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11 results for “transmission electron microscope”
Three-dimensional Reconstructions and Quantitative Indicators for colloidal particles in Dry and Liquid Conditions in Scanning Transmission Electron Microscope (STEM)
<p>This dataset accompanies the research presented in the paper:</p> <div>Esteban, D.A., Wang, D., Kadu, A., Olluyn, N., Iglesias, A.S., Perez, A.G., Casablanca, J.G., Nicolopoulos, S., Liz-Marzán, L.M. and Bals, S., 2023. Liquid phase fast electron tomography unravels the true 3D structure of colloidal assemblies. <em>arXiv preprint arXiv:2311.05309</em>. [<a href="https://arxiv.org/pdf/2311.05309" target="_blank" rel="noopener">link</a>]</div> <p>It provides a comprehensive collection of three-dimensional reconstructions and quantitative descriptors for small colloidal particles. These gold nanoparticles are arranged in tetrahedral and other intricate geometries under both dry and liquid conditions. The dataset contains 3D reconstructions and quantitative indicators such as centroids, volumes, surface areas, solidity measures, and principal axis lengths for assemblies with 4, 5, and 6 particles. </p> <p>The dataset includes: <code>N4_dry_dart.rec</code> and <code>N4_liquid_dart.rec</code> for the 3D reconstructions of an assembly with 4 particles in dry and liquid conditions respectively; <code>N4_quant_descriptors_dry.mat</code> and <code>N4_quant_descriptors_liquid.mat</code> providing quantitative descriptors for these conditions. Similar files are provided for assemblies with 5 and 6 particles, such as <code>N5_dry_dart.rec</code>, <code>N5_liquid_dart.rec</code>, <code>N5_quant_descriptors_dry.mat</code>, <code>N5_quant_descriptors_liquid.mat</code>, and the corresponding files for N6. </p> <p>This dataset can be used to study the structural dynamics of nanoparticle assemblies and studies in colloidal chemistry, materials science, and nanotechnology. The <code>.rec</code> files can be visualized using volume rendering software (e.g. Amira or Avizo), while the <code>.mat</code> files contain structured data for analysis in MATLAB. The supporting code and scripts for this dataset are available on the GitHub repository: <a href="https://github.com/ajinkyakadu/LiquidET_NatComm2024" target="_new" rel="noreferrer">https://github.com/ajinkyakadu/LiquidET_NatComm2024</a>. </p>
Supporting data for "In situ Quantitative Tensile Tests on Antigorite in a Transmission Electron Microscope"
<p>Abstract: The determination of the mechanical properties of serpentinites is essential towards the understanding of the mechanics of faulting and subduction. Here, we present the first in situ tensile tests on antigorite in a transmission electron microscope. A push-to-pull deformation device is used to perform quantitative tensile tests, during which force and displacement are measured, while the microstructure is imaged with the microscope. The experiments have been performed at room temperature on beams prepared by focused ion beam. The specimens are not single crystals despite their small sizes. Orientation mapping indicated that some grains were well-oriented for plastic slip. However, no dislocation activity has been observed even though engineering tensile stress went up to 700 MPa. We show also that antigorite does not exhibit an pure elastic-brittle behaviour since, despite the presence of defects, the specimens underwent plastic deformation and did not fail within the elastic regime. Instead, we observe that strain localizes at grain boundaries. All observations concur to show that under our experimental conditions, grain boundary sliding is the dominant deformation mechanism. This study sheds a new light on the mechanical properties of antigorite and calls for further studies on the structure and properties of grain boundaries in antigorite and more generally in phyllosilicates.</p>
Supplementary material for: Calibrating coordinate system alignment in a scanning transmission electron microscope using a digital twin.
