Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
6,383
datasets available to search
ShareScore release 0.9.0
Dataset results
6,383 results for “isolates”
Isolate specific transcriptome changes exerted by isavuconazole treatment in Candida auris
GEO Series GSE275177. Candidozyma auris. 8 samples. Type: Expression profiling by high throughput sequencing.
Analysis of transcriptomes of thymic populations isolated from neonatal thymi
GEO Series GSE131180. Homo sapiens. 15 samples. Type: Expression profiling by array.
Altered mouse mammary gland gene expression and tumor growth following chronic social isolation
GEO Series GSE16110. Mus musculus. 16 samples. Type: Expression profiling by array.
A tomato fruit RNA extraction method that isolates mRNAs encoding secreted and endomembrane associated proteins
GEO Series GSE17222. Solanum lycopersicum. 3 samples. Type: Expression profiling by array.
Gene expression profiling of uterine stromal and epithelial cells isolated from Msx1Msx2 floxed and Msx1Msx2 ablated mice on day 4 of pregnancy
GEO Series GSE30969. Mus musculus. 4 samples. Type: Expression profiling by array.
In-vivo inhibition of Fgfr2b signalling in FACS-isolated AECs from embryonic mouse lungs from E16.5 to E18.5
GEO Series GSE115878. Mus musculus. 8 samples. Type: Expression profiling by array.
Dataset related to the article "Lesion Index: A Novel Guide in the Path of Successful Pulmonary Vein Isolation"
<p>This record contains raw data related to the article "Lesion Index: A Novel Guide in the Path of Successful Pulmonary Vein Isolation".</p> <p> </p> <p>ABSTRACT</p> <p><strong>Purpose: </strong>Previous studies indicate force time integral (FTI) as a radiofrequency (RF) lesion quality marker, while not considering power supply. Tacticath™ Quartz catheter provides Lesion index (LSI), a lesion quality marker derived by contact force (CF), power supply, and RF time combined. Our aim is to assess LSI and FTI correlation and a LSI-related cutoff of atrial fibrillation (AF) recurrences 12 months after pulmonary vein isolation (PVI).</p> <p><strong>Methods: </strong>We retrospectively enrolled 37 patients who underwent RF ablation using Tacticath™ Quartz catheter. AF recurrence rate was evaluated 3, 6, and 12 months after PVI procedure.</p> <p><strong>Results: </strong>AF recurrence was detected in 32% of patients. FTI mean value was significantly lower in left superior pulmonary vein (LSPV: 256 ± 86 gs vs 329 ± 117 gs, p = 0.05) and right inferior pulmonary vein (RIPV: 253 ± 128 gs vs 394 ± 123 gs p = 0.006) in patients with AF recurrences; no significant differences were found in right superior pulmonary vein (RSPV) and left inferior pulmonary vein (LIPV). LSI instead was significantly higher for all veins in patients without AF recurrences: LSPV (5.2 ± 0.7 vs 4.6 ± 0.8, p = 0.03), LIPV (5.0 ± 0.8 vs 4.5 ± 0.6, p = 0.04), RSPV (5.5 ± 0.6 vs 5.1 ± 0.6, p = 0.05), and RIPV (5.5 ± 0.7 vs 4.7 ± 0.8, p = 0.006). Receiver operator characteristic curve suggests 5.3 as LSI overall cutoff value predicting freedom from disease at 1-year follow-up.</p> <p><strong>Conclusions: </strong>Our preliminary data suggest that a LSI mean value higher than 5.3 can be considered a good predictor of AF freedom at 1-year follow-up.</p>
Dataset related to article "Evaluation of Semen Self-Sampling Yield Predictors and CTC Isolation by Multi-Color Flow Cytometry for Liquid Biopsy of Localized Prostate Cancer"
<p><strong>Abstract</strong></p><p>Liquid biopsy (LB) for prostate cancer (PCa) detection could represent an alternative to biopsy. Seminal fluid (SF) is a source of PCa-specific biomarkers, as 40% of ejaculate derives from the prostate. We tested the feasibility of an SF-based LB by evaluating the yield of semen self-sampling in a cohort of >750 patients with clinically localized PCa. The overall SF collection yield was 18.2% (39% when considering only compliant patients), with about a half of the patients (53.15%) not consenting to SF donation. Independent favorable predictors for SF collection were younger age and lower prostate volume. We implemented a protocol to enrich prostate-derived cells by multi-color flow cytometry and applied it on SF and urine samples from 100 patients. The number of prostate-enriched cells (SYTO-16+ PSMA+ CD45-) was variable, with higher numbers of cells isolated from SF than urine (<i>p</i> value < 0.001). Putative cancer cells (EpCAMhigh) were 2% of isolated cells in both specimens. The fraction of EpCAMhigh cells over prostate-enriched cells (PSMA+) significantly correlated with patient age in both semen and urine, but not with other clinical parameters, such as Gleason Score, ISUP, or TNM stage. Hence, enumeration of prostate-derived cells is not sufficient to guide PCa diagnosis; additional molecular analyses to detect patient-specific cancer lesions will be needed</p>
Dataset for the study "Antimicrobial susceptibility testing reveals reduced susceptibility to azithromycin and other antibiotics in Legionella pneumophila serogroup 1 isolates from Portugal"
<p>Dataset for the study "Antimicrobial susceptibility testing reveals reduced susceptibility to azithromycin and other antibiotics in <em>Legionella pneumophila</em> serogroup 1 isolates from Portugal"</p>
Organic factors positively mediate stability across multiple communities in isolated aquatic ecosystems
Open the record for dataset details and reuse information.
