Supplement of manuscript 521330

<p>This folder contains analysis data from the study submitted as manuscript (Manuscript ID 521330):</p> <p>TITLE:</p> <p>Comparative metabarcoding and metatranscriptomic analysis of microeukaryotes within coastal surface waters of West Greenland and Northwest Iceland</p> <p>AUTHORS:</p> <p>Stephanie Elferink, <a href="mailto:Stephanie.westphal@awi.de"> Stephanie.westphal@awi.de</a>, Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research&nbsp;</p> <p>Sylke Wohlrab, <a href="mailto:Sylke.wohlrab@awi.de"> Sylke.wohlrab@awi.de</a>, Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, and Helmholtz Institute for Functional Marine Biodiversity</p> <p>Stefan Neuhaus, <a href="mailto:Stephan.neuhaus@awi.de">Stephan.neuhaus@awi.de</a>, Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research&nbsp;</p> <p>Allan Cembella, <a href="mailto:Allan.Cembella@awi.de"> Allan.Cembella@awi.de</a>, Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research&nbsp;</p> <p>Lars Harms, <a href="mailto:Lars.Harms@awi.de"> Lars.Harms@awi.de</a>, Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, and Helmholtz Institute for Functional Marine Biodiversity</p> <p>Uwe John, <a href="mailto:Uwe.John@awi.de"> Uwe.John@awi.de</a>, Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, and Helmholtz Institute for Functional Marine Biodiversity</p> <p>JOURNAL:</p> <p>Frontiers in Marine Science, section Marine Molecular Biology and Ecology</p> <p>MS-ID:</p> <p>521330</p> <p>HOWTO:</p> <p>Sequences identified as Alveloates or Stramenopiles (description in manuscript) had been were classified more accurately by PhyloAssigner version 6.166 (https://github.com/jungbluth/phyloassigner, Vergin et al., 2013, DOI:10.1038/ismej.2013.32) with a phylogenetic placement onto reference trees based on 18S/28S concatenated alignments, according to Elferink et al. 2017 (DOI: 10.1016/j.dsr.2016.11.002).</p> <p>CONTENT:</p> <p>reference databases:</p> <p>- Alveolata_SSU-LSU-concat_310715_636.phyloassignerdb</p> <p>- Stramenopiles_SSU_LSU_concat_030815_1777.phyloassignerdb</p> <p>query sequence files:</p> <p>- Alveolata_seqtab_SIGN_dada2.fasta</p> <p>- Alveolata_seqtab_SIGN_dada2.fasta</p> <p>created out put folder including the taxonomic annotation:</p> <p>- Alveolata_seqtab_SIGN_dada2.place.out</p> <p>- Stramenopiles_seqtab_SIGN_dada2.place.out</p> <p>text file containing the used commands:</p> <p>- commands</p> <p>&nbsp;</p> <p>&nbsp;</p> <p>&nbsp;</p> <p>&nbsp;</p>