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626 results for “testosterone”
Data for: Effects of testosterone on gene expression are concordant between sexes but divergent across species of Sceloporus lizards
<p>Hormones mediate sexual dimorphism by regulating sex-specific patterns of gene expression, but it is unclear how much of this regulation involves sex-specific hormone levels versus sex-specific transcriptomic responses to the same hormonal signal. Moreover, transcriptomic responses to hormones can evolve, but the extent to which hormonal pleiotropy in gene regulation is conserved across closely related species is not well understood. We addressed these issues by elevating testosterone levels in juvenile females and males of three <em>Sceloporus </em>lizard species prior to sexual divergence in circulating testosterone<em>, </em>then characterizing transcriptomic responses in the liver. In each species, more genes were responsive to testosterone in males than in females, suggesting that early developmental processes prime sex-specific transcriptomic responses to testosterone later in life. However, overall transcriptomic responses to testosterone were concordant between sexes, with no genes exhibiting sex-by-treatment interactions. By contrast, hundreds of genes exhibited species-by-treatment interactions, particularly when comparing distantly related species with different patterns of sexual dimorphism, suggesting evolutionary lability in gene regulation by testosterone. Collectively, our results indicate that early organizational effects may lead to sex-specific differences in the magnitude, but not the direction, of transcriptomic responses to testosterone, and that the hormone-genome interface accrues regulatory changes over evolutionary time.</p>
Data from: Extensive, transient, and long-lasting gene regulation in a song-controlling brain area during testosterone-induced song development in adult female canaries
<p>Like other canary reproductive behaviors, song production occurs seasonally and can be triggered by gonadal hormones. Adult female canaries treated with testosterone sing first songs after four days and progressively develop towards typical canary song structure over several weeks, a behavior that females otherwise rarely or never show. We compared gene regulatory networks in the song-controlling brain area HVC after 1 hour (h), 3 h, 8 h, 3 days (d), 7d, and 14d testosterone treatment with placebo-treated control females, paralleling HVC and song development. Rapid onset (1 h or less) of extensive transcriptional changes (2,700 genes) preceded the onset of song production by four days. The highest level of differential gene expression occurred at 14 days when song structure was most elaborate, and song activity was highest. The transcriptomes changed massively several times during the two-week of song production. A total of 9,710 genes were differentially expressed, corresponding to about 60% of the known protein-coding genes of the canary genome. Most (99%) of the differentially expressed genes were regulated only at specific stages. The differentially expressed genes were associated with diverse biological functions, of which cellular level occurring early and nervous system level occurring primarily after prolonged testosterone treatment. Thus, the development of adult songs requires restructuring the entire HVC, including most HVC cell types, rather than altering only neuronal subpopulations or cellular components. Parallel regulation directly by androgen and estrogen receptors and by other hub genes such as the transcription factor SP8, which are under steroidogenic control, lead to massive transcriptomic and neural changes in the specific behavior-controlling brain areas and gradual seasonal occurrence of singing behavior.</p>
Test data and analysis script for manuscript: Uncovering the complex relationship between balding, testosterone and skin cancers in men
<p>Test data for the manuscript entitled: "<strong>Uncovering the complex relationship between balding, testosterone and skin cancers in men"</strong><br> <br> Includes: <br> --Readme.txt<br> --folder: example<br> --folder: script</p>
Lifestyle Intervention and Testosterone Replacement in Obese Seniors
ClinicalTrials.gov study NCT02367105. IPD Sharing: NO. Countries: 1. Publications: 1.
