Catalysis of Ac-DEVD-AMC by procaspase-3 (dataset formatted for analysis by interferENZY)

<p><strong>Main description</strong></p> <p>This dataset depicts the catalysis of the fluorogenic substrate Ac-DEVD-AMC to the fluorescent substrate AMC by recombinant procaspase-3 obtained in yeast cell extracts, for fixed concentration of enzyme and variation of concentration of initial substrate. It was originally documented&nbsp;in&nbsp;<em>Biophysical Chemistry 252 (2019) 106193</em> (<a href="https://doi.org/10.1016/j.bpc.2019.106193">https://doi.org/10.1016/j.bpc.2019.106193</a>), and then used as a study case for the&nbsp;webserver interferENZY (a web-based tool for enzymatic assay validation and standardized kinetic analysis;&nbsp;visit <a href="https://interferenzy.i3s.up.pt">https://interferenzy.i3s.up.pt</a> for more information). To this end, it was converted to the format here presented:&nbsp;tab-separated *.txt input required for interferENZY analysis.</p> <p>&nbsp;</p> <p><strong>Dataset organization</strong></p> <p>Line 1: Tab-separated initial concentrations of substrate Ac-DEVD-AMC in micromolar (&micro;M) concentration</p> <p>Line 2: Concentration of protein in yeast extract (0.123 mg/mL)</p> <p>Line 3: Units of time</p> <p>Line 4: Units of concentration for substrate values and&nbsp;measurements</p> <p>Line 5: Dataset name</p> <p>Line 6 and downwards: Tab-separated column-pairs of the progress curves (time,Product)&nbsp;corresponding to the indicated values of initial concentrations of substrate in line 1</p> <p>&nbsp;</p> <p><strong>Contact information:</strong></p> <p>Maria Filipa Pinto (mfpinto@i3s.up.pt)<br> Pedro M. Martins (pmartins@ibmc.up.pt)</p> <p>i3S &ndash; Instituto de Investiga&ccedil;&atilde;o e Inova&ccedil;&atilde;o em Sa&uacute;de, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal. Telephone number: +351 226 074 900</p>