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421 results for “thrips”
Effect of sticky rice germ oil droplet spraying on chrysanthemum thrips resistance and metabolome
<p>This dataset contains experimental results from full plant assays with Chrysamthemum plants that were conducted to test the effectiveness of sprayng solutions containing sticky rice oil droplets for trapping of small arthropods on plants. The experiments were conducted at the Institute of Biology Leiden, Leiden University the Netherlands.</p> <p>The first dataset contains the results of the full plant assays with thrips.</p> <p>The second dataset contains the results of 1H NMR and GC-MS signals of leaf samples of sprayed chrysanthemum plants.</p> <p> </p> <p>Version history:</p> <p>Version 2: Included the RAW data on % coverage of plants for the two plant assays that had been left out during earlier submission</p> <p>Updated the metadatasheets within the excel files to be more complete.</p> <p>Version 3: Included a new excel sheet in the GC-MS and NMR data file in which a subset of the RAW HS-GC-MS and 1H NMR data, namely those peaks and delta signals that were identified and matchedd to compound id after untargeted analysis, are presented together with the name of the compounds or classes of compounds as mentioned in the manuscript.</p> <p>No changes were made to the plant assay data file</p> <p> </p> <p>In the "Dataset_TBierman_RGO_thrips_1HNMR_GC-MS_V3" excel file:</p> <p>Sheets: "Processed 1H NMR data" and "Processed HS-GC-MS data"</p> <p>contain processed 1H NMR and GC-MS data of chrysanthemum leaves, harvested after 10 or 25 days, of plants that were sprayed with water or vegetable-oil derived adhesives and infested with thrips or not.</p> <p>Sheet: "Quantitative data selected comp" contains a subset of the data where signals were found significant in the untargeted analysis have been annotated to their compound identity.</p> <p>In the "Dataset_TBierman_RGO_thrips_plantassay1_and_2_V3" excel file:</p> <p>Sheets "Plant_assay_1_RGO_thrips_d10_25" and "Plant_assay_2_RGO_thrips_d25" contain the raw plant assay data</p> <p>Sheets "Plant_assay_1_RGO_coverage" and "Plant_assay_2_RGO_coverage" contain the summary values of the estimated coverage with adhesive oil droplets of each respective experiment on the left side while on the right side the raw data is presented </p> <p> </p>
Development of thrips barcode database and multiplex real-time PCR assay for quarantine and agriculture pest species
<p>Thrips (Order Thysanoptera) species are agriculturally important as plant sap sucking pests and vectors of several plant diseases. They are very small insects and commonly associated with imported commodities at New Zealand border in all life stages. Morphological identification of thrips is mainly performed on adults, but the available identification keys for immature stages do not include many species and are inadequate, thus DNA barcode was regularly used for thrips identification, here, we have generated DNA barcode data for over 29 thrips species from over 100 individuals. At New Zealand border,<em> Frankliniella occidentalis </em>is the dominant species intercepted, followed by <em>F. panamensis</em>, <em>Thrips palmi</em> and <em>T. tabaci </em>and several other thrips species. Hence, we have also developed a multiplex real time PCR assay, targeting the four thrips species to facilitate the identification of quarantine interceptions with more accurate and faster diagnostic method for any developmental stages. The DNA barcode database further assists in thrip identification. The assay showed high specificity for all the four target species and could detect 10 copies/ µL of the target DNA. Linear responses and high correlation coefficients between the amount of DNA and <em>C</em><sub>q</sub> values for each species were also achieved. The method was tested on single egg, larva and adult and proved to be applicable for all life stages of the four species. This study has demonstrated the assay is a useful biosecurity tool for rapid and reliable identification of the target thrips species. </p>
Figure 55. A–C in The identification of the flower thrips, Frankliniella Karny (Thysanoptera: Thripidae) intercepted at U.S. ports of entry
Figure 55. A–C) Frankliniella williamsi. A) Body. B) Antennal segments VII–VIII. C) Abdominal tergite VIII posteromar-
Figure 49. A–E in The identification of the flower thrips, Frankliniella Karny (Thysanoptera: Thripidae) intercepted at U.S. ports of entry
Figure 49. A–E) Frankliniella schultzei A) Dark form. B) Pale form. C) Antennal segment VI subapical sense cones and basal portion of segment VII. The double headed arrows indicate areas of measure. D) Head of the dark form. E) Head of the pale form.
Figure 52. A–D in The identification of the flower thrips, Frankliniella Karny (Thysanoptera: Thripidae) intercepted at U.S. ports of entry
Figure 52. A–D) Frankliniella tritici. A) Body. B) Pedicel of antennal segment III. C) Head and pronotum. D) Abdominal tergite VIII posteromarginal comb.
