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Fig. 3 in Salivary gland proteome analysis of developing adult female HaemaphYSaliS longiCorniS ticks: molecular motor and TCA cycle-related proteins play an important role throughout development

Fig. 3 GO functional annotations for all the differentially expressed proteins. a–c GO annotations of differentially expressed proteins in the salivary glands of partially fed ticks compared with unfed ticks (115:114). d–f GO annotations of differentially expressed proteins in the salivary glands of mated semi-engorged ticks compared with partially fed ticks (116:115). g–i GO annotations of differentially expressed proteins in the salivary glands of engorged ticks compared with mated semi-engorged ticks (117:116). Abbreviations: BP, biological process; CC, cellular component; MF, molecular function; CO, cellular component organization or biogenesis

Fig. 5 in Salivary gland proteome analysis of developing adult female HaemaphYSaliS longiCorniS ticks: molecular motor and TCA cycle-related proteins play an important role throughout development

Fig. 5 RT-qPCR analyzed the mRNA expression levels of dynein, kinesin, isocitrate dehydrogenase, and citrate synthase during the four feeding stages of salivary gland development

Fig. 2 in Salivary gland proteome analysis of developing adult female HaemaphYSaliS longiCorniS ticks: molecular motor and TCA cycle-related proteins play an important role throughout development

Fig. 2 Statistics and cluster analysis for the identified proteins and their expression levels in the salivary glands of female H. longicornis. a Venn diagram showing the number of proteins (with CV <20%) identified in the three experiments. b Venn diagram showing the number of proteins with quantitative information. c Cluster analysis according to trends in protein expression in the salivary glands of female ticks

Fig. 1 in Salivary gland proteome analysis of developing adult female HaemaphYSaliS longiCorniS ticks: molecular motor and TCA cycle-related proteins play an important role throughout development

Fig. 1 Workflow for quantitative proteomics analysis of changes in protein expression in the salivary glands of female H. longicornis during the blood-feeding process

opencc-by-4.0Apr 2024View details →

Development of a desorption electrospray ionization –multiple-reaction-monitoring mass spectrometry (DESI-MRM) workflow for spatially mapping oxylipins in pulmonary tissue

Data from desorption electrospray ionization mass spectrometry – multiple-reaction-monitoring mass spectrometry (DESI-MRM) analysis of oxylipins in guinea pig lung tissue following in vivo exposure to house dust mite extract. Data are provided as Waters *.raw data folders, each incuding an 'Analyte .txt' file, which is generated from processing within MassLynx (Waters). The 'ion_library.txt' file includes details about the MRM transitions and is required for processing the data with quantMSImageR (<a href="https://github.com/targeted-lipidomics/quantMSImageR">https://github.com/targeted-lipidomics/quantMSImageR</a>).

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Data from: Deletion of an sRNA primes development in a multicellular bacterium

Small non-coding RNAs (sRNAs) are essential in regulating gene expression during many biological processes. The myxobacteria gene pxr encodes an sRNA known to block fruiting-body development, an aggregative multicellular process triggered by starvation. Deletion of pxr allows Myxococcus xanthus cells to develop in the presence of nutrients. However, potential Pxr binding targets and most genes regulated by Pxr remain unknown. Here, we found that the absence of pxr expression dramatically alters the temporal dynamics of development, thus suggesting an important new role of this sRNA in myxobacterial ecology. We transcriptionally profiled vegetative cells of M. xanthus strains possessing vs lacking pxr and found that over half of the genes impacted by pxr deletion during growth are linked to development, including known and potentially novel critical regulators. Many other genes are associated with general metabolic processes, which Pxr regulates positively. Our study discovers new phenotypic effects of Pxr regulation of likely ecological importance, identifies the suite of genes this sRNA controls during vegetative growth, reveals a previously unknown developmental regulator and provides new insights into the early molecular regulation of myxobacterial development.

