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Fig. 3 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS/MS data of reference desulfoglucosinolates
Fig. 3. HPLC-MS chromatograms of desulfoglucosinolates (dGSLs) prepared from glucosinolates (GSLs) in Planodes virginica (A) and Nasturtium officinale (B–C) seeds, showing qualitative similarities and quantitative contrasts. Major peaks (B) from N. officinale revealed many of the same GSLs as in A, but levels of EBAR (40R) were much lower while levels were much higher for the biosynthetic precursor PE (105). A focus on trace peaks from N. officinale (C) revealed sharp peaks representing a range of minor constituents. Due to the closely eluting peaks, the latter chromatograms (B–C) were made by combining extracted ion chromatograms corresponding to [M+Na]+ of the indicated dGSLs. In C, the m/z 366 signal of d105 was omitted to allow visualization of minor coeluting peaks. An asterisk after a peak number indicates tentative identification. HPLC-MS conditions as in Olsen et al. (2016). TIC, total ion chromatogram, EIC, extracted ion chromatogram.
Fig. 4 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS/MS data of reference desulfoglucosinolates
Fig. 4. HPLC-MS chromatogram of desulfoglucosinolates (dGSLs) prepared from glucosinolates (GSLs) from leaves (A) and petioles (B) of horseradish (Armoracia rusticana), focusing on trace level GSLs. The chromatograms were made by combining extracted ion chromatograms corresponding to [M+Na]+ of the indicated dGSLs, from analyses that were much overloaded with respect to the dominating dGSL d107 from Pren. In panel A, an insert shows magnification of the chromatogram from 5.2 to 5.8 min. Neither suggested BAR nor EBAR were detectable. In panel B, only extracted ion chromatograms of m/z 382 (BAR/EBAR), 352 (BZ), 366 (PE), 380 (3PP), 394 (4PB), 408 (5PP at high tR and 6mSOh at 5.4 min), 422 (7mSOh) and 436 (8mSOo) are included. Unlabeled trace peaks did not exhibit a combination of tR and m/z suitable for any of the mentioned candidates. HPLC conditions as in Olsen et al. (2016). Panel A depicts analysis of the Copenhagen garden accession; panel B from the naturalized population at Lake Fures¨o. An asterisk after a peak number indicates tentative identification.
Fig. 2 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS/MS data of reference desulfoglucosinolates
Fig. 2. Detection of thioglucose-acylated glucosinolates (GSLs) by HPLC-MS of desulfated derivatives prepared from the indicated species. (A–C) Analysis of seeds of Arabidopsis thaliana Col-0 used as reference material for characteristic GSLs. Shown are the total ion chromatogram (A) and extracted ion chromatograms for sodium adducts of desulfo 6′Bz 4BzOb (d125) (B) and desulfo 6′Bz 4mSb (d127) (C). (D–F) Analysis of seeds of Barbarea grayi for dominating GSLs. Shown are total ion chromatograms (D), and extracted ion chromatograms for sodium adducts of desulfo 6'iF BAR (d131S) (E) and desulfo 6'iF PE (d129) (F).
Fig. 1 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS/MS data of reference desulfoglucosinolates
Fig. 1. MS2 spectra of pairs of desulfoglucosinolates with and without a side chain double bond. Four short chain desulfoglucosinolates were investigated, including Na+ adducts of all (A–D) and in addition H+ adducts of the methylthio substituted (E–F), as indicated in each spectrum. The desulfo derivative of the putative 9mSn ([89]), poorly characterized in the literature, was also investigated (G).
Fig. 5. Proposed fragmentation scheme for 2-aminobenzoyl O in Chemotaxonomic investigation of Apocynaceae for retronecine-type pyrrolizidine alkaloids using HPLC-MS/MS
Fig. 5. Proposed fragmentation scheme for 2-aminobenzoyl O-β-D-apiofuranosyl-(1 → 6)- β-D-glucopyranoside (m/z 432.15 when protonated) in positive ion mode ESI to m/z 300.11, 138.06, and 120.04 fragments.