Figure 50. A–E in The identification of the flower thrips, Frankliniella Karny (Thysanoptera: Thripidae) intercepted at U.S. ports of entry
Figure 50. A–E. Frankliniella suramericana, holotype, USNM 01207739. A) Body. B) Head and pronotum. C) Upper surface of a hind coxa. D) Abdominal sternite II. E) Abdominal tergite VIII posteromarginal comb.
Figure 30. A–C in The identification of the flower thrips, Frankliniella Karny (Thysanoptera: Thripidae) intercepted at U.S. ports of entry
Figure 30. A–C) Frankliniella funderburki, holotype, USNM 01207579. A) Body. B) Antennal segments I and II, head and pronotum. C) Antennal segment III basal collar and pedicel.
Figure 34. A–D in The identification of the flower thrips, Frankliniella Karny (Thysanoptera: Thripidae) intercepted at U.S. ports of entry
Figure 34. A–D) Frankliniella gossypiana A) Body. B) Head and pronotum. C) Hind coxae, abdominal sternites I and II. D) Abdominal tergite VIII posteromarginal comb.
Figure 45. A–D in The identification of the flower thrips, Frankliniella Karny (Thysanoptera: Thripidae) intercepted at U.S. ports of entry
Figure 45. A–D) Frankliniella parvula A) Body. B) Pedicel of antennal segment III. C) Ventral compound eyes. D) Head and pronotum.
Figure 29. A–D in The identification of the flower thrips, Frankliniella Karny (Thysanoptera: Thripidae) intercepted at U.S. ports of entry
Figure 29. A–D) Frankliniella fulvipennis. A) Body. B) Ventral eye. C) Head and pronotum. D) Abdominal tergite VIII posteromarginal comb.
Figure 26. A–D in The identification of the flower thrips, Frankliniella Karny (Thysanoptera: Thripidae) intercepted at U.S. ports of entry
Figure 26. A–D) Frankliniella desmodii. A) Body. B) Head and pronotum. C) Upper surface of a hind coxa. D) Abdominal tergite VIII posteromarginal comb.
Figure 37. A–D in The identification of the flower thrips, Frankliniella Karny (Thysanoptera: Thripidae) intercepted at U.S. ports of entry
Figure 37. A–D) Frankliniella intonsa. A) Body. B) Head and pronotum. C) Metanotum. D) Abdominal tergite VIII posteromarginal comb.
Figure 25. A–C in The identification of the flower thrips, Frankliniella Karny (Thysanoptera: Thripidae) intercepted at U.S. ports of entry
Figure 25. A–C) Frankliniella cubensis. A) Body. B) Left and right pedicel of antennal segments III. C) Head and pronotum.
Figure 46. A–C in The identification of the flower thrips, Frankliniella Karny (Thysanoptera: Thripidae) intercepted at U.S. ports of entry
Figure 46. A–C) Frankliniella pelucensis, holotype, USNM 01207643. A) Body. B) Head and pronotum. C) Abdominal ter-
Figure 24. A–D in The identification of the flower thrips, Frankliniella Karny (Thysanoptera: Thripidae) intercepted at U.S. ports of entry
Figure 24. A–D) Frankliniella cognata, holotype, USNM 74430. A) Body. B) Dorsal subapical region of antennal
Figure 53. A–D in The identification of the flower thrips, Frankliniella Karny (Thysanoptera: Thripidae) intercepted at U.S. ports of entry
Figure 53. A–D) Frankliniella tuberosi. A) Body. B) Head and pronotum. C) Abdominal tergite I. D) Abdominal tergite VIII posteromarginal comb.
Figure 21. A–E in The identification of the flower thrips, Frankliniella Karny (Thysanoptera: Thripidae) intercepted at U.S. ports of entry
Figure 21. A–E) Frankliniella brunnea. A) Body. B) Ventral eye. C) Pedicel of antennal segment III. D) Head and pronotum. E) Upper surface of a hind coxa.
Figure 20. A–D in The identification of the flower thrips, Frankliniella Karny (Thysanoptera: Thripidae) intercepted at U.S. ports of entry
Figure 20. A–D) Frankliniella bruneri A) Body. B) Antennal segments VI–VIII. C) Head and pronotum. D) Upper surface
Figure 28. A–C in The identification of the flower thrips, Frankliniella Karny (Thysanoptera: Thripidae) intercepted at U.S. ports of entry
Figure 28. A–C) Frankliniella fallaciosa A) Body. B) Head and pronotum. C) Upper surface of a hind coxa. Arrow indicates a microtrichium.
Figure 23. A–D in The identification of the flower thrips, Frankliniella Karny (Thysanoptera: Thripidae) intercepted at U.S. ports of entry
Figure 23. A–D) Frankliniella citripes, holotype, USNM 74422. A) Body. B) Dorsal subapical region of antennal segment VI. C) Head and pronotum. D) Upper surface of a hind coxa. Arrows indicate microtrichia.
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OpenNeuro
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