Fig. 8 in Description of two new species forming a sympatric species pair of Crenicichla (Teleostei: Cichlidae) endemic to the Piray Guazú River in the Paraná River Basin, Misiones, Argentina and belonging to the C. mandelburgeri species complex

Fig. 8. Overview of phylogenetic relationships and molecular and morphological differentiation of C. ama sp. nov. and C. aravera sp. nov. and its parallel species pair (C. ypo Casciotta, Almirón, Piálek, Gómez & Říčan, 2010 and C. yjhui Piálek, Casciotta, Almirón & Říčan, 2019). A. Summary of phylogenetic relationships within the C. mandelburgeri species complex with a nDNA ddRAD SVDQ species tree analysis as main topology focusing on the parallel species pairs in the Piray Guazú (the two new species) and Urugua-í River basins. Species tree is based on 60% SNP-representation matrix using fixed (= homozygotic sites). Red and blue basal branches and nodes in the species tree topology show biogeography (Red: Iguazú, Blue: Middle Paraná). Numbers in parentheses are total numbers of analyzed individuals (in nDNA/ in mtDNA). Numbers at nodes show bootstrap support values. Species colours as in Fig. 5. Blue arrows show nDNA introgression from C. mandelburgeri into C. aravera and C. yjhui. Modified from Říčan et al. (2021b). B. Alternative topology to the right of the tree represents mtDNA relationships of species in the two focal species pairs. Only C. ypo does not have a mitonuclear phylogenetic conflict. Modified from Říčan et al. (2021b). C. Inference of population structure based on the Admixture analysis for the two focal species pairs. Note majority assignment of C. yjhui and C. aravera with C. ypo and C. ama and partial assignment to C. mandelburgeri sensu lato at K10 to K13, and complete separation of all species at K14. Modified from Říčan et al. (2021b). D. Morphometric variation and discrimination of the two species pairs of sympatric species from the Piray Guazú (C. ama, C. aravera) and Urugua-í (C. ypo, C. yjhui) River basins analyzed by PCA based on holotype and paratype specimens ≥ 70 mm SL. Morphological measurements followed Kullander's (1986) methodology for Crenicichla Heckel, 1840 and were used as proportional values in % of SL. Note complete separation of species between ecomorphs (sympatric) and partial separation within ecomorphs (allopatric). Modified from Říčan et al. (2021b).