Find research datasets worth reusing

opencc-zeroDec 2020View details →

Migration trajectories of the diamondback moth Plutella xylostella in China inferred from population genomic variation

BACKGROUND: The diamondback moth (DBM), Plutella xylostella (Lepidoptera: Plutellidae), is a notorious pest of cruciferous plants. In temperate areas, annual populations of DBM originate from adult migrants. However, the source populations and migration trajectories of immigrants remain unclear. Here, we investigated migration trajectories of DBM in China with genome-wide single nucleotide polymorphisms (SNPs) genotyped using double-digest RAD (ddRAD) sequencing. We first analyzed patterns of spatial and temporal genetic structure among southern source and northern recipient populations, then inferred migration trajectories into northern regions using discriminant analysis of principal components (DAPC), assignment tests and spatial kinship patterns. RESULTS: Temporal genetic differentiation among populations was low, indicating sources of recipient populations and migration trajectories are stable. Spatial genetic structure indicated three genetic clusters in the southern source populations. Assignment tests linked northern populations to the Sichuan cluster, and central-eastern populations to the South and Yunnan clusters, indicating that Sichuan populations are sources of northern immigrants and South and Yunnan populations are sources of central-eastern populations. First-order (full-sib) and second-order (half-sib) kin pairs were always found within populations, but about 35-40% of third-order (cousin) pairs were found in different populations. Closely related individuals in different populations were in about 35-40% of cases found at distances of 900 to 1500 km, while some were separated by over 2000 km. CONCLUSION: This study unravels seasonal migration patterns in the DBM. We demonstrate how careful sampling and population genomic analyses can be combined to help understand cryptic migration patterns in insects.

opencc-zeroOct 2023View details →

Data from: Fecundity of diamondback moth females when offered honey with pyridalyl, a selective insecticide

We previously reported that the presence of pyridalyl, a selective insecticide, in aqueous honey (50 % v/v) negatively affects the survival of diamondback moth (Plutella xylostella: DBM) adults. However, it remains unclear whether toxicity of pyridalyl in aqueous honey affects the fecundity of adult female DBMs. We analyzed the survival and fecundity of adult DBM under the water, honey, or honey + pyridalyl conditions in glass tubes containing Japanese mustard spinach (Brassica rapa: Komatsuna) leaves for 10 days. The survival of adults under the honey (50 % v/v) and honey + pyridalyl (100-fold dilution) conditions was significantly higher and lower, respectively, than under the water condition during the experimental period. The number of eggs laid by DBM females each day (fecundity) under the water condition was significantly lower than that under the honey condition throughout the experimental period, except on day 1. In contrast, the numbers under the water, and honey + pyridalyl conditions were not significantly different, except on day 2 (the honey + pyridalyl condition was significantly lower). To study the effects of plants grown in pots on the survival and fecundity of DBM females, we conducted experiments using acrylic cages containing potted komatsuna plants. The survival trends under the honey, and honey + pyridalyl conditions in the cages were similar to those in the glass tubes. Fecundity was evaluated based on the total number of subsequent generations (DBM larvae and pupae) on day 10. The numbers were significantly higher in the honey condition than in the water condition. In contrast, the number in the honey + pyridalyl condition was not significantly different from that in the water condition. Based on these findings, the possible use of pyridalyl in honey for the biological control of DBM is discussed.

zenodo36/100

Resolution, validation and divergence of heterozygous haplotypes from pooled long read sequencing of the diamondback moth (Lepidoptera: Plutellidae)

This data sets includes the full set of intermediate genome assemblies produced during our analyses. We make these available for researchers who may be interested in the variation between assembly results and variation within the study organism (Plutella xylostella) prior to removal during subsequent genome processing.

opencc-by-4.0Dec 2020View details →

publicAug 2023View details →

Migration trajectories of the diamondback moth Plutella xylostella in China inferred from population genomic variation

Open the record for dataset details and reuse information.

publicJun 2025View details →

Can parasitized diamondback moth larvae avoid ant attacks?

Open the record for dataset details and reuse information.

publicOct 2023View details →

Data from: Fecundity of diamondback moth females when offered honey with pyridalyl, a selective insecticide

Open the record for dataset details and reuse information.

dryad32/100

Targeting diamondback moth in greenhouses by attracting specific native parasitoids with herbivory-induced plant volatiles

We investigated whether recruitment of specific parasitoids using a specific blend of synthetic herbivory-induced plant volatiles (HIPVs) could be a novel method of pest control in greenhouses. In the Miyama rural area in Kyoto, Japan,diamondback moth (DBM) (Plutella xylostella) larvae are an important pest of cruciferous crops in greenhouses, and Cotesia vestalis, a larval parasitoid of DBM, are found in the surrounding areas. Dispensers of HIPVs that attracted C. vestalis and honey feeders were set inside greenhouses (treated greenhouses). The monthly incidence of DBM in the treated greenhouses was significantly lower than that in the untreated greenhouses across a two-year period. The monthly incidences of C. vestalis and DBM were not significantly different in the untreated greenhouses, whereas the monthly incidence of C. vestalis was significantly higher than that of DBM in the treated greenhouses. Poisson regression analyses showed that, in both years, a significantly higher number of C. vestalis was recorded in the treated greenhouses than in the untreated greenhouses when the number of DBM adults increased. We concluded that DBM was suppressed more effectively by C. vestalis in the treated greenhouses than in the untreated greenhouses.