Extensive, transient, and long-lasting gene regulation in a song-controlling brain area during testosterone-induced song development in adult female canaries
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Organizational effects of testosterone on the number of mating partners and reproductive success in females of a social rodent
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Data for: Effects of testosterone on gene expression are concordant between sexes but divergent across species of Sceloporus lizards
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DNA methylation associates with sex-specific effects of experimentally increased yolk testosterone in wild nestlings
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Data from: Exogenous testosterone increases the audience effect in healthy males: evidence for the social status hypothesis
<p>Several studies have implicated testosterone in the modulation of altruistic behaviours instrumental to advancing social status. Independent studies have also shown that people tend to behave more altruistically when being watched (i.e., audience effect). To date, little is known about whether testosterone could modulate the audience effect. In the current study, we tested the effect of testosterone on altruistic behaviour using a donation task, wherein participants were asked to either accept or reject a monetary transfer to a charity organization accompanying a personal cost either in the presence or absence of an observer. We administered testosterone gel or placebo to healthy young men (n = 140) in a double-blind, placebo-controlled, mixed design. Our results showed that participants were more likely to accept the monetary transfer to the charity when being observed compared to when they completed the task alone. More importantly, this audience effect was amplified among people receiving testosterone vs. placebo. Our findings suggest that testosterone administration increases the audience effect and further buttress the social status hypothesis, according to which testosterone promotes status-seeking behaviour in a context-dependent manner.</p>
Relationships between cortisol and testosterone in female titi monkeys (Plecturocebus cupreus) [dataset]
<p>[dataset for article in review]</p> <p>Steroid hormones are critical to the regulation of sociosexual behavior. Their role in the formation of pair bonds is complicated by the relative scarcity of this social system in mammals, as well as species and taxonomic differences in endocrine systems. In the present study, we experimentally manipulated the hypothalamic-pituitary-adrenal axis in female titi monkeys (<em>Plecturocebus cupreus</em>), a neotropical monkey studied for its strong, selective pair bonds. We validated a new assay for plasma and urinary cortisol in this species, showing a strong suppression of cortisol following dexamethasone injection, and a significant but somewhat blunted response to adrenocorticotrophin hormone (ACTH) stimulation. Urinary testosterone did not change in response to dexamethasone or ACTH. Plasma and urinary cortisol were highly correlated, whereas urinary cortisol and testosterone were only correlated when extreme cortisol values were included. In this study, we laid groundwork for studying the role of glucocorticoids and androgens (and eventually, their interactions with peptides) in the behavioral endocrinology of pair bonds in female titi monkeys.</p>
Data from: Testosterone activates sexual dimorphism including male-typical carotenoid but not melanin plumage pigmentation in a female bird
In males it is frequently testosterone (T) that activates the expression of sexually selected morphological and behavioral displays, but the role of T in regulating similar traits in females is less clear. Here we combine correlational data with results from T and gonadotropin-releasing hormone (GnRH) manipulations in both sexes to assess the role of T in mediating sexually dimorphic coloration and morphology in the red-backed fairy-wren (Malurus melanocephalus). We show that (1) natural variation in female expression of ornamental traits (darkened bills and red back feathers) are positively associated with age and circulating androgen titres, (2) females have the capacity to express most male-typical traits in response to exogenous T, including carotenoid-pigmented body plumage, shorter feathers, darkened bill, and enlarged cloacal protuberance, but (3) appear constrained in production of male-typical melanin-pigmented plumage, and (4) low androgen levels during the pre-nuptial molt, probably due to low ovarian capacity for steroid production (or LH-sensitivity), prevent females from developing male-like ornamentation. Thus, females appear to retain molecular mechanism for hormonally regulated male-typical ornamentation, although these are rarely activated because of insufficient production of the hormonal signal.
Data from: Female ornamentation is associated with elevated aggression and testosterone in a tropical songbird
In males, testosterone plays a key role in ornament production and linking ornamentation with reproductive behaviors and other traits to produce an integrated phenotype. Less is known about whether females couple testosterone, ornamentation, and aggressive behaviors to achieve female-specific combinations of traits. Ornamentation in females may be the result of correlated expression with male ornamentation, or female traits could arise as the result of sex specific selection pressures. Resolving between these alternatives is necessary to understand the degree to which selection acts on female traits. The White-shouldered Fairywren (Malurus alboscapulatus) provides a useful context to address these questions because populations vary in degree of female ornamentation, a derived trait, whereas male ornamentation is constant across both populations. We found that ornamented females have higher levels of circulating testosterone and respond more aggressively to experimental territorial intrusions than do unornamented females. These findings are consistent with the idea that, among female White-shouldered Fairywrens, testosterone may mechanistically link plumage and behavioral traits to produce an integrated competitive phenotype, as has been reported for males of closely related species. In contrast, circulating testosterone in males did not differ significantly between populations. More broadly, our findings are consistent with ongoing selection on the mechanisms underlying female ornaments, likely via social selection.
