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Fig. 6 in Histopathological characterisation of retinal lesions associated to Diplostomum species (Platyhelminthes: Trematoda) infection in polymorphic Arctic charr Salvelinus alpinus

Fig. 6. Edge of one of the vesicles produced by the accumulation of parasites. Retinal pigment layer and rods and cones layer display a progressive alteration in their structure and finally both layers become detached. Notice the reduction of the thickness of the RPE (arrow) in the vesicle. Scale bar = 300 μm.

Fig. 12 in Histopathological characterisation of retinal lesions associated to Diplostomum species (Platyhelminthes: Trematoda) infection in polymorphic Arctic charr Salvelinus alpinus

Fig. 12. PP-morphs. Diffuse changes in the posterior retina affecting mainly the RPE layer suggesting potential healing. Scale bar = 200 μm.

Fig. 4 in Histopathological characterisation of retinal lesions associated to Diplostomum species (Platyhelminthes: Trematoda) infection in polymorphic Arctic charr Salvelinus alpinus

Fig. 4. Diplostomum sp. metacercaria within the retinal structures. This specimen is clearly placed between the retinal pigmented epithelium (RP) and rod and cones layer (RC) creating a small space between them and the parasite. Damaged retinal pigment epithelium is clearly observed and also rod and cone layer display morphological alterations. Scale bar = 200 μm.

Fig. 11. A in Histopathological characterisation of retinal lesions associated to Diplostomum species (Platyhelminthes: Trematoda) infection in polymorphic Arctic charr Salvelinus alpinus

Fig. 11. A single Diplostomum sp. metacercaria within the posterior retina with scarce development of surrounding vesicle and mechanical compression against the RPE and the cones and rods layers. Scale bar = 200 μm.

Fig. 7 in Histopathological characterisation of retinal lesions associated to Diplostomum species (Platyhelminthes: Trematoda) infection in polymorphic Arctic charr Salvelinus alpinus

Fig. 7. Early lesions in RPE and RC in the retina closer to the edge of the vesicles. Cones and rods display a disorganized pattern between the pigmented processes of the RPE. Scale bar = 100 μm.

Fig. 2 in Histopathological characterisation of retinal lesions associated to Diplostomum species (Platyhelminthes: Trematoda) infection in polymorphic Arctic charr Salvelinus alpinus

Fig. 2. Vesicle with several Diplostomum specimens in a histological section. Vesicles are typically located near the ciliary body/retina contact area. C: cornea. I: iris. H/E. Scale bar = 1 mm.

Fig. 5 in Histopathological characterisation of retinal lesions associated to Diplostomum species (Platyhelminthes: Trematoda) infection in polymorphic Arctic charr Salvelinus alpinus

Fig. 5. Large vesicle with sections of many Diplostomum specimens. The vesicle clearly creates a large space between RP and RC. Scale bar = 400 μm.

Fig. 4 in Resolution of the identity of three species of Diplostomum (Digenea: Diplostomidae) parasitising freshwater fishes in South Africa, combining molecular and morphological evidence

Fig. 4. Metacercariae of Diplostomum spp. from eye lenses of different fish hosts; (a) Diplostomum sp. from Tilipia sparrmanii, live, ventral view (b) Diplostomum sp. from Tilipia sparrmanii, fixed, ventral view (c) Diplostomum sp. from Tilipia sparrmanii, live, sunken pseudosuckers (arrowhead) (hologenophore, GenBank MN813526, MN813534 and MN808616) (d) Diplostomum sp. 14 sensu Locke et al. (2015) from Synodontis zambezensis, live, ventral view (hologenophore, GenBank MN813541) (e) Diplostomum sp. 14 sensu Locke et al. (2015) from Oreochromis mossambicus, fixed, ventral view, small excretory granules (arrowhead) (hologenophore, GenBank MN813531, MN813539 and MN808621) (f) Diplostomum sp. 14 sensu Locke et al. (2015) from Synodontis zambezensis, fixed, ventral view, large excretory granules (arrowhead) (hologenophore, GenBank MN813541) (g) Diplostomum sp. 16 sensu Locke et al. (2015) from Pseudocrenilabrus philander, fixed, ventral view, everted pseudosuckers (arrowhead) (hologenophore, GenBank MN813532, MN813547 and MN808627) (h) Diplostomum sp. 16 sensu Locke et al. (2015) from Pseudocrenilabrus philander, fixed, ventral view, inverted pseudosuckers (arrowhead) (hologenophore, GenBank MN813533, MN813548 and MN808628) (i) Diplostomum sp. 16 sensu Locke et al. (2015) from Pseudocrenilabrus philander, live metacercariae inside of fish lens. Scale bars: a–h = 100 μm; i = 700 μm.

Fig. 3 in Resolution of the identity of three species of Diplostomum (Digenea: Diplostomidae) parasitising freshwater fishes in South Africa, combining molecular and morphological evidence

Fig. 3. Bayesian inference (BI) and maximum likelihood (ML) phylogram reconstructed using cox1 sequences for species of Diplostomum. Nodal support from BI and ML analyses indicated as BI/ML; only values> 0.90 (BI) and> 70 (ML) are displayed. Scale-bar indicates the expected number of substitution per site. Sequences generated in this study are in bold and indicated by blue rectangles. Codes with isolate information for newly generated sequences are provided in Table 3. Sequences derived from Africa are highlighted in blue, from Asia in purple, from Europe in orange, from North America in green (according to the map) and sequences reported from more than one continent are highlighted in black. Black arrows on the map demonstrate distribution of Diplostomum spathaceum and 'D. mergi Lineage 2' in both, Asia and Europe, and Diplostomum sp. 14 and Diplostomum sp. 16 in both, Africa and Asia. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 2 in Resolution of the identity of three species of Diplostomum (Digenea: Diplostomidae) parasitising freshwater fishes in South Africa, combining molecular and morphological evidence

Fig. 2. Bayesian inference (BI) and maximum likelihood (ML) phylograms reconstructed using (a) partial 28S rDNA sequences (b) ITS1-5.8S-ITS2 sequences for species of Diplostomum. Nodal support from BI and ML analyses indicated as BI/ML; only values> 0.90 (BI) and> 70 (ML) are displayed. Scale-bar indicates the expected number of substitution per site. Sequences generated in this study are in bold and indicated by blue rectangles. Codes with isolate information for newly generated sequences are provided in Table 3. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 1 in Resolution of the identity of three species of Diplostomum (Digenea: Diplostomidae) parasitising freshwater fishes in South Africa, combining molecular and morphological evidence

Fig. 1. Map illustrating the sampling localities on (a) River Riet in Mokala National Park (b) River Phongolo (Site 1, Site 2 and Nyamithi Lake) and the River Usuthu (Shokwe Pan) in Ndumo Game Reserve and (c) River Mooi (Boskop Dam) in Boskop Dam Nature Reserve, South Africa. The illustration was compiled in ArcGIS 10.6 (Available from https://support.esri.com/en/downloads).

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Fig. 13 in Histopathological characterisation of retinal lesions associated to Diplostomum species (Platyhelminthes: Trematoda) infection in polymorphic Arctic charr Salvelinus alpinus

Fig. 13. Diplostomum sp. metacercaria in a choroidal vessel. Scale bar = 200 μm.