opencc-zeroMay 2024View details →

opencc-by-4.0May 2024View details →

Supplementary material for "Development of novel carbon-free cobalt-free iron-based hardfacing alloys with a hard π-ferrosilicide phase"

EBSD, EDS and XRD data for the manuscript "<a href="https://www.sciencedirect.com/science/article/pii/S2589152924001042">Development of novel carbon-free cobalt-free iron-based hardfacing alloys with a hard π-ferrosilicide phase</a>"

opencc-zeroMay 2024View details →

FIG. 1 in Microsatellite development in the freshwater red alga Batrachospermum gelatinosum (L.) De Candolle (Batrachospermales, Rhodophyta)

FIG. 1. — Images of Batrachospermum gelatinosum (L.) De Candolle: A, gametophytes fixed to a log at Cedar Bog in Ohio. Often this species has a brown to olive-green color; B, multiple gametophytes in a 55 cm diameter bowl;C, a single gametophyte mounted on herbarium paper. Photo credits: A, Stacy A. KruegerHadfield; B, C, Morgan L. Vis. Scale bars: A, 10 cm; B, 1.5 cm; C, 1 cm.

opencc-by-4.0Jan 2014View details →

Рис. 2. Жан-Батист Ламарк в период написания «Естественной истории беспоЗвоночных» (источник: Wikimedia Commons). in Contribution of Georges Cuvier and Jean-Baptiste de Lamarck to development of the system of Mollusca

Рис. 2. Жан-Батист Ламарк в период написания «Естественной истории беспоЗвоночных» (источник: Wikimedia Commons).

opencc-by-4.0Dec 2017View details →

Fig. 1–3 in A new species of Eimeria Schneider, 1875 from the Serra dos Órgãos National Park, Rio de Janeiro, Brazil, with notes on its endogenous development in the montane grass mouse, Akodon montensis Thomas, 1913 (Rodentia: Sigmodontinae)

Fig. 1–3 Nomarski interference-contrast photomicrographs of Eimeria akodonensis n. sp. from the montane grass mouse, Akodon montensis. Presence of oocyst residuum (Or) and highly refractile polar granule (Pg); Sporocyst residuum (Sr); wall (Ow); Stieda body (Sb); sporozoite (Sp). Scale bar = 10 μm

opencc-by-4.0Dec 2017View details →

Fig. 5–12 in A new species of Eimeria Schneider, 1875 from the Serra dos Órgãos National Park, Rio de Janeiro, Brazil, with notes on its endogenous development in the montane grass mouse, Akodon montensis Thomas, 1913 (Rodentia: Sigmodontinae)

Fig. 5–12 Light micrographs of the endogenous stages of Eimeria akodonensis n. sp. observed in the lamina propria of the small intestine of Akodon montensis, showing the microgamonts (Mi) and the parasitophorous vacuole in the host cell (Vp); microgametes (Mi); macrogamonts (Ma); nucleus (N) and the zygote (Zy) with wallforming bodies arranged around its periphery (Ow); oocyst (Oo). Scale bar = 10 μm

opencc-by-4.0Dec 2017View details →

Fig. 4 in A new species of Eimeria Schneider, 1875 from the Serra dos Órgãos National Park, Rio de Janeiro, Brazil, with notes on its endogenous development in the montane grass mouse, Akodon montensis Thomas, 1913 (Rodentia: Sigmodontinae)

Fig. 4 Composite line drawing of the sporulated oocyst of Eimeria akodonensis n. sp. Scale bar = 10 μm

Fig 4 in Development of specific PCR assays for the detection of Cryptocaryon irritans

Fig 4 Agarose gel electrophoresis of PCR products amplified from the simulated water body using Cryptocaryon irritans-specific primer set P1/S15. Lanes 1 and 10 represent a DNA size marker, lanes 2 to 7 represent C. irritans from the infected water, lane 8 represents the uninfected water, and lane 9 represents no-DNA control

