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1,076 results for “uptake”
Organic and Inorganic Nitrogen Uptake by Sarracenia Purpurea at Harvard Forest and Fort Albany ON 2007
The nitrogen-limited carnivorous pitcher plant Sarracenia purpurea and its associated detritus-based food web is a model system for studying plant nutrient dynamics. We tested if S. purpurea can directly take up intact amino acids and compared uptake of organic and inorganic forms of nitrogen (N) across a gradient of N deposition. At sites in Canada and the United States, individual pitchers with complete or incomplete food webs were fed U-13C-15N-glycine, U-13C-15N-phenylalanine and 15NH415NO3 individually and in mixture. Plants took up intact amino acids. Acquisition of each N form provided in isolation exceeded uptake of the same form in mixture. At the high deposition site, uptake of 15N from amino acids was higher than uptake of 15N from inorganic nitrogen. At the low deposition site, uptake of 15N from all three forms of N were similar. Completeness of the associated food web had no effect on 15N uptake. By taking up intact amino acids, Sarracenia purpurea can short-circuit the inorganic N cycle, thus minimizing potential bottlenecks in N availability that result from the plant’s reliance for nitrogen mineralization on a seasonally reconstructed food web operating on infrequent and irregular prey capture.
PARAGON 1 - KM2112 - Iron Uptake In Situ Measurements
<p>This dataset contains measurements of in situ rates of dissolved iron (Fe) uptake collected during the PARAGON 1 expedition (KM2112) in the North Pacific Subtropical Gyre. Measurements come from daily collections of whole seawater at 150 m using trace metal clean techniques. Rates of iron uptake were measured by subsampling 250 mL aliquots of whole seawater into polycarbonate bottles and spiking with a final concentration of 0.4 nmol L⁻¹ ⁵⁵FeCl₃ with a specific activity of 2.74 Ci mmol⁻¹ of Fe (Perkin Elmer). A killed blank measurement was also made by spiking an additional 250 mL aliquot with a final concentration of 1% glutaraldehyde prior to spiking with ⁵⁵FeCl₃. All aliquots were then incubated in the dark at in situ temperature for 8-10 hours. Incubations were terminated by filtering the entire 250 mL aliquot for each sample through a 0.2 µm polycarbonate filter. In order to remove extracellularly bound Fe, filters were rinsed 3 times with an oxalate wash according to Tang and Morel (2006) followed by three rinses with 0.2 µm filtered seawater. Filters were then transferred to high density polyethylene scintillation vials and submerged in 10 mL of Ultima Gold LLT scintillation cocktail (Perkin Elmer). Radioactivity incorporated into microbial biomass was measured on a TriCarb 4910TR scintillation counter. Values reported are blank-corrected averages of triplicate measurements from each sample but have not been adjusted for isotope dilution resulting from unlabeled iron present in situ. Timestamp is in UTC.</p>
PARAGON 1 - KM2112 - Iron Uptake Timecourse Incubations
<p>This dataset contains measurements of rates of dissolved iron (Fe) uptake collected during the PARAGON 1 expedition (KM2112) in the North Pacific Subtropical Gyre. Measurements come from time-course incubation experiments initiated with whole seawater collected at 150 m using trace metal clean techniques and modified with various additions of organic carbon, iron, and/or nitrogen. Rates of iron uptake were measured at multiple time points for each experimental biological replicate by subsampling 250 mL aliquots from polycarbonate incubation bottles into new polycarbonate bottles and spiking with a final concentration of 0.4 nmol L⁻¹ ⁵⁵FeCl₃ with a specific activity of 2.74 Ci mmol⁻¹ of Fe (Perkin Elmer). A killed blank measurement was made for each experimental treatment by spiking an additional 250 mL aliquot with a final concentration of 1% glutaraldehyde prior to spiking with ⁵⁵FeCl₃. All aliquots were then incubated in the dark at in situ temperature for 8-10 hours. Incubations were terminated by filtering the entire 250 mL aliquot for each sample through a 0.2 µm polycarbonate filter. In order to remove extracellularly bound Fe, filters were rinsed 3 times with an oxalate wash according to Tang and Morel (2006) followed by three rinses with 0.2 µm filtered seawater. Filters were then transferred to high density polyethylene scintillation vials and submerged in 10 mL of Ultima Gold LLT scintillation cocktail (Perkin Elmer). Radioactivity incorporated into microbial biomass was measured on a TriCarb 4910TR scintillation counter. Values reported are blank-corrected but have not been adjusted for isotope dilution resulting from unlabeled iron present in situ. Timestamp is in UTC. Version 2 corrects formatting errors in the timestamp.</p>
PARAGON 2 - KM2209 - Iron Uptake Timecourse Incubations
