rCRUX Generated MarVer3 Marine Mammal Reference Database

<p>rCRUX generated reference database&nbsp;using NCBI nt blast database downloaded in December 2022.</p> <p>Primer Name:&nbsp; MarVer3 Marine Mammal<br> Gene:&nbsp; &nbsp;16S<br> Length of Target:&nbsp; &nbsp; 232-274<br> get_seeds_local() minimum length:&nbsp; &nbsp; 160<br> get_seeds_local() maximum length:&nbsp; &nbsp; 345<br> blast_seeds() minimum length:&nbsp; &nbsp; 124<br> blast_seeds() maximum length:&nbsp; &nbsp; 309<br> max_to_blast:&nbsp;&nbsp;100<br> Forward Sequence (5&#39;-3&#39;):&nbsp; &nbsp;AGACGAGAAGACCCTRTG<br> Reverse Sequence (5&#39;-3&#39;):&nbsp; &nbsp; GGATTGCGCTGTTATCCC<br> Reference:&nbsp; &nbsp; Valsecchi, E., Bylemans, J., Goodman, S. J., Lombardi, R., Carr, I., Castellano, L., ... &amp; Galli, P. (2020). Novel universal primers for metabarcoding environmental DNA surveys of marine mammals and other marine vertebrates. Environmental DNA, 2(4), 460-476.&nbsp;https://doi.org/10.1002/edn3.72</p> <p>We chose default rCRUX parameters for&nbsp;<em>get_blast_seeds</em>() of percent coverage of 70, percent identity of 70, evalue 3e+7, and max number of blast alignments = &#39;100000000&#39; and for&nbsp;<em>blast_seeds</em>() of coverage of 70, percent identity of 70, evalue 3e+7, rank of genus, and max number of blast alignments = &#39;10000000&#39;. &nbsp;</p>