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150 results for “chitosan”
MicroFIBERgut: Effects of Lifestyle Changes and Chitosan on Gut Microbiota and Weight Management
ClinicalTrials.gov study NCT04551365. IPD Sharing: YES. Countries: 1. Publications: 1.
A Crossover Trial of Chitosan Oligosaccharide on Post Prandial Glucose Control in Subjects With Normal, IFG and IGT
ClinicalTrials.gov study NCT03650023. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Resveratrol loaded chitosan-pectin core-shell nanoparticles as novel drug delivery vehicle for sustained release and improved antioxidant activities
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Data from: Niclosamide loaded biodegradable chitosan nanocargoes: an in vitro study for potential application in cancer therapy
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Magnetic transfection with superparamagnetic chitosan-loaded IGFBP5 nanoparticles and their in vitro biosafety
<p><strong>Purpose: </strong>To study the application of superparamagnetic chitosan nanoparticles (SPCIONPs) as gene vectors using a magnetic transfection system for the targeted treatment of lung metastasis of osteosarcoma.</p> <p><strong>Methods: </strong>The superparamagnetic chitosan nanoparticles were characterized by Transmission Electron Microscopy, Fourier Transform Infrared spectrometry, a Superconducting Quantum Interference Device and Atomic Force Microscopy. Their biosafety was determined by cell counting kit-8 (CCK8) and live-dead staining assays. In vitro transfection was detected by laser confocal microscopy.</p> <p><strong>Results:</strong> SPCIONPs, which can bind closely to plasmids and protect them from DNA enzyme degradation, were prepared with an average particle size of 95.60 nm and zeta potential of 11 mV. The results of the CCK8 and live-dead staining assays showed that superparamagnetic chitosan nanoparticles loaded with Insulin-like growth factor-binding protein 5 (SPCIONPs/pIGFBP5) induced no significant cytotoxicity compared to the control group. The in vitro transfection result suggested that pIGFBP5 emitted a greater amount of red fluorescence in the SPCIONPs/pIGFBP5 group than that in the chitosan-loaded IGFBP5 (CS/pIGFBP5) group.</p> <p><strong>Conclusion: </strong>The prepared SPCIONPs had good biosafety and could be effectively used to transfer pIGFBP5 into 143B cells, and they thus have good application prospects for the treatment of lung metastasis of osteosarcoma.</p>
Supplementary material 1 from: Sadaqa E, Utami RA, Mudhakir D (2024) In vitro cytotoxic and genotoxic effects of Phyllanthus niruri extract loaded chitosan nanoparticles in TM4 cells and their influence on spermatogenesis. Pharmacia 71: 1-14. https://doi.org/10.3897/pharmacia.71.e112138
Supplementary information
Figure 7 from: Sadaqa E, Utami RA, Mudhakir D (2024) In vitro cytotoxic and genotoxic effects of Phyllanthus niruri extract loaded chitosan nanoparticles in TM4 cells and their influence on spermatogenesis. Pharmacia 71: 1-14. https://doi.org/10.3897/pharmacia.71.e112138
Figure 7 Imaging of cellular morphological change in the presence of ChNP and PNNP. TM4 cells were incubated in the absence of nanoparticles (a) and in the presence of ChNP at 50 µg/mL (b), PNNP at 125 µg/mL (c), and ChNP at 200 µg/mL (d). Images were captured 2 h after transfection using a 20× lens on a CLSM (Olympus FV-1200) and the nucleus was stained with Hoechst 33342 (blue). The bar indicates 50 µm.
Figure 9 from: Sadaqa E, Utami RA, Mudhakir D (2024) In vitro cytotoxic and genotoxic effects of Phyllanthus niruri extract loaded chitosan nanoparticles in TM4 cells and their influence on spermatogenesis. Pharmacia 71: 1-14. https://doi.org/10.3897/pharmacia.71.e112138
Figure 9 Claudin 11 expression in the presence of ChNP and PNNP. TM4 cells were incubated with ChNP 50 µg/mL, PNNP 125 µg/mL and ChNP 200 µg/mL for 24 h. a. Confocal images of claudin 11 expression after cells were incubated with a secondary antibody goat anti-rabbit IgG Alexa Fluor 488 (green) and the nucleus was stained with Hoechst 33342 (blue). The bars indicate 50 µm; b. Histogram of downregulation claudin 11 expression. The results are shown as mean ± SE of two separate studies (n = 50). ns (not significant) p > 0.05. Stars (***) indicate significance when compared with the control group (untreated cells) (p < 0.001). Pound signs (###) demonstrate significance compared with ChNP 50 µg/mL.
