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Multispectral absorbance and fluorescence analysis of dissolved organic carbon in water samples taken from the Upper Clark Fork River (Montana, USA) during water years 2017 and 2018 (1 Oct 2016 - 30 Sep 2018)
The Upper Clark Fork River (UCFR) Long Term Research in Environmental Biology (LTREB) umbrella monitoring project generating these data is conducted separately and complementarily to the 200-million-dollar (USD) superfund project for ecological restoration of the UCFR, associated tributaries, and head water streams including Silver Bow and Warm Springs Creeks. Restoration along the UCFR in western Montana includes removal of metal-laden floodplain soils, lowering of the floodplain to its original elevation, and re-vegetation of over 70 km of the river’s floodplain closest to contaminant sources. The UCFR LTREB project includes bi-weekly water quality monitoring across the first 200 km of the river and its major tributaries along a gradient of heavy metal contamination associated with historic mining. Monitoring includes inorganic phosphorus and nitrogen concentrations, biotic standing stocks, and dissolved and whole-water heavy metal concentrations. The monitoring program began in 2017 with funding likely to be extended through 2028. The original analytical intent for these data was to assess the response of river dissolved organic carbon to the floodplain restoration. Data are multispectral absorbance and fluorescence analyses of organic carbon dissolved in samples of well-mixed river thalweg water. Data include excitation-emission matrices, absorbance spectroscopy, as well as absorbance and fluorometric summary indices calculated at specific wavelengths of excitation and emission. Data are from the 2017 and 2018 water years (1 Oct 2016 to 30 Sep 2018). Data were collected on the Upper Clark Fork River (USGS HUC 17010201) at 13 project sites distributed along the river from the vicinity of Anaconda to Missoula, Montana, USA. These data are a correction of a previously published data product (doi:10.6073/pasta/6ba30f4ebb63175a4399c5d0aa6a8698). Inconsistencies between availability of EEMS data, absorbance data, and fluorometric summary metrics have been corrected. P
High-frequency dissolved oxygen, water temperature, wind speed, and radiation data; stream and in-lake nutrient concentration data; and daily metabolism and nutrient loading estimates for 16 lakes in North America and Northern Europe.
In lakes, ecosystem structure and processes are influenced by gross primary production (GPP), ecosystem respiration (R), and net ecosystem production (NEP). The rates of these metabolic processes are often controlled by resource availability, which often reflects catchment loads. Although the relationship between catchment loads and in-lake nutrient concentrations may be well defined in specific lakes, we explored how watershed vs. in-lake predictors of metabolism compare across lake types. To do this, we combined stream loads of carbon (C), nitrogen (N), and phosphorus (P) with high frequency in situ monitoring of lake metabolism and in-lake C, N, and P concentrations from 16 lakes spanning a range of latitudes (39 to 64 degrees N), inflowing stream (0 - 6 streams), and trophic status (oligotrophic to eutrophic). The data package includes high-frequency dissolved oxygen, water temperature, wind speed, and solar radiation data as well as daily estimates of GPP, R, and NEP derived from those data. In addition, the data package includes in-lake and stream concentrations of dissolved organic carbon, total nitrogen, and total phosphorus and stream discharge data. The package also includes estimates of daily carbon, nitrogen and phosphorus loading to each lake derived from the stream concentrations and discharge.
