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119 results for “Colletotrichum”
FIGURE 1 in Two new endophytic fungi of Colletotrichum and Diaporthe isolated from Berchemia polyphylla var. leioclada in Guizhou Province, China
FIGURE 1. Colletotrichum berchemiae (ex-type culture, GMBCC 20001). a, b Front and reverse colony on PDA. c–e Conidiomata on PDA. h–j Conidiophores. k–m Conidiogenous cells and conidia. n–p Conidia. Scale bars: f = 100 μm; g–i, n = 20 μm, j, k = 30um, l, m, o, p = 10 um.
FIGURE 3 in Two new endophytic fungi of Colletotrichum and Diaporthe isolated from Berchemia polyphylla var. leioclada in Guizhou Province, China
FIGURE 3. Results of the PHI test of Colletotrichum berchemiae and closely related species using both LogDet transformation and splits decomposition. The PHI test results (Φw) <0.05 indicate significant recombination within the dataset. The new taxa are in red.
FIGURE2. Colletotrichum serranegrense COAD 2100. A in Colletorichum serranegrense sp. nov., a new endophytic species from the roots of the endangered Brazilian epiphytic orchid Cattleya jongheana
FIGURE2. Colletotrichum serranegrense COAD 2100. A, Setae; B, Appressoria; C, Conidiophores; D, Conidia. Scale bars =10 μm.
FIGURE 3 in A new species of Colletotrichum from Sonchus sp. in Italy
FIGURE 3. Results of the pairwise homoplasy index (PHI) test of closely related species using both LogDet transformation and splits decomposition. PHI test results (Φw) <0.05 indicate significant recombination within the dataset.
FIGURE 2 in A new species of Colletotrichum from Sonchus sp. in Italy
FIGURE 2. Colletotrichum sonchicola (holotype). A–B. Appearance of conidiomata on host. C. Setae. D–F. Apex of setae. G. Conidiophores. H. Conidiogenous cells. I–K. Conidia. L–M. Appressoria. N. Upper view of 7 d old culture. O. Reverse view of 7 d old culture. Scale bars: A–B = 0.2 mm, C, G = 20 μm, D–F, H–M = 10 μm.
FIGURE 1 in A new species of Colletotrichum from Sonchus sp. in Italy
FIGURE 1. One of the 100 most parsimonious trees obtained from a heuristic search of combined ITS, GAPDH, CHS, ACT and β-tubulin sequenced data of dematium species complex. Parsimony bootstrap support values greater than or equal to 75% and Bayesian posterior probabilities above 0.80 are indicated above the nodes. The ex-type strains are in bold, isolates of this study are in green. The scale bar indicates forty changes. The tree is rooted with C. nigrum CBS 169.49.
FIGURE 3 in Colletotrichum yulongense sp. nov. and C. rhombiforme isolated as endophytes from Vaccinium dunalianum var. urophyllum in China
FIGURE 3. Result of the pairwise homoplasy index (PHI) test of closely related species using both LogDet transformation and splits decomposition. PHI test results (Φ w) <0.05 indicate significant recombination within the dataset.
FIGURE 5 in Colletotrichum yulongense sp. nov. and C. rhombiforme isolated as endophytes from Vaccinium dunalianum var. urophyllum in China
FIGURE 5. Colletotrichum rhombiforme (CFCC 50823) A, B. Upper and reverse sides of cultures on PDA 7 days after inoculation. C. Conidiogenous cells on SNA. D. Seta on PDA. E. Appressorium in slide culture. F. Conidia on SNA. Bars: C–F = 10 μm.
FIGURE 2 in Colletotrichum yulongense sp. nov. and C. rhombiforme isolated as endophytes from Vaccinium dunalianum var. urophyllum in China
FIGURE 2. Phylogenetic tree derived from maximum parsimony analysis of the combined ACT, CAL, CHS-1, GAPDH, ITS, TUB2 and GS genes of C. gloeosporioides species complex, using C. boninense MAFF 305972* and C. hippeastri CBS 241.78* as outgroups. Bootstrap values of more than 70 from 1000 replications are shown above the respective branches. Bayesian posterior probabilities (PP) were estimated and clades with PP> 0.95 are marked under the branches. The new species, Colletotrichum yulongense, is in bold.