Fig 1 in Development of specific PCR assays for the detection of Cryptocaryon irritans

Fig 1 Agarose gel electrophoresis of PCR products amplified using conserved primer set P1/NC2 (a), Cryptocaryon irritans-specific primer set P1/S15 (b), and Ichthyophthirius multifiliis-specific primer set S01/S02 (c). Lanes 1 and 11 represent a DNA size marker. Lanes 2 to 9 represent C. irritans, Pseudokeroronpsis rubra, Pseudokeroronpsis carnae, Euplotes sp. 1, I. multifiliis, Pseudourostyla cristata, Paramecium caudaium (cf. Table 1), and host (fish) DNA, respectively. Lane 10 represents no-DNA control

Fig 2 in Development of specific PCR assays for the detection of Cryptocaryon irritans

Fig 2 Agarose gel electrophoresis of PCR products amplified from mixed genomic DNA of C. irritans and I. multifiliis. Lanes 1 and 7 represent a DNA size marker. Lane 2 presents the mixture of C. irritans and I. multifiliis DNA amplified using the primer set P1/S15 and lane 3 represents the genomic DNA of I. multifiliis. Lane 4 represents the mixed genomic DNA of C. irritans and I. multifiliis amplified using the primer set S01–S02. Lane 5 represents the genomic DNA of C. irritans using the primer set S01–S02. Lane 6 represents no-DNA control

Fig 3 in Development of specific PCR assays for the detection of Cryptocaryon irritans

Fig 3 Evaluation of the sensitivity of the specific PCR assay for Cryptocaryon irritans (primer set P1/S15) by agarose gel electrophoresis. Lanes 1 and 13 represent a DNA size marker. Lane 2 represents DNA undiluted (450 ng/µl), and lanes 3 to 11 represent C. irritans DNA diluted for 1:50 (9 ng/µl), 1:100 (4.5 ng/µl), 1:200 (2.25 ng/µl), 1:400 (1.13 ng/µl), 1:800 (0.56 ng/µl), 1:103 (0.45 ng/µl), 1:104 (45 pg/µl), 1:105 (4.5 pg/µl), and 1:106 (0.45 pg/µl), respectively. Lane 12 represents no-DNA control

Fig. 5 in Captive management, reproduction, and comparative larval development of Klappenbach's Red-bellied Frog, Melanophryniscus klappenbachi Prigioni and Langone, 2000

Fig. 5. Body size of different test groups. (A) Single tadpole, O‒1, and (B) five tadpoles per box, O‒5, in osmosis water. (C) Single tadpole, P‒1, and (D) five tadpoles per box, P‒5, in pond water.

Fig. 2. Keeping and rearing M in Captive management, reproduction, and comparative larval development of Klappenbach's Red-bellied Frog, Melanophryniscus klappenbachi Prigioni and Langone, 2000

Fig. 2. Keeping and rearing M. klappenbachi. (A) Terrarium of the adult group housing eight specimens. (B) Rearing of the tadpole test groups in a climate chamber.(C) Rearing containers for the young toadlets.

Fig. 1 in Captive management, reproduction, and comparative larval development of Klappenbach's Red-bellied Frog, Melanophryniscus klappenbachi Prigioni and Langone, 2000

Fig. 1. Melanophryniscus klappenbachi. (A) Dorsal and (B) ventral view of an adult female. (C) Amplexus.(D) Egg clump attached to moss. (E) Contrasting photo of a tadpole, used for evaluating the growth.

Fig. 4 in Captive management, reproduction, and comparative larval development of Klappenbach's Red-bellied Frog, Melanophryniscus klappenbachi Prigioni and Langone, 2000

Fig. 4. (A) Mortality rate of different test groups until metamorphosis. (B) Average growth rate of the different test groups. (C) Number of tadpoles metamorphosed per day after hatching (O = osmosis water, P = pond water, number indicates individuals per container).