Coexpression and purification of HTT and HTT-HAP40 with NEDD4L and HAP1 2019/05/02

<p><strong>Project:&nbsp;</strong>Biophysical investigation of purified HTT protein and protein complex samples</p> <p><strong>Experiment:&nbsp;</strong>Coexpression and purification of HTT and HTT-HAP40 with NEDD4L and HAP1</p> <p><strong>Date completed:&shy;&nbsp;</strong>2019/05/02</p> <p><strong>Rationale:&nbsp;</strong>HAP1 and NEDD4L have been suggested as high probability interaction partners of HTT although it is unclear if they interact with HTT or HTT-HAP40. Coexpression of the constructs was conducted in baculoviral expression system (Sf9 cells) to determine if they copurify with HTT or HTT-HAP40.&nbsp;</p>