Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
54,099
datasets available to search
ShareScore release 0.9.0
Dataset results
54,099 results for “Expression”
Full-length huntingtin Q19, Q23, Q42 and Q54 expression and purification – 2018/04/23
<p>Huntingtin structure-function open lab notebook project. Full-length huntingtin Q19, Q23, Q42 and Q54 expression and purification – 2018/04/23.</p>
Dataset for "Using Adaptive Immersive Environments to Stimulate Emotional Expression and Connection in Dementia Care: Insights from User Perspectives towards SENSE-GARDEN"
<p>This dataset contains all qualitative interview data recorded from early stage research on an adaptive, immersive, multi-sensory intervention that is being developed for people living with dementia (SENSE-GARDEN). 52 semi-structured interviews were conducted with people living with mild cognitive impairment, informal caregivers, and professional caregivers across Belgium, Norway, Portugal, and Romania. The aim of these interviews was to collect initial user responses towards SENSE-GARDEN. </p> <p>The pdf file "Registration Sheet and Interview Questions" lists the questions that were asked during the interviews. The excel file "Interview Data with Thematic Analysis" contains all raw interview data with ideas, notes, and codes made during thematic analysis. The first three sheets in the file correspond to the user type (Person with mild cognitive impairment/Informal Caregiver/Professional Caregiver). The fourth sheet, "Overall themes", gives an overview of each theme, subtheme, and relevant quotes belonging to these themes. </p> <p>This research was conducted as part of a larger project. The SENSE-GARDEN project (AAL/Call2016/054-b/2017, implementation period June 2017 - May 2020) is funded by AAL Programme, co-funded by the European Commission and National Funding Authorities of Norway, Belgium, Romania, and Portugal. </p>
Gene expression QTL lead variants in macrophages stimulated with IFNg and Salmonella
<p>Lead eQTL variants from RASQUAL and FastQTL analyses.</p> <p>Columns in the RASQUAL results:</p> <ul> <li>snp_id - id of the lead eQTL variant</li> <li>gene_id - id of the gene</li> <li>chisq - Chi-square statistics from RASQUAL</li> <li>p_nominal - Nominal p-value from RASQUAL</li> <li>p_eigen - Nominal p-value corrected by EigenMT for the number of indepedent tests in the cis region of the gene.</li> <li>n_tests - Number of independent tests estimated by EigenMT</li> <li>fdr_thresh - Empirical FDR 10% threshold. Those eQTLs for which p_eigen < fdr_threshold are significant at the 10% FDR level.</li> </ul> <p> </p>
Small scale test-expression of full-length huntingtin Q23 with HAP40 in baculoviral expression system production in sf9 insect cells – 2018/05/14
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Small scale test-expression of full-length huntingtin Q23 with HAP40 in baculoviral expression system production in sf9 insect cells – 2018/05/14. To validate our insect cell production system for the generation of physiologically relevant huntingtin constructs, HTT and HAP40 will be co-expressed and purified from sf9 culture. </p>
Fine vertical structures at the cloud heights of Venus revealed by radio holographic analysis of Venus Express and Akatsuki radio occultation data -- dataset
<p>The data used in the figures in the paper "Fine vertical structures at the cloud heights of Venus revealed by radio holographic analysis of Venus Express and Akatsuki radio occultation data" by Imamura et al. (J. Geophys. Res)</p> <p>The description of the columns in the files are given in the header section.</p>
Large scale expression and purification of full-length huntingtin Q23 with and without HAP40 from baculoviral expression system production in sf9 insect cells – 2018/06/04
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Purified huntingtin samples with and without stabilising binding partners (e.g. HAP40) are required for use in structural and functional studies, in particular SAXS experiments. </p>
Large scale expression and purification of full-length huntingtin Q23 with HAP40 from baculoviral expression system production in sf9 insect cells – 2018/05/28
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Purified huntingtin samples with stabilising binding partners (e.g. HAP40) are required for use in structural and functional studies, in particular SAXS experiments. </p>
Large scale expression and purification of full-length huntingtin Q19 and Q23 from baculoviral expression system production in sf9 insect cells – 2018/06/18
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Purified huntingtin samples are required for use in structural and functional studies. </p>
Large scale expression and purification of full-length huntingtin Q23 and Q54 from baculoviral expression system production in sf9 insect cells – 2018/07/06
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Purified huntingtin samples are required for use in structural and functional studies. </p>