<p>This dataset contains measurements of rates of dissolved iron (Fe) uptake collected during the PARAGON 2 expedition (KM2209) in the North Pacific Subtropical Gyre. Measurements come from time-course incubation experiments initiated with whole seawater collected at 150 m using trace metal clean techniques and modified with various additions of organic carbon, iron, and/or nitrogen. Rates of iron uptake were measured at multiple time points for each experimental biological replicate by subsampling 250 mL aliquots from polycarbonate incubation bottles into new polycarbonate bottles and spiking with a final concentration of 0.4 nmol L⁻¹ ⁵⁵FeCl₃ with a specific activity of 2.74 Ci mmol⁻¹ of Fe (Perkin Elmer). A killed blank measurement was made for each experimental treatment by spiking an additional 250 mL aliquot with a final concentration of 1% glutaraldehyde prior to spiking with ⁵⁵FeCl₃. All aliquots were then incubated in the dark at in situ temperature for 8-10 hours. Incubations were terminated by filtering the entire 250 mL aliquot for each sample through a 0.2 µm polycarbonate filter. In order to remove extracellularly bound Fe, filters were rinsed 3 times with an oxalate wash according to Tang and Morel (2006) followed by three rinses with 0.2 µm filtered seawater. Filters were then transferred to high density polyethylene scintillation vials and submerged in 10 mL of Ultima Gold LLT scintillation cocktail (Perkin Elmer). Radioactivity incorporated into microbial biomass was measured on a TriCarb 4910TR scintillation counter. Values reported are blank-corrected but have not been adjusted for isotope dilution resulting from unlabeled iron present in situ. Timestamp is in UTC.</p>
Alteromonas macleodii MIT1002 growth on and uptake of Prochlorococcus-derived metabolites
This data package contains the results from a series of experiments designed to test the response of a heterotrophic, copiotrophic, gammaproteobacterium, Alteromonas macleodii strain MIT1002, to a range of metabolites released by the phytoplankton Prochlorococcus. A. macleodii MIT1002 was isolated from co-culture with Prochlorococcus, and so we hypothesized that A. macleodii MIT1002 would be able to grow on the full range of substrates tested. Instead, we found that A. macleodii MIT1002 could only grow on a narrow range of substrates, and data suggest that this substrate specificity may be related to transporter specificity. We performed two types of experiments: growth experiments and uptake experiments. Data from growth experiments are labeled with the name of the substrate being tested (e.g., “Leu,” for leucine, or “3m2ob”, for 3-methyl-2-oxobutanoic acid). For these experiments, we grew A. macleodii MIT1002 on either pyruvate (as a positive control), a selected Prochlorococcus-related substrate, or a mix of pyruvate and the metabolite. We measured growth in 96-well plates by OD600 using a plate reader which took a measurement every 0.5h for 48h. Uptake experiments are labeled with either “KHU7” (an experiment which tested A. macleodii MIT1002 growth on and uptake of 3-methyl-2-oxobutanoic acid) or “KHU8” (an experiment which tested the A macleodii MIT1002 growth on and uptake of 3-methyl-2-oxopentanoic acid -or lack thereof). For these experiments, we measured growth by flow cytometry. We measured the change in dissolved (i.e., extracellular) metabolite concentration by filtering samples, extracting organic carbon from the filtrate by solid phase extraction, and quantifying selected metabolites from the filtrate by targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS). This data package includes the peak areas for targeted metabolites generated by LC-MS/MS, the peak areas for our standard curves used for quantification, and the dissolved metabolite
Size-fractionated net primary productivity (NPP) estimates based on 13C uptake during cruises along the Northeast U.S. Shelf Long Term Ecological Research (NES-LTER) Transect, ongoing since 2019
This dataset consists of primary production measurements based on uptake of carbon-13 added as 13C-bicarbonate during 24-h deckboard incubations of seawater. Sampling occurred on cruises along the Northeast U.S. Shelf Long Term Ecological Research (NES-LTER) Transect during summer, fall, and winter, starting in summer 2019. Net primary production (NPP) was determined from particulate organic carbon (POC) content and associated stable isotope enrichment. Three data tables are included: 1. Depth-specific primary productivity based on fractional light levels of the surface irradiance with reference to the profile of photosynthetically active radiation (PAR). 2. Integrated primary productivity. 3. Natural abundance POC. The tables derive from the raw data included as other entities.
15N (Nitrate and Ammonium) Uptake by Spartina Alterniflora Sourced from Plum Island Estuary, MA and North Inlet, SC
15N uptake measurements were taken from Spartina alterniflora plants grown from seeds originating from six march locations in PLUM Island Estuary (PIE) and North Inlet, SC. All sites were Spartina alterniflora dominated marsh. 15N uptake was measured after 60 minutes in Nitrate, Ammonium, or control treatments. Seeds were collected in Fall 2022, reared Winter-Summer 2023, and uptake measured in Summer 2023.
