Dataset for the article "Electron-Transfer-Mediated by Detonation Nanodiamonds for Enhanced Cortisol Immunoassay"

<p>Dataset for the article "Electron-Transfer-Mediated by Detonation Nanodiamonds for Enhanced Cortisol Immunoassay".</p> <p>Chakavak Esmaeili1*, Jaroslav Kuliček1, Mark&eacute;ta &Scaron;lapal Bařinkov&aacute;1, Bohuslav Rezek1*</p> <p>1 Faculty of Electrical Engineering, CTU in Prague, Technicka 2, 16627 Prague, Czech Republic</p> <p>Figure 1. The preparation and immobilization step of the cortisol fabrication based on electrochemical immunosensor&nbsp;<br>Figure 2. 3D AFM images &nbsp;of (1) carbon bare electrode, (2) carbon electrode /DND, (3) carbon electrode/DND/EDC NHS (4) carbon electrode/ DND /EDC NHS/Ab, (5) carbon electrode/ DND /EDC NHS/Ab /BSA, (6) carbon electrode/ DND /EDC NHS/Ab/BSA/Ag<br>Figure 3. Raman spectra of immunosensor composition<br>Figure 4. &nbsp;FTIR spectra of DND (gray line), DND/ EDC NHS (blue line), DND/EDC NHS/Ab (green line). All spectra were recorded in the region between 4000&ndash;800 cm&minus;1 with a resolution of 4 cm&minus; 1 and 128 scans<br>Figure 5. Functionalization step. (1) carbon bare electrode, (2) carbon electrode /DND, (3) carbon electrode /DND/EDC NHS, (4) carbon electrode/DND/ EDC NHS/Ab and sensing step (5) carbon electrode/DND/ EDC NHS/Ab/BSA and (6) carbon electrode/DND/ EDC NHS/Ab/BSA/Ag in the presence of 100 nM cortisol in pH 7.0, containing 250 mM KCl and 5 mM K3[Fe(CN)6] and 5 mM K4[Fe(CN)6], Scan rate 0.1Vs&minus;1<br>Figure 6. (a) Calibration curve of DPV with varying Ag concentrations. (b) Sensitivity comparison between configurations without Ab receptor (carbon electrode/DND/EDC NHS/Ag) and with Ab receptor (carbon electrode/DND/EDC NHS/Ab/BSA/Ag) in PBS solution, pH 7.0, containing 250 mM KCl and 5 mM K3[Fe (CN)6] and 5 mM K4[Fe (CN)6]<br>Figure 7. Interference study involving progesterone (2430 pg/ml), &beta;-oestradiol (32 pg/ml), cortisone (28.6 nM), and corticosterone (3.94 nM) with respect to cortisol (100nM)<br>Figure 8. Stability of the immunosensor with repeated measurements of cortisol</p> <p>&nbsp;</p> <p>Figure S1. &nbsp;Optical images obtained for (1) bare carbon electrode, (2) DND, (3) DND/EDC NHS, (4) DND/EDC NHS/Ab, (5) DND/EDC NHS/Ab/BSA, (6) DND/EDC NHS/Ab/BSA/Ag.<br>Figure S2. SEM images of (1) bare carbon SPE, (2) carbon electrode/ DND, (3) carbon electrode/ DND /EDC NHS, (4) carbon electrode/ DND /EDC NHS/Ab, (5) carbon electrode/DND/EDC NHS/Ab/BSA, (6) carbon electrode/ DND /EDC NHS/Ab/BSA/Ag<br>Figure S3. (a) CV and (b) DPV of carbon bare electrode, carbon electrode /EDC NHS, carbon electrode/EDC NHS/Ab, carbon electrode/EDC NHS/Ab/BSA, carbon electrode/EDC NHS/Ab/BSA/Ag, in the presence of 100 nM cortisol, &nbsp;pH 7.0, containing 250 mM KCl and 5 mM K3[Fe(CN)6] and 5 mM K4[Fe(CN)6], Scan rate 0.1Vs&minus;1.</p> <p><br>Table 1. Determination of cortisol in artificial saliva sample (n = 3)<br>Table S1. Surface roughness of immunosensors</p>