Twist - torsion coupling in beating axonemes

<p>The dataset published here was used to measure a<strong> high resolution 3D wavefom </strong>of isolated and <strong>reactivated axonemes from <em>Chlamydomonas reinhardtii</em></strong>.</p> <p><a href="https://doi.org/10.1101/2024.03.18.585533"><span><span>doi:</span> https://doi.org/10.1101/2024.03.18.585533 </span></a></p> <p>It was further used to show <strong>twist-torsion coupling </strong>in these axonemes.</p> <p>The data is organized in six folders:</p> <p><strong>1) high resoluton 3D averaged waveform of isolated and reactivated axonemes from&nbsp;<em>Chlamydomonas Reinhardtii</em>.</strong><br>Data files (MATLAB and txt format) contain the 3D coordinates (along the 3D arc-length) of 32 axonemal shapes that comprise one beat cycle. <br>A corresponding txt file describes the details of the dataset.&nbsp;</p> <p><strong>2) 3D waveforms of single isolated and reactivated axonemes from&nbsp;<em>Chlamydomonas Reinhardtii</em>.</strong><br>Data files (MATLAB and txt format) contain the 3D shapes of 17 individual axonemes obtained from defocused darkfield-microsopy images. <br>A corresponding txt file describes the details of the dataset.</p> <p><strong>3) Image Raw Data of single isolated and reactivated axonemes used to reconstruct the 3D waveform<br></strong>Movie files (multi-layer tif) of reactivated axonemes imaged with defocused-darkfield-microscopy. <br>A corresponding txt file describes the details of the dataset.<strong><br></strong></p> <p><strong>4) Calibration of defocused darkfield-microscopy.&nbsp;<br></strong>Data file (MATLAB) contains the relationship between the z-position relative to the focal plane and the full-width-at-half-maximum (FWHM) of the axoneme signal, measured normal to the centerline as well as the z-stack of imges (multi-layer tif) used to extract this relation. <br>A corresponding txt file describes the details of the dataset.</p> <p><strong>5)</strong> <strong>Distance between gold nano paricle (GNP) and the axonemal centerline as a function of the beat cycle</strong><br>Data file (MATLAB) contains 20 measurements of d_C (where d_C is the normal distance between the center position of the GNP and the axoneme centerline in 2D images) as a function of time. A corresponding txt file describes the details of the dataset.</p> <p><strong>6) Image Raw Data of single isolated and reactivated axonemes with attached GNPs used to measure d_C.&nbsp;<br></strong>Movie files (multi-layer tif) of reactivated axonemes with attached gold nano particles (GNPs) imaged with darkfield-microscopy. <br>A corresponding txt file describes the details of the dataset.</p>