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FIGURE 2 in Description of coccoid cyanoprokaryote Nisada stipitata morphogen. et sp. nov. from the supralittoral zone in the tropical Mexican Pacific

FIGURE 2. Morphology of Nisada stipitata. A. Macroscopic view of partially covered rock (black portion). B. Macroscopic view of strip of biofilm on rock. C. (tv) Pseudofilament apices in fluorescent microscopy. D. (tv) Edge of biofilm with pseudofilaments in a row. E. (tv) Apices of morph 2 before cell division (single arrow) and after division but before separation (double arrow). F. With dying filaments of Kyrthuthrix cf. maculans. G. (tv) Colonial mucilage (single arrow); apices of morphs 1 and 2 and (lv) two morphs 2 (double arrows). H. (tv) Apices of morph 1 with a few pseudofilaments in lv (arrow heads); pad of a complete pseudofilament (white arrow) and colonial mucilage (black arrow). I. (lv) Pseudofilament of morph1 with developed stipe and cups; "horns" (sheath) with synchronous growth (arrows). Daughter much larger than mother cell.J. (lv) Pseudofilament of morph 1 without pad (base of stipe, arrow). K. (lv) Pseudofilament of morph 1 showing "horns" (sheath) with asynchronous growth (arrows). L, M. Diversity of morphology of morph 1. Micrographs D–M with light microscopy. tv = top view, lv = lateral view. Scale bars: A = 1 mm, B = 200 μm, C = 25 μm, D–H, J, L = 6 μm I, K, M = 3 μm.

FIGURE 1 in Description of coccoid cyanoprokaryote Nisada stipitata morphogen. et sp. nov. from the supralittoral zone in the tropical Mexican Pacific

FIGURE 1. San Agustín Bay, state of Oaxaca, México. A. Map showing location of collection site. B. Panoramic photograph of the supralittoral zone of the site.

FIGURE 4 in Description of coccoid cyanoprokaryote Nisada stipitata morphogen. et sp. nov. from the supralittoral zone in the tropical Mexican Pacific

FIGURE 4. (lv) Line drawings of Nisada stipitata. A. Edge of biofilm with pseudofilaments in a row. B. Complete pseudofilament of morph 1. C. Complete pseudofilament of morph 2. Ps = pseudofilament sheath, dc = daughter cell, c = cup, mc = mother cell, st = stipe,

FIGURE 3 in Description of coccoid cyanoprokaryote Nisada stipitata morphogen. et sp. nov. from the supralittoral zone in the tropical Mexican Pacific

FIGURE 3. Stages of reproduction of Nisada stipitata in lateral view. A. (lv) Two pseudofilaments of morph 1 with and without cell division; perpendicular plane of division evident in left pseudofilament. B. Two-celled pseudofilament of morph 1 with beginning of perpendicular cell division of the daughter cell (top arrow); displaced basal/mother cell allowing partial view of the inside of the cup (bottom arrow). C. (lv) Two pseudofilaments of morph 1. Three-celled pseudofilament with fracture (arrow). D. (lv) Complete pseudofilament of morph 2 with division of daughter cell. E, F. Diversity of stages of the life cycle of morph 1. Pseudofilaments with all or some of the differentiated mucilaginous structures. Basal cell with incipient cup (arrow). G. (lv) Empty cups/stipes set in the colonial mucilage (arrows). H. (lv) Basal cells with different degrees of development, set in colonial mucilage. Scale bars: A–E = 3 μm, F–H = 6 μm.

Table 6 in Taxonomy and molecular phylogenetic position of new species and new records of Coelosphaeridae (Demospongiae: Poecilosclerida) from the Mexican Pacific