Temperature and concentration of dissolved oxygen in river water measured in the Upper Clark Fork River (Montana, USA) during 2020 and 2021
The LTREB (Long Term Research in Environmental Biology) monitoring project is a portion of the $200 million-dollar (USD) superfund project for ecological restoration of the Upper Clark Fork River (UCFR), associated tributaries, and head water streams including Silver Bow and Warm Springs Creeks. Restoration along the Upper Clark Fork River includes removal of metal-laden floodplain soils, lowering of the floodplain to its original elevation, and re-vegetation of over 70 km of the river's floodplain closest to contaminant sources. The UCFR Long Term Research in Environmental Biology (LTREB) project includes bi-weekly water quality monitoring across a 200-km gradient of heavy metal contamination associated with historic mining. Monitoring includes inorganic phosphorus and nitrogen concentrations, biotic standing stocks, and dissolved and whole-water heavy metal concentrations. The UCFR LTREB monitoring project is conducted within the first 200km of the UCFR and associated tributaries located in western Montana. The monitoring program began in 2017 and will be completed in the year 2023 with potential for funding extension. Surface water samples represented in this data product are collected from six sites on the mainstem of the UCFR. River water is measured at each monitoring site using miniDOT Loggers. Dissolved oxygen (DO) and Temperature (T) are recorded by the sensor at five-, 10-, or 15-minute intervals (as found in the raw data files), then interpolated as needed to five-minute intervals in the product data tables. The analysis-ready data of this dataset represent Quality Assurance and Quality Control (QAQC) -processed DO concentrations from six sites on the mainstem of the UCFR collected in 2020 and 2021.
Multispectral absorbance and fluorescence analysis of dissolved organic carbon in water samples taken from the Upper Clark Fork River (Montana, USA) during water year 2019 (1 Oct 2018 - 30 Sep 2019)
The Upper Clark Fork River (UCFR) Long Term Research in Environmental Biology (LTREB) umbrella monitoring project generating these data is conducted separately and complementarily to the 200-million-dollar (USD) superfund project for ecological restoration of the UCFR, associated tributaries, and head water streams including Silver Bow and Warm Springs Creeks. Restoration along the UCFR in western Montana includes removal of metal-laden floodplain soils, lowering of the floodplain to its original elevation, and re-vegetation of over 70 km of the river’s floodplain closest to contaminant sources. The UCFR LTREB project includes bi-weekly water quality monitoring across the first 200 km of the river and its major tributaries along a gradient of heavy metal contamination associated with historic mining. Monitoring includes inorganic phosphorus and nitrogen concentrations, biotic standing stocks, and dissolved and whole-water heavy metal concentrations. The monitoring program began in 2017 with funding likely to be extended through 2028. The original analytical intent for these data was to assess the response of river dissolved organic carbon to the floodplain restoration. Data are multispectral absorbance and fluorescence analyses of organic carbon dissolved in samples of well-mixed river thalweg water. Data include excitation-emission matrices, absorbance spectroscopy, as well as absorbance and fluorometric summary indices calculated at specific wavelengths of excitation and emission. Data are from the 2019 water year (1 Oct 2018 to 30 Sep 2019). Data were collected on the Upper Clark Fork River (USGS HUC 17010201) at 13 project sites distributed along the river from the vicinity of Anaconda to Missoula, Montana, USA.
Secchi depth data and discrete depth profiles of water temperature, dissolved oxygen, conductivity, specific conductance, photosynthetic active radiation, oxidation-reduction potential, and pH for Beaverdam Reservoir, Carvins Cove Reservoir, Falling Creek Reservoir, Gatewood Reservoir, and Spring Hollow Reservoir in southwestern Virginia, USA 2013-2025
Discrete depth profiles of water temperature, dissolved oxygen, oxidation-reduction potential, conductivity, specific conductance, and pH were collected with multiple handheld water quality probes and discrete depth profiles of photosynthetically active radiation (PAR) were collected with a LI-COR underwater light meter from 2013 to 2025 in five drinking water reservoirs in southwestern Virginia, USA. These reservoirs are: Beaverdam Reservoir (Vinton, Virginia), Carvins Cove Reservoir (Roanoke, Virginia), Falling Creek Reservoir (Vinton, Virginia), Gatewood Reservoir (Pulaski, Virginia), and Spring Hollow Reservoir (Salem, Virginia). Beaverdam, Carvins Cove, Falling Creek, and Spring Hollow Reservoirs are owned and operated by the Western Virginia Water Authority as primary or secondary drinking water sources for Roanoke, Virginia, and Gatewood Reservoir is a drinking water source for the Town of Pulaski, Virginia. All discrete depth profiles were collected on approximately 1-meter intervals. The