Find research datasets worth reusing

Multiplex imaging of breast cancer lymph node metastases identifies prognostic single-cell populations independent of clinical classifiers

This repository contains the raw IMC data of ZTMA 26 as continuation of dataset 10.5281/zenodo.7494413. The zip files starting with ZTMA contain the raw IMC measurements (mcd and txt) of those parts of the TMA. The TMA measurements are split up into parts in order to avoid huge files. Additionally, this repository contains the metadata of the patients analyzed in this study, the panel information and the single-cell data that was extracted from the multiplexed images together with the associated metadata in SingleCellExperiment format for analysis in R. The analysis.zip folder contains files that were written out during the analysis according to the scripts in https://github.com/BodenmillerGroup/BC_LN_metastses. The single-cell and other data outputs from CellProfiler can be found in the cpout.zip file. The IF_whole_sections.zip file contains the IF images of the primary breast cancer sections (czi files) and the extracted single-cell data.

Multiplex imaging of breast cancer lymph node metastases identifies prognostic single-cell populations independent of clinical classifiers

This repository contains the raw IMC data of ZTMA 21 and 25 of the matched primary breast cancer and lymph node metastasis study presented in Fischer and Jackson et al., 2023. The code that was used to process and analyze this data can be found at https://github.com/BodenmillerGroup/BC_LN_metastses. The zip files starting with ZTMA contain the raw IMC measurements (mcd and txt) of the respective parts of the TMA. The TMA measurements are split up into parts in order to avoid huge files.

opencc-by-4.0Feb 2023View details →

Data for Whole-cell modeling of E. coli colonies enables quantification of single-cell heterogeneity in the antibiotic response

Data from simulations used to generate the figures in the paper Whole-cell modeling of E. coli colonies enables quantification of single-cell heterogeneity in the antibiotic response. To reproduce analyses, extract colony_data.zip in the data folder after cloning the vivarium-ecoli repository. The extracted folder contains the following items: <ul> <li>sim_dfs: a folder containing the CSV files that represent a subset of the raw simulation data used for downstream analyses.</li> <li>glc_10000_fluxome.csv: Each row represents a reaction in central carbon metabolism (in same order as listed in validation/ecoli/flat/toya_2010_central_carbon_fluxes.tsv). Each column represents a single time point for a single cell in a baseline glucose simulation (seed 10000). Each value is a flux (mmol/L/hr). Provided as input to ecoli/analysis/centralCarbonMetabolism.py script to reproduce fluxome validation plot.</li> <li>glc_10000_proteome_avgs.csv: Each row represents a protein monomer (in same order as sim_data.translation.monomer_data["id"] where sim_data is reconstruction/sim_data/kb/validationData.cPickle). Each column represents a cell in a baseline glucose simulation (seed 10000). Each row represents a protein monomer. Each value represents the average count of a given protein monomer for a given cell. Provided as input to ecoli/analysis/proteinCountsValidation.py script to reproduce proteome validation plot.</li> <li>glc_10000_expressome.csv: Each column represents a gene (with the exception of the final two metadata columns: "Time" and "Agent ID"). Each row represents a specific cell (agent) at a specific time in a baseline glucose simulation (seed 10000). Each value represents the number of new RNA transcripts for a given gene in a given cell at a given time. Provided as input to ecoli/analysis/antibiotics_colony/subgen_gene_plots/count_subgen.py script to calculate number of sub-generational genes among all genes and antibiotic response genes.</li> <li>glc_10000_total_mrna.json: Mapping of agent IDs for all cells in a baseline glucose simulation (seed 10000) to their average total mRNA count. Used by ecoli/analysis/antibiotics_colony/plot.py to generate Fig. 2C,D.</li> <li>jenner_2013.csv: Data extracted from Fig. 2C of <a href="https://doi.org/10.1073/pnas.1216691110">10.1073/pnas.1216691110</a>. Used by ecoli/analysis/antibiotics_colony/plot.py to generate Fig. S6A.</li> <li>olson_2006.csv: Data extracted from Fig. 2D of <a href="https://doi.org/10.1128%2FAAC.01499-05">10.1128/AAC.01499-05</a>. Used by ecoli/analysis/antibiotics_colony/plot.py to generate Fig. S6A.</li> <li>lysis_ratios.csv: Data extracted from Fig. 2 of <a href="https://doi.org/10.1099/00221287-31-3-339">10.1099/00221287-31-3-339</a>. Used by ecoli/analysis/antibiotics_colony/plot.py to generate Fig. 4N.</li> </ul>

