C. elegans Locomotion Video Checklist
Generate a printable filming protocol with microscope optics, locomotion types, apparatus, acclimation notes, and standardized file naming for C. elegans crawling, swimming, and behavioral studies or ConductVision upload.
Try it out
Load example C. elegans locomotion video checklist data to see the full workflow
Session Info
Locomotion Types
Camera / Optics
Apparatus
Filming Protocol (0 clips)
File naming: _day1_adult_2026-04-29_[LOCO]_[CAMERA]_[APPARATUS]_trial[N]
Select locomotion types, camera/optics, and apparatus to generate your protocol.
Analyze your C. elegans locomotion videos with ConductVision
Upload your standardized clips for AI-powered locomotion analysis, body bend quantification, and behavioral scoring.
Go to ConductVisionFilming protocol follows established C. elegans locomotion methodologies (WormTracker, Tierpsy, CeleST). Upload completed videos to ConductVision for AI locomotion analysis.
How It Works
Select the developmental stage (L1–L4, young adult, day-1 adult, aged), locomotion types (crawling, swimming, omega turns, chemotaxis, thermotaxis, touch response), microscope optics (stereoscope, compound, dark field, DIC), and apparatus (NGM plates, liquid wells, microfluidic chips, gradient plates, multi-worm tracker). Optionally enable high-magnification imaging, acclimation protocols, and food condition tracking. The tool generates a numbered shot list with C. elegans–specific instructions and duration. Incompatible combinations (e.g. swimming without liquid well) are automatically filtered.
Filming Best Practices
Use synchronized populations — L4 or day-1 adult is standard for most locomotion assays. Seed NGM plates with OP50 24 hours before use. Maintain 20°C for N2 wild-type; adjust for temperature-sensitive mutants. Pick worms gently: rough picking alters locomotion for several minutes. Equilibrate plate temperature before opening to avoid condensation that traps worms. Frame rate: 5–15 fps is sufficient for crawling speed and body bends; 30 fps for detailed omega turn analysis. For swimming/thrashing assays, count body bends per 30-second interval in M9 buffer. Use plates poured 1–3 days prior — old plates crack and dehydrate.