data package consists of two datasets: 1) Secchi depth data; and 2) discrete depth profiles of multiple water quality variables measured by handheld sensors. The Secchi data and discrete depth profiles were measured at the deepest site of each reservoir adjacent to the dam, as well as other in-reservoir sites. Handheld sensor measurements were also collected at a gauged weir on the primary inflow tributary, other inflows, and outflows at Falling Creek Reservoir; inflows and outflows at Beaverdam Reservoir; and inflows at Carvins Cove Reservoir. In 2021, YSI handheld data were also collected from a littoral site in Beaverdam Reservoir. In 2025, YSI handheld data were collected monthly from June to October from nine littoral sites around the perimeter of Falling Creek Reservoir. From 2024 - 2025, additional within-reservoir depth profiles were collected in Carvins Cove Reservoir and multiple sites. Data were collected approximately fortnightly in the spring months (March - Ma
Time series of high-frequency profiles of depth, temperature, dissolved oxygen, conductivity, specific conductance, chlorophyll a, turbidity, pH, oxidation-reduction potential, photosynthetically active radiation, colored dissolved organic matter, phycocyanin, phycoerythrin, and descent rate for Beaverdam Reservoir, Carvins Cove Reservoir, Falling Creek Reservoir, Gatewood Reservoir, and Spring Hollow Reservoir in southwestern Virginia, USA 2013-2025
Depth profiles of water biogeochemical properties were collected with SeaBird Electronics (SBE) Conductivity, Temperature, and Depth (CTD) profilers from 2013-2025 at five drinking water reservoirs in southwestern Virginia, USA. The study reservoirs are: Beaverdam Reservoir (Vinton, Virginia), Carvins Cove Reservoir (Roanoke, Virginia), Falling Creek Reservoir (Vinton, Virginia), Gatewood Reservoir (Pulaski, Virginia), and Spring Hollow Reservoir (Salem, Virginia). Beaverdam, Carvins Cove, Falling Creek, and Spring Hollow Reservoirs are owned and operated by the Western Virginia Water Authority as primary or secondary drinking water sources for Roanoke, Virginia, and Gatewood Reservoir is a drinking water source for the town of Pulaski, Virginia. The dataset consists of CTD depth profiles measured at the deepest site of each reservoir adjacent to the dam as well as other upstream reservoir sites. The profiles were collected approximately fortnightly in the spring months, weekly in the summer and early autumn, and monthly in the late autumn and winter. Beaverdam Reservoir, Carvins Cove Reservoir, and Falling Creek Reservoir were sampled every year in the dataset (2013-2025); Spring Hollow Reservoir was only sampled 2013-2017 and 2019; and Gatewood Reservoir was only sampled in 2016. Data availability differs across years due to additional sensors that have been added or replaced over time. From 2013-2016, profiles were taken with a CTD equipped with an SBE 43 Dissolved Oxygen sensor and an ECO FLNTU sensor for turbidity and chlorophyll. From 2017-2025, profiles were taken with a CTD equipped with an SBE 43 Dissolved Oxygen sensor, an ECO FLNTU sensor for turbidity and chlorophyll, a PAR-LOG ICSW sensor for photosynthetically active radiation, and a SBE 27 pH and ORP (oxidation-reduction potential) sensor. In 2022 and 2023, profiles were also taken with an additional CTD equipped with an SBE 43 Dissolved Oxygen sensor; an ECO Triplet Scattering Fluorescence sensor for
Concentration of dissolved organic carbon in water samples taken from the Upper Clark Fork River (Montana, USA) during water year 2021 (1 Oct 2020 - 30 Sep 2021)
The Upper Clark Fork River (UCFR) Long Term Research in Environmental Biology (LTREB) umbrella monitoring project generating these data is conducted separately and complementarily to the 200-million-dollar (USD) superfund project for ecological restoration of the UCFR, associated tributaries, and head water streams including Silver Bow and Warm Springs Creeks. Restoration along the UCFR in western Montana includes removal of metal-laden floodplain soils, lowering of the floodplain to its original elevation, and re-vegetation of over 70 km of the river’s floodplain closest to contaminant sources. The UCFR LTREB project includes bi-weekly water quality monitoring across the first 200 km of the river and its major tributaries along a gradient of heavy metal contamination associated with historic mining. Monitoring includes inorganic phosphorus and nitrogen concentrations, biotic standing stocks, and dissolved and whole-water heavy metal concentrations. The monitoring program began in 2017 with funding extended through 2028. The original analytical intent for these data was to assess the response of river dissolved organic carbon to the floodplain restoration. Data are Aurora Total Organic Carbon combustion analyses of the concentration of organic carbon dissolved in filtered samples of well-mixed river thalweg water. Data are from the 2021 water year (1 Oct 2020 to 30 Sep 2021) from samples collected on the Upper Clark Fork River (USGS HUC 17010201) at project sites distributed along the river from the vicinity of Anaconda to Missoula, Montana, USA.
