sperm competition models that incorporate precopulatory male-male competition, and show why an understanding of the evolution of animal weapons requires a consideration of both pre- and postcopulatory episodes of sexual selection.
Sperm competition drives variation in testis size within and between breeding seasons in the gracile mouse opossum
<p>Data set on <i>Gracilinanus agilis</i> (Didelphimorphia: Didelphidae) with a reproducible script.</p><p> </p>
Porcine reproductive and respiratory syndrome virus infects the reproductive system of male piglets and impairs development of the blood–testis barrier
<p>Porcine reproductive and respiratory syndrome virus (PRRSV) causes a highly contagious disease that threatens the global swine industry. Recent studies have focused on the damage that PRRSV causes to the reproductive system of male pigs, although <span>pathological research is lacking</span>. Therefore, <span>we </span><span>examined the pathogenic mechanisms in male piglets infected with PRRSV</span>. Gross and histopathological changes indicated that PRRSV affected the entire reproductive system, as confirmed via immunohistochemical analysis. PRRSV infected Sertoli cells and spermatogonia. To test the <span>new </span>hypothesis that PRRSV infection in piglets impairs blood–testis barrier (BTB) development, we investigated the <span>pathology</span> of PRRSV damage in the BTB. PRRSV infection significantly decreased the quantity and proliferative capacity of Sertoli cells constituting the BTB. Zonula occludens-1 and β-catenin were downregulated in cell–cell junctions. Transcriptome analysis revealed that several crucial genes and signaling pathways involved in the growth and development of Leydig cells, Sertoli cells, and tight junctions in <span>the </span>testes were downregulated. <span>Apoptosis, necroptosis</span>, inflammatory, and oxidative stress-related pathways were activated, whereas hormone secretion-related pathways were inhibited. Many Sertoli cells and spermatogonia underwent apoptosis during early differentiation. Infected piglets exhibited disrupted androgen secretion, leading to significantly reduced testosterone and anti-Müllerian hormone levels. A cytokine storm occurred, notably upregulating cytokines such as tumor necrosis factor-α and interleukin-6. Markers of oxidative-stress damage (i.e., H<sub>2</sub>O<sub>2</sub>, malondialdehyde, and glutathione) <span>were upregulated</span>, whereas antioxidant-enzyme activities (i.e., superoxide dismutase, total antioxidant capacity, and catalase) <span>were downregulated</span>. Our results demonstrated that PRRSV infect<span>ed</span> multiple organs in the male reproductive system, <span>which imparied</span> growth in the BTB. </p>
Sperms Activation by Direct Electrical Shocks on Testis in Infertile Males
ClinicalTrials.gov study NCT04173052. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Caudal Block vs Retrolaminar Block on Analgesic Consumption in Undescended Testis Surgeries
ClinicalTrials.gov study NCT06725862. IPD Sharing: UNDECIDED. Countries: 1. Publications: 0.
Regulation of Stem Spermatogonia in the Mature Testis
ClinicalTrials.gov study NCT02756923. IPD Sharing: NO. Countries: 1. Publications: 2.
Trial of 1 Cycle of Adjuvant BEP Chemotherapy in High Risk, Stage 1 Non-seminomatous Germ Cell Testis Tumours
ClinicalTrials.gov study NCT01726374. IPD Sharing: Not stated. Countries: 1. Publications: 1.
CMV IGG Antibodies in Semen as a Predictor for Blood Testis Barrier Damage
ClinicalTrials.gov study NCT05302856. IPD Sharing: Not stated. Countries: 1. Publications: 2.
Contralateral Testicular Volume in Unilateral Impalpable Testis
ClinicalTrials.gov study NCT05933811. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Testis Needle Aspiration of Sperm in Men With Azoospermia
ClinicalTrials.gov study NCT02418832. IPD Sharing: NO. Countries: 1. Publications: 5.
Data from: Long-term effect of yolk carotenoid levels on testis size in a precocial bird
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Allen Brain Atlas
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
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