Fig. 2 in Analyses of Soft Tissue from Tyrannosaurus rex Suggest the Presence of Protein

Fig. 2. In situ immunochemistry on 300-nm sections of demineralized MOR 1125 cortical bone (A to D) and medullary bone (E to H). (A) and (E), no primary antibodies added (negative control); (B) and (F), antibodies to avian collagen I; (C) and (G), anti­ bodies to actin protein (nonrelevant, negative control); (D) and (H), anti­ bodies to avian collagen I, inhibited by incubating with purified chicken colla­ gen before exposing to dinosaur tissues. All data were collected using the same parameters at 122-ms inte­ gration. (I to K) MOR 1125 cortical tissue exposed to (I) no primary, (]) antibodies to avian collagen I, or (K) collagenase digestion followed by antibodies to avian collagen I, as described {11). Data in (I), (J), and (K) were collected at 149-ms integration.