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293 results for “Clamping”
Recurrent mismatch binding by MutS mobile clamps on DNA localizes repair complexes nearby
<p>DNA mismatch repair (MMR), the guardian of the genome, commences when MutS identifies a mismatch and recruits MutL to nick the error-containing strand, allowing excision and DNA resynthesis. Dominant MMR models posit that after mismatch recognition, ATP converts MutS to a hydrolysis-independent, diffusive mobile clamp that no longer recognizes the mismatch. Little is known about the postrecognition MutS mobile clamp and its interactions with MutL. Two disparate frameworks have been proposed: One in which MutS–MutL complexes remain mobile on the DNA, and one in which MutL stops MutS movement. Here we use single-molecule FRET to follow the postrecognition states of MutS and the impact of MutL on its properties. In contrast to current thinking, we find that after the initial mobile clamp formation event, MutS undergoes frequent cycles of mismatch rebinding and mobile clamp reformation without releasing DNA. Notably, ATP hydrolysis is required to alter the conformation of MutS such that it can recognize the mismatch again instead of bypassing it; thus, ATP hydrolysis licenses the MutS mobile clamp to rebind the mismatch. Moreover, interaction with MutL can both trap MutS at the mismatch en route to mobile clamp formation and stop movement of the mobile clamp on DNA. MutS's frequent rebinding of the mismatch, which increases its residence time in the vicinity of the mismatch, coupled with MutL's ability to trap MutS, should increase the probability that MutS–MutL MMR initiation complexes localize near the mismatch.</p> <p> </p>
Data from: Duration of cord clamping and neonatal outcomes in very preterm infants
Background: Delayed cord clamping (DCC, ≥30s) increases blood volume in newborns and is associated with fewer blood transfusions and short-term neonatal complications. The optimal timing of cord clamping for very preterm infants should maximize placental transfusion without interfering with stabilization and resuscitation. Aim: We compared the effect of different durations of DCC, 30-45s vs. 60-75s, on delivery room (DR) and neonatal outcomes in preterm infants <32 weeks gestational age (GA). Methods: This is a single-center prospective observational study. Data were collected prospectively from eligible infants from two groups: 30-45s DCC group (January 2008 to February 2011, n = 187) and 60-75s DCC group (March 2011 to April 2014, n = 166). Results: The 60-75s DCC group compared to the 30-45s DCC group had higher hematocrits at <2 hours (49.2% vs. 47.4%, p = 0.02). In infants <28 weeks GA, the 12–36 hours hematocrit was higher in the 60-75s DCC group compared to the 30-45s DCC group (47.9% vs. 42.1%, p = 0.002). The 60-75s DCC group had reductions in DR intubation (11% vs. 22%, p = 0.004), hypothermia on admission (1% vs. 5%, p = 0.01), surfactant therapy (13% vs. 28%, p = 0.001), intubation in the first 24 hours (20% vs. 34%, p = 0.004), any intubation (27% vs. 40%, p = 0.007), and any red blood cell transfusion (20% vs. 33%, p = 0.008) during the hospitalization compared to the 30-45s DCC group. These reductions remained significant after adjusting for GA, gender and >48 hours of antenatal steroid exposure. There was no difference between the two groups in neonatal death, intraventricular hemorrhage, chronic lung disease, late onset sepsis, necrotizing enterocolitis and severe retinopathy of prematurity. Conclusion: In this study cohort increasing DCC duration from 30-45s to 60-75s is associated with decreased hypothermia on admission, neonatal respiratory interventions and red blood cell transfusions without increase in neonatal mortality and morbidities.
FIGURE. Microscopic structures of Humidicutis roseorubra (KaiR621, holotype). a. Pileipellis and upper pileitrama. b. Basidia with toruloid clamps at different developmental stages. c. Basidiospores. Bars a = 20 µm, b, c = 10 µm. Drawings by K. Reschke. in New and interesting species of Agaricomycetes from Panama
FIGURE. Microscopic structures of Humidicutis roseorubra (KaiR621, holotype). a. Pileipellis and upper pileitrama. b. Basidia with toruloid clamps at different developmental stages. c. Basidiospores. Bars a = 20 µm, b, c = 10 µm. Drawings by K. Reschke.
Patch-clamp dataset (SyncroPatch384PE) for control variants used in a calibrated functional patch clamp assay to enhance clinical variant interpretation in KCNH2-related long QT syndrome
<p><span>Modern sequencing technologies have revolutionised our detection of gene variants. However, in most genes, including <em>KCNH2</em>, the majority of missense variants are currently classified as variants of uncertain significance (VUS). The aim of this study </span><span class="normaltextrun"><span>was to investigate the utility of an automated patch-clamp assay for aiding clinical variant classification in the <em>KCNH2 </em>gene. </span></span><span>The assay was designed according to recommendations proposed by the ClinGen Sequence Variant Interpretation Working Group. Thirty-one variants (17 pathogenic/likely pathogenic, 14 benign/likely benign) were classified internally as variant controls. They were heterozygously expressed in Flp-In HEK293 cells for assessing the effects of variant on current density and channel gating in order to determine the sensitive and specificity of the assay. All 17 pathogenic variant controls had reduced current density and 13/14 benign variant controls had normal current density, which enabled determination of normal and abnormal ranges for applying moderate or supporting evidence strength for VUS reclassification. Inclusion of <em>KCNH2</em> functional assay evidence enabled us to reclassify 6 out of 44 VUS as likely pathogenic. The high-throughput patch clamp assay can provide moderate strength evidence for clinical interpretation of clinical <em>KCNH2</em> variants and </span><span>demonstrates the value of developing automated patch clamp assays for functional characterisation of ion channel gene variants.</span></p>
ASDF: Assembly State Detection Utilizing Late Fusion by Integrating 6D Pose Estimation - Training Set - Corner Clamp Part 2 - Geared Caliper Base
Open the record for dataset details and reuse information.
