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325 results for “Purification”
USP5 Zf-UBD Purification & Fluorescence Polarization Displacement Assay Development
<p>USP5 zinc-finger ubiquitin binding domain (Zf-UBD) expression and purification from <em>E. coli, </em>and screening conditions for a fluorescence polarization (FP) displacement assay with a FITC-labelled ubiquitin peptide. </p>
Purification of ACVR1 for co-crystallisation with LDN-193189 and other compounds
<p>Purification of constitutively active ACVR1 for co-crystallisation with LDN-193189 and three other compounds.</p>
Huntingtin Exon 1 Q16 and Q46 pET32a thioredoxin tagged expression and purification 2018/04/09
<p>Huntingtin structure-function open lab notebook project. Huntingtin Exon 1 Q16 and Q46 pET32a thioredoxin tagged expression and purification 2018/04/09.</p>
Huntingtin nanobody purification using osmotic shock cell lysis and growth in M9 media – 2018/04/09
<p>Huntingtin structure-function open lab notebook project. Huntingtin nanobody purification using osmotic shock cell lysis and growth in M9 media – 2018/04/09</p>
Huntingtin nanobody purification using osmotic shock cell lysis – 2018/04/02
<p>Huntingtin structure-function open lab notebook project. Huntingtin nanobody purification using osmotic shock cell lysis – 2018/04/02.</p>
Huntingtin Exon 1 Q16 and Q46 pET32a thioredoxin tagged expression and purification 2018/04/02
<p>Huntingtin structure-function open lab notebook project. Huntingtin Exon 1 Q16 and Q46 pET32a thioredoxin tagged, attempted expression and purification 2018/04/02</p>
Full-length huntingtin Q19, Q23, Q42 and Q54 expression and purification – 2018/04/23
<p>Huntingtin structure-function open lab notebook project. Full-length huntingtin Q19, Q23, Q42 and Q54 expression and purification – 2018/04/23.</p>
Large-scale full-length HTT Q19 and Q42 purification from HEK293T – 2018/04/16
<p>Huntingtin structure-function open lab notebook project. Large-scale full-length HTT Q19 and Q42 purification from HEK293T – 2018/04/16.</p>
Large-scale full-length HTT Q23 purification from HEK293T – 2018/04/30
<p>Huntingtin structure-function open lab notebook project. Large-scale full-length HTT Q23 purification from HEK293T – 2018/04/30.</p>
5-fluoro-tryptophan USP5 Zf-UBD Growth & Purification for 19F NMR Screening
<p>The expression and purification of USP5<sup>171-290</sup> in media supplemented with 5-fluoro-tryptophan. Mass spectrometry, differential scanning fluorimetry and fluorescence polarization assays were used to verify label incorporation, protein folding and ubiquitin peptide binding respectively. Preliminary 19F NMR experiments were completed with the purified protein to determine if there are perturbations in one of the 19F resonances with a ubiquitin peptide, LRLRGG. </p> <p> </p>
Large scale expression and purification of full-length huntingtin Q23 with and without HAP40 from baculoviral expression system production in sf9 insect cells – 2018/06/04
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Purified huntingtin samples with and without stabilising binding partners (e.g. HAP40) are required for use in structural and functional studies, in particular SAXS experiments. </p>
Large scale expression and purification of full-length huntingtin Q23 with HAP40 from baculoviral expression system production in sf9 insect cells – 2018/05/28
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Purified huntingtin samples with stabilising binding partners (e.g. HAP40) are required for use in structural and functional studies, in particular SAXS experiments. </p>
Large scale expression and purification of full-length huntingtin Q19 and Q23 from baculoviral expression system production in sf9 insect cells – 2018/06/18
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Purified huntingtin samples are required for use in structural and functional studies. </p>
Large scale expression and purification of full-length huntingtin Q23 and Q54 from baculoviral expression system production in sf9 insect cells – 2018/07/06
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Purified huntingtin samples are required for use in structural and functional studies. </p>
Attempted expression and purification of full-length huntingtin Q23 with putative interaction partners from baculoviral expression system production in sf9 insect cells – 2018/07/19
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Rationale: </strong>To obtain monodisperse and conformationally constrained huntingtin protein samples for high resolution structural biology, interaction partners are required as highlighted by <a href="https://www.nature.com/articles/nature25502">Guo et al (2018)</a>.</p>
Large scale expression and purification of full-length huntingtin Q23 with HAP40 from baculoviral expression system production in sf9 insect cells – 2018/07/11
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Purified huntingtin samples are required for use in structural and functional studies. </p>
Large scale expression and purification of full-length huntingtin Q42 from baculoviral expression system production in sf9 insect cells – 2018/07/10
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Purified huntingtin samples are required for use in structural and functional studies. </p>
Large scale expression and purification of huntingtin fragment A667-C3140 from baculoviral expression system production in sf9 insect cells – 2018/08/02
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Rationale: </strong>To obtain monodisperse and conformationally constrained huntingtin protein samples for high resolution structural biology.</p>
USP5 Expression and Purification & Development of a Mass Spectrometry USP5 Catalytic Activity Assay
<p>Expression and purification of full length USP5<sup>1-835</sup> for optimization of a mass spectrometry (MS) based assay to detect polyubiquitin cleavage</p>
Optimisation of HTT-HAP40 purification using heparin affinity chromatography (2019/01/14)
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment</strong> - Optimisation of HTT-HAP40 purification using heparin affinity chromatography.</p> <p><strong>Aims</strong> - The current protocol for HTT and HTT-HAP40 purification I am using requires a long incubation of clarified cell lysate with FLAG resin. To potentially improve yields and sample quality, it would perhaps be beneficial to have a quick heparin resin purification step prior to FLAG binding which may also remove contaminating nucleic acid material. To test this hypothesis, small-scale purification of Q23 HTT-HAP40 samples in different buffer systems will be carried out using heparin and FLAG affinity chromatography. </p>
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.