IgLON5 immunoprecipitation files of binding partners in rat granullar cells of the cerebellum

Background: Anti-IgLON5 disease is a rare neurological disorder characterized by autoantibodies against IgLON5, and pathological evidence of neurodegeneration. IgLON5 is a cell adhesion molecule but its physiological function is unknown. Our aim was to investigate the IgLON5 interactome and to determine if IgLON5 antibodies (IgLON5-abs) affect these protein interactions. Methods: IgLON5 interactome was investigated by mass spectrometry sequencing of proteins immunoprecipitated by IgLON5 autoantibodies using cultures of rat cerebellar granular neurons (CGNs). Shedding of IgLON5 was explored using HEK cells transfected with human IgLON5 plasmid and in CGNs. Interactions of IgLON5 with identified binding partners and IgLON5-abs effects were confirmed by immunofluorescence in transfected HEK cells and rat hippocampal neurons. Results: Patients' IgLON5 antibodies co-precipitated all members of the IgLON family and three 3 additional surface proteins. IgLON5 predominantly establishes homomeric and heteromeric cis (within the cell) and trans (between cells)-interactions with other IgLON family members and undergoes spontaneous ectodomain shedding. Antibodies from patients with anti-IgLON5 disease prevent trans-interactions in hippocampal neurons independently of the IgLON5 IgG subclass distribution. Conclusions: We show a potentially novel pathogenic mechanism of IgLON5-abs that consists in blocking IgLON5 interactions with its binding partners. These findings extend our knowledge about the physiological role of IgLON5 and pave the way to future understanding of the pathological mechanisms of anti-IgLON5 disease.

Pretrained models and test examples of live cell anatomy segmentation

Pretrained models of live cell anatomy segmentation and test examples of the segmentation of 15 structures

opencc-by-4.0Jan 2022View details →

DeLTA 2.0: A deep learning pipeline for quantifying single-cell spatial and temporal dynamics

Datasets associated with https://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1009797

opencc-by-4.0Sep 2022View details →

Meta-analysis of (single-cell method) benchmarks reveals the need for extensibility and interoperability

Additional files for "Meta-analysis of (single-cell method) benchmarks reveals the need for extensibility and interoperability". Additional file 1 - list of benchmarks with consolidate survey answers (CSV) Additional file 2 - review form (CSV)  Additional file 3 - unedited (anonymized) survey responses (TXT)

The PLOD2/Succinate axis regulates the epithelial-mesenchymal plasticity and cancer cell stemness

Aberrant accumulation of succinate has been detected in many cancers. However, the cellular function and regulation of succinate in cancer progression is not completely understood. Using stable isotope-resolved metabolomics (SIRM) analysis, we showed that the epithelial mesenchymal transition (EMT) was associated with profound changes in metabolites, including the elevation of cytoplasmic succinate levels. Treatment with cell-permeable succinate induced mesenchymal phenotypes in mammary epithelial cells and enhanced cancer cell stemness. Chromatin immunoprecipitation (ChIP) and sequence analysis showed that elevated cytoplasmic succinate levels were sufficient to reduce global 5-hydroxymethylcytosinene (5hmC) accumulation and induce transcriptional repression of EMT-related genes. We showed that expression of procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 (PLOD2) was associated with an elevation of cytoplasmic succinate during the EMT process. Silence of PLOD2 expression in breast cancer cells reduced succinate levels and inhibited cancer cell mesenchymal phenotypes and stemness, which was accompanied by elevated 5hmC levels in chromatin. Importantly, exogenous succinate rescued cancer cell stemness and 5hmC levels in PLOD2-silenced cells, suggesting that PLOD2 promotes cancer progression at least partially through succinate. These results reveal the previously unidentified function of succinate in enhancing cancer cell plasticity and stemness.

Single-cell RNA-seq count data used in differential expression benchmark study

Count matrices and meta data tables from simulated and real world immune cell single-cell RNA-seq experiments. All files are in Rds format and can be read by R using "readRDS()".  <ul> <li>10k_*: These files contain a filtered version of the 10k Human PBMCs, 3' v3.1 data <a href="https://www.10xgenomics.com/resources/datasets/10k-human-pbmcs-3-v3-1-chromium-controller-3-1-high">published</a> by 10x Genomics</li> <li>blueprint_data.Rds: This file contains the bulk RNA-seq data downloaded from <a href="http://dcc.blueprint-epigenome.eu">BLUEPRINT</a></li> <li>blueprint_immune_comparisons.Rds: Results from running three bulk RNA-seq differential expression methods</li> <li>sim_data*: These files contain the count matrices and meta data tables for the simulated data. Every file contains a list of 13 replicates.</li> </ul>

