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29,897 results for “activism”
Fig. 1 in Antioxidant activity of bee products added to water in tebuconazole-exposed fish
Fig. 1. Levels of TBARS (nmol MDA mg-1 protein) and GSH (µmol GSH g-1 of wet tissue) in Rhamdia quelen after exposure to 16.6% of LC 50 of tebuconazole, to bee product, and to tebuconazole + bee product for 96 h. Different small letters indicates statistical differences between the means (ANOVA followed by Tukey´s multiple range test). Mean ± SEM; n = 10. * P <0.05.
Increase of active-power-based flexibility (data for KPI evaluation)
<p>There are stored data collected from new EV charging stations – this will be used as an aggregated source of active power-based flexibility procured for system operator (DSO) and managed through non frequency platform. Data originated from part of the CZ DEMO run directly by ČEZ distribuce called "e – fleet". At Zenodo there are data from all sites (EV charging poles while the first excel sheet contains calculation of the KPI “Increase of active-power-based flexibility”. Detailed explanation on KPI evaluation, data format and main findings from the tests are included in the <a title="link" href="https://www.onenet-project.eu/wp-content/uploads/2024/03/OneNet_D10.5_V1.0.pdf">Deliverable 10.5 (section 2.1.2). </a></p>
Mosquitoes escape looming threats by actively steering into the bow-wave induced by the attacker
<p>To detect and escape a threat, night flying insects must rely on other senses than vision alone. Here we study how anthropophilic malaria mosquitoes can escape a swatting hand in the dark using high-speed videography and numerical simulations. We show that these night flying mosquitoes escape looming objects by using the object-induced airflow in two ways. They first actively steer into the <a>bow-wave</a><span><span> </span></span> produced by the attacker, and then passively travel with this bow-wave away from the attacker; these two aspects explain two-thirds and one-third of their escape accelerations, respectively. Thus, flying mosquitoes being attacked in the dark rely both on airflow-sensing to trigger their escape, and on attacker-induced airflow to maximize their escape performance. Similar escape strategies are probably common among small lightweight insects.</p> <div> <div> <div></div> </div> </div>
HDX-MS dataset for: "Glycan-induced structural activation softens the human papillomavirus capsid for entry through reduction of intercapsomere flexibility"
<p>Hydrogen/deuterium exchange mass spectrometry dataset used in: <strong>Glycan-induced structural activation softens the human papillomavirus capsid for entry through reduction of intercapsomere flexibility.</strong> Yuzhen Feng*, Dominik van Bodegraven*, Alan Kádek*, Ignacio L.B. Munguira, Laura Soria-Martinez, Sarah Nentwich, Sreedeepa Saha, Florian Chardon, Daniel Kavan, Charlotte Uetrecht#, Mario Schelhaas#, Wouter H. Roos#. <em>Nature Communications</em> 10076 (2024). doi: 10.1038/s41467-024-54373-0</p> <p>* - authors contributing equally</p> <p># - corresponding authors</p> <p><strong>Description:</strong></p> <p>Hydrogen/deuterium exchange mass spectrometry (HXMS) analysis of the effect of heparin on the conformational dynamics of human papillomavirus 16 pseudovirus (PsV).</p> <p><strong>Sample processing:</strong></p> <p>HPV16 PsV were prepared according to (Buck & Thompson: Current Protocols in Cell Biology 2007). In short, p16Shell and pClneo-EGFP were transfected into HEK293TT cells. After 48 h, cells were harvested and lysed followed by maturation of the virus particles for 24 h. For purification, the particles were purified using a CsCl step gradient (27 % w/V and 38.8 % w/V CsCl in 10 mM Tris-HCl pH 7.4, 207570 x g, 3 h 50 min, 4 °C) followed by dialysis in Float-A-Lyzer devices (1 mL, Spectra/Por) against a total of 3 L HPV virion buffer (1x PBS, 635 mM NaCl, 0.9 mM CaCl2, 0.5 mM MgCl2, 2.1 mM KCl, pH 7.4).</p> <p>PsV were pre-incubated for 1 h either with or without heparin (H4784, Sigma-Aldrich) at room temperature. To initiate deuterium labelling the samples were 6-fold diluted with the virion buffer they were obtained in, only made of 99.9% D2O (150 mM NaCl, 4.8 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 0.9 mM CaCl2, 0.5 mM MgCl2, pD 7.2). This resulted in a final concentration of 0.5 µM L1 monomer in the form of PsV with or without 1 mg/ml heparin during deuterium labelling. The exchange reaction was left to proceed at room temperature until aliquots of 45 µl were removed at predetermined time points (1 min, 5 min, 15 min, 1 h and 4 h). In the aliquots, the exchange was immediately stopped by twofold dilution with ice-cold quench buffer (0.25 M glycine, 100 mM TCEP, 8 M urea, indicated pH 2.7), resulting in final pH 2.5. For samples with heparin, the quench buffer additionally contained 1 mg/ml protamine sulphate (P4020, Sigma-Aldrich). After 30 s incubation on ice, the samples were centrifuged at 10.000 x g for 1 min at 0 °C. Each supernatant was transferred to a fresh tube and flash frozen in liquid nitrogen. Low binding microtubes and low binding pipette tips (both Axygen) were used throughout for all handling of viral particles.