Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
127
datasets available to search
ShareScore release 0.9.0
Dataset results
127 results for “temperature sensitivity”
Comparison of tetracycline and temperature sensitive GAL80 transgenes with UAS-lacZ
<p>ß-galactosidase specific activity was measured during development and aging (Experimental workflow) as described in <a href="https://star-protocols.cell.com/protocols/2150">STAR Protocols 3, 101843, 2022</a>. Two different muscle-specific GAL4 drivers, Mef2-Gal4 (BDSC:27390) and DJ694 (BDSC: 8176), were each crossed with a second chromosome insertion of UAS-lacZ (Bg2) (BDSC: 1776), Bg2 recombined with third chromosome insertions of TetOFF-GAL80 (3.3+1077, <a href="https://peerj.com/articles/4167/">PeerJ 5:e4167</a>), and Bg2 recombined with third chromosome insertions of GAL80ts (BDSC:7017). Flies were raised at 20˚C and 29℃. Two independent sets of parents were used for each cross (biological replicates 1 and 2). After 2-3 days, parents were flipped into a new bottles (technical replicates 1 and 2 reported as replicate 3 and 4 in the dataset). CPRG and Bradford assays were performed on whole animal for the third instar larvae (L3), early pupae (EP) and late pupae (LP) stages, and on dissected thoraces for adult stages. Five extracts (1 individual in each) were measured per experimental replicate. Raw microplate readings are available upon request.</p>
Comparison of tetracycline and temperature sensitive GAL80 transgenes with UAS-grim
<p>In order to assess the ability of GAL80 to repress the expression of a UAS transgene, lethality tests were performed in presence of a UAS-grim reporter (<a href="http://doi.org/10.1002/gene.10128"><em>Genesis</em> 34:34, 2002</a>, <a href="https://peerj.com/articles/4167/"><em>PeerJ</em> 5:e4167, 2017</a>). The Grim reporter gene encodes a strong pro-apoptotic factor, thus the lethality across development (embryonic, larval, pupal) can be scored to assess the repression ability of GAL80. The UAS-grim lethality test facilitates the determination of the developmental period during which GAL4 activity is not inhibited.</p> <p>Two different muscle-specific GAL4 drivers, Mef2-Gal4 (BDSC:27390) and DJ694 (BDSC: 8176), were each crossed with a second chromosome insertion of UAS-grim (<a href="https://doi.org/10.1038/sj.cdd.4400423">Cell Death Differ 5:930,1998</a>), UAS-grim recombined with third chromosome insertions of GAL80TET (3.3+1077, <a href="https://peerj.com/articles/4167/">PeerJ 5:e4167</a>), and UAS-grim recombined with third chromosome insertions of GAL80ts (BDSC:7017). Negative control (no lethality) was generated by crossing the UAS-grim strain with a strain without any GAL4 transgene (w<sup>1118</sup>,<a href="https://www.cell.com/cell/pdf/0092-8674(84)90240-X.pdf?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2F009286748490240X%3Fshowall%3Dtrue">Cell 36:469, 1984</a> ). Crosses were maintained, and eggs were collected at 20˚C (Experimental workflow). Parents were obtained from multiple independent cultures to set-up independent crosses (biological replicates, Set indicates parents from the same culture). Crosses were kept 24 to 48h in culture tubes to allow mating before transferring them to egg collectors. Multiple egg collections were done for each parental set (technical replicates, Date indicates dates of collection). Parents were allowed to lay eggs for 12h to 16h. Up to 25 eggs were aligned on a slice of food and up to 4 slices were used for a given collection from a set. Slices of food are then transferred to culture vials and incubated at the indicated temperature. The scoring of the number of first-instar larvae (L1) was done by scoring the number of empty eggs 25-30h (29˚C) or 43-48h (20˚C) after egg alignment. The number of pupae and adults was scored 5-6 days (29˚C) or 8-10 days (20˚C) after the scoring of the previous stage.</p>
dataset of: "Glacier projections sensitivity to temperature-index model choices and calibration strategies"
