Advanced Cryostat Microtome with Touchscreen & 5-Zone Cooling
This advanced cryostat microtome delivers ultra-precise frozen tissue sectioning for histology, pathology, and biomedical research labs requiring consistent, reproducible results. Equipped with independent 5-zone semiconductor cooling, a large 55×...
| Automation Level | manual |
| Sectioning Thickness Range | 0.5–100 μm |
| Trimming Thickness Range | 1–600 μm |
| Vertical Stroke | 66 mm |
| Maximum Specimen Size | 55×80 mm |
| Cooling Technology | 5-zone semiconductor (Peltier + compressor) |
At the heart of this cryostat microtome is a robust 5-zone independent semiconductor cooling system capable of driving the specimen temperature down to −65°C and maintaining the chamber at a stable −50°C. This level of thermal precision ensures optimal tissue preservation and section quality across a wide range of sample types. The generously sized 55×80 mm specimen window provides excellent visibility into the cutting area, while 24 quick-freeze points and 6 dedicated semiconductor stage points give researchers flexible, rapid specimen preparation without compromising temperature uniformity.
Workflow efficiency is dramatically enhanced by the 10.1-inch color touchscreen, which places all critical parameters—section thickness, feed speed, and temperature zones—within a single intuitive interface. Dual-speed feed control allows operators to switch seamlessly between coarse trimming and fine sectioning modes, reducing total preparation time. Built-in UVC disinfection provides automated chamber sterilization between samples, supporting strict biosafety protocols, while the heated anti-fog glass window maintains a clear line of sight throughout long operating sessions, minimizing errors and fatigue.
Sectioning flexibility spans 0.5 to 100 μm for fine diagnostic and research sections, complemented by a trimming range of 1 to 600 μm for rapid block face preparation. A 66 mm vertical stroke accommodates diverse specimen geometries, making this instrument equally suited to small biopsy cores and larger tissue blocks. Certified to CE and ISO 13485 standards and operating at a quiet 50 dB(A), it meets the regulatory and environmental demands of modern clinical and research laboratories.
| Specification | Value |
|---|---|
| Sectioning Thickness Range | 0.5–100 μm |
| Trimming Thickness Range | 1–600 μm |
| Specimen Retraction | 0–60 μm (2 μm increments) |
| Vertical Stroke | 66 mm |
| Specimen Window | 55×80 mm |
| Cooling Technology | 5-zone independent semiconductor (Peltier + SECOP compressors) |
| Min. Specimen Temperature | −65°C |
| Freezing Chamber Range | 0°C to −50°C |
| Quick-Freeze Points | 24 (+ 6 semiconductor stage points) |
| Display | 10.1-inch color touchscreen, multilingual |
| Disinfection | UVC ultraviolet |
| Noise Level | 50 dB(A) |
| Power | 500 W |
| Voltage | AC220V±10% 50 Hz (standard); AC110V±10% 60 Hz |
| Net Weight | 180 kg |
| Dimensions (L×W×H) | 700×780×1230 mm |
| Certifications | CE / ISO 13485 |
| Warranty | 1 year + lifetime technical support |
How It Works
The cryostat operates by maintaining a controlled freezing environment within an insulated chamber, typically cooled by a refrigeration system using Peltier elements or compressor-based cooling. Tissue specimens are rapidly frozen using optimal cutting temperature (OCT) embedding compound, which solidifies at sub-zero temperatures to provide structural support without chemical fixation.
The sectioning mechanism employs a precision microtome integrated within the freezing chamber, allowing for consistent section thickness control typically ranging from 1-100 micrometers. The specimen holder and cutting blade are maintained at optimal temperatures to prevent ice crystal formation that could damage cellular architecture. Anti-roll plates and section collection systems facilitate transfer of cut sections to glass slides.
Temperature regulation systems maintain separate zones for specimen storage, cutting area, and blade cooling, ensuring optimal conditions for different tissue types and cutting requirements. This controlled environment preserves cellular morphology, antigen epitopes, and enzymatic activity that may be compromised by chemical fixation or elevated temperatures.