Dataset related to the article "Key characteristics for effective acute pulmonary vein isolation when using a novel cryoballoon technology: insights from the CHARISMA registry"
<p>This record contains raw data related to the article "Key characteristics for effective acute pulmonary vein isolation when using a novel cryoballoon technology: insights from the CHARISMA registry".</p> <p><em><strong>Abstract</strong></em></p> <p><strong>Purpose: </strong>A new cryoballoon (CB) technology (POLARx™; Boston Scientific) for pulmonary vein (PV) isolation in patients with atrial fibrillation (AF) has recently been introduced. The aim of this study was to evaluate procedural and biophysical parameters resulting in acute PV isolation when using this new CB.</p> <p><strong>Methods: </strong>We assessed the first 69 consecutive patients indicated for AF ablation who underwent PV isolation by means of a novel CB system. Procedural metrics were prospectively recorded.</p> <p><strong>Results: </strong>A total of 274 PVs were targeted in 69 patients. PV isolation was achieved in all patients by means of cryoablation alone. The median time to isolation (TTI) was 44 [31-68] s (median temperature at TTI = - 49 [- 53 to - 41] °C). The median duration of CB ablation was 180 [180 to 240] s. The median nadir temperature was - 56.0 [- 61 to - 52] °C, and the median thaw time to 0 °C was 18 [15-21] s. The median grade of PV occlusion was 4 [3 to 4]. On the basis of ROC analysis, we defined the following cut-off values for acute PV isolation: - 56 °C for nadir temperature (sensitivity = 73.3%, specificity = 64.6%, AUC = 0.716; positive predictive value = 88.1%), 30 s for TTT (60.2%, 53.3%, 0.578; 79.7%), thaw time > 17 s (65.3%, 70.0%, 0.709; 86.9%), and grade of PV occlusion = 4 (79.4%, 66.7%, 0.738; 88.5%). No major procedure-related adverse events were observed at 30-day post-procedure.</p> <p><strong>Conclusions: </strong>The new POLARx™ CB appears to be effective and safe. A nadir temperature of - 56 °C, a thaw time to 0 °C ≥ 17 s, and complete PV occlusion were the best predictors of acute PV isolation.</p> <p><strong>Clinical trial registration: </strong>Catheter Ablation of Arrhythmias with a High-Density Mapping System in Real-World Practice (CHARISMA). URL: http://clinicaltrials.gov/Identifier : <a href="http://clinicaltrials.gov/show/NCT03793998">NCT03793998</a>. Registration date: January 4, 2019.</p>
Dataset related to the article "Cryoablation for pulmonary veins isolation in obese patients with atrial fibrillation compared to nonobese patients"
<p>This record contains raw data related to the article "Cryoablation for pulmonary veins isolation in obese patients with atrial fibrillation compared to nonobese patients"</p> <p><em><strong>Abstract</strong></em></p> <p><strong>Background: </strong>Pulmonary veins isolation (PVI) by cryoballoon (CB) ablation is marginally represented in clinical studies in obese patients. The aim of this analysis was to evaluate the safety and efficacy of CB-PVI in a large cohort of overweight and obese patients from the 1STOP project.</p> <p><strong>Methods: </strong>From 2012 to 2018, 2048 patients with atrial fibrillation (AF) (70% male, 59 ± 11 years; 75% paroxysmal AF) underwent index CB-PVI. The patient data were separated into three cohorts for statistical evaluation, including: normal weight (body mass index [BMI] < 25 kg/m<sup>2</sup> ), overweight (BMI = 25-30 kg/m<sup>2</sup> ), and obese patients (BMI > 30 kg/m<sup>2</sup> ).</p> <p><strong>Results: </strong>Out of 2048 patients, 693 (34%) patients had a BMI < 25 and were deemed as normal. There were 944 (46%) patients categorized as overweight (BMI = 25-30) and 411 (20%) as obese (BMI > 30). Overweight or obese patients were more often in persistent AF, had more frequently hypertension and diabetes, had higher CHA<sub>2</sub> DS<sub>2</sub> -VASc score, and had a number of failed antiarrhythmic drug (AAD). Periprocedural complication rates were similar among the three cohorts. The 12-month freedom from AF recurrence was 76.4% in the normal BMI group as compared to 79.2% in the overweight and 73.5% in the obese group (p = .35). However, 48% of overweight patients were on AAD treatment during the follow-up. By multivariate analysis, BMI was not a predictor for AF recurrence following the index CB-PVI.</p> <p><strong>Conclusion: </strong>CB-PVI in obese patients is a safe procedure. Increased BMI (either moderate or severe) does not seem to be associated with a worse outcome or to a different rate of AAD discontinuation at 12 months.</p> <p> </p> <p> </p>