Table 6. Distribution of polymorphic sites in the 28S DNAr region (C1–D2 domain) for four specimens of L. (W.) hawaiiana, one from Hawaii (Vicente et al. 2020) and three from the Mexican Pacific (this study)<table><tbody><tr><th>L. (W.) hawaiiana</th><th></th><th></th><th>Positions of mutations</th></tr></tbody><tbody><tr><th>Specimens</th><td>Locality</td><td>GenBank accession number</td><td>566</td><td>608</td><td>633</td></tr><tr><th>LEB-ICML-UNAM-2441</th><td>Playa la Concha, Baja California Sur</td><td>OP704020</td><td>T</td><td>-</td><td>T</td></tr><tr><th>LEB-ICML-UNAM-2447</th><td>Playa la Concha, Baja California Sur</td><td>OP704019</td><td>T</td><td>-</td><td>T</td></tr><tr><th>LEB-ICML-UNAM-3055</th><td>La Entrega, Oaxaca</td><td>OP704021</td><td>T</td><td>-</td><td>T</td></tr><tr><th>UF 3804</th><td>Hawaii</td><td>MT452537</td><td>C</td><td>G</td><td>-</td></tr></tbody></table>

Table 1 in Taxonomy and molecular phylogenetic position of new species and new records of Coelosphaeridae (Demospongiae: Poecilosclerida) from the Mexican Pacific

Table 1. List of references and GenBank accession numbers of sequences used in molecular analysis. The species names are referred to through the accession number in the tree topologies. Sequences generated in the present study are in bold<table><tbody><tr><th>Reference</th><th>GenBank accession number</th><th></th></tr><tr><th>28S</th><th>COI</th></tr></tbody><tbody><tr><th>Belinky et al. 2012</th><td></td><td>HE591461</td></tr><tr><th>Erpenbeck et al. 2007</th><td></td><td>EF519638, EF519639, EF519643, EF519640, EF519641</td></tr><tr><th>Hestetun et al. 2016</th><td>LN870644</td><td></td></tr><tr><th>Idan et al. 2018</th><td></td><td>KX866766</td></tr><tr><th>Medina et al. 2001</th><td>AY026376</td><td></td></tr><tr><th>Morrow et al. 2012</th><td>Hº379226, Hº379228, Hº379229, Hº379230, Hº379231</td><td>JF440339</td></tr><tr><th>Morrow et al. 2019</th><td>KF017189</td><td>KF017195</td></tr><tr><th>Nichols 2005</th><td>AY561883</td><td></td></tr><tr><th>Núñez et al. 2017</th><td></td><td>KY565309</td></tr><tr><th>Pérez et al. 2006</th><td>Dº241773</td><td></td></tr><tr><th>Regueiras et al. 2019</th><td></td><td>KY492550, KY492536, KY492520</td></tr><tr><th>Riesgo et al. 2013</th><td></td><td>JX999092, JX999090</td></tr><tr><th>Rossi et al. 2019</th><td></td><td>MK803199</td></tr><tr><th>Thacker et al. 2013</th><td>KC869522, KC869546, KC869556, KC869564, KC869447, KC869468, KC869537, KC869541, KC869613, KC869624, KC869629, KC869449, KC869501, KC869603, KC869597, KC869478, KC869489, KC869512, KC869608, KC869561, KC869479, KC869529, KC869506, KC869649, KC869548, KC869515, KC869627, KC869509</td><td></td></tr><tr><th>Vargas et al. 2012</th><td></td><td>HE611618, HE611608</td></tr><tr><th>Vargas et al. 2015</th><td></td><td>LN850204, LN850229, LN850210, LN850247, LN850251, LN850248</td></tr><tr><th>Vicente et al. 2020</th><td>MT452537</td><td></td></tr><tr><th>Wulff 2006</th><td></td><td>Dº133901, Dº133897</td></tr><tr><th>Present study</th><td>OP740700, OP740709, OP740710, OP704019, OP704020, OP704021</td><td>OP715763</td></tr><tr><th>GenBank direct submission</th><td></td><td>KJ620400, LT160711, AJ843890, LR655634, LR655638</td></tr></tbody></table>

Table 2 in Taxonomy and molecular phylogenetic position of new species and new records of Coelosphaeridae (Demospongiae: Poecilosclerida) from the Mexican Pacific