FIGURE 1 in Colletotrichum yulongense sp. nov. and C. rhombiforme isolated as endophytes from Vaccinium dunalianum var. urophyllum in China
FIGURE 1. Phylogenetic tree derived from maximum parsimony analysis of the combined ITS, GAPDH, CHS-1, HIS3 ACT and TUB2 sequences of C. acutatum species complex, using C. orchidophilum CBS 632.80* as outgroup. Bootstrap values of more than 70 from 1000 replications are shown above the respective branches. Bayesian posterior probabilities (PP) were estimated and clades with PP> 0.95 are marked under the branches. The newly sequenced C. rhombiforme is in bold.
FIGURE 4 in Colletotrichum yulongense sp. nov. and C. rhombiforme isolated as endophytes from Vaccinium dunalianum var. urophyllum in China
FIGURE 4. Colletotrichum yulongense (from ex-holotype) A, B. Upper and reverse sides of cultures on PDA 7 days after inoculation. C. Conidiogenous cells on SNA. D. Seta on PDA. E. Conidia on SNA. F. Appressoria in slide culture. Bars: C–F = 10 μm.
Genome assembly and annotation files of Colletotrichum graminicola strain TZ-3
<p>The maize anthracnose stalk rot and leaf blight diseases caused by the fungal pathogen Colletotrichum graminicola is emerging as an important threat to corn production worldwide. In this work, we provide an improved genome assembly of C. graminicola strain TZ-3 by using the PacBio Sequel II and Illumina high-throughput sequencing technologies. We hope that this genome information will facilitate the pathogenesis research of this plant pathogen.</p>
FIGURE 2. Colletotrichum menezesiae COAD3523 in Colletotrichum menezesiae sp. nov.: a novel species causing anthracnose on chayote in Brazil
FIGURE 2. Colletotrichum menezesiae COAD3523, ex-holotype culture. A–B. Cultures on PDA, 7 d growth, from above (A) and below (B). C, E. Conidiophores and conidiogenous cells from PDA (C) and Sicyos edulis (E). F–G. Appressoria. H–I. Setae on S. edulis. D, J. Conida from PDA (D) and S. edulis (F). Scale bars: F–G, 5 µm; 10 µm for the others.
FIGURE 1 in Colletotrichum menezesiae sp. nov.: a novel species causing anthracnose on chayote in Brazil
FIGURE 1. Maximum likelihood tree of the Colletotrichum orbiculare species complex inferred from a concatenated alignment of ACT, GAPDH, nrITS and TUB2. Isolates from the present study are highlighted in bold. Ex-types are indicated with a "T" after the taxon label. Significant bootstrap (≥70%) and posterior probabilities (≥0.95) values are shown above the branches. The scale bar indicates the estimated number of substitutions per site. The tree is rooted with C. magnum.
Fig. 1 in Brevianthrones, bianthrones from a Chinese isolate of the endophytic fungus Colletotrichum brevisporum
Fig. 1. Structures of brevianthrones and anthraquinones isolated from cultures of Colletotrichum brevisporum.