Figure 8 from: Sadaqa E, Utami RA, Mudhakir D (2024) In vitro cytotoxic and genotoxic effects of Phyllanthus niruri extract loaded chitosan nanoparticles in TM4 cells and their influence on spermatogenesis. Pharmacia 71: 1-14. https://doi.org/10.3897/pharmacia.71.e112138
Figure 8 Connexin 43 expression in the presence of ChNP and PNNP. TM4 cells were incubated with ChNP 50 µg/mL, PNNP 125 µg/mL and with ChNP 200 µg/mL for 24 h. a. Confocal images of connexin 43 expression after cells were incubated with a secondary antibody goat anti-rabbit IgG Alexa Fluor 488 (green) and the nucleus was stained with Hoechst 33342 (blue). The bars indicate 50 µm. b. Histogram of downregulation of connexin 43 expression. The results are shown as mean ± SEM of two separate studies (n=50). ns (not significant) p > 0.05. Stars (***) indicate significance when compared with the control group (untreated cells) (p < 0.001). Pound signs (###) demonstrate significance compared with ChNP 50 µg/mL.
Figure 6 from: Sadaqa E, Utami RA, Mudhakir D (2024) In vitro cytotoxic and genotoxic effects of Phyllanthus niruri extract loaded chitosan nanoparticles in TM4 cells and their influence on spermatogenesis. Pharmacia 71: 1-14. https://doi.org/10.3897/pharmacia.71.e112138
Figure 6 DNA breakage caused by ChNP and PNNP using FHA. TM4 cells were incubated with various concentration of ChNP (25, 50, 200 µg/mL) and PNNP (62.5, 125, 500 µg/mL) for 2 h. Positive control 100 µm H2O2 was used and incubated for 15 min. Photomicrograph obtained by Inverted Microscope Olympus IX73. Single stranded DNA was stained with ethidium bromide of a. NEgative control (untreated cells); b. H2O2; c.ChNP 25 µg/mL; d.ChNP 50 µg/mL; e.ChNP 200 µg/mL; f.PNNP 62.5 µg/mL; g.PNNP 125 µg/mL; h.PNNP 500 µg/mL. The bar indicates 5 µm. i. Histogram showing DNA damage level using NDF from several randomly selected microscope images. The results are shown as mean ± SEM of three independent experiments (n=150). ns (not significant) p > 0.05. ***p < 0.001 when compared to the corresponding control group.
Figure 4 from: Sadaqa E, Utami RA, Mudhakir D (2024) In vitro cytotoxic and genotoxic effects of Phyllanthus niruri extract loaded chitosan nanoparticles in TM4 cells and their influence on spermatogenesis. Pharmacia 71: 1-14. https://doi.org/10.3897/pharmacia.71.e112138
Figure 4 Cell viability percentage after 24 hours of exposure to various concentrations of ChNP and PNNP assessed by CCK8 test. The values are presented as mean ± standard deviation (SD) (n = 3). All data were analyzed using One-way ANOVA. The stars indicate significant difference between control negative (untreated cells) and various treatment groups (P < 0.05 significantly different, p-value ≥ 0.05 was determined as non-significant (ns). A p-value score of between 0.01 and 0.05 was considered significant (*), between 0.01 and 0.001 as very significant (**), and < 0.001 as extremely significant (***).
Figure 3 from: Sadaqa E, Utami RA, Mudhakir D (2024) In vitro cytotoxic and genotoxic effects of Phyllanthus niruri extract loaded chitosan nanoparticles in TM4 cells and their influence on spermatogenesis. Pharmacia 71: 1-14. https://doi.org/10.3897/pharmacia.71.e112138
Figure 3 Illustration of the TGA, DTG, and DTA curves for A.ChNP and B.PNNP. DTA curves are represented in green, DTG curves in red, and TGA curves in blue.