Inter-annual repeatability and age-dependent changes in plasma testosterone levels in a longitudinally monitored free-living passerine bird
<p>While seasonal trends in testosterone levels are known from cross-cohort studies, data on testosterone inter-annual individual repeatability in wild birds are rare. Also, our understanding of hormonal age-dependent changes in testosterone levels is limited. We assessed plasma testosterone levels in 105 samples originating from 49 repeatedly captured free-living great tits (<i>Parus major</i>) sampled during the nesting to investigate their relative long-term repeatability and within-individual changes. Furthermore, we examined the inter-annual repeatability of condition-related traits (carotenoid- and melanin-based plumage ornamentation, ptilochronological feather growth rate, body mass, and haematological heterophil/lymphocyte ratio) and their relationships to testosterone levels. We show that testosterone levels are inter-annually repeatable in females, with a non-significant pattern in males, both in absolute values and individual ranks (indicating the maintenance of relative status in a population). In males, we found a quadratic dependence of testosterone levels on age, with a peak in midlife. In contrast, female testosterone levels showed no age-dependent trends. The inter-annual repeatability of condition<span>-</span>related traits ranged from zero to moderate and was mostly unrelated to plasma testosterone concentrations. However, males with elevated testosterone had significantly higher carotenoid-pigmented yellow plumage brightness, a trait presumably involved in mating. Showing inter-annual repeatability in testosterone levels, this research opens the way to further understanding the causes of variation in condition-related traits. Based on a longitudinal dataset, this study demonstrates that male plasma testosterone undergoes age-related changes that may regulate resource allocation. Our results thus suggest that, unlike females, male birds undergo hormonal senescence similar to mammals.</p>
Beyond sex and aggression: Testosterone rapidly matches behavioral responses to social context and tries to predict the future
<p>Although androgens are widely studied in the context of aggression, androgenic influences on prosocial behaviors have been less explored. We examined testosterone's (T) influence on prosocial and aggressive responses in a positively-valenced social context (interacting with a pairbond partner) and a negatively-valenced context (interacting with an intruder) in socially monogamous Mongolian gerbils. T increased and decreased prosocial responses in the same individuals towards a pairbond partner and an intruder, respectively, both within 30 minutes, but did not affect aggression. T also had persistent effects on prosocial behavior; males in which T initially increased prosocial responses towards a partner continued to exhibit elevated prosocial responses towards an intruder male days later until a second T injection rapidly eliminated those responses. Thus, T surges can rapidly match behavior to current social context, as well as prime animals for positive social interactions in the future. Neuroanatomically, T rapidly increased hypothalamic oxytocin, but not vasopressin, cellular responses during interactions with a partner. Together, our results indicate that T can facilitate and inhibit prosocial behaviors depending on social context, that it can influence prosocial responses across rapid and prolonged time scales, and that it affects oxytocin signaling mechanisms that could mediate its context-dependent behavioral influences.</p>
Data from: Testosterone eliminates strategic prosocial behavior in healthy males
<p>Humans are strategically more prosocial when their actions are being watched by others than when they act alone. Using a psychopharmacogenetic approach, we investigated the computational and endocrinological mechanisms of such audience-driven prosociality. 187 male participants received either a single dose of testosterone or a placebo and performed a prosocial and self-benefitting reinforcement learning task. Crucially, the task was performed either in private or when being watched. Rival theories suggest that the hormone might either diminish or strengthen audience-depended generosity. We show that exogenous testosterone fully eliminated strategic, i.e., feigned, generosity and thus decreased submission to audience expectations. We performed reinforcement-learning drift-diffusion computational modeling to elucidate which latent aspects of decision-making testosterone acted on. The modeling revealed that testosterone compared to placebo did not deteriorate reinforcement learning per se, rather, when being watched, the hormone altered the degree to which the learned information on choice value translated to action selection. This indicates that when being watched, the hormone was influencing participants towards choices that are less optimal for prosociality, which resulted in nonconforming behavior. Indeed, exploratory personal values analysis suggests that the effect of testosterone in our reinforcement learning task is underpinned by a shift from socially conforming to nonconforming decisions. Together, these results indicate that instead of deceptively increasing socially desirable behavior, testosterone - by acting on situational personal values - counteracts submission to audience expectations, despite plausible reputational costs.</p>
Activational and organizational effects of testosterone on the number of mating partners and reproductive success in males of a social rodent
