Fig. 4 in Mitophylogeny of Pangasiid Catfishes and its Taxonomic Implications for Pangasiidae and the Suborder Siluroidei.

Fig. 4. Detailed PhyML-phylogeny based on the analysis of the partial cytB sequences (634 bp) showing the detailed relationships of the family Pangasiidae and related families (Austroglanididae, Ictaluridae, and Cranoglanididae). In total, 80 sequences, including 78 from Pangasius and Pangasianodon and 2 outgroup sequences from the order Clupeiformes, were included (Table S3). The alignment was performed by MAFFT (Katoh and Standley 2013), curated by BMGE v1.12 (Criscuolo and Gribaldo 2010), the tree was reconstructed in PhyML 3.3 (Guindon et al. 2010) using a maximum likelihood method and 1000 bootstrap resamplings, and the output Newick tree was extracted and visualized using FigTree v1.4.4 (Rambaut 2018). The basal nodes of the Pangasiidae and two sister groups (Pangasianodon and (Pangasius + Helicophagus + Pseudolais)) are shown by arrows. The Pangasius mekongensis, Pangasianodon hypophthalmus, and Pangasius krempfi sequences in this study are bolded. The taxonmisidentified sequences were added with a question mark at the end. The taxa from Pangasiidae were shortened and those from other related families were presented with their full names. The abbreviations of the isolates are given in brackets, including the geographical origin or voucher records of each sequenced specimen (where available), which were retrieved from the previous studies. The country of origin or where the sample was reported is given in full or in brackets, if available. Accession numbers are given at the end of each sequence label. The scale bar represents the number of substitutions per site.