Cdc42 couples T cell receptor endocytosis to GRAF1-mediated tubular invaginations of the plasma membrane

<p>This Dataset contains primary data used for the publication &quot;Cdc42 couples T cell receptor endocytosis to GRAF1-mediated tubular invaginations of the plasma membrane&quot; published online on 04. November 2019<br> doi:10.3390/cells8111388</p> <p><strong>Abstract:</strong> T cell activation is immediately followed by internalization of the T cell receptor (TCR).<br> TCR endocytosis is required for T cell activation, but the mechanisms supporting removal of TCR<br> from the cell surface remain incompletely understood. Here we report that TCR endocytosis is<br> linked to the clathrin-independent carrier (CLIC) and GPI-enriched endocytic compartments<br> (GEEC) endocytic pathway. We show that unlike the canonical clathrin cargo transferrin or the<br> adaptor protein Lat, internalized TCR accumulates in tubules shaped by the small GTPase Cdc42<br> and the Bin/amphiphysin/Rvs (BAR) domain containing protein GRAF1 in T cells. Preventing<br> GRAF1-positive tubules to mature into endocytic vesicles by expressing a constitutively active<br> Cdc42 impairs the endocytosis of TCR, while having no consequence on the uptake of transferrin.<br> Together, our data reveal a link between TCR internalization and the CLIC/GEEC endocytic route<br> supported by Cdc42 and GRAF1.</p> <p>&nbsp;</p> <p>Data are organised in compressed (.zip) folders entitled as the corresponding Figures in the publication.</p> <p>Programs we recommend to view the files are:<br> .fcs files:&nbsp;&nbsp;&nbsp;&nbsp; FlowJo software v10 (Tree Star, Ashland, OR, USA)<br> .lif files:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; LAS X v3 (Leica Microsystems, Wetzlar, Germany)<br> .pzfx files:&nbsp;&nbsp; Prism v7 software (GraphPad, San Diego, CA, USA)</p> <p>&nbsp;</p> <p>In case this Dataset is updated, new version will be available with doi:10.5281/zenodo.3545842</p>