Concentration of dissolved organic carbon in water samples taken from the Upper Clark Fork River (Montana, USA) during water year 2023 (1 Oct 2022 - 30 Sep 2023)
The Upper Clark Fork River (UCFR) Long Term Research in Environmental Biology (LTREB) umbrella monitoring project generating these data is conducted separately and complementarily to the 200-million-dollar (USD) superfund project for ecological restoration of the UCFR, associated tributaries, and head water streams including Silver Bow and Warm Springs Creeks. Restoration along the UCFR in western Montana includes removal of metal-laden floodplain soils, lowering of the floodplain to its original elevation, and re-vegetation of over 70 km of the river’s floodplain closest to contaminant sources. The UCFR LTREB project includes bi-weekly water quality monitoring across the first 200 km of the river and its major tributaries along a gradient of heavy metal contamination associated with historic mining. Monitoring includes inorganic phosphorus and nitrogen concentrations, biotic standing stocks, and dissolved and whole-water heavy metal concentrations. The monitoring program began in 2017 with funding extended through 2028. The original analytical intent for these data was to assess the response of river dissolved organic carbon to the floodplain restoration. Data are total organic carbon combustion analyses (Shimadzu Scientific Instruments) of the concentration of organic carbon dissolved in filtered samples of well-mixed river thalweg water. Data are from the 2023 water year (1 Oct 2022 to 30 Sep 2023) from samples collected on the Upper Clark Fork River (USGS HUC 17010201) at project sites distributed along the river from the vicinity of Anaconda to Missoula, Montana, USA.
Concentration of dissolved organic carbon in water samples taken from the Upper Clark Fork River (Montana, USA) during water year 2024 (1 Oct 2023 - 30 Sep 2024)
The Upper Clark Fork River (UCFR) Long Term Research in Environmental Biology (LTREB) umbrella monitoring project generating these data is conducted separately and complementarily to the 200-million-dollar (USD) superfund project for ecological restoration of the UCFR, associated tributaries, and head water streams including Silver Bow and Warm Springs Creeks. Restoration along the UCFR in western Montana includes removal of metal-laden floodplain soils, lowering of the floodplain to its original elevation, and re-vegetation of over 70 km of the river’s floodplain closest to contaminant sources. The UCFR LTREB project includes bi-weekly water quality monitoring across the first 200 km of the river and its major tributaries along a gradient of heavy metal contamination associated with historic mining. Monitoring includes inorganic phosphorus and nitrogen concentrations, biotic standing stocks, and dissolved and whole-water heavy metal concentrations. The monitoring program began in 2017 with funding extended through 2028. The original analytical intent for these data was to assess the response of river dissolved organic carbon to the floodplain restoration. Data are total organic carbon combustion analyses (Shimadzu Scientific Instruments) of the concentration of organic carbon dissolved in filtered samples of well-mixed river thalweg water. Data are from the 2024 water year (1 Oct 2023 to 30 Sep 2024) from samples collected on the Upper Clark Fork River (USGS HUC 17010201) at project sites distributed along the river from the vicinity of Anaconda to Missoula, Montana, USA.
