Structure of human TRPM8 channel

<p>This repository includes the following source datasets:</p> <p><strong>1. modeling.zip</strong><br> Modeling of icilin binding to HsTRPM8 (Figure 3e, 3f, 3g, 3h)<br> &nbsp;- HsTRPM8-KX7_opt.pdb: original pose (Figure 3e, 3f)<br> &nbsp;- HsTRPM8-KX7_opt_rotated.pdb: rotates pose (Figure 3g, 3h)</p> <p><strong>2. activation_assay.zip</strong><br> HsTRPM8 activation assay results (Figure 3i and Supplementary Figure 5)<br> &nbsp;- HsTRPM8_activation_assay_results.pzf: GraphPad file with activation Assay results</p> <p><strong>3. Fr-TM.zip</strong><br> Structure-based multiple sequence alignment of TRPM channels (Supplementary Figure 4a)<br> &nbsp;- Fr-TM_info.txt: description of procedure used to create alignment<br> &nbsp;- TRPM_new_raw_pairwise_alignments.fas: raw pairwise alignment of new TRPM sequences to 6CO7 reference<br> &nbsp;- TRPM_S6_restriction_site_aligned.fas: final multiple sequence alignment used to generate Supplementary Figure 4a</p> <p><strong>4. 3Dvar.zip</strong><br> HsTRPM8 3D Variability Analysis results (Supplementary Movie 1)<br> &nbsp;- *.mrc files: composite maps with extreme frames for two analyzed components (aligned with focused MHR1/2 map)<br> &nbsp;- *.pdb files: HsTRPM8 models fitted (MDFF) to above maps and used to generate Supplementary Movie 1</p> <p><strong>5. Supplementary_Movie_1.mp4</strong><br> Morphing between different conformations of HsTRPM8 resolved by 3D Variability analysis (Supplementary Movie 1).</p>