Fig. 2 in Chemotaxonomic investigation of Apocynaceae for retronecine-type pyrrolizidine alkaloids using HPLC-MS/MS
Fig. 2. Common esterification patterns between necic acids and necine bases observed in retronecine-type PAs.
Factorial design assisted RP-HPLC method for simultaneous determination of fluconazole, itraconazole, and terbinafine
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High Performance Liquid Chromatography (HPLC) pigment analysis from rosette bottle samples at various depths from CCE LTER process cruises in the California Current System, 2006 to 2017.
High Performance Liquid Chromatography (HPLC) samples are collected from rosette bottles (from three to eight different depths in the photic zone) at stations located within the CCE region on Process cruises (since 2006, ongoing). The HPLC method is used to measure concentrations of chlorophylls and carotenoids in samples of particulate matter, which includes filtering and freezing the filter while at sea. The taxon-specific phyto-pigments are extracted back onshore. Concentrations of chlorophyll a are used as a proxy for phytoplankton biomass and concentrations of other taxon-specific pigments are used to determine contributions of phytoplankton taxa to total phytoplankton biomass.
Figure 2 from: Kyslychenko OA, Protska VV, Zhuravel IO (2019) HPLC determination of phenolic compounds content in Parmelia sulcata and Parmelia vagans thalli. Pharmacia 66(4): 161-164. https://doi.org/10.3897/pharmacia.66.e35194
Figure 2 The HPLC chromatograms of Parmelia vagans thalli under the conditions of positive (A) and negative (B) ionization.
Figure 1 from: Kyslychenko OA, Protska VV, Zhuravel IO (2019) HPLC determination of phenolic compounds content in Parmelia sulcata and Parmelia vagans thalli. Pharmacia 66(4): 161-164. https://doi.org/10.3897/pharmacia.66.e35194
Figure 1 The HPLC chromatograms of Parmelia sulcata thalli under the conditions of positive (A) and negative (B) ionization.
Figure 4 from: Drapak I, Zimenkovsky B, Ivanauskas L, Bezruk I, Perekhoda L, Muzychenko V, Logoyda L, Demchuk I (2020) HPLC method for simultaneous determination of impurities and degradation products in Cardiazol. Pharmacia 67(1): 29-37. https://doi.org/10.3897/pharmacia.67.e37004
Figure 4 Chromatogram of a resolution mixture (impurity B (12.04; 0.65); impurity B (18.5; 0.98); Cardiazol (18.87; 1.00).
Gradients1-KOK1606-HPLC_2020-03-23_v1.2
<p>Cruise: Gradients 1, KOK1606</p> <p>Project Name: Simons Foundation, Gradients NPSG</p> <p>Method: Samples were processed to detect and quantify phytoplankton pigments using a modified version of the high-performance liquid chromatography (HPLC) method of Wright et al. (1991) as described by Bidigare et al. (2005). Sample water was concentrated onto Whatman GF/F filters (0.7 µm nominal pore size) and stored at -80°C until analysis. Filters were extracted in 100% acetone for 24 h at 4°C. Extracts were processed in reverse-phase on a 250 mm C18 column using a Waters separations module and photodiode detector array. Empower chromatography software was used for integration and pigment quantification. The instrument was calibrated using chlorophyll a from Anacystis nidulans (Sigma USA) and mixed pigment standards were obtained from DHI in Denmark. </p>
Figure 2 from: Logoyda L (2020) Efficient validated method of HPLC to determine amlodipine in combinated dosage form containing amlodipine, enalapril and bisoprolol and in vitro dissolution studies with in vitro/ in vivo correlation. Pharmacia 67(2): 55-61. https://doi.org/10.3897/pharmacia.67.e48220
Figure 2 Representative chromatogram of amlodipine in combinated tablets (1- peak of bisoprolol, 2- peak of enalapril, 3 – peak of amlodipine).