<p>data for <strong><em>Schuster, L., Rounce, D. R., and Maussion, F.: Glacier projections sensitivity to temperature-index model choices and calibration strategies, Annals of Glaciology, 2023, <a href="https://doi.org/10.1017/aog.2023.57" target="_blank" rel="noopener"><span>https://doi.org/10.1017/aog.2023.57 </span></a></em></strong><br><br><strong>When using this dataset, please refer to the original publication in addition to this Zenodo repository.</strong></p> <p>You can find the code to create the data and plot the figures in the <a href="https://github.com/lilianschuster/oggm_mb_sandbox_option_intercomparison">oggm_mb_sandbox_option_intercomparison GitHUB repository</a>.<br> </p>
Dataset for: Temperature sensitivity of carbon concentrating mechanisms in the diatom Phaeodactylum tricornutum
<p><span>Marine diatoms are key primary producers across diverse habitats in the global ocean. Diatoms rely on a biophysical carbon concentrating mechanism (CCM) to supply high concentrations of CO<sub>2</sub> around their carboxylating enzyme, RuBisCO. The necessity and energetic cost of the CCM are likely to be highly sensitive to temperature, as temperature impacts </span><span>CO<sub>2</sub></span><span> concentration, diffusivity, and the kinetics of CCM components. Here, we used membrane inlet mass spectrometry (MIMS) and modeling to capture temperature regulation of the CCM in the diatom <em>Phaeodactylum</em> <em>tricornutum</em> (<em>Pt</em>). We found that enhanced carbon fixation rates by <em>Pt</em> at elevated temperatures were accompanied by increased CCM activity capable of maintaining RuBisCO close to CO2 saturation but that the mechanism varied. At 10 and 18 °C, diffusion of </span><span>CO<sub>2</sub></span><span> into the cell, driven by <em>Pt</em>'s 'chloroplast pump' was the major inorganic carbon source. However, at 18 °C, upregulation of the chloroplast pump enhanced (while retaining the proportion of) both diffusive </span><span>CO<sub>2</sub></span><span> and active </span><span>HCO<sub>3</sub></span><sup><span>-</span></sup><span> uptake into the cytosol, and significantly increased chloroplast </span><span>HCO<sub>3</sub></span><sup><span>-</span></sup><span> concentrations. In contrast, at 25 °C, compared to 18 °C, the chloroplast pump had only a slight increase in activity. While diffusive uptake of </span><span>CO<sub>2</sub></span><span> into the cell remained constant, active </span><span>HCO<sub>3</sub></span><sup><span>-</span></sup><span> uptake across the cell membrane increased resulting in <em>Pt</em> depending equally on both </span><span>CO<sub>2</sub></span><span> and HCO<sub>3</sub></span><sup><span>-</span></sup><span> as inorganic carbon sources. Despite changes in the CCM, the overall rate of active carbon transport remained double that of carbon fixation across all temperatures tested. The implication of the energetic cost of the <em>Pt</em> CCM in response to increasing temperatures was discussed.</span></p>
Timing of increased temperature sensitivity coincides with nervous system development in winter moth embryos
Open the record for dataset details and reuse information.
Data from: The thermal sensitivity of growth and survival in a wild reptile with temperature-dependent sex determination
Open the record for dataset details and reuse information.
Data from: Differential pulse sensitivity of nitric and nitrous oxide emissions to temperature, carbon, and nitrogen following wetting of desert soils
Open the record for dataset details and reuse information.
Dataset and codes for: Partitioning the apparent temperature sensitivity between autotrophic and heterotrophic protists
Open the record for dataset details and reuse information.
The effect of a temperature‐sensitive prophage on the evolution of virulence in an opportunistic bacterial pathogen
Open the record for dataset details and reuse information.
Universal temperature sensitivity of denitrification nitrogen losses in forest soils
Open the record for dataset details and reuse information.
Dataset for: Temperature sensitivity of carbon concentrating mechanisms in the diatom Phaeodactylum tricornutum
Open the record for dataset details and reuse information.