Data from: Postnatal administration of S-adenosylmethionine restores developmental AHR activation-induced deficits in CD8+ T cell function during influenza A virus infection

Abstract Developmental exposures can influence life-long health; yet, counteracting negative consequences is challenging due to poor understanding of cellular mechanisms. The aryl hydrocarbon receptor (AHR) binds many small molecules, including numerous pollutants. Developmental exposure to the signature environmental AHR ligand 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) significantly dampens adaptive immune responses to influenza A virus (IAV) in adult offspring. CD8+ cytotoxic T lymphocytes (CTL) are crucial for successful infection resolution, which depends on the number generated and the complexity of their functionality. Prior studies showed developmental AHR activation significantly reduced the number of virus-specific CD8+ T cells, but impact on their functions is less clear. Other studies showed developmental exposure was associated with differences in DNA methylation in CD8+ T cells. Yet, empirical evidence that differences in DNA methylation are causally related to altered CD8+ T cell function is lacking. The two objectives were to ascertain whether developmental AHR activation affects CTL function, and whether differences in methylation contribute to reduced CD8+ T cell responses to infection. Developmental AHR triggering significantly reduced CTL polyfunctionality, and modified the transcriptional program of CD8+ T cells. S-adenosylmethionine (SAM), which increases DNA methylation, but not Zebularine, which diminishes DNA methylation, restored polyfunctionality and boosted the number of virus-specific CD8+ T cells. These findings suggest that diminished methylation, initiated by developmental exposure to an AHR-binding chemical, contributes to durable changes in antiviral CD8+ CTL functions later in life. Thus, deleterious consequence of development exposure to environmental chemicals are not permanently fixed, opening the door for interventional strategies to improve health.

Experimental data set for the article entitled "Mathematical Model of Steam Reforming in the Anode Channel of a Molten Carbonate Fuel Cell"

Experimental data for the article: Szablowski, L.; Dybinski, O.; Szczesniak, A.; Milewski, J. Mathematical Model of Steam Reforming in the Anode Channel of a Molten Carbonate Fuel Cell. Energies 2022, 15, 608. The experiments were performed by the first two authors. These data set refer to experiments carried out on a stand used to test high-temperature fuel cells. The subject of the study was a molten carbonate fuel cell fueled with a mixture of methane and steam with steam to carbon ratio of 2.0, 2.5, 3.0 and 3.5 and at the cell operating temperature of 550°C and 650°C. Additionally, in the anode channel of the cell, there was a catalyst in the amount of 2 g. The active area of the cell was 20.25 cm2. The article that uses these research results is published in an open access journal with a CC-BY license. This research was funded by the National Science Center, Poland (Grant number 2020/39/D/ST8/02021).

Extended Data for Single-cell Transcriptional Uncertainty Landscape of Cell Differentiation

The dataset includes supporting information for the study titled "Single-cell Transcriptional Uncertainty Landscape of Cell Differentiation".

Porcine cell-free system mass spectrometry compiled data sets

The degradation of sperm-borne mitochondria after fertilization is a conserved event. This process known as post-fertilization sperm mitophagy, ensures exclusively maternal inheritance of the mitochondria-harbored mitochondrial DNA genome. This mitochondrial degradation is in part carried out by the ubiquitin proteasome system. In mammals, ubiquitin-binding pro-autophagic receptors such as SQSTM1 and GABARAP have also been shown to contribute to sperm mitophagy. These systems work in concert to ensure the timely degradation of the sperm-borne mitochondria after fertilization. We hypothesize that other receptors, cofactors, and substrates are involved in post-fertilization mitophagy. Mass spectrometry was used in conjunction with a porcine cell-free system to identify other autophagic cofactors involved in post-fertilization sperm mitophagy. This porcine cell-free system is able to recapitulate early fertilization proteomic interactions. Altogether, 185 proteins were identified as statistically different between control and cell-free treated spermatozoa. Six of these proteins were further investigated, including MVP, PSMG2, PSMA3, FUNDC2, SAMM50, and BAG5. These proteins were phenotyped using porcine in vitro fertilization, cell imaging, proteomics, and the porcine cell-free system. The present data confirms the involvement of known mitophagy determinants in the regulation of mitochondrial inheritance and provides a master list of candidate mitophagy co-factors to validate in the future hypothesis-driven studies.

