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58 results for “Crossing experiments”
FIGURE 5 in Chlamydomonas schloesseri sp. nov. (Chlamydophyceae, Chlorophyta) revealed by morphology, autolysin cross experiments, and multiple gene analyses
FIGURE 5. Secondary structures of ITS-1 rDNA sequences among the species of Chlamydomonas, A. C. schloesseri, B. C. incerta, and C. C. reinhardtii. CBCs and HCBCs are marked in black and grey, respectively.
FIGURE 1 in Chlamydomonas schloesseri sp. nov. (Chlamydophyceae, Chlorophyta) revealed by morphology, autolysin cross experiments, and multiple gene analyses
FIGURE 1. Morphology and phenotypic plasticity of Chlamydomonas schloesseri sp. nov. A–J. vegetative cells, arrow in F. marked the eyespot; K–O. different sporangia; scale bar = 10 μm.
FIGURE 8 in Chlamydomonas schloesseri sp. nov. (Chlamydophyceae, Chlorophyta) revealed by morphology, autolysin cross experiments, and multiple gene analyses
FIGURE 8. Comparison of the amino acid composition of the rbcL gene among the species of Chlamydomonas. The IUPAC symbols were used for the amino acids.
FIGURE 4 in Chlamydomonas schloesseri sp. nov. (Chlamydophyceae, Chlorophyta) revealed by morphology, autolysin cross experiments, and multiple gene analyses
FIGURE 4. Molecular phylogeny of Chlamydomonas and representatives belonging to the Tetrabaenaceae and Goniaceae based on rbcL and ITS rDNA sequence comparisons. The phylogenetic trees shown were inferred using the maximum likelihood method based on the data sets (29 taxa: 915 aligned positions for ITS, 1128 for rbcL) using PAUP 4.0b10. For the analyses the best model was calculated by Modeltest 3.7. The setting of the best model was given as follows: (ITS) GTR+I+G (base frequencies: A 0.2634, C 0.2225, G 0.2192, T 0.2949; rate matrix A-C 1.2503, A-G 2.1014, A-U 2.0643, C-G 0.3957, C-U 3.3009, G-U 1.0000) with the proportion of invariable sites (I = 0.2682) and gamma shape parameter (G = 0.6858); (rbcL) GTR+I+G (base frequencies: A 0.2777, C 0.1688, G 0.2048, T 0.3487; rate matrix A-C 0.2771, A-G 3.5238, A-U 4.0693, C-G 0.6646, C-U 7.4921, G-U 1.0000) with the proportion of invariable sites (I = 0.5523) and gamma shape parameter (G = 0.6480). The branches in bold are highly supported in all analyses (Bayesian values> 0.95 calculated with PHASE and MrBayes; bootstrap values> 70% calculated with PAUP using maximum likelihood, neighbor-joining, maximum parsimony and RAxML using maximum likelihood). The Goniaceae are only weakly supported in bootstrap and Bayesian analyses, which is indicated by an asterisk.
FIGURE 7 in Chlamydomonas schloesseri sp. nov. (Chlamydophyceae, Chlorophyta) revealed by morphology, autolysin cross experiments, and multiple gene analyses
FIGURE 7. Comparison of the conserved region of ITS-2 among the species of Chlamydomonas. Extraction of this region and translation into a number code for its usage as barcode (extracted bases highlighted with an asterisk). Number code for each base pair: 1 = A-U; 2 = U-A; 3 = G-C; 4 = C-G; 5 = G•U; 6 = U•G; 7 = mismatch.
FIGURE 6 in Chlamydomonas schloesseri sp. nov. (Chlamydophyceae, Chlorophyta) revealed by morphology, autolysin cross experiments, and multiple gene analyses
FIGURE 6. Secondary structures of ITS-2 rDNA sequences among the species of Chlamydomonas, A. C. schloesseri, B. C. incerta, and C. C. reinhardtii. CBCs and HCBCs are marked in black and grey, respectively.
FIGURE 3 in Chlamydomonas schloesseri sp. nov. (Chlamydophyceae, Chlorophyta) revealed by morphology, autolysin cross experiments, and multiple gene analyses
FIGURE 3. Molecular phylogeny of Chlamydomonas and representatives belonging to the Tetrabaenaceae, Goniaceae and Volvocaceae based on SSU and ITS rDNA sequence comparisons. The phylogenetic trees shown were inferred using the maximum likelihood method based on the data sets (2688 aligned positions of 75 taxa) using PAUP 4.0b10. For the analyses the best model was calculated by Modeltest 3.7. The setting of the best model was given as follows: GTR+I+G (base frequencies: A 0.2516, C 0.2232, G 0.2514, T 0.2738; rate matrix A-C 1.3107, A-G 2.3565, A-U 2.4706, C-G 0.4123, C-U 4.2284, G-U 1.0000) with the proportion of invariable sites (I = 0.6048) and gamma shape parameter (G = 0.3904). The branches in bold are highly supported in all analyses (Bayesian values> 0.95 calculated with PHASE and MrBayes; bootstrap values> 70% calculated with PAUP using maximum likelihood, neighbor-joining, maximum parsimony and RAxML using maximum likelihood). The Goniaceae are only moderated supported in bootstrap and Bayesian analyses, which is indicated by an asterisk.
