Cylinder Test Scorer

Score left, right, and both-paw wall contacts, map lesion side, calculate Schallert asymmetry index, and export animal-level CSV.

Asymmetry IndexLesion SideCSV Export

Cylinder contact table

Enter left-only, right-only, both-paw, and optional no-contact events. Lesion side determines ipsilateral and contralateral assignment automatically.

AnimalGroupLesion sideLeft onlyRight onlyBoth pawsNoneContacts
22
21
20

Animal summary

AnimalGroupIpsiContraBothAsymmetry indexLeft use
M1sham9850.04540.9%
M26OHDA14340.52466.7%
M36OHDA13250.55010.0%

Group mean asymmetry index

  • Compute Schallert forelimb asymmetry from left, right, and both-paw contacts
  • Automatically map lesion side to ipsilateral and contralateral paws
  • Flag animals below the default 20-contact threshold
  • Export animal-level cylinder scoring data for Prism, R, or Python
  • Pair cylinder asymmetry with rotarod, pole, beam walk, and wire hang endpoints

Don't use for

  • Gait stance or swing kinematics from runway tracking
  • Grip strength force measurements
  • Bilateral motor impairment where no lesion side is meaningful

Resources

  • Lesion side confirmed for each animal
  • Wall-touch definition written before scoring
  • At least 15 to 20 contacts targeted per animal
  • Cylinder cleaned between animals
  • Lighting and reflections controlled
  • Observer blinded to group when possible

What Is the Cylinder Test?

The cylinder test is a spontaneous forelimb-use assay. A rodent is placed in a transparent cylinder and the observer scores wall contacts made with the left paw, right paw, or both paws during vertical exploration.

Because unilateral motor lesions often reduce use of the contralateral forelimb, the assay is widely used in 6-OHDA, MCAO stroke, and other lateralized sensorimotor models.

Metrics and Math

This scorer assigns ipsilateral and contralateral contacts from the lesion side. It computes the Schallert asymmetry index as (ipsilateral - contralateral) / (ipsilateral + contralateral + both). It also reports percent left use as left-only contacts divided by all left, right, and both-paw contacts.

None events are exported for audit context but are not included in the asymmetry denominator.

Best Practices

Predefine contact rules, score enough contacts per animal, and blind observers to treatment or lesion status when possible. Clean the cylinder between animals and avoid reflections or lighting artifacts that make paw contact calls ambiguous.

Balance testing time and habituation because arousal and exploratory drive can change the number of usable contacts.

Frequently Asked Questions