Table 2. Comparative data for the dimensions of the spicules (in µm) of the genus Celtodoryx, shass length × width; head diameter are given for megascleres and total length for arcuate isochelae and oxychaetes.Values in parentheses are means<table><tbody><tr><th>Celtodoryx species</th><th>Diactinal megascleres tylotes and strongyles (and derivates)</th><th>Arcuate isochelae</th><th>Oxychaetes</th></tr><tr><th>Celtodoryx chichiltik sp. nov.</th></tr><tr><th>Holotype</th></tr></tbody><tbody><tr><th>LEB-ICML-UNAM-144</th><td>TYa: 195–(213.8)–225 × 2–(2.65)–3; 2.5–(3.8)–5</td><td>20–(28)–32.5</td><td>51–(65.5)–95</td></tr><tr><td>STb: 213–(243.5)–273 × 2.5–(4.3)–7.5</td><td></td><td></td></tr><tr><th>Paratypes</th></tr><tr><th>LEB-ICML-UNAM-161</th><td>TY: 197.5–(231.7)–261.5 × 3–(4.2)–5; 4.5–(5.4)–6.3</td><td>25–(28)–30</td><td>55–(71.3)–100</td></tr><tr><td>ST: 215–(264)–287.5 × 5.5–(7.1)–8</td><td></td><td></td></tr><tr><th>LEB-ICML-UNAM-172</th><td>TY: 180–(206)–237.5 × 2.5–(3)–4.5; 3–(4)–5</td><td>20–(25.8)–30</td><td>30–(62.8)–90</td></tr><tr><td>ST: 187.5–(226)–287.5 × 3.7–(5.5)–8</td><td></td><td></td></tr><tr><th>LEB-ICML-UNAM-260</th><td>TY: 150–(238.5)–275 × 2.5–(4.8)–5; 3.8–(5)–7.5</td><td>22.5–(24.3)–27.5</td><td>47.5–(64.8)–78</td></tr><tr><td>ST: 235–(248.2)–287.5 × 5–(6.4)–7.5</td><td></td><td></td></tr><tr><th>LEB-ICML-UNAM-292</th><td>TY:190–(221.6)–250 × 2.5–(2.9)–5; 3.7–(4.9)–7</td><td>20–(23.6)–25</td><td>40–(67)–80</td></tr><tr><td>ST: 220–(230)–252.5 × 5–(6.4)–7.5</td><td></td><td></td></tr><tr><th>LEB-ICML-UNAM-618</th><td>TY:210–(225)245 × 2.5–(3.3)–5; 3–(4.8)–6.3</td><td>22.5–(24.2)–7</td><td>57.5–(69)–77.5</td></tr><tr><td>ST: 212.5–(228.3)–250 × 3–(3–8)–5</td><td></td><td></td></tr><tr><th>LEB-ICML-UNAM-740</th><td>TY: 187.5–(216)–237.5 × 2.5(3.4)–4.5; 1.3–(4)–6.3</td><td>21.3–(25.2)–27.5</td><td>30–(75.5)–90</td></tr><tr><td>ST: 207–(231)–270 × 5(5.5)–7</td><td></td><td></td></tr><tr><th>LEB-ICML-UNAM-797</th><td>TY: 180–(213)–240 × 2.5–(2.8)–3.8; 3–(3.5)–5</td><td>20–(21.5)–25</td><td>45–(65.5)–77.5</td></tr><tr><td>ST: 232.5–(248.3)–265 × 2–(4.2)–5.5</td><td></td><td></td></tr><tr><th>LEB-ICML-UNAM-850</th><td>TY: 180–(237.8)–257.5 × 2–(3.7)–4.5; 3–(4.8)–7.5</td><td>20–(22.5)–25</td><td>62–(71)–75</td></tr><tr><td>ST: 207.5–(251)–297.5 × 2.5–(3.8)–7.5</td><td></td><td></td></tr><tr><th>Celtodoryx ciocalyptoides (Burton, 1935) for comparison</th></tr><tr><th>Lectotype ZIN 10844e</th><td>SETYc: 170–(208)–290 × 1.5–(4.5)–6.5; 1.5–(5.7)–7.2</td><td>I: 48–(51)–56</td><td>62–(67)–72</td></tr><tr><td>AnSTd: 190–(273)–305 × 3.2–(8)–10.4</td><td>II: 22–(25)–30</td><td></td></tr><tr><th>Paratype (MNHN D JV 93) of Celtodoryx girardae e</th><td>AnST: 165–(268)–320 × 5.8–(8.9)–12</td><td>I: 40–(48)–54</td><td>65–(74)–87</td></tr><tr><td>SETY:: 125–(195)–220 × 1.6–(4.2)–5.6, 2.4–(4.3)–6.4</td><td>II: 19–(23)–27</td><td></td></tr></tbody></table>aTY,Smooth tylotes, bST, Smooth strongyles, cSETY,spined ends tylotes, dAnST,spined ends anisostrongyles, eSpicule measures from Henkel and Janussen 2011.