<h1>Calibrating coordinate system alignment in a scanning transmission electron microscope using a digital twin.</h1> <h2>Supplementary material</h2> <p>This deposition contains supplementary material for a paper on coordinate system calibration in 4D STEM. A preprint of the paper is available at <a href="https://arxiv.org/abs/2403.08538">https://arxiv.org/abs/2403.08538</a>.</p> <h2>Contents</h2> <div> <div><code>20221025_154811.zip</code>: Overfocused 4D STEM test dataset</div> <div> </div> <div><code>overfocus.sif</code>: Apptainer image with complete software stack. <code>apptainer run --writable overfocus.sif</code> to execute. It starts a Jupyterlab instance with two notebooks, one to genreate test data and the other to perform the interactive adjustment. This documents the software version that was used for the figures in the paper.</div> <div> </div> <div><code>requirements.txt</code>: Python package versions of dependencies in <code>overfocus.sif</code>. </div> <div> </div> <div><code>COM - Jupyter Notebook - Google Chrome 2023-01-25 12-40-07_processed.mp4</code>: Screen capture video with explanation of the first live calibration with an early prototype.</div> <div> </div> <div><code>video description.docx</code>: Explanation of the plots and adjustment process in the screen capture video.</div> <div> </div> <div><code>Microscope-Calibration.tar.gz</code>: Repository archive of the software and examples for calibration in the version used in the paper.</div> <div> </div> <div><code>TemGym.tar.gz</code>: Repository archive of TemGym Basic in the version used in the paper.</div> </div>
Atom Probe Tomography performed in a transmission electron microscope (JEOL F 200)
<p>Material: Fe-51.4at% Cr processed by high pressure torsion<br>Temperature of analysis: 78 K<br>Pulse repetition rate: 20 kHz<br>APT detector type: advanced delay line detector<br>Number of atoms collected: 1.235 millions<br>Volume size: 9 x 9 x 93 nm3</p>
Coherent light emission in cathodoluminescence when using GaAs in a scanning (transmission) electron microscope_experimental dataset
<p>This dataset contains the raw unprocessed experimental data for the "Coherent light emission in cathodoluminescence when using GaAs in a scanning (transmission) electron microscope" by Michael Stöger-Pollach et al., Ultramicroscopy 224 (2021) 113260. </p>
Transmission electron microscope images dataset for AutoDetect-mNP (triangular prisms)
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Transmission electron microscope images dataset for AutoDetect-mNP (nanorods)
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Fig. 6 Cnidosacs and nematocysts. a–d Transmission electron microscopic images, longitudinal sections. a in Pseudovermis paradoxus 2.0-3D microanatomy and ultrastructure of a vermiform, meiofaunal nudibranch (Gastropoda, Heterobranchia)
Fig. 6 Cnidosacs and nematocysts. a–d Transmission electron microscopic images, longitudinal sections. a Overview of an entire cnidosac. b Close-up of nematocysts. c Intact nematocyst. d Degraded nematocyst and muscular lining. clw cell wall, cp cnidophage, cw capsule wall, dnc degraded nematocyst, fi filament thread, ha harpoon, inc intact nematocyst, lu lumen, m mitochondrion, ml muscular lining, mv microvilli, nc nematocyst, nu nucleus, vac vacuole
Block scanning strain measurement in Transmission electron microscope
<p>This data set and the processing script is part of the publication "Smart HAADF-STEM scanning strategy for local measurement of strain at the nanoscale". The data set contains the 64x64 and 32x32 pixel subimage scanning along with the Bessel diffraction data. It is a comparison of the new scanning strategy called the "Block scanning" with that of the Bessel diffraction technique. The experiments were performed with the FEI Titan microscope operating at 300 kV high tension.The python script allows the extraction of the strain from the block scanning technique. The Bessel diffraction processing software can be found in the link: <a href="https://bitbucket.org/lutosensis/tem-thesis/src/master/">https://bitbucket.org/lutosensis/tem-thesis/src/master/</a></p>
Visualizing sub-atomic orbital and spin moments using a scanning transmission electron microscope: Data and Methodology
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Light and transmission electron microscopic images for quantitative lung morphology of mechanically ventilated rat lungs.
<p>The joint American Thoracic Socity (ATS) and European Respiratory Society (ERS) guidelines recommend design-based stereology for quantitative morphology of lung structures in health and disease (DOI:<a href="https://doi.org/10.1164/rccm.200809-1522ST"> 10.1164/rccm.200809-1522ST</a>). Design-based stereology is founded on stochatic geometry and rigorous sampling protocols to obtain a set of images which is representative for the whole organ so that the generated data are accurate (unbiased) and sufficiently precise. Regarding quantitative light and electron microscopic analyses lungs were fixed by airway instillation fixation using 1.5 % glutaraldehyde, 1.5% paraformaldehyde in 0.15M HEPES buffer at a hydrostatic pressure of 25 cmH<sub>2</sub>O. Afterwards lungs were subjected to a systematic uniform random sampling for light and transmission electron microscopy based on established protocols (DOI: 10.1016/j.aanat.2013.04.011). Regarding light microscopy, 3 to 4 tissue samples were taken randomly per lung, embedded in plastic (Technovit 8100), sectioned (thickness of 1.5 µm) and stained with toluidine blue. Whole slides were scanned (AxioScanZ.1) and subjected to a systematic uniform area sampling process using newCAST-stereology software (Visiopharm, Hoersholm, Denmark) to image 10% of the section. Depending on the size of the section the goal was to obtain at least 60 (in general >100) randomized light microscopic images per lung for quantitative assessment. Moreover, 6 tissue blocks were randomly taken for transmission electron microscopy, embedded in epoy resin (Epon) and sectioned with a thickness of 80nm. Using an electron microscope (FEI Morgagni transmission electron microscope, Eindhoven, The Netherlands), a systematic uniform area samplinbg was performed and at least 100 micrographs were randomly taken per lung. The dataset in this publication contains the representative light and transmission electron microscopic images of lungs from six different experimental groups (male Fisher 344 rats, aged 11-13 weeks). Healthy rat lung were either not mechanically ventilated (H/ No ventil) or mechanically ventilated with positive end-exspiratory pressure (PEEP) = 1 cmH2O (group H/PEEP1) or PEEP = 5 cmH2O (group H/PEEP5). In addition, lungs suffering from occult lung injury due to bleomycin instillation were also either not mechanically ventilated (B/No ventil) or ventiled with PEEP = 1 cmH2O (B/PEEP1) or PEEP = 5 cmH2O (B/PEEP5). The respiratory rate was 90/min and the tidal volume was 10ml/ kg bodyweight.</p>
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International Brain Laboratory public data
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OpenNeuro
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