</p> <p>The frozen samples were quickly thawed and injected into a refrigerated (1°C) HPLC system (Infinity 1260, Agilent Technologies), through a porcine pepsin column (≥ 3200 units/mg, Sigma-Aldrich) in-house immobilized onto POROS-20AL perfusion resin (Thermo Scientific) as described previously (Wang et al.: Molecular & Cellular Proteomics 2002), which was kept at 4°C. Pepsin digestion was performed at isocratic 200 µl/min flow rate (0.4 % formic acid in water). After the digestion, peptides were online desalted for 3 min on a peptide microtrap (OPTI-TRAP, Optimize Technologies) and then eluted on a reversed-phase analytical column (ZORBAX 300SB-C18, 0.5 x 35 mm, 3.5 µm, 300Å, Agilent Technologies). There LC separation proceeded at 25 µl/min flow rate through an 8 min gradient of 8–30% solvent B, followed by a 3 min gradient of 30-90 % solvent B (solvent A: 0.4 % formic acid in water, solvent B: 0.4 % formic acid in acetonitrile). The outlet of the HPLC system was connected to an electrospray ionization (ESI) source of an Orbitrap Fusion Tribrid Mass Spectrometer (Thermo Scientific). The instrument was operated in positive ESI MS-only mode for deuterated samples, scan range 300-2000 m/z, using 4 microscans at resolving power setting 120,000. In a separate measurement on non-deuterated sample, the instrument was used in positive data-dependent ESI MS/MS mode with 30% HCD dissociation, 1 microscan and 240,000 resolving power setting for the identification of all peptides produced by non-specific pepsin cleavage.</p> <p>In total 22 pmol and 50 pmol L1 protein were injected per MS and MS/MS analysis, respectively. To minimize sample carry-over on the protease column, two washing solutions were always injected between sample injections modified from Majumdar et al. 69 (wash solution 1: 5% acetonitrile, 5% isopropanol, 20% acetic acid; wash solution 2: 4 M Urea, 1 M glycine, pH 2.5). All HDX samples were analysed in technical triplicates, except for the 15 min time point for PsV without heparin, which was measured in duplicate.</p> <p><br><strong>Data processing:</strong></p> <p>Peptides were identified from the MS/MS data by the Andromeda search algorithm implemented in MaxQuant (version 1.6.5.0) using a custom protein database containing the sequences of HPV16 L1 and L2 proteins. Deuterium uptake for the identified peptides was calculated with DeutEx (in-house developed), manually inspected and the statistical significance of the observed differences in deuteration was evaluated by applying an unpaired two-tailed Student’s T-test with single pooled variance evaluated with alpha ≤ 0.05 using the Holm-Šidák correction for multiple comparisons in Prism 8.0.1 (GraphPad Software). The processed data were visualized using MSTools (https://peterslab.org/MSTools/, Kavan & Man: International Journal of Mass Spectrometry 2011) and open-source PyMol 2.6.0a0 (Schrödinger, Inc).</p> <p>For ZENODO the datafiles were deposited as native Thermo .raw files (including instrumental parameters metadata) while all peaks in the spectra were additionally exported into plain m/z vs intensity .txt files per each scan in the LC-MS analysis as also used for the DeutEx HDX-MS processing.</p>
Image quantification data for: Activity-dependent mitochondrial ROS signaling regulates recruitment of glutamate receptors to synapses
<p>Our understanding of mitochondrial signaling in the nervous system has been limited by the technical challenge of analyzing mitochondrial function <em>in vivo</em>. In the transparent genetic model <em>Caenorhabditis elegans, </em>we were able to manipulate and measure mitochondrial ROS (reactive oxygen species) signaling of individual mitochondria as well as neuronal activity of single neurons <em>in vivo</em>. Using this approach, we provide evidence supporting a novel role for mitochondrial ROS signaling in dendrites of excitatory glutamatergic <em>C. elegans</em> interneurons. Specifically, we show that following neuronal activity, dendritic mitochondria take up calcium (Ca<sup>2+</sup>) via the mitochondrial Ca<sup>2+</sup> uniporter MCU-1 which results in an upregulation of mitochondrial ROS production. We also observed that mitochondria are positioned in close proximity to synaptic clusters of GLR-1, the <em>C. elegans</em> ortholog of the AMPA subtype of glutamate receptors that mediate neuronal excitation. We show that synaptic recruitment of GLR-1 is upregulated when MCU-1 function is pharmacologically or genetically impaired but is downregulated by mitoROS signaling. Thus, signaling from postsynaptic mitochondria may regulate excitatory synapse function to maintain neuronal homeostasis by preventing excitotoxicity and energy depletion.</p>