opencc-zeroNov 2020View details →

dryad32/100

Effect of age on testis development in diamondback moth

Open the record for dataset details and reuse information.

publicApr 2021View details →

dryad32/100

Targeting diamondback moth in greenhouses by attracting specific native parasitoids with herbivory-induced plant volatiles

Open the record for dataset details and reuse information.

publicNov 2020View details →

dryad28/100

Data from: Temperature-sensitive fitness cost of insecticide resistance in Chinese populations of the diamondback moth Plutella xylostella

Alleles conferring a higher adaptive value in one environment may have a detrimental impact on fitness in another environment. Alleles conferring resistance to pesticides and drugs provide textbook examples of this trade-off as, in addition to conferring resistance to these molecules, they frequently decrease fitness in pesticide/drug-free environments. We show here that resistance to chlorpyrifos, an organophosphate (OP), in Chinese populations of the diamondback moth, Plutella xylostella, is conferred by two mutations of ace1 – the gene encoding the acetylcholinesterase enzyme targeted by OPs – affecting the amino acid sequence of the corresponding protein. These mutations were always linked, consistent with the segregation of a single resistance allele, ace1R, carrying both mutations, in the populations studied. We monitored the frequency of ace1R (by genotyping more than 20 000 adults) and the level of resistance (through bioassays on more than 50 000 individuals) over several generations. We found that the ace1R resistance allele was costly in the absence of insecticide and that this cost was likely recessive. This fitness costs involved a decrease in fecundity: females from resistant strains laid 20% fewer eggs, on average, than females from susceptible strains. Finally, we found that the fitness costs associated with the ace1R allele were greater at high temperatures. At least two life history traits were involved: longevity and fecundity. The relative longevity of resistant individuals was affected only at high temperatures and the relative fecundity of resistant females – which was already affected at temperatures optimal for development – decreased further at high temperatures. The implications of these findings for resistance management are discussed.

opencc-zeroDec 2014View details →

opencc-by-4.0Feb 2022View details →

Characterization of Molting Process during the Different De-velopmental Stages of the Diamondback Moth Plutella xy-lostella

Supplementary Materials:  Figure S1: The pre-ecdysis behavior of the larval-larval ecdysis of P. xylostella larva; Figure S2: The “peeled” prepupa of P. xylostella and the “eclosion hole” of on P. xylostella cocoon; Video S1: The hatching process of P. xylostella ; Video S2: The pre-ecdysis I behavior of larval-larval ecdysis of P. xylostella; Video S3: The pre-ecdysis II behavior of larval-larval ecdysis of P. xylostella; Video S4: The ecdysis behavior of larval-larval ecdysis of P. xylostella; Video S5: The cocooning behavior of larval-pupal metamorphosis of P. xylostella; Video S6: The pre-ecdysis I behavior of larval-pupal metamorphosis of P. xylostella; Video S7: The pre-ecdysis II behavior of larval-pupal metamorphosis of P. xylostella; Video S8: The ecdysis behavior of larval-pupal metamorphosis of P. xylostella; Video S9: The pre-eclosion behavior of P. xylostella pupa; Video S10: The intersegmental membrane of the abdomen of P. xylostella pupa inflates before eclosion; Video S11: The eclosion behavior of P. xylostella; Video S12: The stage II of the post-eclosion behavior of P. xylostella adult; Video S13: The stage III of the post-eclosion behavior of P. xylostella adult; Video S14: The stage IV of the post-eclosion behavior of P. xylostella adult.

Figures 3-16 from: Landry J, Hebert P (2013) Plutella australiana (Lepidoptera, Plutellidae), an overlooked diamondback moth revealed by DNA barcodes. ZooKeys 327: 43-63. https://doi.org/10.3897/zookeys.327.5831

Figures 3-16 - Dorsal aspect of forewings of Plutella australiana and Plutella xylostella. SpecimenID (sample ID) in parentheses. Scale bar = 2 mm. 3 Plutella australiana, male holotype, Australia: Canberra (BIOUG00844-C06) 4 Plutella australiana, female paratype, Australia: New South Wales, Bimbi State Forest (AM 2272) 5 Plutella australiana, male paratype, Australia: Canberra (BIOUG00788-F11) 6 Plutella australiana, female, Australia: Canberra (BIOUG02123-E08) 7 Plutella australiana, female, Australia: Canberra (BIOUG02112-F11) 8 Plutella australiana, female, Australia: Canberra (BIOUG02108-C09) 9 Plutella australiana, male, Australia: Canberra (CCDB-15830-E08) 10 Plutella xylostella, male, Australia: Canberra (CCDB-12828-E05) 11 Plutella xylostella, male, Australia: Canberra (BIOUG02113-F05) 12 Plutella xylostella, female, Australia: Canberra (BIOUG01172-A09) 13 Plutella xylostella, female, Australia: Canberra (CCDB-12828-G07) 14 Plutella xylostella, female, Australia: Canberra (CCDB-12828-H01) 15 Plutella xylostella, female, Canada: Québec (CNCLEP00098486) 16 Plutella xylostella, female holotype of Plutella karsholtella, Canary Islands: Tenerife (ZMUC00401145).

