Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
207
datasets available to search
ShareScore release 0.7.1
Dataset results
207 results for “Huntingtin”
HMGB1 and iVHH4 interaction analyses with huntingtin by pull-down - 15th January 2018
<p>Huntingtin structure function open lab notebook project. </p> <p>Funding Acknowledgment: The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.</p> <p> </p>
Dataset- Unraveling the Molecular Complexity of N-Terminus Huntingtin Oligomers: Insights into Polymorphic Structures
<p>This dataset contains self-assembly simulations of oligomeric huntingtin with varying polyQ lengths, related to "Unraveling the Molecular Complexity of N-Terminus Huntingtin Oligomers: Insights into Polymorphic Structures". Peptide systems are of N17+ polyQ, with polyQ adopting lengths between 7 to 45 repeats.</p> <h1></h1>
Huntingtin Exon 1 Q16 and Q46 pET32a thioredoxin tagged expression and purification 2018/04/09
<p>Huntingtin structure-function open lab notebook project. Huntingtin Exon 1 Q16 and Q46 pET32a thioredoxin tagged expression and purification 2018/04/09.</p>
Huntingtin nanobody purification using osmotic shock cell lysis and growth in M9 media – 2018/04/09
<p>Huntingtin structure-function open lab notebook project. Huntingtin nanobody purification using osmotic shock cell lysis and growth in M9 media – 2018/04/09</p>
Huntingtin nanobody purification using osmotic shock cell lysis – 2018/04/02
<p>Huntingtin structure-function open lab notebook project. Huntingtin nanobody purification using osmotic shock cell lysis – 2018/04/02.</p>
Huntingtin construct design for BioID 2018/04/02
<p>Huntingtin structure-function open lab notebook project. Huntingtin construct design for BioID 2018/04/02.</p>
Huntingtin Exon 1 Q16 and Q46 pET32a thioredoxin tagged expression and purification 2018/04/02
<p>Huntingtin structure-function open lab notebook project. Huntingtin Exon 1 Q16 and Q46 pET32a thioredoxin tagged, attempted expression and purification 2018/04/02</p>
Full-length huntingtin constructs for eukaryotic expression of huntingtin protein with different polyQ lengths 2018/05/01
<p>Huntingtin structure-function open lab notebook project: Full-length huntingtin constructs for eukaryotic expression of huntingtin protein with different polyQ lengths 2018/05/01</p>
Full-length huntingtin Q19, Q23, Q42 and Q54 expression and purification – 2018/04/23
<p>Huntingtin structure-function open lab notebook project. Full-length huntingtin Q19, Q23, Q42 and Q54 expression and purification – 2018/04/23.</p>
Small scale test-expression of full-length huntingtin Q23 with HAP40 in baculoviral expression system production in sf9 insect cells – 2018/05/14
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Small scale test-expression of full-length huntingtin Q23 with HAP40 in baculoviral expression system production in sf9 insect cells – 2018/05/14. To validate our insect cell production system for the generation of physiologically relevant huntingtin constructs, HTT and HAP40 will be co-expressed and purified from sf9 culture. </p>
Differential static light scattering (DSLS) of full-length huntingtin samples of different polyQ lengths (Q19, Q23, Q42 and Q54) – 2018/05/09
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment</strong> - Differential static light scattering (DSLS) of full-length huntingtin samples of different polyQ lengths (Q19, Q23, Q42 and Q54) – 2018/05/09. To investigate how increased polyQ length of the huntingtin protein affects its biophysical properties. </p> <p> </p>
Large scale expression and purification of full-length huntingtin Q23 with and without HAP40 from baculoviral expression system production in sf9 insect cells – 2018/06/04
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Purified huntingtin samples with and without stabilising binding partners (e.g. HAP40) are required for use in structural and functional studies, in particular SAXS experiments. </p>
Large scale expression and purification of full-length huntingtin Q23 with HAP40 from baculoviral expression system production in sf9 insect cells – 2018/05/28
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Purified huntingtin samples with stabilising binding partners (e.g. HAP40) are required for use in structural and functional studies, in particular SAXS experiments. </p>
Construct design for the crystallisation of huntingtin fragments - 2018/06/11
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Objective </strong>- Use the cryo-EM structure of huntingtin in complex with HAP40 <a href="http://www.rcsb.org/structure/6EZ8">http://www.rcsb.org/structure/6EZ8</a> to guide construct design of discrete domains and fragments which could be amenable to successful expression and purification of monodisperse protein samples and subsequent structure solution by X-ray crystallography. </p> <p> </p>
Large scale expression and purification of full-length huntingtin Q19 and Q23 from baculoviral expression system production in sf9 insect cells – 2018/06/18
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Purified huntingtin samples are required for use in structural and functional studies. </p>
Large scale expression and purification of full-length huntingtin Q23 and Q54 from baculoviral expression system production in sf9 insect cells – 2018/07/06
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Purified huntingtin samples are required for use in structural and functional studies. </p>
Attempted expression and purification of full-length huntingtin Q23 with putative interaction partners from baculoviral expression system production in sf9 insect cells – 2018/07/19
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Rationale: </strong>To obtain monodisperse and conformationally constrained huntingtin protein samples for high resolution structural biology, interaction partners are required as highlighted by <a href="https://www.nature.com/articles/nature25502">Guo et al (2018)</a>.</p>
Large scale expression and purification of full-length huntingtin Q23 with HAP40 from baculoviral expression system production in sf9 insect cells – 2018/07/11
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Purified huntingtin samples are required for use in structural and functional studies. </p>
Large scale expression and purification of full-length huntingtin Q42 from baculoviral expression system production in sf9 insect cells – 2018/07/10
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Purified huntingtin samples are required for use in structural and functional studies. </p>
Large scale expression and purification of huntingtin fragment A667-C3140 from baculoviral expression system production in sf9 insect cells – 2018/08/02
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Rationale: </strong>To obtain monodisperse and conformationally constrained huntingtin protein samples for high resolution structural biology.</p>
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.