Developmental temperature alters the thermal sensitivity of courtship activity and signal-preference relationships, but not mating rates
Open the record for dataset details and reuse information.
Making Nd3+ a Sensitive Luminescent Thermometer for Physiological Temperatures — An Account of Pitfalls in Boltzmann Thermometry
<p> Dataset accompanying figures published in the publication DOI: 10.3390/nano10030543</p>
Data from: Differing thermal sensitivities in a host-parasitoid interaction: high, fluctuating developmental temperatures produce dead wasps and giant caterpillars
<p>1. Insect parasitoids, and the arthropod hosts they consume during development, are important ecological players in almost all environments across the globe. As ectothermic organisms, both parasitoid and host are strongly impacted by environmental temperature. If thermal tolerances differ between host insect and parasitoid, then the outcome of their interaction will be determined by the ambient temperature. As mean temperatures continue to rise and extreme temperatures become more frequent, we must determine the effect of high temperature stress on host-parasitoid systems to predict how they will fare in the face of climate change.</p> <p class="MsoNoSpacingCxSpMiddle">2. The majority of studies conducted on host-parasitoid systems focus on either performance under constant temperature, or a fixed metric of thermal tolerance (CT<sub>max</sub>) for individual organisms. However, performance at constant temperatures is not predictive of performance under ecologically relevant, fluctuating temperatures, and measurements of thermal thresholds provide little information regarding the effects of temperature throughout development. We address this by testing the effects of increasing mean temperature in both constant and fluctuating (±10°C) environments throughout development on the performance of the parasitoid wasp <i>Cotesia congregata</i> and its lepidopteran larval host, <i>Manduca sexta.</i></p> <p class="MsoNoSpacingCxSpLast">3. The growth of <i>M. sexta</i> was influenced by mean temperature, diurnal fluctuations, and parasitization status. Caterpillar growth rate increased with increasing mean temperature, but decreased in response to diurnal fluctuations and parasitization by <i>C. congregata </i>wasps.</p> <p>4. Wasp survival decreased with increasing mean temperature, and diurnal fluctuations decreased wasp survival, especially at higher mean temperatures. Diurnal fluctuations at our highest mean temperature treatment (30°C±10°C) resulted in complete wasp mortality, and parasitized hosts displayed abnormal physiology, wherein they failed to exhibit wasp emergence, did not enter the prepupal stage, continued to feed, and grew up to two-fold larger than a normal, unparasitized caterpillar.</p> <p>5. Our results indicate hosts and parasitoids in this system have different thermal tolerances during development; the parasitoid wasp suffered complete mortality at a temperature regime that is mildly stressful for the unparasitized caterpillar host species. Our findings suggest <i>C. congregata </i>will suffer more severely under increasing temperatures than <i>M. sexta</i>, with cascading trophic and ecological effects.</p>
Data from: Latitudinal clines in the timing and temperature-sensitivity of photoperiodic reproductive diapause in Drosophila montana
<p>Reproductive diapause is a primary mechanism used by arthropods to synchronize their life cycle with seasonal changes in temperate regions. Our study species, Drosophila montana, represents the northern insect species where flies enter reproductive diapause under short day conditions and where the precise timing of diapause is crucial for both survival and offspring production. We have studied clinal variation in the critical day length for female diapause induction (CDL) and their overall susceptibility to enter diapause (diapause incidence), as well as the temperature sensitivity of these traits. The study was performed using multiple strains from four latitudinal clines of the species – short clines in Finland and Alaska and long clines in the Rocky Mountains and the western coast of North America - and from one population in Kamchatka, Russia. CDL showed strong latitudinal clines on both continents, decreasing by one hour per five degrees decline in latitude, on average. CDL also decreased in all populations along with an increase in fly rearing temperature postponing the diapause to later calendar time, the effects of temperature being stronger in southern than in northern population. Female diapause incidence was close to 100 % under short day / low temperature conditions in all populations, but decreased below 50 % even under short days in 19°C in the southern North American western coast populations and in 22°C in most populations. Comparing a diversity of climatic data for the studied populations showed that while CDL is under a tight photoperiodic regulation linked with latitude, its length depends also on climatic factors determining the growing season length. Overall, the study deepens our understanding of how spatial and environmental parameters affect the seasonal timing of an important biological event, reproductive diapause, and helps to estimate the evolutionary potential of insect populations to survive in changing climatic conditions.</p>
