Enter baseline and post-conditioning chamber times, compute delta CPP, post preference percent, centre-time QC, group SEM, and CSV export.
Enter baseline and post-conditioning chamber times. Delta CPP corrects post-test preference for each animal's pre-conditioning side bias.
| Animal | Group | Pre drug side (s) | Pre saline side (s) | Pre centre (s) | Post drug side (s) | Post saline side (s) | Post centre (s) | QC |
|---|---|---|---|---|---|---|---|---|
| OK | ||||||||
| OK | ||||||||
| OK |
| Animal | Group | CPP pre | CPP post | Delta CPP | Post preference |
|---|---|---|---|---|---|
| M1 | vehicle | 30.0 | 50.0 | 20.0 | 53.1% |
| M2 | cocaine | -5.0 | 400.0 | 405.0 | 72.2% |
| M3 | cocaine | -30.0 | 320.0 | 350.0 | 67.8% |
When to use
Do not use for
A post-only preference score can mistake initial side preference for conditioning. Delta CPP keeps the baseline chamber bias in the calculation.
Drug-paired side should be balanced across animals whenever the design allows. A confounded side assignment can look like reward even when it is apparatus preference.
Centre time should not change dramatically between baseline and post tests. Large drift can suggest tracking or behavior changes that make side preference harder to interpret.
CPP score is computed as drug-paired side time minus saline-paired side time. Delta CPP is post CPP minus pre CPP. Post preference percent is drug-side post time divided by drug-side plus saline-side post time. Centre-time drift is flagged when post centre time differs from pre centre time by more than 15%. Group SEM uses sample standard deviation divided by square root of n.
Last validated 2026-04-30. Calculations are designed for planning and documentation support; verify procurement decisions against manufacturer specifications or institutional SOPs.
ConductScience Conditioned Place Preference Analyzer (v1.0). ConductScience, Inc. 2026. Available at: https://conductscience.com/tools/conditioned-place-preference-analyzer
This tool performs descriptive calculations from user-entered CPP times. It does not replace protocol-specific statistics, exclusion criteria, or animal welfare oversight.
Conditioned place preference is a context-reward learning assay. The animal explores a multi-compartment chamber, then receives drug-paired and control-paired conditioning sessions in distinct compartments. In the post-conditioning test, increased time in the drug-paired side indicates conditioned approach to the drug-associated context.
This analyzer treats the baseline and post tests as paired observations for each animal. It reports the raw pre and post CPP scores plus the baseline-corrected delta CPP endpoint.
CPP pre equals pre-conditioning drug-side time minus saline-side time. CPP post equals post-conditioning drug-side time minus saline-side time. Delta CPP equals CPP post minus CPP pre.
Post preference percent is drug-side post time divided by drug-side plus saline-side post time, multiplied by 100. Group summaries use animal-level delta CPP values and report mean plus SEM.
Counterbalance side assignment, verify that the apparatus has no strong unconditioned side bias, and keep chamber cues, lighting, cleaning, and test duration fixed across groups.
Review animals with large centre-time drift before final analysis. A major centre-time change can indicate tracking-zone changes, freezing, obstruction, or altered exploration that affects side preference.
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