Soil dissolved organic matter and greenhouse gas fluxes from intact Delmarva Bay wetland soil cores during laboratory simulation of groundwater level rise
Wetlands in low-relief landscapes have dynamic terrestrial-aquatic interfaces as surface water and groundwater levels fluctuate seasonally and these variable water levels influence wetland carbon cycling. Seasonal changes in groundwater levels determine which soils are hydrologically connected to the wetland surface water-groundwater continuum and therefore which soils act as carbon sources. To quantify groundwater-mediated soil dissolved organic matter (DOM) and greenhouse gas (CO2 and CH4) fluxes, we performed a laboratory simulation of groundwater rise on intact soil cores. Soil cores were collected from four Delmarva Bay wetlands located in the low-relief landscape of the Delmarva Peninsula in the Mid-Atlantic United States. At each wetland, two cores (length = 60 cm, diameter = 10.2 cm) were collected: one from within the wetland basin and the second from the transitional zone near the edge of the wetland basin (total number of cores collected = 8). To characterize seasonal hydrologic conditions at each wetland site where intact soil cores were collected (e.g., mean water level, number of saturation events, duration of saturation), we used high frequency water level data collected in wetland center and upland groundwater monitoring wells. In the lab, cores were re-saturated with groundwater over 15 days and after cores were fully re-saturated, core water levels were maintained for an additional 25 days. Water levels in each head tank and soil core were manually recorded on sub-daily timesteps. Rhizon soil porewater samplers were installed at 8, 20, and 40 cm below the soil surface reflecting the expected depths of the O, A, and B soil horizons across the wetland sites. Source groundwater, soil porewater, and exfiltrated surface water samples were collected daily from the soil cores and analyzed for pH, ORP, and DOM concentration (dissolved organic carbon) and DOM composition (absorbance and fluorescence metrics). Discrete measurements of CO2 and CH4 fluxes were
Concentration of dissolved organic carbon in water samples taken from the Upper Clark Fork River (Montana, USA) during water year 2022 (1 Oct 2021 - 30 Sep 2022)
The Upper Clark Fork River (UCFR) Long Term Research in Environmental Biology (LTREB) umbrella monitoring project generating these data is conducted separately and complementarily to the 200-million-dollar (USD) superfund project for ecological restoration of the UCFR, associated tributaries, and head water streams including Silver Bow and Warm Springs Creeks. Restoration along the UCFR in western Montana includes removal of metal-laden floodplain soils, lowering of the floodplain to its original elevation, and re-vegetation of over 70 km of the river’s floodplain closest to contaminant sources. The UCFR LTREB project includes bi-weekly water quality monitoring across the first 200 km of the river and its major tributaries along a gradient of heavy metal contamination associated with historic mining. Monitoring includes inorganic phosphorus and nitrogen concentrations, biotic standing stocks, and dissolved and whole-water heavy metal concentrations. The monitoring program began in 2017 with funding extended through 2028. The original analytical intent for these data was to assess the response of river dissolved organic carbon to the floodplain restoration. Data are primarily Aurora Total Organic Carbon combustion analyses of the concentration of organic carbon dissolved in filtered samples of well-mixed river thalweg water. A few samples from the final campaign in the dataset were analyzed with a Shimadzu instrument using a similar method. Data are from the 2022 water year (1 Oct 2021 to 30 Sep 2022) from samples collected on the Upper Clark Fork River (USGS HUC 17010201) at project sites distributed along the river from the vicinity of Anaconda to Missoula, Montana, USA.