Dataset for "Fertilizer value of dairy processing waste materials and contributions of soil microorganisms towards phosphorus uptake in grasses."
<p>This file includes the dataset used for the analysis of the fertilizer value from dairy processing waste materials and the contribution of soil microorganisms towards P uptake. More information in the linked future publication</p>
Global Ocean Heat Content Anomalies and Ocean Heat Uptake based on mapping Argo data using local Gaussian processes
<p>Monthly Ocean Heat Content Anomalies (OHCA) in the top 2000 dbar of the ocean are calculated (during 2004-2024, equatorward of 65 degree latitude) subtracting the mean over the period 2004-2024 from the monthly time series of OHC. Yearly OHCA time series are then calculated that include 1. one point per year, i.e., from averaging Jan to Dec (see files ending in “yearly.nc”), and 2. two points per year, i.e., from averaging Jan to Dec and Jul to Jun, respectively (see files ending in “yearly2.nc”). OHC fields are mapped using locally stationary Gaussian processes (defined over space and time) with data-driven decorrelation scales (Kuusela and Stein, 2018). A linear time trend was included in the estimate of the mean field (along with spatial terms and harmonics for the annual cycle). Mapping is done separately for different vertical sections: 15-20 dbar, 15-300 dbar, 300-700 dbar, 700-1850 dbar, 1800-1850 dbar. The 15-20 dbar (1800-1850 dbar) section is used to estimate OHCA for 0-15 dbar (1850-2000 dbar), where observations are sparser. Different vertical sections are combined to estimate global OHCA time series for 0-2000 dbar, 0-700 dbar, 700-2000 dbar (as indicated in the file names). The attribute "area" is included in the netcdf files and it tells the corresponding surface area for the estimates. Regions of the ocean that are shallower than 300 m or are not sufficiently well sampled by the Argo array are not included. Maps of the ocean masks used for the different vertical sections can be found in the .png files (blue shading indicates the area used for the horizontal integral); the bathymetry mask by Roemmich and Gilson (included in the file RG_ArgoClim_Temperature_2019.nc at https://sio-argo.ucsd.edu/RG_Climatology.html) is also used to define the ocean mask. Ocean Heat Uptake is calculated from the monthly OHCA and then averaged as described above to produce yearly time series included in the files for the different layers.</p> <p>For the uncertainty at each time point, the standard deviation of each OHCA/OHU value in the time series is included. When plotting a time series, the user may consider, e.g., shading plus/minus 1* or 1.96*standard deviation (corresponding to a confidence level of 68% or 95% respectively). These standard deviations in the files are estimated using spatially and temporally dependent conditional simulations of monthly gridded anomalies. When combining different layers, the standard deviation of the sum is conservatively estimated as the sum of the standard deviations. </p> <p>Finally, OHCA/OHU trends are estimated via a least-squares fit and reported in the variable metadata with uncertainties (confidence level of 68%). Trend uncertainties are estimated by repeating the fit for each member of the conditional simulation ensemble described above.</p> <p> </p>
Dataset: Open access potential and uptake in the context of Plan S - a partial gap analysis
<p>Dataset belonging to the report: <a href="https://doi.org/10.5281/zenodo.3543000">Open access potential and uptake in the context of Plan S - a partial gap analysis</a></p> <p> </p> <p>On the report: </p> <p>The analysis presented in the report, carried out by Utrecht University Library, aims to provide cOAlition S, an international group of research funding organizations, with initial quantitative and descriptive data on the availability and usage of various open access options in different fields and subdisciplines, and, as far as possible, their compliance with Plan S requirements.</p> <p>Plan S, launched in September 2018, aims to accelerate a transition to full and immediate Open Access. In the guidance to implementation, released in November 2018 and updated in May 2019, a gap analysis of Open Access journals/platforms was announced. Its goal was to inform Coalition S funders on the Open Access options per field and identify fields where there is a need to increase the share of Open Access journals/platforms. </p> <p>The report should be seen as a first step: an exploration in methodology as much as in results. Subsequent interpretation (e.g. on fields where funder investment/action is needed) and decisions on next steps (e.g. on more complete and longitudinal monitoring of Plan S-compliant venues) is intentionally left to cOAlition S and its members. </p> <p> </p> <p><em>This work was commissioned on behalf of cOAlition S by the Dutch Research Council (NWO), a member of cOAlition S. Bianca Kramer and Jeroen Bosman of Utrecht University Library were appointed to lead the project.</em></p>
Photos from WIDER UPTAKE case study site in the Czech Republic (Camera 1)
<p><span>Hourly photos from camera 1 at the WIDER UPTAKE H2020 project's case study site in the Czech Republic. </span></p>