Automatic monitoring of neural activity with single-cell resolution in behaving Hydra
<p>The ability to record every spike from every neuron in a behaving animal is one of the holy grails of neuroscience. Here, we report coming one step closer towards this goal with the development of an end-to-end pipeline that automatically tracks and extracts calcium signals from individual neurons in the cnidarian <em>Hydra vulgaris</em>. We imaged dually labeled (nuclear tdTomato and cytoplasmic GCaMP7s) transgenic <em>Hydra </em>and developed an open-source Python platform (TraSE-IN) for the Tracking and Spike Estimation of Individual Neurons in the animal during behavior. The TraSE-IN platform comprises a series of modules that segments and tracks each nucleus over time and extracts the corresponding calcium activity in the GCaMP channel. Another series of signal processing modules allows robust prediction of individual spikes from each neuron's calcium signal. This complete pipeline will facilitate the automatic generation and analysis of large-scale datasets of single-cell resolution neural activity in <em>Hydra</em>, and potentially other model organisms, paving the way towards deciphering the neural code of an entire animal.</p>
Short-range correlation of stress chains near solid-to-liquid transition in active monolayers
<p>Using a three-dimensional model of cell monolayers, we study the spatial organization of active stress chains as the monolayer transitions from a solid to a liquid state. The critical exponents that characterize this transition map the isotropic stress percolation onto the two-dimensional random percolation universality class suggesting short-range stress correlations near this transition. This mapping is achieved via two distinct, independent pathways: (i) cell-cell adhesion and (ii) active traction forces. We unify our findings by linking the nature of this transition to high-stress fluctuations, distinctly linked to each pathway. The results elevate the importance of the transmission of mechanical information in dense active matter and provide a new context for understanding the non-equilibrium statistical physics of phase transition in active systems.</p>
Dataset The Lactate Receptor HCAR1: a Key Modulator of Epileptic Seizure Activity
<p>This dataset is related to the study: </p> <p>The Lactate Receptor HCAR1: a Key Modulator of Epileptic Seizure Activity</p> <p>by Maxime Alessandri (1), Alejandro Osorio-Forero (1), Anita Luthi (1) & Jean-Yves Chatton* (1)</p> <p><span>(1) Department of Fundamental Neurosciences, University of Lausanne, 1005 Lausanne, Vaud, Switzerland.</span></p>
Top view of DR1/DR2 double riffle, each section contains a spawning ground made up of eight gravel-filled trays, a rest area. The "double riffle" was designed to accommodate two groups from 25 to 50 specimens of broodstock in strictly identical conditions. The spawning grounds are equipped with waterproof, motion-sensing cameras with infrared night vision, connected to a 1000 Gb recorder. The diurnal and nocturnal activities of the two groups can therefore be simultaneously recorded over a long period. in Reproduction of Zingel asper (Linnaeus, 1758) in controlled conditions: an assessment of the experiences realized since 2005 at the Besançon Natural History Museum
Top view of DR1/DR2 double riffle, each section contains a spawning ground made up of eight gravel-filled trays, a rest area. The "double riffle" was designed to accommodate two groups from 25 to 50 specimens of broodstock in strictly identical conditions. The spawning grounds are equipped with waterproof, motion-sensing cameras with infrared night vision, connected to a 1000 Gb recorder. The diurnal and nocturnal activities of the two groups can therefore be simultaneously recorded over a long period.
Data from: "Rare earth elements sediment analysis tracing anthropogenic activities in the stratigraphic sequence of Alagankulam (India)"
Open the record for dataset details and reuse information.