Supplementary Table 1. Isolate code, geographic origin, year, host, phenotypic reaction on four differential wheat lines of Pyrenophora tritici-repentis, PCR reaction and race designation of Ptr isolates in Kazakhstan
<p>Supplementary Table 1. Isolate code, geographic origin, year, host, phenotypic reaction on four differential wheat lines of Pyrenophora tritici-repentis, PCR reaction and race designation of Ptr isolates in Kazakhstan</p>
Automated Isolation for White-Box Testing
<p>Evaluation dataset and analysis script used in our paper named Automated Isolation for White-Box Testing.</p>
Effect of antibiotic-loaded chitosan nanodroplets on Enterococci isolated from chronic ulcers of the lower limbs
<p>Dataset from the article Mazzaccaro D, Ticozzi R, D'Alessandro S, Delbue S, Nano G, Costa E, Argenziano M, Cavalli R, Prato M, Basilico N. Effect of antibiotic-loaded chitosan nanodroplets on Enterococci isolated from chronic ulcers of the lower limbs. Future Microbiol. 2020 Sep;15:1227-1236. doi: 10.2217/fmb-2019-0255. PMID: 33026879.</p> <p><strong>Abstract </strong></p> <p><strong>Aim:</strong> To investigate the effect of a new platform of nanocarriers, called nanodroplets (NDs), to enhance the <em>in vitro</em> activity of vancomycin (Vm), against bacterial colonies isolated from chronic ulcers of the lower limbs. <strong>Materials & methods:</strong> Oxygen-loaded nanodroplets (OLNDs) or oxygen-free nanodroplets (OFNDs) were loaded with Vm (Vm-OLNDs and Vm-OFNDs). MIC and minimal bactericidal concentrations were evaluated for Vm, OLNDs and OFNDs loaded with Vm, OLNDs and OFNDs. <strong>Results & conclusion:</strong> Nanodroplets, either with or without oxygen, appeared as a suitable platform of antibiotic nanocarriers to enhance the antibacterial effects of Vm against <em>Enterococci</em>, with a decrease in both MIC and minimal bactericidal concentration against Vm-resistant <em>Enterococci</em> strains.</p>
Dataset related to article: "The impact of the isolation protocol on the release of extracellular vesicles by human platelets"
<p>Dataset related to article: "The impact of the isolation protocol on the release of extracellular vesicles by human platelets"</p> <p>Background: Platelet-derived extracellular vesicles (PEVs) are small vesicles released by activated platelets that are gaining growing interest in the field of vascular biology. The mode of platelet activation is a critical determinant of PEVs release, phenotype and function. However, only very limited information is available concerning the impact of the platelet purification procedure on PEVs release. Methods: Washed or isolated platelets were separated by differential centrifugations. For washed platelets, the platelet pellet was washed by resuspension in PIPES buffer and finally resuspended in HEPES buffer. Isolated platelets were obtained by directly resuspending the platelet pellet in HEPES, skipping the washing steps in PIPES buffer. PEVs release was induced in washed or isolated platelets by stimulation with different agonist and analysed by Nanoparticle Tracking Analysis. Results: Isolated platelets showed a higher release of PEVs upon adenosine diphosphate (ADP) stimulation compared to washed platelets, whereas PEVs released upon stimulation with strong agonists (thrombin, collagen, A23187, U46619) were similar in the two groups. This different responsiveness to ADP was also observed as a higher α-granules release and protein kinase C activation in isolated platelets compared to washed ones. Residual plasma contamination appeared to be essential for the ability of platelets to release PEVs in response to ADP. Conclusions: In conclusion, our study strongly suggests that procedure adopted for platelets preparation is a critical determinant of PEVs release upon ADP stimulation.</p>