Patch clamp recording from rat hippocampal neuron culture using bulk and NEA chip
<p>Patch-clamp, in current-clamp mode, recordings from rat hippocampal neuron culture using stimulated nano electrode array chip in bulk and low-density NEA chip. All the uncompressed data are in easy-to-use .mat files divided for type of chip (Bulk and NEA); type of coating material and type of stimulation (only for Bulk).</p>
ASDF: Assembly State Detection Utilizing Late Fusion by Integrating 6D Pose Estimation - Training Set - Hand Screw Clamp
<p>@article{schieber2024asdf,<br> title={ASDF: Assembly State Detection Utilizing Late Fusion by Integrating 6D Pose Estimation},<br> author={Schieber, Hannah and Li, Shiyu and Corell, Niklas and Beckerle, Philipp and Kreimeier, Julian and Roth, Daniel},<br> journal={arXiv preprint arXiv:2403.16400},<br> year={2024}<br>}</p>
FIGURE 4. Wrightoporia bracei. A. Basidiospores. B. Generative hyphae with verticillate clamps. C. Simple septate generative hyphae. D. Skeletal hyphae. E in Studies on Wrightoporia (Basidiomycota) from southern Brazil
FIGURE 4. Wrightoporia bracei. A. Basidiospores. B. Generative hyphae with verticillate clamps. C. Simple septate generative hyphae. D. Skeletal hyphae. E. Gloeopleurous hyphae. Illustrations by M.A. Reck.
Original recordings from voltage clamp fluorometry analyses of ANAP-containing P2X7 mutants
<p><span>The large intracellular C-terminus of the pro-inflammatory P2X7 ion channel receptor (P2X7R) is associated with diverse P2X7R-specific functions. Cryo-EM structures of the closed and ATP-bound open full-length P2X7R recently identified a membrane-associated anchoring domain, an open-state stabilizing "cap" domain, and a globular "ballast domain" containing GTP/GDP and dinuclear Zn<sup>2+</sup>-binding sites with unknown functions. To investigate protein dynamics during channel activation, we improved incorporation of the environment-sensitive fluorescent unnatural amino acid L-3-(6-acetylnaphthalen-2-ylamino)-2-aminopropanoic acid (ANAP) into <em>Xenopus</em> <em>laevis</em> oocyte-expressed P2X7Rs and performed voltage clamp fluorometry (VCF). While we confirmed predicted conformational changes within the extracellular and the transmembrane domains, only three out of 41 mutants containing ANAP in the C-terminal domain resulted in ATP-induced fluorescence changes. We conclude that the ballast domain functions rather independently from the extracellular ATP binding domain and might require activation by additional ligands and/or protein interactions. Novel tools to study these are presented.</span></p>
The Hematologic Impact of Umbilical Cord Milking Versus Deferred Cord Clamping in Premature Neonates.
ClinicalTrials.gov study NCT03147846. IPD Sharing: NO. Countries: 1. Publications: 1.
VentFirst: A Multicenter RCT of Assisted Ventilation During Delayed Cord Clamping for Extremely Preterm Infants
ClinicalTrials.gov study NCT02742454. IPD Sharing: NO. Countries: 2. Publications: 2.
Delayed Cord Clamping and Use of Oxytocin
ClinicalTrials.gov study NCT02618499. IPD Sharing: NO. Countries: 1. Publications: 10.
The Effect of Percutaneous Superior Venae Cava Cannulation Clamping on Cerebral Near Infrared Spectroscopy in MICS
ClinicalTrials.gov study NCT01166841. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Delayed Umbilical Cord Clamping - C-section Pilot
ClinicalTrials.gov study NCT02229162. IPD Sharing: NO. Countries: 1. Publications: 1.
Dynamics of Insulin Absorption in Subclinical Lipohypertrophy Using the Euglycemic Clamp Technique
ClinicalTrials.gov study NCT03229850. IPD Sharing: NO. Countries: 1. Publications: 12.
Tissue Oxygenation of the Tibial Anterior Muscle After Clamping of the Femoral Artery
ClinicalTrials.gov study NCT02038062. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Effect of Delayed Cord Clamping in Preterm Neonates With Placental Insufficiency
ClinicalTrials.gov study NCT03731546. IPD Sharing: NO. Countries: 1. Publications: 3.
A Trial to Compare Pringle Maneuver With Either Infrahepatic Inferior Vena Cava Clamping or Low Central Venous Pressure
ClinicalTrials.gov study NCT01355887. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Effects of Delayed Cord Clamping in Very Low Birth Weight Infants
ClinicalTrials.gov study NCT00840983. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Umbilical Cord Clamping and Infant Iron Status
ClinicalTrials.gov study NCT00298051. IPD Sharing: Not stated. Countries: 1. Publications: 2.
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.