Dataset related to article: Cell-envelope growth of Gram-negative bacteria proceeds independently of cell-wall synthesis

Single-cell data for the article:  Enno R. Oldewurtel, Yuki Kitahara, Baptiste Cordier, Richard Wheeler, Gizem Özbaykal, Elisa Brambilla, Ivo Gomperts Boneca, Lars D. Renner, and Sven van Teeffelen Cell-envelope growth of Gram-negative bacteria proceeds independently of cell-wall synthesis. EMBO J (2023)

opencc-by-4.0May 2022View details →

Supporting data for "Morphological profiling by high-throughput single-cell biophysical fractometry"

No specific description. For more information, please contact: iriszqzh@connect.hku.hk.

opencc-by-4.0Oct 2022View details →

PARK15/FBXO7 is dispensable for PINK1/Parkin mitophagy in iNeurons and HeLa cell systems

The protein kinase PINK1 and ubiquitin ligase Parkin promote removal of damaged mitochondria via a feed-forward mechanism involving ubiquitin (Ub) phosphorylation, Parkin activation, and ubiquitylation of mitochondrial outer membrane proteins to support recruitment of mitophagy receptors. The ubiquitin ligase substrate receptor FBXO7/PARK15 is mutated in an early-onset parkinsonian-pyramidal syndrome. Previous studies have proposed a role for FBXO7 in promoting Parkin-dependent mitophagy. Here, we systematically examine the involvement of FBXO7 in depolarization-dependent mitophagy in the well-established HeLa and induced-neurons cell systems. We find that FBXO7-/- cells have no demonstrable defect in: 1) kinetics of pUb accumulation, 2) pUb puncta on mitochondria by super-resolution imaging, 3) recruitment of Parkin and autophagy machinery to damaged mitochondria, 4) mitophagic flux, and 5) mitochondrial clearance as quantified by global proteomics. Moreover, global proteomics of neurogenesis in the absence of FBXO7 reveals no obvious alterations in mitochondria or other organelles. These results argue against a general role for FBXO7 in Parkin-dependent mitophagy and point to the need for additional studies to define how FBXO7 mutations promote parkinsonian-pyramidal syndrome.

opencc-zeroApr 2023View details →

Data from: Pooled genome-wide CRISPR activation screening for rapamycin resistance genes in Drosophila cells

Loss-of-function and gain-of-function genetic perturbations provide valuable insights into gene function. In Drosophila cells, while genome-wide loss-of-function screens have been extensively used to reveal mechanisms of a variety of biological processes, approaches for performing genome-wide gain-of-function screens are still lacking. Here, we describe a pooled CRISPR activation (CRISPRa) screening platform in Drosophila cells and apply this method to both focused and genome-wide screens to identify rapamycin resistance genes. The screens identified three genes as novel rapamycin resistance genes: a member of SLC16 family of monocarboxylate transporters (CG8468), a member of the lipocalin protein family (CG5399), and a zinc finger C2H2 transcription factor (CG9932). Mechanistically, we demonstrate that CG5399 overexpression activates the RTK-Akt-mTOR signaling pathway and that activation of insulin receptor (InR) by CG5399 requires cholesterol and clathrin-coated pits at the cell membrane. This study establishes a novel platform for functional genetic studies in Drosophila cells.

opencc-by-4.0Apr 2023View details →

Engineering cellular communication between light-activated synthetic cells and bacteria (Source data)

Source data files for supplementary figures for published version of "Engineering cellular communication between light-activated synthetic cells and bacteria" https://www.biorxiv.org/content/10.1101/2022.07.22.500923v1

opencc-by-4.0Apr 2023View details →

Design of a triaxial compression cell for x-ray tomography

This repository contains the technical drawings of the triaxial compression setup developed and used in the PhD thesis "E<a href="https://hal.science/tel-04202827v1">xperimental investigation of the effects of particle shape and friction on the mechanics of granular media</a>" by Gustavo Pinzón (Université Grenoble Alpes). The setup is a modification of the traditional setup used in geotechnical testing, presenting a sliding base on one of the ends, which allows the creation of a singular strain localisation region. The experimental setup is designed for in-situ testing inside the x-ray tomography cabin of Laboratoire 3SR, Grenoble, France.  The Readme.md file contains further details on the structure of the repository and the materials of each component. Please refer to the PhD thesis <a href="https://hal.science/tel-04202827v1">here</a> for further details not found in the Readme.md file. Additional information/data not included in this repository is available upon request.