Beating Versus Arrested Heart Isolated Tricuspid Valve Surgery: Long-term Outcomes
<p>Russo M, Di Mauro M, Saitto G, Lio A, Berretta P, Taramasso M, Scrofani R, Della Corte A, Sponga S, Greco E, Saccocci M, Calafiore A, Bianchi G, Leviner DB, Biondi A, Della Ratta E, Livi U, Sharoni E, Werner P, De Vincentiis C, Di Eusanio M, Kocher A, Antona C, Miraldi F, Troise G, Solinas M, Maisano F, Laufer G, Musumeci F, Andreas M. Beating Versus Arrested Heart Isolated Tricuspid Valve Surgery: Long-term Outcomes. Ann Thorac Surg. 2022 Feb;113(2):585-592. doi: 10.1016/j.athoracsur.2021.03.070. Epub 2021 Apr 5. PMID: 33831392.</p> <p>Abstract</p> <p><strong>Background: </strong>Isolated tricuspid valve (TV) surgery is a rare procedure generally considered at high risk for perioperative mortality and poor long-term outcomes. Surgical treatment can be performed with either an arrested heart (AH) or beating heart (BH) technique. The aim of this study was to compare the outcomes of isolated tricuspid surgery with 2 different approaches.</p> <p><strong>Methods: </strong>The Surgical-Tricuspid Study is a multicenter international retrospective study enrolling adult patients who underwent isolated TV procedures (n = 406; age 56 ± 16 years; 56% female) at 13 international sites. The AH and BH strategies were performed in 253 and 153 patients, respectively. Propensity score-matched analysis was used to compare groups.</p> <p><strong>Results: </strong>After matching, 129 pairs were obtained and analyzed. The 30-day mortality rate was 6.2% versus 5.0% in the AH and BH groups, respectively (P = .9). The rates of acute renal failure requiring replacement therapy (10% versus 3%; P = .02) and stroke (1.6% versus 0%; P = .08) were numerically higher in the AH group. The 6-year survival rate was 67% ± 6% versus 78% ± 5% in the AH and BH groups, respectively (P = .18), whereas freedom from cardiac death was 75% ± 5% versus 84% ± 4% (P = .21). The 6-year composite cardiac end point of cardiac death and reoperation rate was 60% ± 9% versus 86% ± 5% (P = .024) comparing AH-TV replacement and BH-TV repair groups.</p> <p><strong>Conclusions: </strong>Isolated TV surgery performed with a BH strategy is a safe option and resulted in a trend of increased long-term survival and freedom from reoperation compared with the standard AH technique. Patients undergoing BH valve repair had the best long-term outcome.</p>
The rhizobia strain collection of Latvia University of Life Sciences and Technologies, containing strains from the historical rhizobia collection of Latvia University of Life Sciences and Technologies and newly isolated strains
<p>List of rhizobia and non-rhizobial endophyte bacterial strains, isolated from various legumes in Latvia. These strains are a part of the Rhizobia collection, Insitute of Soil and Plant Sciences, Latvia University of Life Sciences and Technologies. List include rhizobia strain identification, isolation year and host plant.</p>
REPARES interlaboratory study (ring test) on qPCR quantification of antibiotic resistance genes and other related genes in DNA isolated from sewage sludge and wastewater
<p>This dataset collects data on interlaboratory study (Ring test), performed within project REPARES in two phases (Ring test phase 1, Ring test phase 2). Sampling and analyses for phase 1 started on 25.2.2020 and for phase 2 on 27.9.2021. The sampling site was a wastewater treatment plant (> 100 000 inhabitants) in the Czech Republic.</p> <p>REPARES: Research platform on antibiotic resistance spread through wastewater treatment plants</p> <p>H2020-EU.4.b. ID 857552</p> <p>https://repares.vscht.cz/</p>
Transcription data for Pseudomonas aeruginosa isolates TBCF10839 and TBCF121838 grown to early and late exponential growth phase
GEO Series GSE36647. Pseudomonas aeruginosa. 8 samples. Type: Expression profiling by array.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.