Stage height, discharge, water temperature, pH, conductivity, and dissolved oxygen at Cascade Brook in Black Rock Forest, Cornwall, NY 1998 - 2015
Black Rock Forest established a stream monitoring station in the Cascade Brook watershed in 1998. The station is equipped with a 120-degree sharp-crested v-notch weir and was built to continuously monitor the flow, temperature, pH, conductivity, and dissolved oxygen content of the stream water in Cascade Brook in New York. The Cascade Brook watershed encompasses 135 hectares (334 acres), is generally a southern aspect, and has been little affected by human activity in the last century. The forest is deciduous and dominated by oak species. The station records data hourly, and while water chemistry sensors have been used intermittently through the decades, stage height and discharge has been measured consistently from1998 - 2015.
Time series of high-frequency sensor data measuring water temperature, dissolved oxygen, pressure, conductivity, specific conductance, total dissolved solids, chlorophyll a, phycocyanin, fluorescent dissolved organic matter, and turbidity at discrete depths in Falling Creek Reservoir, Virginia, USA in 2018-2025
We monitored water quality in Falling Creek Reservoir (Vinton, Virginia, USA; 37.30325 -79.8373) with high-frequency (10-minute) sensors in 2018-2025. All variables were measured at the deepest site of the reservoir adjacent to the dam. Falling Creek Reservoir is owned and managed by the Western Virginia Water Authority as a primary drinking water source for Roanoke, Virginia. This data product consists of one dataset compiled of depth profiles of water temperature on 1-m intervals from 0.1 to 9 m depth; dissolved oxygen at 5 m and 9 m depth; pressure at 9 m depth; and temperature, dissolved oxygen, conductivity, specific conductance, chlorophyll a, phycocyanin, total dissolved solids, fluorescent dissolved organic matter, turbidity, and pressure at ~1.6 m depth. The dataset is accompanied by a sensor maintenance log and quality assurance/quality control analysis scripts.
Time series of dissolved methane and carbon dioxide concentrations for Falling Creek Reservoir, Beaverdam Reservoir and Carvins Cove Reservoir in southwestern Virginia, USA during 2015-2025
Surface samples and depth profiles of carbon dioxide and methane concentrations were sampled from 2015 to 2025 in three drinking water reservoirs in southwestern Virginia, USA: Beaverdam Reservoir (Vinton, Virginia), Carvins Cove Reservoir (Roanoke, Virginia), and Falling Creek Reservoir (Vinton, Virginia). All three reservoirs are owned and operated by the Western Virginia Water Authority as primary or secondary drinking water sources for Roanoke, Virginia. The dataset consists of depth profiles of dissolved greenhouse gas (carbon dioxide, methane) samples measured at the deepest site of each reservoir adjacent to the dam. Additional surface samples were collected at a gauged weir on Falling Creek Reservoir's primary inflow tributary, from a wetland adjacent to Falling Creek Reservoir, and from the reservoir outflow. At Beaverdam Reservoir, additional samples were collected at three outflow sites below the dam and at the mid-reservoir outflow. Samples were collected approximately fortnightly from March-April, weekly from May-October, and monthly in November-February at Falling Creek Reservoir and Beaverdam Reservoir. Samples were collected twice in 2025 at Carvins Cove Reservoir. Additionally, upstream surface samples were taken at multiple tributaries in Carvins Cove Reservoir. In 2019, surface samples along the stream and reservoir continuum in both Falling Creek Reservoir and Beaverdam Reservoir were collected monthly during the summer stratified period. In 2025, littoral samples were collected from Falling Creek Reservoir monthly from June to October (see site descriptions file for geographic coordinates of sampling sites). A maintenance log and quality assurance/quality control analysis scripts accompany the data package.
