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40 results for “Freeze-thaw”
A Randomized Controlled Study of Prolonging the Time of Progesterone Supplementation to Improve the Pregnancy Outcome of Single Day 6 Blastocyst Transfer of Freeze-thaw Cycle
ClinicalTrials.gov study NCT04938011. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Metabolic cost of freeze-thaw and source of CO2 production in the freeze-tolerant cricket Gryllus veletis
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Thermal adaptations to extreme freeze-thaw cycles in the high tropical Andes
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Data from: Autumn freeze-thaw events carry over to depress late-winter reproductive performance in Canada Jays
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Probabilistic Freeze-Thaw Record for the Northern Hemisphere, 2016-2020
This dataset provides a probabilistic freeze/thaw (FT) data record from 2016 to 2020 for the Northern Hemisphere derived using a deep learning model (U-Net). The model was informed by satellite multi-frequency microwave brightness temperature retrievals from the NASA SMAP (Soil Moisture Active Passive) and JAXA AMSR2 (Advanced Microwave Scanning Radiometer 2) radiometers, and trained using daily soil temperature observations from Northern Hemisphere weather stations and global reanalysis data (ERA-5). Unlike other available FT data records that provide only a binary classification of frozen or non-frozen conditions, this product includes both binary FT and continuous variable estimates of the probability of thawed conditions. This product is designed to complement other established binary FT data records, including the NASA FT Earth System Data Record and SMAP Level 3 FT operational products, by providing a probabilistic FT variable with enhanced accuracy and sensitivity to near-surface (<=5 cm depth) soil FT condition. The data are provided in cloud optimized GeoTIFF (COG) format.
scFTD-seq: freeze-thaw lysis based, portable approach toward highly distributed single-cell 3’ mRNA profiling
GEO Series GSE109535. Homo sapiens; Mus musculus. 9 samples. Type: Expression profiling by high throughput sequencing.
Effects of Freeze-Thawing and Intravenous Infusion on Mesenchymal Stromal Cell Gene Expression
GEO Series GSE76081. Homo sapiens; Mus musculus. 10 samples. Type: Expression profiling by high throughput sequencing.
The effect of freeze-thaw cycles on gene expression levels in lymphoblastoid cell lines
GEO Series GSE58942. Homo sapiens. 187 samples. Type: Expression profiling by array.
Data from: Indices of immune function used by ecologists are mostly unaffected by repeated freeze-thaw cycles and methodological deviations
Background: Over the past couple of decades, measuring immunological parameters has become widespread in studies of ecology and evolution. A combination of different immunological indices is useful for quantifying different parts of the immune system and comprehensively assessing immune function. Running multiple immune assays usually requires samples to be repeatedly thawed and re-frozen. There is some evidence that repeated freezing and thawing can affect assay results, but this has never been comprehensively studied in some common ecological immunology assays. We tested the effect of multiple (1, 2, 3, 4, 5, 10) freeze-thaw cycles on the results of four commonly used immunological assays: haemolysis-haemagglutination titres, haptoglobin concentration, bacterial killing capacity and total immunoglobulins (IgY). We tested five different bird species from four different bird orders (Passeriformes, Columbiformes, Charadriiformes and Galliformes), and we included both captive and free-living individuals. In addition, we tested for haptoglobin concentrations and the haemolysis-haemagglutination assay if re-analysing samples 1 year apart led to different results. For the haemolysis-haemagglutination assay we also tested two different sources of rabbit blood, and we compared untreated microtitre plates with plates that were "blocked" to prevent nonspecific interactions between the plate and assay reagents. Results: Repeated freezing and thawing of plasma had no effect on lysis titres, haptoglobin concentrations, bacterial killing capacity, or total immunoglobulin levels. Agglutination titres were unaffected by up to five cycles but were lower after ten freeze-thaw cycles. For the haemolysis-haemagglutination assay and haptoglobin concentrations, re-analysing samples 1 year apart yielded highly correlated data. For the haemolysis haemagglutination assay,thesourceofrabbit blood did not influence the results, and the untreated vs. blocked plates differed slightly overall, but at the individual level assay results were highly correlated. Using different rabbit blood sources or different types of microtitre plates yielded highly correlated data.Conclusions: Our data suggest that repeated freeze-thaw cycles do not impair assay results to the point of influencing ecological or evolutionary conclusions. Plasma samples can be safely stored in one tube and thawed repeatedly for different assays. Nevertheless, we recommend consistent treatment of samples in terms of freeze-thaw cycles or other laboratory treatments to minimize the potential for introducing a systematic bias.
Effect of Low Molecular Weight Heparin in Freeze-thaw Embryo Transfer Cycles in Women With Recurrent Implantation Failure
ClinicalTrials.gov study NCT06991530. IPD Sharing: NO. Countries: 1. Publications: 0.
Data from: Indices of immune function used by ecologists are mostly unaffected by repeated freeze-thaw cycles and methodological deviations
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A systematic evaluation of isolation techniques and freeze-thaw effects on plasma extracellular vesicle heterogeneity and subpopulation profiling
GEO Series GSE283848. Homo sapiens. 72 samples. Type: Other.
Tumor analysis: Freeze-thawing cycle of triple negative breast cancer cells changes tumor CD24/CD44 profile and percentage of tumor-infiltrating immune cells
GEO Series GSE114359. Homo sapiens. 6 samples. Type: Expression profiling by array.
Insufficiency of Copper Ion Homeostasis Causes Freeze-Thaw Injury of Yeast Cells
GEO Series GSE15461. Saccharomyces cerevisiae; Schizosaccharomyces pombe. 9 samples. Type: Expression profiling by array.
Multiple freeze-thaw cycles lead to a loss of consistency in poly(A)-enriched RNA sequencing
GEO Series GSE150097. Homo sapiens. 108 samples. Type: Expression profiling by high throughput sequencing; Other.
Relationship between growth and gene expression in papaya treated with freeze-thaw awakening method
GEO Series GSE130188. Carica papaya. 2 samples. Type: Expression profiling by high throughput sequencing.
Gene expression profile after freeze-thawing at single cell level of Innate Lymphoid Cells (ILCs), Natural Killer (NK) cells , and T cells from human tonsils
GEO Series GSE232673. Homo sapiens. 2 samples. Type: Expression profiling by high throughput sequencing.
Dataset from "A new simulation method of hydrothermal coupling transfer in freeze-thaw process based on the water physical phase change"
<p>The original dataset comprises precipitation and atmospheric temperature monitoring data for the entire duration of 2020-2021 in the region located in Fusong County, Baishan City, southeastern Jilin Province (127°01'-128°06'E, 41°42'-42°49'N, covering an area of 6159 square kilometers)., along with temperature and moisture monitoring data for two designated sites, T01 and T02, at soil depths of 20cm, 40cm, 70cm, 100cm, and 150cm.</p>
Effect of copper ion on recovery process from freeze-thaw injury in Saccharomyces cerevisiae
GEO Series GSE14942. Schizosaccharomyces pombe; Saccharomyces cerevisiae. 6 samples. Type: Expression profiling by array.
Data related to freeze-thaw experiments with bacteria-based healing agent
<p>Open data concerning experimental work. The paper presents the benefits of introducing a bacteria-based healing agent in concrete to enable self-healing, assessed under frost salt scaling conditions. Durability tests such as scaling, water permeability and chloride ingress were performed. In addition, a microstructural analysis based on mercury intrusion porosimetry (MIP), fluorescence microscopy, thin section analysis, scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) spectroscopy was performed. </p>
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Allen Brain Atlas
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.