Table 4 in Taxonomy and molecular phylogenetic position of new species and new records of Coelosphaeridae (Demospongiae: Poecilosclerida) from the Mexican Pacific

Table 4. Comparative data for the dimensions of the spicules (in µm) of L. (L.) albemarlensis and L. (L.) isodictyalis. Shass length × width; head diameter are given for megascleres and total length for arcuate isochelae and sigmas. Values in parentheses are means<table><tbody><tr><th>L. (L.) albemarlensis material examined</th><th>Styles</th><th>Tylotes</th><th>Arcuate isochelae</th><th>Sigmas</th></tr></tbody><tbody><tr><th>AHF-1736-49</th><td>150–(161.5)–175 × 2.5–(3.4)–5</td><td>165–(175.1)–185 × 2.5–(2.5)–5; 4.5–(5.5)–7</td><td>17.5–(24.8)–30</td><td>15–(26.3)–37.5</td></tr><tr><th>AHF-608-36</th><td>160–(169.2)–180 × 2.5–(3.5)–5</td><td>180–(193.3)–205 × 2.5–(3.1)–5</td><td>17.5–(21.8)–27.5</td><td>15–(18.5)–25</td></tr><tr><th>LEB-ICML-UNAM-27</th><td>150–(169.5)–185 × 2.5–(4.5)–7.5</td><td>125–(178.6)–200 × 2.5–(3)–5; 3.8–(5)–6.3</td><td>11.3–(20.5)–32</td><td>15–(28.2)–40</td></tr><tr><th>LEB-ICML-UNAM-183</th><td>142.5–(159.5)–180 × 2.5–(4.2)–6.3</td><td>155–(170.3)–205 × 2.5–(4)–6.3; 5–(5.9)–7.5</td><td>20–(27)–30</td><td>20–(26.6)–31.3</td></tr><tr><th>LEB-ICML-UNAM-190</th><td>150–(166)–187.5 × 2.5–(4.9)–8.8</td><td>153.8–(172.5)–185 × 2.5–(3.6)–5; 3.8–(6)–8.8</td><td>12.5–(18.3)–27.5</td><td>17.5–(25.9)–30</td></tr><tr><th>LEB-ICML-UNAM-191</th><td>142.5–(172.2)–185 × 1.3–(5.2)–8.8</td><td>162–(178.8)–195 × 2.5–(3.5)–5; 5–(5.9)–7</td><td>12.5–(17.2)–27.5</td><td>15–(26)–31.3</td></tr><tr><th>LEB-ICML-UNAM-193</th><td>135–(157.5)–185 × 1.3–(4.3)–6.3</td><td>150–(174)–190 × 2.5–(3.9)–5; 5–(5.7)–6.3</td><td>22.5–(25.5)–30</td><td>20–(26.3)–35</td></tr><tr><th>LEB-ICML-UNAM-232</th><td>170–(183.4)–195 × 2.5–(5.9)–10</td><td>127.5–(172.8)–190 × 2.5–(3.6)–5</td><td>10–(19.4)–30</td><td>15–(25.1)–40</td></tr><tr><th>LEB-ICML-UNAM-720</th><td>165–(177.2)–190 × 2.5–(3.7)–5</td><td>170–(181.6)–200 × 2.5–(3.3)–5</td><td>12.5–(21.3)–27.5</td><td>15–(27.0)–40</td></tr><tr><th>LEB-ICML-UNAM-1018</th><td>145–(160.2)–175 × 2.5–(2.5)–2.5</td><td>180–(195.8)–215 × 2.5–(2.9)–5</td><td>22.5–(25.9)–30</td><td>15–(23.4)–27.5</td></tr><tr><th>LEB-ICML-UNAM-1104</th><td>150–(157.9)–175 × 2.5–(3.8)–5</td><td>175–(187.9)–215 × 2.5–(2.9)–5</td><td>17.5–(23.3)–27.5</td><td>20–(26.1)–30</td></tr><tr><th>LEB-ICML-UNAM-1369</th><td>150–(162.8)–180 × 2.5–(2.8)–5</td><td>170–(186.5)–200 × 2.5–(2.6)–5</td><td>20–(25.7)–30</td><td>20–(23.3)–27.5</td></tr><tr><th>LEB-ICML-UNAM-1443</th><td>145–(161.6)–175 × 2.5–(3.0)–5</td><td>170–(181.2)–195 × 2.5–(2.7)–5</td><td>20–(23.4)–30</td><td>20–(25.1)–32.5</td></tr><tr><th>LEB-ICML-UNAM-1553</th><td>150–(160.4)–180 × 2.5–(2.7)–5</td><td>170–(180.9)–195 × 2.5–(2.7)–5</td><td>15–(24.1)–30</td><td>17.5–(23.5)–27.5</td></tr><tr><th>LEB-ICML-UNAM-1579</th><td>155–(170.2)–190 × 2.5–(3.6)–5</td><td>180–(186.5)–195 × 2.5–(2.7)–5</td><td>12.5–(19.8)–27.5</td><td>15–(25.5)–37.5</td></tr><tr><th>LEB-ICML-UNAM-1581</th><td>150–(169.2)–185 × 2.5–(3.2)–5</td><td>170–(181.2)–200 × 2.5–(2.7)–5</td><td>12.5–(21.9)–27.5</td><td>17.5–(28.1)–40</td></tr><tr><th>LEB-ICML-UNAM-2374</th><td>125–(150.3)–175 × 2–(4.5)–7.5</td><td>162.5–(171.3)–190 × 3–(5)–6; 5–(5.7)–7.5</td><td>15–(25.5)–32</td><td>15–(26)–30</td></tr><tr><th>LEB-ICML-UNAM-2377</th><td>167.5–(185)–198 × 2.5–(5.7)–7.5</td><td>162.5–(190)–200 × 2.5–(4.2)–5; 5–(5.5)–6.3</td><td>12.5–(22.2)–31.3</td><td>17.5–(33.6)–40</td></tr><tr><th>LEB-ICML-UNAM-2379</th><td>142.5–(158)–165 × 1.3–(6.2)–7.5</td><td>120–(167)–182.5 × 2.5–(4.3)–5; 3.8–(4.8)–5.5</td><td>17.5–(25.3)–32.5</td><td>20–(26.5)–32.5</td></tr><tr><th>For comparison (non-examined material)</th></tr><tr><th>L. (L.) albemarlensis from Desqueyroux-Faúndez and Van Soest 1997</th><td>123–(157)–190 × 4–8</td><td>107–(139)–170 × 5</td><td>16–26</td><td>16–26</td></tr><tr><th>L. (L.) isodictyalis (Carter, 1882); from Rützler et al. 2007</th><td>148.6–(164.6)–175.2 × 3.8–(4.8)–5.7</td><td>177.9–(198.3)–207.9 × 3.1–(3.9)–4.2</td><td>19.6–(26.3)–41.4</td><td>18.3(21)–22.9</td></tr></tbody></table>