Figure 6 from: Sharmin S, Sohrab MH, Moni F, Afroz F, Rony SR, Akhter S (2020) Simple RP-HPLC method for Aceclofenac quantitative analysis in pharmaceutical tablets. Pharmacia 67(4): 383-391. https://doi.org/10.3897/pharmacia.67.e57981
Figure 6 Robustness Study (a) Variance of peak area for change in different method parameters with %RSD; (b) Variance of Retention time for change in different method parameters with %RSD. *Method was robust for change in pH of mobile phase (±0.5), wavelength (±3 nm) and column temperature (±3 °C).
Figure 4 from: Logoyda L (2021) Novel HPLC-UV method for simultaneous determination of valsartan and atenolol in fixed dosage form; Study of green profile assessment. Pharmacia 68(1): 43-51. https://doi.org/10.3897/pharmacia.68.e53631
Figure 4 Chromatogram obtained using Shimadzu Nexera XR UPLC system and mobile phase 20% acetonitrile, 80% of 0.16% ammonium acetate and 0.2% of 1.5 M tetramethylammonium hydroxide (V/V), column Discovery C18 (4.6 mm i.d. × 150 mm, 5 μm) at 2 wavelengths 225 nm and 237 nm (first figure), with 3-D UV contour diagram extracted analytes UV spectra and peak purity (second figure).
Figure 6 from: Logoyda L (2021) Novel HPLC-UV method for simultaneous determination of valsartan and atenolol in fixed dosage form; Study of green profile assessment. Pharmacia 68(1): 43-51. https://doi.org/10.3897/pharmacia.68.e53631
Figure 6 Chromatogram obtained using final established, optimized and validated chromatographic method at wavelength 237 nm.
Supplementary material 1 from: Logoyda L (2021) Novel HPLC-UV method for simultaneous determination of valsartan and atenolol in fixed dosage form; Study of green profile assessment. Pharmacia 68(1): 43-51. https://doi.org/10.3897/pharmacia.68.e53631
Supplementary data
Figure 3 from: Logoyda L (2021) Novel HPLC-UV method for simultaneous determination of valsartan and atenolol in fixed dosage form; Study of green profile assessment. Pharmacia 68(1): 43-51. https://doi.org/10.3897/pharmacia.68.e53631
Figure 3 Elution profiles obtained for test samples prepared of Valsartan + Atenolol tablets (80 + 100) mg using mobile phases: a) 20% acetonitrile and 80% of 0.16% ammonium acetate (V/V); b) 20% acetonitrile, 80% of 0.16% ammonium acetate and 0.2 % of 1.5 M tetramethylammonium hydroxide (V/V). Chromatographic conditions: Shimadzu Nexera XR UPLC system, C18 chromatographic column Discovery C18 (4.6 mm i.d. × 150 mm, 5 μm), flow rate 1.0 mL/min, column temperature 30 °C.
Figure 5 from: Logoyda L (2021) Novel HPLC-UV method for simultaneous determination of valsartan and atenolol in fixed dosage form; Study of green profile assessment. Pharmacia 68(1): 43-51. https://doi.org/10.3897/pharmacia.68.e53631
Figure 5 Chromatogram obtained using final established, optimized and validated chromatographic method at wavelength 225 nm.
Figure 7 from: Piponski M, Balkanov T, Logoyda L (2021) Development and validation of a fast and simple HPLC method for the simultaneous determination of bisoprolol and enalapril in dosage form. Pharmacia 68(1): 69-77. https://doi.org/10.3897/pharmacia.68.e50919
Figure 7 Linearity illustrating chromatograms obtained using final established, optimized and validated chromatographic method, with 55% methanol. First peak is bisoprolol and second enalapril Dionex Ultimate 3000 UHPLC system.
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