Figure 5 from: Sadaqa E, Utami RA, Mudhakir D (2024) In vitro cytotoxic and genotoxic effects of Phyllanthus niruri extract loaded chitosan nanoparticles in TM4 cells and their influence on spermatogenesis. Pharmacia 71: 1-14. https://doi.org/10.3897/pharmacia.71.e112138
Figure 5 DNA damage caused by ChNP and PNNP using comet assay. TM4 cells were incubated with various concentrations of ChNP and PNNP for 2 h. Positive control 100 µm H2O2 was used and incubated for 15 min. Comet images demonstrating the degree of DNA damage on TM4 cells were captured using Inverted Microscope Olympus IX73. Single stranded DNA was stained with ethidium bromide. Comet images displayed untreated cells as control (a), H2O2-treated cells (b), and ChNP-treated cells at concentrations of 25 µg/mL (c), 50 µg/mL (d), and 200 µg/mL (e), as well as PNNP-treated cells at concentrations of 62.5 µg/mL (f), 125 µg/mL (g), and 500 µg/mL (h). The bar indicates 5 µm. Quantitative assessment of DNA damage was performed by measuring olive tail moment (i) and % tail DNA (j). The data are presented as the mean ± SEM of three independent trials (n=200), ns (not significant) (***p < 0.001, **p < 0.01, *p < 0.05 when compared to the corresponding control group).
Figure 1 from: Sadaqa E, Utami RA, Mudhakir D (2024) In vitro cytotoxic and genotoxic effects of Phyllanthus niruri extract loaded chitosan nanoparticles in TM4 cells and their influence on spermatogenesis. Pharmacia 71: 1-14. https://doi.org/10.3897/pharmacia.71.e112138
Figure 1 Transmission electron microscope (TEM) images showing morphology of A. unloaded ChNP compared to B.PNNP. The scale bar represents 100 nm.
Facile synthesis of eco-friendly alginate-chitosan bio-adsorbent for critical raw materials adsorption: A comprehensive study
<p>This dataset contains the raw data for the publication "Facile synthesis of eco-friendly alginate-chitosan bio-adsorbent for critical raw materials adsorption: A comprehensive study" by Fila et al, published in Journal of Environmental Management. The upload includes raw data of physicochemical characterizations of alginate-chitosan composite, including TG, ATR/FT-IR, SEM, and XPS analyses. </p>
Data from: Enhanced specific loss power of hematite-chitosan nanohybrid synthesized by hydrothermal method
<p>We used a hydrothermal technique for producing hematite (a-Fe2O3) nanoparticles that were then functionalized with chitosan. The prepared iron oxide (a-Fe2O3) nanoparticles were single-phase, according to XRD analysis. The presence of lattice fringes in the HRTEM image confirmed the crystalline nature of the a-Fe2O3. The samples were coated with chitosan and the coating was confirmed by the spectra of Fourier transform infrared (FTIR) analysis. The Mössbauer spectra reveal a mixed relaxation state, which is also supported by the PPMS study. A zero field cooled study revealed the existence of a Morin transition. The hydrodynamic diameter of the coated particles was measured using the dynamic light scattering technique (DLS) to be between 218 and 235 nm, with a polydispersity index ranging from 0.048 to 0.119. The zeta potential was +46.8 mV, which is appropriate for colloidal stability. Both the Vero and HeLa cell lines demonstrated viability incubated for 24 hrs. with the colloids of different concentrations. The maximum temperature, Tmax attained by the hematite-chitosan nanohybrid solution of 0.25 and 4 mg/ml — the lowest and highest concentration, was 42.9 and 48.3ºC, and the specific loss power, SLP was 501.6 and 35.53, which are remarkably high for the Mmax; 300K = 1.98 emu/g.</p>
Clinical Application of Mesenchymal Stem Cells Seeded in Chitosan Scaffold for Diabetic Foot Ulcers
ClinicalTrials.gov study NCT03259217. IPD Sharing: UNDECIDED. Countries: 0. Publications: 1.
Chitosan Dressings to Facilitate Safe Effective Debridement of Chronic Wounds & Minimize Wound Bacterial Re-colonization
ClinicalTrials.gov study NCT01035944. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Effectiveness and Safety of Early-Stage Amputation and External Herbs Chitosan for Diabetic Foot Ulcer
ClinicalTrials.gov study NCT02413086. IPD Sharing: Not stated. Countries: 0. Publications: 5.
Study of Chitosan for Pharmacologic Manipulation of AGE Levels in Prostate Cancer Patients
ClinicalTrials.gov study NCT03712371. IPD Sharing: NO. Countries: 1. Publications: 0.
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