Figure 7 in Tedania (Porifera: Demospongiae: Poecilosclerida) from the Mexican Pacific with the description of two new species

Figure 7. Drawings of the grouping of the Tedania and Trachytedania species bearing ectosomal diactinal spicules. Tedania species have ectosomal diactinal spicules, choanosomal monactinal spicules and onychaetes. Tedaniopsis species have ectosomal diactinal spicules, choanosomal diactinal spicules and onychaetes.

Figure 6 in Tedania (Porifera: Demospongiae: Poecilosclerida) from the Mexican Pacific with the description of two new species

Figure 6. Drawings of the subgeneric classification of the genus Tedania by Van Soest (2002): Tedania species have ectosomal diactinal spicules with microspined heads, choanosomal monactinal or diactinal spicules and onychaetes. Tedaniopsis species have ectosomal diactinal spicules with smooth heads, choanosomal monactinal and diactinal spicules and onychaetes. Trachytedania species have ectosomal diactinal spicules with smooth heads, choanosomal monactinal spicules with smooth or microspined base (styles or acanthostyles) and onychaetes.

Figure 5 in Tedania (Porifera: Demospongiae: Poecilosclerida) from the Mexican Pacific with the description of two new species

Figure 5. Tedania (Tedania) fulvum sp. nov. (a) Choanosomal styles. (b) Ectosomal tylotes with smooth heads. (c) Onychaetes in different sizes. (d) Closer view of the microspined surface of the onychaetes.

Figure 4 in Tedania (Porifera: Demospongiae: Poecilosclerida) from the Mexican Pacific with the description of two new species

Figure 4. Tedania (Tedania) tropicalis sp. nov. (a) Ectosomal tylotes with microspined heads. (b) Choanosomal styles. (c) Onychaetes.

Figure 1 in Tedania (Porifera: Demospongiae: Poecilosclerida) from the Mexican Pacific with the description of two new species

Figure 1. Drawings of the current classification of the family Tedaniidae Ridley and Dendy 1886 (van Soest et al. 2017). Hemitedania species have oxeas as ectosomal and choanosomal spicules and onychaetes; Strongylamma species have strongyles as ectosomal and choanosomal spicules and onychaetes; Trachytedania species have ectosomal diactinal spicules (smooth heads), choanosomal monactinal spicules (acanthostyles and styles) and onychaetes; Tedania (Tedania) species have ectosomal diactinal spicules (smooth or microspined heads), choanosomal monactinal and diactinal spicules and onychaetes; Tedania (Tedaniopsis) species have ectosomal diactinal spicules (smooth heads), choanosomal monactinal and diactinal spicules and onychaetes; Tedania (Stylotedania) species have ectosomal and choanosomal monactinal spicules and onychaetes.

Figure 3 in Tedania (Porifera: Demospongiae: Poecilosclerida) from the Mexican Pacific with the description of two new species

Figure 3. External morphologies of the two new species. (a) Tedania (Tedania) tropicalis sp. nov.; (b) Tedania (Tedania) fulvum sp. nov.

Figure 2 in Tedania (Porifera: Demospongiae: Poecilosclerida) from the Mexican Pacific with the description of two new species

Figure 2. Distribution and sample localities of Tedania species from the tropical Eastern Pacific coast. Numbers refer to different species as follows: (1) Tedania (Tedania) tropicalis sp. nov.; (2) Tedania (Tedania) fulvum sp. nov.

Figure 9 in Two new deep-sea species of Argestidae and Ameiridae (Copepoda: Harpacticoida) from the Eastern Mexican Pacific and Gulf of California, with proposal of a new genus of the family Argestidae

Figure 9. Argestigens celibis sp. n., male holotype. (a) left P1, anterior, with aberrant EXP3; (b) right P1 EXP3, anterior; (c) P2, anterior.

Figure 7 in Two new deep-sea species of Argestidae and Ameiridae (Copepoda: Harpacticoida) from the Eastern Mexican Pacific and Gulf of California, with proposal of a new genus of the family Argestidae

Figure 7. Argestigens celibis sp. n., male holotype. (a) habitus, dorsal; (b) urosome, ventral, P5- bearing somite omitted; (c) urosome, lateral.

Figure 5 in Two new deep-sea species of Argestidae and Ameiridae (Copepoda: Harpacticoida) from the Eastern Mexican Pacific and Gulf of California, with proposal of a new genus of the family Argestidae

Figure 5. Argestes analongises sp. n., female holotype. (a) P3, anterior; (b) P4, anterior; (c) P4 EXP3.

Figure 3 in Two new deep-sea species of Argestidae and Ameiridae (Copepoda: Harpacticoida) from the Eastern Mexican Pacific and Gulf of California, with proposal of a new genus of the family Argestidae

Figure 3. Argestes analongises sp. n., female holotype. (a) mandible; (b) maxillule; (c) maxilla; (d) maxilliped.

Figure 1 in Two new deep-sea species of Argestidae and Ameiridae (Copepoda: Harpacticoida) from the Eastern Mexican Pacific and Gulf of California, with proposal of a new genus of the family Argestidae

Figure 1. Argestes analongises sp. n., female holotype. (a) urosome, dorsal, P5-bearing somite omitted; (b) urosome, ventral, P5-bearing somite omitted.