Data from: Sea-surface temperature pattern effects have slowed global warming and biased warming-based constraints on climate sensitivity
<p>The observed rate of global warming since the 1970s has been proposed as a strong constraint on equilibrium climate sensitivity (ECS) and transient climate response (TCR) – key metrics of the global climate response to greenhouse-gas forcing. Using CMIP5/6 models, we show that the inter-model relationship between warming and these climate sensitivity metrics (the basis for the constraint) arises from a similarity in transient and equilibrium warming patterns within the models, producing an effective climate sensitivity (EffCS) governing recent warming that is comparable to the value of ECS governing long-term warming under CO<sub>2</sub> forcing. However, CMIP5/6 historical simulations do not reproduce observed warming patterns. When driven by observed patterns, even high ECS models produce low EffCS values consistent with the observed global warming rate. The inability of CMIP5/6 models to reproduce observed warming patterns thus results in a bias in the modeled relationship between recent global warming and climate sensitivity. Correcting for this bias means that observed warming is consistent with wide ranges of ECS and TCR extending to higher values than previously recognized. These findings are corroborated by energy balance model simulations and coupled model (CESM1-CAM5) simulations that better replicate observed patterns via tropospheric wind nudging or Antarctic meltwater fluxes. Because CMIP5/6 models fail to simulate observed warming patterns, proposed warming-based constraints on ECS, TCR, and projected global warming are biased low. The results reinforce recent findings that the unique pattern of observed warming has slowed global-mean warming over recent decades, and that how the pattern will evolve in the future represents a major source of uncertainty in climate projections.</p>
Data used in JAMES paper "Sensitivity of the Horizontal Scale of Convective Self‐Aggregation to Sea Surface Temperature in Radiative Convective Equilibrium Experiments Using a Global Nonhydrostatic Model"
<p>Data used in JAMES paper "Sensitivity of the Horizontal Scale of Convective Self‐Aggregation to Sea Surface Temperature in Radiative Convective Equilibrium Experiments Using a Global Nonhydrostatic Model" by Shuhei Matsugishi and Masaki Satoh doi: 10.1029/2021MS002636</p>
Stay in shape: assessing the adaptive potential of shell morphology and its sensitivity to temperature in the invasive New Zealand Mud Snail Potamopyrgus antipodarum through phenotypic plasticity and natural selection in Europe
<p>Climate change may force organisms to adapt genetically or plastically to new environmental conditions. Invasive species show a remarkable potential for rapid adaptation. The ovoviviparous New Zealand mud snail (NZMS), <em>Potamopyrgus antipodarum</em>, has successfully established across Europe with two clonally reproducing mitochondrial lineages since its arrival in the first half of the 19th century. Its remarkable variation in shell morphology was shown to be fitness relevant. We investigated the effects of temperature on shell morphology across eleven populations from Germany and the Iberian Peninsula in a common garden across three temperatures. We analysed size and shape using geometric morphometrics. For both, we compared reaction norms and estimated heritabilities. For size, the interaction of temperature and haplotype explained about 50% of the total variance. We also observed more genotype by environment interactions indicating a higher degree of population differentiation than in shape. Across the three temperatures, size followed the expectations of the temperature-size rule, with individuals growing larger in cold environments. Changes in shape may have compensated changes in size affecting space for brooding embryos. Heritability estimates were relatively high. As indicated by the very low coefficients of variation for clonal repeatability (<em>CV<sub>A</sub></em>), they can probably not be compared in absolute terms. However, they showed some sensitivity to temperature, in haplotype t more so than in z, which was only found in Portugal. The low <em>CV<sub>A</sub></em>-values indicate that genetic variation among European populations is still restricted with low potential to react to selection. A considerable fraction of the genetic variation was due to differences between the clonal lineages. The NZMS has apparently not been long enough in Europe to accumulate significant genetic variation relevant for morphological adaptation. As temperature is obviously not the sole factor influencing shell morphology, their interaction will probably not be a factor limiting population persistence under a warming climate in Europe.</p>