Figures 23-28 from: Landry J, Hebert P (2013) Plutella australiana (Lepidoptera, Plutellidae), an overlooked diamondback moth revealed by DNA barcodes. ZooKeys 327: 43-63. https://doi.org/10.3897/zookeys.327.5831

Figures 23-28 - Male genitalia of Plutella australiana and Plutella xylostella. 23–24, valva, inner aspect 23 Plutella australiana (slide JFL1689, specimen 08-NSWHH-1277) 24 Plutella xylostella (slide JFL1733, specimen BIOUG02248-G02). 25–26, phallus, dorsal aspect 25 Plutella australiana (slide JFL1690, specimen 08-NSWHH-1340) 26 Plutella xylostella (slide JFL1688, specimen 06-TASB-01769). 27–28, genitalia with phallus removed, ventral aspect 27 Plutella australiana (slide JFL1735, specimen BIOUG00987-E12) 28 Plutella xylostella (slide JFL1734, specimen 09-NSWHH-1674). Scale bar = 100µ; all to same scale. at = anal tube; scl = sacculus; tp = teguminal process; zss = zone of spiniform setae.

Figures 29-32 from: Landry J, Hebert P (2013) Plutella australiana (Lepidoptera, Plutellidae), an overlooked diamondback moth revealed by DNA barcodes. ZooKeys 327: 43-63. https://doi.org/10.3897/zookeys.327.5831

Figures 29-32 - Female genitalia. 29 Plutella australiana, ventral aspect (slide JFL1684, specimen 07-NSWBB-0046) 30 Plutella xylostella, ditto (slide JFL1685, specimen 07-NSWBB-0144) 31 Plutella australiana, lateral aspect of antrum projection (slide JFL1736, specimen BIOUG00788-G06) 32 Plutella xylostella, ditto (slide MIC6811, specimen BIOUG02113-D10). Scale: Figs 29–30 = 200µ; Figs 31–32 = 100µ.

Figures 17-22 from: Landry J, Hebert P (2013) Plutella australiana (Lepidoptera, Plutellidae), an overlooked diamondback moth revealed by DNA barcodes. ZooKeys 327: 43-63. https://doi.org/10.3897/zookeys.327.5831

Figures 17-22 - Male genitalia of Plutella australiana and Plutella xylostella. 17–18 tegumen-gnathos, ventral aspect 17 Plutella australiana (slide JFL1730, specimen BIOUG00788-F11) 18 Plutella xylostella (slide JFL1729, specimen BIOUG02113-A06). 19–20, tegumen-uncus-gnathos-vinculum complex, lateral aspect, valvae and phallus removed 19 Plutella australiana (slide JFL1732, specimen BIOUG02248-G03) 20 Plutella xylostella (slide JFL1733, specimen BIOUG02248-G02). 21–22 vinculum–saccus, ventral aspect 21 Plutella australiana (slide JFL1730) 22 Plutella xylostella (slide JFL1729). Scale bar = 100µ; all to same scale. at = anal tube; gn = gnathos; sac = saccus; teg = tegumen; tp = teguminal process; vin = vinculum.

Figure 2 from: Landry J, Hebert P (2013) Plutella australiana (Lepidoptera, Plutellidae), an overlooked diamondback moth revealed by DNA barcodes. ZooKeys 327: 43-63. https://doi.org/10.3897/zookeys.327.5831

Figure 2 - Sites in Australia where specimens of Plutella xylostella (red) and Plutella australiana (blue) have been collected. The pie diagrams show the proportion of the two species at each site. These records only include specimens identified through DNA barcode analysis.

Figure 1 from: Landry J, Hebert P (2013) Plutella australiana (Lepidoptera, Plutellidae), an overlooked diamondback moth revealed by DNA barcodes. ZooKeys 327: 43-63. https://doi.org/10.3897/zookeys.327.5831

Figure 1 - NJ tree based on K2P distances for the barcode region of the cytochrome c oxidase I gene among seven species of the genus Plutella and one member of the closely allied genus Eidophasia. Because of the large number of specimens for both Plutella xylostella and Plutella australiana, the tree nodes have been collapsed and specimen records are plotted by state for Australia and by country of origin in other cases. The bracketed numerals indicate the number of specimens from each site. The type specimen of Plutella karsholtella is reassigned to Plutella xylostella and is the only specimen of this species from Spain (Canary Islands).