<p>The timing of exposure to the steroid hormone, testosterone, produces activational and organizational effects in vertebrates. These activational and organizational effects are hypothesized to relate with the number of female mating partners and reproductive success in males. We tested this hypothesis by examining 151 wild degu (<em>Octodon degus</em>) males across a 10-year study. We quantified the association between adult serum testosterone levels (i.e., an indirect index of adult activational effects) and anogenital distance (AGD) length (i.e., a direct index of fetal organizational effects), and their interaction on the number of female mating partners and reproductive success. We found no evidence of an association between adult male serum testosterone levels and the number of female mating partners, or between adult male serum testosterone levels and reproductive success. However, male AGD was positively associated with reproductive success, but not so with the number of female mating partners. Additionally, the positive association between male AGD and male reproductive success was mediated by the number of mates. Our findings do not support major roles of activational or organizational effects of testosterone on the number of female mating partners and its consequences on male reproductive success. Instead, our results suggest that compared with individual male attributes, the female social environment plays a more important role in driving male reproductive success.</p>
Data from: Androgen-armoured amazons – reversed sex roles in coucals are associated with testosterone in females but not males
<p class="MsoNormal">In some species, sexual selection is stronger in females than males. In classically polyandrous birds, for instance, females compete for mating opportunities and males care for offspring. Sex steroids such as testosterone have been suggested to regulate the behaviours of 'role-reversed' females and males, but comparative studies did not find evidence for a role of testosterone in relation to sex roles. However, the large variability of hormone measurements across laboratories may prevent detecting subtle differences in hormone levels. To circumvent this caveat, I compared sex steroid concentrations of females and males of two closely related and cohabiting species with different mating systems, the classically polyandrous black coucal (<em>Centropus grillii</em>) and the monogamous white-browed coucal (<em>C. superciliosus</em>). Baseline and GnRH-induced testosterone concentrations were twice as high in female black coucals than female white-browed coucals, and the low pre-breeding progesterone concentrations of female black coucals were consistent with progesterone's modulatory role during agonistic interactions in this species. Baseline and GnRH-induced testosterone and progesterone concentrations did not differ between males of both species. This study provides first evidence that elevated testosterone is associated with sex-role reversed traits in females, whereas low levels of testosterone may not be necessary to facilitate sex-role reversal in males.</p>
Data for: Social environment and masculinization level interact to explain male testosterone levels in a social rodent
<p><span>In vertebrates, male testosterone levels vary across the year and are generally higher during the mating period relative to the offspring-rearing period. However, male testosterone levels may also be influenced by male morphotype (males with different levels of masculinization), and by the social environment experienced within a conspecific group. In social species, it has been proposed that high levels of testosterone could be incompatible with the development of an amicable social environment. Thus, in these species, it is predicted that males have relatively low levels of testosterone. Our goal was to examine the potential association between male serum testosterone levels, male morphotype, and the social environment in the caviomorph rodent <em>Octodon</em> <em>degus</em> under natural conditions. We quantified serum testosterone levels of male degus during the periods of mating and offspring rearing, and the size and composition of social groups, including masculinization levels of male and female group members from 2009 to 2019. Our results revealed that male testosterone levels covary with the period, male morphotype, and female and male social group environments, but not with offspring social environment (total number of offspring in the social group). Additionally, male degus exhibit low levels of testosterone that are indistinguishable from female levels during mating and offspring rearing. Similar to other highly social mammals, male amicable behavior could be the best male mating strategy, implying a downregulation of males' testosterone levels.</span></p>
Male social environment and female masculinization level interact to explain female testosterone levels in a communally rearing rodent
<p><span>Testosterone is known to modulate several morphological and behavioral phenotypic traits in males. However, females also synthetize testosterone and other androgens which influence the development and function of female tissues. In vertebrates, mating and social interactions can in turn modulate individual female testosterone levels, generally being higher in females that compete for males and/or that monopolize reproduction. Female testosterone levels may also be higher in females with masculinized phenotypes or when females are exposed to the energetic demands of reproduction. We used data from a long-term study (2009–2019) in a natural population of the communally rearing rodent <em>Octodon degus</em> to examine the potential associations between female serum testosterone levels and season, female masculinization morphotype, and the individual composition within social groups. We quantified the size and composition of social groups, including the masculinization morphotype of adult male and female group members. We found that female testosterone levels were higher in masculinized vs. feminized females and were also higher during the offspring-rearing season than the mating season. Female testosterone levels also varied with the number and morphotype of male group members, but not with female or offspring social environments. Together, our results suggest that female testosterone levels are more sensitive to intersexual than to intrasexual interactions. Our results also reveal that female and male testosterone levels do not significantly differ between the sexes, a notable finding previously reported only in rock hyraxes. We discuss how the complex social system of degus could be driving this physiological similarity between the sexes.</span></p>
ORAL T-8 Oral Testosterone for Male Hormonal Contraception
ClinicalTrials.gov study NCT01167829. IPD Sharing: Not stated. Countries: 1. Publications: 16.
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