Fig. 7 in Early transcriptional response of terpenoid metabolism to Colletotrichum gloeosporioides in a resistant wild strawberry Fragaria nilgerrensis
Fig. 7. Phylogenetic relationship of the five FnTPS candidates with other known terpene synthases and a sequence alignment showing the conserved protein motifs. (A) A maximum-likelihood tree of the TPS proteins depicting the TPS-a, TPS-b, TPS-d, TPS-e/f, and TPS-g clades, with bootstrap values greater than 50% shown for the branching. The scale bar corresponds to 6% amino acid substitution. The five F. nilgerrensis proteins are in red. Selected proteins with available three-dimensional structural data are shown in bold using their PDB code followed by the abbreviated species name. The known enzymatic products are in light blue followed with the UniProt Accession numbers of the proteins. (B) Alignment of the five candidates FnTPSs with α-farnesene synthase from apple (Malus domestica) and α-bergamotene synthase from Lavender (Lavandula Angustifolia). The DxDD motif of typical class II terpene synthases in FnTPS6 is boxed and the highly conserved class I DDxxD as well as the lesser conserved RRx8W and NSE/DTE motifs are indicated. The color regime of amino acids is set in Bioedit version 7.2.6. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Fig. 3 in Early transcriptional response of terpenoid metabolism to Colletotrichum gloeosporioides in a resistant wild strawberry Fragaria nilgerrensis
Fig. 3. Most enriched metabolic pathways of the DEGs during the first 18 hpi with C. gloeosporioides. Only significant pathways with a Q-value below 1 are shown. Terpene metabolism clusters are highlighted.
Fig. 1 in Early transcriptional response of terpenoid metabolism to Colletotrichum gloeosporioides in a resistant wild strawberry Fragaria nilgerrensis
Fig. 1. Determination of terpenoids in leaves challenged with C. gloeosporioides. (A) Total contents of monoterpenes and sesquiterpenes, (B–C) The contents of individual monoterpene and sesquiterpene identified. Data are average of two independent experiments.
Fig. 4 in Early transcriptional response of terpenoid metabolism to Colletotrichum gloeosporioides in a resistant wild strawberry Fragaria nilgerrensis
Fig. 4. Schematic illustration of general terpenoid biosynthesis pathways showing identified unigenes from F. nilgerrensis leaf transcriptome data. The interconvertible precursors IPP and DMAPP, two phosphorylated C5 unites, are produced by the MVA and MEP pathways which are exchangeable from both compartmentations. The chloroplast is a major site for synthesis of hemiterpene (C5), monoterpenoids (C10), diterpenoids (C20) carotenoids (C40) and chlorophyll, while the cytosol and other organelle are responsible for synthesis of monoterpenoids (C10), sesquiterpene (C15) and triterpene (C30). But that is not strictly conclusive. Arrow with lines indicate reactions catalyzed by enzymes and the encoding genes, with unigenes identified in this experiment boxed. The color highlights are for better visualization. Abbreviations: AACT, acetoacetyl-CoA thiolase; CMK, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase; DXR, 1-deoxy-D-xylulose 5-phosphate reductase; DXS, 1-deoxy-D-xylulose 5-phosphate synthase; HDR, (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate reductase; HDS, (E)-4-hydroxy-3- methyl-but-2-enyl diphosphate synthase; HMGR, 3-hydroxy-3-methylglutaryl-CoA reductase; HMGS, 3-hydroxy-3-methylglutaryl-CoA synthase; IDI, isopentenyl diphosphate isomerase; MCT, MEP cytidyltransferase; MDC, mevalonate-5-diphosphate decarboxylase; MDS, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MVK, mevalonate kinase. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Fig. 2 in Early transcriptional response of terpenoid metabolism to Colletotrichum gloeosporioides in a resistant wild strawberry Fragaria nilgerrensis
Fig. 2. Global differentially-expressed-genes (DEG) in leaves of F. nilgerrensis after challenged by C. gloeosporioides. (A) DEG heatmap depicts clustering of the early upregulated genes (up to 18-h post challenge) and the late respondents (>24 h) in the upper and lower parts, respectively, with top GO terms indicated. (B) Major GO term enrichment in an early responsive gene cluster 7 showing that terpenoid biosynthesis genes are co-expressed with antipathogen immune-responsive pathway genes. Each time-point contains 2 biological replications and the expression data was zero-normalized before hierarchical clustering is performed (Pearson uncentered metric, average linkage ordering) using Cluster 3.0 version 1.58. Heatmap was examined and generated using Java Tree- View version 1.1.6r4. Gene Ontology (GO) enrichment analysis of the DEGs is implemented by the topGO R package-based Kolmogorov–Smirnov test.
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