Time series of high-frequency sensor data measuring water temperature, dissolved oxygen, conductivity, specific conductance, total dissolved solids, chlorophyll a, phycocyanin, fluorescent dissolved organic matter, and turbidity at discrete depths, and water level in Beaverdam Reservoir, Virginia, USA in 2009-2025
We monitored water level and water quality in Beaverdam Reservoir (Vinton, Virginia, USA; 37.31288, -79.8159) with visual observations and high-frequency (10- to 15-minute resolution) sensors in 2009-2025. All variables were measured at the deepest site of the reservoir adjacent to the dam. Beaverdam Reservoir is owned and managed by the Western Virginia Water Authority as a secondary drinking water source for Roanoke, Virginia. This data package is comprised of three datasets: 1) bvre-waterlevel_2009_2025.csv, 2) bvre-sensorstring_2016_2020.csv, and 3) bvre-waterquality_2020_2025.csv. 1) bvre-waterlevel_2009_2025.csv contains water level observations of the staff gauge at a platform near the reservoir's dam by both the Western Virginia Water Authority and the Virginia Tech Reservoir Group LTREB field crew. This dataset spans 2009 to 2025, with data collection still ongoing. 2) bvre-sensorstring_2016_2020.csv consists of a water temperature profile at ~1-meter intervals from the surface of the reservoir to 10.5 m below the water, complemented by intermittent data collected by a dissolved oxygen logger deployed at 5 m or 10 m. A sonde measuring water temperature, conductivity, specific conductance, chlorophyll a, phycocyanin, total dissolved solids, dissolved oxygen, fluorescent dissolved organic matter, and turbidity was additionally deployed at ~1.5 m depth. This dataset spans 2016 to 2020, with no additional data collection beyond the last observation. The third dataset is bvre-waterquality_2020_2025.csv, with data collection still ongoing and an accompanying maintenance log. This dataset contains: a) a temperature string with 13 temperature sensors deployed ~1 m apart from the surface to 0.5 m above the sediments of the reservoir; b) two dissolved oxygen sensors, one in the middle of the string and one sensor above the sediments; and c) a pressure sensor just above the sediments. The same sonde from the first 2016-2020 dataset is also included in this 2020-2025 d
Composition and biodegradability of dissolved organic matter leached from eroding coastal soils and permafrost in seawater, from Drew Point, Alaska
Eroding permafrost coastlines export significant quantities of organic carbon (OC) to the marine environment, similar in magnitude to riverine particulate OC fluxes to the Arctic Ocean. Moreover, erosion rates are predicted to increase due to warming temperatures, declines in sea ice, and increasing waves. While erosion primarily mobilizes organic matter in the particulate form, this material can be leached to dissolved organic matter (DOM). This DOM may be incorporated by microbial communities and fuel marine food webs or decomposed to form greenhouse gases like carbon dioxide and methane. Many studies show that permafrost-derived organic matter can be rapidly decomposed in soils and freshwater, but few studies examine the fate of permafrost organic matter in seawater. To address this knowledge gap, we designed a laboratory experiment to leach coastal soils and permafrost in seawater and examine the composition and biodegradability of leached DOM. Coastal soil/sediment was cored near Drew Point, Alaska in 2019, representing three horizons found within rapidly eroding permafrost bluffs: seasonally thawed active layer soils, Holocene terrestrial soils and/or lacustrine sediments, and late-Pleistocene relict marine sediments. To measure dissolved organic carbon (DOC) leaching yields, we placed soil/sediments in Beaufort Sea seawater for 24 hours before filtering to remove particulates. To measure biodegradable dissolved organic carbon (BDOC), we conducted an aerobic incubation experiment following the methods suggested by Vonk et al. (2015). Briefly, leachates were incubated at approximately room temperature for 26 and 90 days to measure DOC loss due to remineralization and/or incorporation into microbial biomass. Additionally, we used chromophoric dissolved organic matter (CDOM) measurements and ultra-high resolution mass spectrometry (FT-ICR MS) to examine the initial leachate DOM composition. References: Vonk, J. E., Tank, S. E., Mann, P. J., Spencer, R. G. M., Tre
North Temperate Lakes LTER: High Frequency Dissolved Oxygen Data - Trout Lake Buoy2 - ADCP 2005