Dataset for the publication: Potential of alcohol fuels in active and passive pre-chamber applications in a passenger car spark-ignition engine
<div> <p>The dataset covers the research data of the publication "Potential of alcohol fuels in active and passive pre-chamber applications in a passenger car spark-ignition engine" in International Journal of Engine Research (DOI: 10.1177/14680874211053168).</p> </div>
F I G U R E 1 3 in Analysis of pigment cell composition, pigment content, tyrosinase content and activity of three kinds of loaches Misgurnus anguillicaudatus from Poyang Lake
F I G U R E 1 3 Tyrosinase content of three kinds of loaches (*means P <0.05, **means P <0.01). () BBL; () SBL; () NBL; () dorsal skin; () abdominal skin
F I G U R E 3 in Analysis of pigment cell composition, pigment content, tyrosinase content and activity of three kinds of loaches Misgurnus anguillicaudatus from Poyang Lake
F I G U R E 3 The distribution of skin pigment cells in abdomen of three kinds of loaches. (a) The abdominal epidermis of big blackspot loaches (BBL), (b) the abdominal epidermis of small blackspot loaches (SBL) and (c) the abdominal epidermis of non-blackspot loaches (NBL). The blue arrow refers to xanthophores. The magnification (a–c) is 80
F I G U R E 9 in Analysis of pigment cell composition, pigment content, tyrosinase content and activity of three kinds of loaches Misgurnus anguillicaudatus from Poyang Lake
F I G U R E 9 Ultrathin sections of skin of three kinds of loaches. (a) The dorsal skin of big blackspot loaches (BBL), (b) the dorsal skin of small blackspot loaches (SBL), (c) the dorsal skin of non-blackspot loaches (NBL), (d) the abdominal skin of BBL, (e) the abdominal skin of SBL and (f) the abdominal skin of NBL; N refers to nucleus in the six figures (a–f); S refers to stratum corneum in the six figures (a–f); (g) the dorsal skin of BBL, (h) the dorsal skin of SBL, (i) the dorsal skin of NBL, (j) the abdominal skin of BBL, (k) the abdominal skin of SBL, (l) the abdominal skin of NBL. The magnification of (a–f) is 1500 and the magnification of (g–l) is 6000. The green arrow indicates stage 1; the yellow arrow indicates stage 2; the red arrow indicates stage 3; the black arrow indicates stage 4
F I G U R E 1 1 in Analysis of pigment cell composition, pigment content, tyrosinase content and activity of three kinds of loaches Misgurnus anguillicaudatus from Poyang Lake
F I G U R E 1 1 Lutein content of three kinds of loaches (*means P <0.05, **means P <0.01). () BBL; () SBL; () NBL; () dorsal skin; () abdominal skin
F I G U R E 2 in Analysis of pigment cell composition, pigment content, tyrosinase content and activity of three kinds of loaches Misgurnus anguillicaudatus from Poyang Lake
F I G U R E 2 Distribution of pigment cells on the dorsal epidermis of three kinds of loaches. (a) The dorsal epidermis of big blackspot loaches (BBL), (b) the dorsal epidermis of small blackspot loaches (SBL), (c) the dorsal epidermis of non-blackspot loaches (NBL), (d) the magnification of the dorsal epidermis melanocytes of BBL, (e) the magnification of the dorsal epidermis melanocytes of SBL and (f) the magnification of the dorsal epidermis melanocytes of NBL. The black arrow refers to the first type of melanocytes, and the red arrow refers to the second type of melanocytes. The blue arrow refers to xanthophores. The magnification of (a–c) is 80. The magnification of d is 200. The magnification of (e–f) is 400
F I G U R E 8 in Analysis of pigment cell composition, pigment content, tyrosinase content and activity of three kinds of loaches Misgurnus anguillicaudatus from Poyang Lake
F I G U R E 8 Iridophores in skin of three kinds of loaches. (a) The dorsal skin of big blackspot loaches (BBL), (b) the dorsal skin of small blackspot loaches (SBL), (c) the dorsal skin of non-blackspot loaches (NBL), (d) the abdominal skin of BBL, (e) the abdominal skin of SBL and (f) the abdominal skin of NBL. The black arrow indicates iridophores
F I G U R E 7 in Analysis of pigment cell composition, pigment content, tyrosinase content and activity of three kinds of loaches Misgurnus anguillicaudatus from Poyang Lake
F I G U R E 7 Melanocyte in skin of three kinds of loaches. (a) The dorsal skin of big blackspot loaches (BBL), (b) the dorsal skin of small blackspot loaches (SBL), (c) the dorsal skin of non-blackspot loaches (NBL), (d) the abdominal skin of BBL, (e) the abdominal skin of SBL and (f) the abdominal skin of NBL. The black arrow indicates melanocytes
F I G U R E 4 in Analysis of pigment cell composition, pigment content, tyrosinase content and activity of three kinds of loaches Misgurnus anguillicaudatus from Poyang Lake
F I G U R E 4 Skin microstructure of three kinds of loaches. (a) The dorsal skin of big blackspot loaches (BBL), (b) the dorsal skin of small blackspot loaches (SBL), (c) the dorsal skin of non-blackspot loaches (NBL), (d) the abdominal skin of BBL, (e) the abdominal skin of SBL and (f) the abdominal skin of NBL
F I G U R E 1 4 in Analysis of pigment cell composition, pigment content, tyrosinase content and activity of three kinds of loaches Misgurnus anguillicaudatus from Poyang Lake
F I G U R E 1 4 Tyrosinase activity in three kinds of loaches (*means P <0.05, **means P <0.01). () BBL; () SBL; () NBL; () serum; () dorsal skin; () abdominal skin
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
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The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
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