Features & Benefits
Automation Level
- manual
Sectioning Thickness Range
- 0.5–100 μm
Trimming Thickness Range
- 1–600 μm
Vertical Stroke
- 66 mm
Maximum Specimen Size
- 55×80 mm
Cooling Technology
- 5-zone semiconductor (Peltier + compressor)
Min. Specimen Temperature
- −65°C
Freezing Chamber Range
- 0°C to −50°C
Quick-Freeze Points
- 24 + 6 semiconductor stage
Display
- 10.1-inch color touchscreen
Disinfection
- UVC ultraviolet
Noise Level
- 50 dB(A)
Net Weight
- 180 kg
Dimensions
- 700×780×1230 mm
Certifications
- CE / ISO 13485
Research Domain
- Cancer Research
- Cell Biology
- Clinical Diagnostics
- Developmental Biology
- Histopathology
- Neuroscience
Weight
- 36.71 lbs
Dimensions
- L: 123.0 in
- W: 70.0 in
- H: 78.0 in
Comparison Guide
| Feature | This Product | Typical Alternative | Advantage |
|---|---|---|---|
| Temperature Control Range | Consult product datasheet for specific temperature range | Basic models often provide limited temperature range with less precise control | Broader temperature range accommodates diverse tissue types and optimal cutting conditions for different applications. |
| Section Thickness Control | Consult product datasheet for thickness range and increment precision | Entry-level instruments may offer coarser thickness increments | Fine thickness control enables precise sectioning for specialized applications like serial reconstructions. |
| Chamber Design | Consult product specifications for chamber configuration | Smaller chambers limit specimen size and workflow efficiency | Optimized chamber design improves specimen accessibility and sectioning workflow for high-throughput applications. |
| Temperature Zones | Consult product datasheet for zone control capabilities | Single-zone systems provide less optimal cutting conditions | Independent temperature control optimizes specimen, blade, and chamber conditions simultaneously. |
This cryostat provides precision sectioning capabilities with controlled temperature environments essential for preserving tissue integrity during frozen section preparation. The instrument offers the temperature control and sectioning precision required for demanding histopathological and research applications.
Practical Tips
Verify temperature readings monthly using calibrated thermometers placed at specimen and blade positions.
Why: Temperature accuracy directly affects section quality and antigen preservation in immunostaining protocols.
Clean chamber surfaces daily with appropriate disinfectants and remove all tissue debris before storage.
Why: Residual organic matter can harbor contaminants and affect subsequent specimen processing quality.
Allow adequate equilibration time between different temperature settings before beginning sectioning.
Why: Temperature stability prevents artifacts and ensures consistent sectioning performance across specimens.
If sections curl or compress, adjust blade angle and verify optimal specimen temperature for tissue hardness.
Why: Proper blade geometry and temperature matching prevent sectioning artifacts that compromise morphological analysis.
Document temperature settings and section thickness for each specimen to ensure reproducible protocols.
Why: Standardized parameters enable consistent results across experiments and facilitate protocol optimization.
Use appropriate personal protective equipment including cut-resistant gloves when handling blades and frozen specimens.
Why: Sharp blades and extremely cold surfaces present injury risks requiring proper safety precautions.
Setup Guide
What’s in the Box
- Cryostat main unit (typical)
- Specimen holders and chucks (typical)
- Microtome blade holder (typical)
- Anti-roll plate system (typical)
- OCT embedding compound (typical)
- User manual and operation guide (typical)
- Calibration certificates (typical)
- Basic maintenance tools (typical)
Warranty
ConductScience provides a comprehensive one-year manufacturer warranty covering parts and labor, with technical support for installation, calibration, and troubleshooting to ensure optimal instrument performance.
Compliance
References
Background reading relevant to this product:
What section thickness range can be achieved with this cryostat?
Typical cryostats provide section thickness control from 1-100 micrometers with fine incremental adjustment. Optimal thickness depends on tissue type and intended staining protocol - consult product specifications for precise thickness capabilities and increment settings.
How does temperature control affect section quality?
Proper temperature control prevents ice crystal formation and maintains tissue architecture. Specimen temperature should match tissue hardness requirements, while blade cooling reduces section curling and improves cutting consistency.
What types of embedding media are compatible?
OCT (Optimal Cutting Temperature) compound is the standard embedding medium, providing structural support while preserving antigen integrity. Some instruments accommodate alternative embedding media - verify compatibility before use.
How frequently does the instrument require calibration?
Temperature calibration should be verified monthly or per institutional protocols using calibrated thermometers. Section thickness accuracy should be checked with micrometer standards when precision requirements change.
What maintenance is required for consistent performance?
Daily cleaning includes debris removal and chamber decontamination. Weekly maintenance involves blade inspection and temperature verification. Monthly tasks include refrigeration system checks and thorough decontamination protocols.
How does this compare to paraffin sectioning methods?
Cryosectioning provides rapid processing without chemical fixation, preserving enzymatic activity and heat-labile antigens. Processing time is minutes versus hours for paraffin embedding, though section morphology may be less optimal than paraffin sections.
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