During the fall 2005, this instrumented buoy on Trout Lake was equipped with 4 Greenspan dissolved oxygen sensors placed at depths from the lake surface to 10m. Parameters measured at each depth included dissolved oxygen (DO), DO saturation and water temperature. Currently, this buoy measures air temperature, relative humidity and various wind parameters. It also is equipped with a thermistor chain that measures water temperature from depths placed every 0.5-1m from the surface through 13m. An acoustic Doppler current profiler (ADCP) is associated with this buoy. Data are usually collected every 10 minutes with occasional periods of 2 minute data for short periods to answer specific questions. After correcting for flux to or from the atmosphere and vertical mixing within the water column, high frequency measurements of dissolved gases such as carbon dioxide and oxygen can be used to estimate gross primary productivity, respiration, and net ecosystem productivity, the basic components of whole lake metabolism. Dissolved oxygen (DO) data were collected for period 27 Aug 2005 through 3 Nov 2005. Sampling Frequency: varies for instantaneous sample. averaged to hourly and daily values from one minute samples Number of sites: 1
North Temperate Lakes LTER: High Frequency Meteorological and Dissolved Oxygen Data - Sparkling Bog North Buoy 2008 - 2012
The instrumented buoy on Sparkling Bog North is equipped with a dissolved oxygen sensor, a thermistor chain, and meteorological sensors that provide fundamental information on lake thermal structure, weather conditions, and lake metabolism. Data are usually collected either at 1 minute or 10 minute intervals. The D-Opto dissolved oxygen sensor is 0.5m from the lake surface, thermistors are at the surface, at 0.25 m and at every .5 m from 0.5 m to 4.5 m, and meteorological sensors measure wind speed, wind direction, relative humidity, and air temperature. The buoy is also equipped with a CO2 monitor and a YSI AutoProfiler that measures several parameters including dissolved oxygen, water temperature, conductivity, pH, ORP, turbulence and chlorophyll-a. After correcting for flux to or from the atmosphere and vertical mixing within the water column, high frequency measurements of dissolved gases such as carbon dioxide and oxygen can be used to estimate gross primary productivity, respiration, and net ecosystem productivity, the basic components of whole lake metabolism. Sampling Frequency: varies for instantaneous sample. Generally 1 minute or 10 minutes. Number of sites: 1
Molecular composition of dissolved organic matter in NTL-LTER lakes detected by Fourier-transform ion cyclotron resonance mass spectrometry
The composition of dissolved organic matter (DOM) varies widely in the environment due to distinct sources of the material and subsequent processing. DOM composition drives its reactivity in terms of many processes including photochemical reactions, microbial metabolism, and carbon cycling within water bodies. This study uses ultra-high resolution mass spectrometry via a Fourier-transform ion cyclotron resonance mass spectrometer (FT-ICR MS) to evaluate DOM composition at the molecular level to determine differences in DOM composition among the NTL-LTER lakes. Whole water samples were collected from the surface of each lake near the shore on August 18th and 19th in 2016 in. Ultraviolet-visible spectra were recorded as light absorbance can also give information about DOM composition. Additionally, concentrations of anions, cations, and pH were measured waters because these can all alter DOM reactivity in the environment. Both water chemistry and DOM composition vary widely among the lakes with the bogs displaying the most terrestrial-like signature in DOM and the oligotrophic lakes show more microbial-like or environmentally processed DOM.
Molecular composition of dissolved organic matter from Lake Mendota from June – November 2017, analyzed by Fourier-transform ion cyclotron resonance mass spectrometry
Dissolved organic matter (DOM) is a complex mixture of organic compounds found in all natural waters. Its composition affects its reactivity towards numerous processes. Its composition is a function of both its source (e.g., allochthonous or autochthonous) as well as the extent of environmental processing it has undergone (e.g., chemical or biological degradation). Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS) allows for the characterization of dissolved organic matter at the molecular level. The water sample was collected near the NTL-LTER research buoy on Lake Mendota. Formula assignments were made to raw mass to charge ratios detected in the mass spectrum using a custom processing script and resulting in a list of chemical formulas making up the DOM sample.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
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