Huntingtin-RNA complex generation for cryoEM analysis 2019/07/24

<p><strong>Project:&nbsp;</strong>High resolution structural analysis of HTT-nucleic acid complexes</p> <p><strong>Experiment:&nbsp;</strong>Huntingtin-RNA complex generation for cryoEM analysis</p> <p><strong>Date:&nbsp;</strong>2019/07/24</p> <p><strong>Background:&nbsp;</strong>Huntingtin has been shown to copurify with nucleic acid material as well as bind different nucleic acid species in band shift assays. To more thoroughly characterise this interaction, HTT-nucleic acid complex generation and structure solution should provide insight into how huntingtin performs this function.&nbsp;</p> <p><strong>Rationale:&nbsp;</strong>Previous attempts to capture HTT-nucleic complexes have been difficult and maintaining the complex throughout purification of the sample has been tricky. These four approaches are different ways to try and form a stable complex between Q54 HTT-HAP40 and CAG₈RNA.&nbsp;</p>