FIGURE 2 in Chlamydomonas schloesseri sp. nov. (Chlamydophyceae, Chlorophyta) revealed by morphology, autolysin cross experiments, and multiple gene analyses
FIGURE 2. Lysis of sporangium cell wall under influence of the VLE autolysin in Chlamydomonas schloesseri (SAG 2485); scale bar = 10 μm.
Data from: Performance of primary care in different health care facilities: a cross sectional study of patients' experiences in Southern Malawi
Objective: In most African countries, primary care is delivered through a district health system. Many factors, including staffing levels, staff experience, availability of equipment and facility management, affect the quality of primary care between and within countries. The purpose of this study was to assess the quality of primary care in different types of public health facilities in Southern Malawi. Study design: This was a cross sectional quantitative study. Setting: The study was conducted in 12 public primary care facilities in Neno, Blantyre and Thyolo districts in July 2018. Participants: Patients aged 18 years and above, excluding the severely ill, were selected to participate in the study. Primary outcomes: We used the Malawian primary care assessment tool to conduct face-to-face interviews. ANOVA at 0.05 significance level was performed to compare primary care dimension means and total primary care scores. Linear regression models at 95% CI were used to assess associations between primary care dimension scores, patients' characteristics and healthcare setting. Results: The final number of respondents was 962 representing 96.1% response rate. Patients in Neno hospitals scored 3.77 points higher than those in Thyolo health centers, and 2.87 higher than those in Blantyre health centers in total primary care performance. Primary care performance in health centers and in hospital clinics was similar in Neno (20.9 vs 19.0, p= 0.608) while in Thyolo, it was higher at the hospital than at the health centers (19.9 vs 15.2, p<0.001). Urban and rural facilities showed a similar pattern of performance. Conclusion: These results showed considerable variation in experiences among primary care users in the public health facilities in Malawi. Factors such as funding, policy and clinic level interventions influence patients' reports of primary care performance. These factors should be further examined in longitudinal and experimental settings.
Dataset for state-specific reaction of OCS+Ne* from "A novel crossed-molecular-beam experiment for investigating reactions of state- and conformationally selected strong-field-seeking molecules"
<p><strong>Data and analysis for all figures containing experimental data of the paper:</strong></p> <p>•Title: A novel crossed-molecular-beam experiment for investigating reactions of state- and conformationally selected strong-field-seeking molecules</p> <p>•Authors: L. Ploene, P. Straňák, H. Gao, J. Küpper and S.Willitsch</p> <p>•Journal: Molecular Physics</p> <p>DOI: https://doi.org/10.1080/00268976.2021.1965234</p> <p> </p> <p><strong>The PDF contains three flow-chart diagrams, describing how the data has been analysed:</strong></p> <p>•Diagram 1: Deflection profiles of OCS by femtosecond-ionisation compared to MC trajectory simulations for Paper-Figure 3</p> <p>•Diagram 2: Typical TOF-MS trace of Ne* + OCS for Paper-Figure 4 and Experimental deflection profile of OCS+ and S+ from chemi-ionisation reaction Ne* + OCS for Paper-Figure 5</p> <p>•Diagram 3: Least-square fitting of experimental deflection profiles of OCS+and S+ with state-specific simulated deflection profiles for Paper-Figure 6a and 6b</p>
Supplemental Table S3. Least square means of the digestible (d) AA (Leu, Ile, Arg, Phe, Thr and Val) supply relative to the cow's requirements (%) for the CTRL, MetLys and MetLysHis groups in the depletion, RP-AA and cross-back period of the trial. Calculations based on the actual DMI, MY and BW of the cows during the experiment.
<p><strong>Supplemental Table S3.</strong> Least square means of the digestible (d) AA (Leu, Ile, Arg, Phe, Thr and Val) supply relative to the cow’s requirements (%) for the CTRL, MetLys and MetLysHis groups in the depletion, RP-AA and cross-back period of the trial. Calculations based on the actual DMI, MY and BW of the cows during the experiment.</p>
The Experiences of Family Caregivers of Stroke Patients: A Cross-sectional Study of a French Cohort
ClinicalTrials.gov study NCT04741451. IPD Sharing: Not stated. Countries: 1. Publications: 2.
Data from: Are blue eggs a sexually selected signal of female collared flycatchers? A cross-fostering experiment
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Data from: Disparity in preemptive end-of-life conversation experience caused by subjective economic status among general Japanese elderly people: a cross-sectional study with stratified random sampling
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Data from: Performance of primary care in different health care facilities: a cross sectional study of patients’ experiences in Southern Malawi
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Data from: Out-of-hospital endotracheal intubation experience, confidence, and confidence-associated factors among Northern Japanese emergency life-saving technicians: a population-based cross-sectional study
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Data from: The influence of social structure on brood survival and development in a socially polymorphic ant: insights from a cross-fostering experiment
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Infection data and spore counts for cross inoculation experiments
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Data from: Induced parental care in a poison frog: a tadpole cross-fostering experiment
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Spatial Plus Cross-Validation experiments datasets and codes (new)
<p>This rar/zip file includes all materials of Spatial Plus Cross-Validation experiments. </p> <p>They are ordered by the first number of folder's name.</p> <p>In each folder, the order of running code scripts are labeled by the first number of code's name.</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.