Roadmapping information for each Wider Uptake case study
<p>Database summarizing information for each roadmapping step and for each of the WIDER UPTAKE H2020 project's case studies</p>
Photos from WIDER UPTAKE case study site in the Czech Republic (Camera 3)
<p>Hourly photos from camera 3 at the WIDER UPTAKE H2020 project's case study site in the Czech Republic.</p>
Photos from WIDER UPTAKE case study site in the Czech Republic (Camera 2)
<p>Hourly photos from camera 2 at the WIDER UPTAKE H2020 project's case study site in the Czech Republic.</p>
Combined data online measurements_WIDER UPTAKE
<p>Raw data from daily online measurements at the WIDER UPTAKE H2020 project's case study site in the Czech Republic.</p>
Novel estimates of the leaf relative uptake rate of carbonyl sulfide from optimality theory
<p>Data and Matlab scripts for repeating the analysis presented in the paper. In addition, global monthly climatological LRUs are provided at 0.05° resolution for the period 2001-2010 as nc-files. </p>
Leaf leachate chemistry and uptake metrics in headwater streams July 2015
This dataset contains the results of uptake experiments conducted at Caribou-Poker Creeks Research Watershed in July 2015. The objective of the study was to determine the relative influence of molecular composition and nutrient content of organic matter on the retention of dissolved organic matter (DOM) in boreal streams. We measured in situ rates of carbon uptake in streams following introduction of leachates derived from of alder (Alnus incana ssp. tenuifolia), poplar (Populus balsamifera), and white spruce (Picea glauca) trees subject to long-term fertilization with nitrogen (N) or phosphorus (P). We measured leachate composition through chemical analysis and optical properties (absorbance and fluorescence).
Stream nitrate concentrations and discharge, stream nitrate uptake, and results of stream network nitrate model to determine lateral nitrate load from land to stream in Oak Creek, Arizona, USA
Data package associated with Handler et al. (2024) "Nitrate loads from land to stream are balanced by in-stream nitrate uptake across season in a dryland stream". The study describes the nitrate dynamics in Oak Creek watershed. Data include measurements from four seasonal synoptic sampling campaigns, nine seasonal stream nitrate uptake experiments on the main stem and tributaries, and the results of a network model that estimates the lateral load of nitrate from surrounding landscape to the stream network as well as network-level stream nitrate uptake and retention.
EJR01 Foraging decisions underlying restricted space-use: effects of fire and forage maturation on large herbivore nutrient uptake on Konza Prairie
Recent models suggest that herbivores optimize nutrient intake by selecting patches of low to intermediate vegetation biomass. We assessed the application of this hypothesis to plains bison (Bison bison) in an experimental grassland managed with fire by estimating daily rates of nutrient intake in relation to grass biomass and by measuring patch selection in experimental watersheds in which grass biomass was manipulated by prescribed burning. Digestible crude protein content of grass declined linearly with increasing biomass, and the mean digestible protein content relative to grass biomass was greater in burned watersheds than watersheds not burned that spring (intercept; F1,251 = 50.57, P < 0.0001). Linking these values to published functional response parameters, ad libitum protein intake, and protein expenditure parameters, Fryxell's (Am. Nat., 1991, 138, 478) model predicted that the daily rate of protein intake should be highest when bison feed in grasslands with 400 - 600 kg/ha. In burned grassland sites, where bison spend most of their time, availability of grass biomass ranged between 40 and 3650 kg/ha, bison selected foraging areas of roughly 690 kg/ha, close to the value for protein intake maximization predicted by the model. The seasonal net protein intake predicted for large grazers in this study suggest feeding in burned grassland can be more beneficial for nutrient uptake relative to unburned grassland as long as grass regrowth is possible. Foraging site selection for grass patches of low to intermediate biomass help explain patterns of uniform space use reported previously for large grazers in fire-prone systems. This data set was used to test the forage maturation hypothesis in the Konza Prairie bison enclosure from 2012-2013. Our objectives were to quantify foraging site selection of Plains bison in order to determine if bison in a fire-prone grassland selected sites of low-to-intermediate forage biomass as posited by Fryxell’s (1991) forage mat
Thymidine uptake rates for estimating bacterial production in lakes, McMurdo Dry Valleys, Antarctica (1993-2023, ongoing)
An important part of the McMurdo Long Term Ecological Research (LTER) is monitoring of spatial and temporal patterns, and processes that control bacterial production in perennial ice-covered lakes. This data set quantifies thymidine (TdR) uptake by bacteria, which can be used to estimate bacterial production.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.