Data from: Declining temperature and increasing moisture sensitivity of shrub growth in the Low-Arctic erect dwarf-shrub tundra of western Greenland
<p>Evergreen dwarf shrubs respond swiftly to warming in the cool and dry High Arctic, but their response in the warmer Low Arctic, where they are expected to be outcompeted by taller species under future warming, remains to be clarified.</p> <p>Here, 12,528 annual growth increments, covering 122 years (1893-2014), were measured of 764 branches from 25 individuals of the evergreen dwarf shrub <em>Cassiope tetragona </em>from a Low-Arctic erect dwarf-shrub tundra site in western Greenland. In addition, branch initiation and mortality frequency time series were developed. The influence of seasonal climate and correspondence with fluctuations in regional normalized difference vegetation index (NDVI), a satellite proxy for vegetation productivity, were studied.</p> <p>Summer temperatures were the main driver of growth, while winter temperatures were the main non-summer-climate driver. During past and recent warm episodes, shrub growth diverged from summer temperatures. In recent decades, early summer precipitation has become the main growth-limiting factor for some individuals, likely through micro-topography-determined soil moisture availability, and more than half of the shrubs studied became irresponsive to summer temperatures. There was correspondence between climatic drivers, <em>C. tetragona</em> growth and branch initiation frequency, and satellite-observed vegetation productivity, suggesting the area's shrub-dominated tundra vegetation is limited by similar climatic factors. Winter warming events were likely the predominant cause of branch mortality, while branching increased after years of poor growth and cooler-than-average summers.</p> <p><strong>Synthesis</strong>: These findings show that the erect dwarf-shrub tundra in the Low Arctic has already and will likely become decreasingly temperature- and increasingly moisture-limited and that winter warming supports shrub growth, but increased extreme winter warming event frequency may increase branch mortality and vegetation damage. Such counter-acting mechanisms could offer an explanation for the vegetation stability observed over large parts of the Arctic.</p>
Cyanobacterial- and moss-forming biocrusts consistently mitigate the temperature sensitivity of microbial respiration along a continental precipitation gradient
<p>1. Biocrusts are prevalent and participate in many soil organic carbon (C) processes in drylands. The predicted increase in aridity will expand the biocrust cover and significantly impact soil organic C dynamics. However, how biocrusts change soil organic C decomposition and what factors drive the effect in response to climate warming remains largely unknown at a continental scale.</p> <p>2. We measured microbial respiration and its temperature sensitivity (Q10) in bare soil lacking biocrusts and two universal biocrusted soils (cyanobacterial- and moss-crusted soil) from 43 sites across a precipitation gradient from 39 mm to 443 mm to evaluate the relative effects of biocrusts on Q10 and the driving forces in northern China's dryland.</p> <p>3. Microbial respiration increased and Q10 decreased with increasing precipitation in bare soil, cyanobacterial- and moss-crusted soil. Biocrusts positively affected microbial respiration, with a more substantial magnitude by moss crusts than cyanobacterial crusts. Biocrusts negatively impacted Q10, and the magnitudes were similar between moss and cyanobacterial crusts. Most importantly, the relative effects of biocrusts on microbial respiration and Q10 increased with decreasing precipitation.</p> <p>4. The positive effects of biocrusts on soil organic C content and microbial biomass carbon were positively correlated with the level of increased microbial respiration. Contrastingly, the magnitude of reduced Q10 was attributed to the biocrusts' positive effects on soil organic C quality and adverse effects on the ratio of fungal to bacterial PLFAs (F: B).</p> <p>5. Our study provides strong evidence that biocrusts decrease the temperature sensitivity of microbial respiration in northern China's dryland. This result suggests that the predicted expanding biocrust cover is crucial for maintaining the soil organic C stability by buffering the positive impacts of climate warming on soil organic C decomposition in drylands.</p>
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.