Attempted expression and purification of full-length huntingtin Q23 with putative interaction partners from baculoviral expression system production in sf9 insect cells – 2018/07/19
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Rationale: </strong>To obtain monodisperse and conformationally constrained huntingtin protein samples for high resolution structural biology, interaction partners are required as highlighted by <a href="https://www.nature.com/articles/nature25502">Guo et al (2018)</a>.</p>
Large scale expression and purification of full-length huntingtin Q23 with HAP40 from baculoviral expression system production in sf9 insect cells – 2018/07/11
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Purified huntingtin samples are required for use in structural and functional studies. </p>
Exploring NSD3 Driven Gene Expression Profiles in Lung Squamous Cell Carcinoma
<p><strong>SGC Open Notebook Project to Characterize the HMTase NSD3</strong></p> <p><strong>Exp026 Objective:</strong> NSD3 is frequently amplified as part of the 8p11-12 focal amplification in several types of cancer. Here we set out to explore the gene expression profiles of NSD3 amplified versus non-amplified lung squamous cancer samples from the TCGA. However, because this event takes place in the context of a focal amplification we will also compare samples with high versus low NSD3 expression. The goal of this experiment is to identify putative oncogenic signalling pathways NSD3 may regulate, as well as inform on the biological role of this protein in the context of gene expression. </p>
Large scale expression and purification of full-length huntingtin Q42 from baculoviral expression system production in sf9 insect cells – 2018/07/10
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Purified huntingtin samples are required for use in structural and functional studies. </p>
Outgrowth of transferred tumors expressing libraries of PresentER minigenes in immunocompetent and immunodeficient mice
<p>Minigene sequencing of RMA/S tumors grown in RAG deficient mice, harvested and then transferred and grown in WT or RAG mice. "Pool 13" are wild type peptide minigenes. "Pool 18" are mutant peptide minigenes.</p>
Outgrowth of tumors expressing libraries of PresentER minigenes in vaccinated or unvaccinated immunocompetent mice
<p>Minigene sequencing of RMA/S tumors expressing libraries of PresentER peptide antigen minigenes after 17 days of growth in immunocompetent mice that were either vaccinated or nonvaccinated. "Pool 13" are wild type peptide minigenes. "Pool 18" are mutant peptide minigenes.</p>
Large scale expression and purification of huntingtin fragment A667-C3140 from baculoviral expression system production in sf9 insect cells – 2018/08/02
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Rationale: </strong>To obtain monodisperse and conformationally constrained huntingtin protein samples for high resolution structural biology.</p>
Finding an antibody to detect EZH1 protein expression – Part 2
<p>Follow up to previous post of trying to find an antibody to determine EZH1 expression in AML patient cells.</p>
Time-lapse images of yeast cells with different levels of TDH3 median expression and expression noise
<p>Time-lapse images of strains YPW3064, YPW3047, YPW2868 and YPW2879. Images were acquired with a Zyla sCMOS camera (Andor) on an Olympus IX83 inverted microscope equipped with a CoolLED pE-300 illumination system in Lab513 at University Paris Diderot. These images were used to produce Figure 5 - figure supplement 1 as well as the corresponding Source Data tables in the paper: Fitness effects of altering gene expression noise in Saccharomyces cerevisiae. Fabien Duveau, Andrea Hodgins-Davis, Brian P.H. Metzger, Bing Yang, Stephen Tryban, Elizabeth A. Walker, Patricia Lybrook, Patricia J Wittkopp (doi: https://doi.org/10.1101/294603).</p>
Finding an antibody to detect EZH1 protein expression – Part 3
<p>Follow up to previous post of trying to find an antibody to determine EZH1 expression in AML patient cells.</p>
Expression data of the flowering time genes in chickpea, extracted from Ridge et al. (2017). Plant Physiology 175, 802-815.
<p>This data is supplementary to the following paper: Gursky, V.V., Kozlov, K.N., Nuzhdin, S.V., and Samsonova, M.G. (2018) Dynamical Modeling of the Core Gene Network Controlling Flowering Suggests Cumulative Activation from the <em>FLOWERING LOCUS T </em>Gene Homologs in Chickpea. <em>Frontiers in Genetics</em>. 9:547. doi: 10.3389/fgene.2018.00547</p> <p>The data was obtained by digitizing Figure 5 of the following paper: Ridge, S., Deokar, A., Lee, R., Daba, K., Macknight, R. C., Weller, J. L., and Tar'an, B. (2017). The chickpea Early flowering 1 (Efl1) locus is an ortholog of arabidopsis ELF3. <em>Plant Physiology </em>175, 802-815. doi:10.1104/pp.17.00082</p> <p>The archive contains files (in csv format) with the expression data of each of the following ten genes: <em>FTa1</em>, <em>FTa2</em>, <em>FTa3</em>, <em>FTb</em>, <em>FTc</em>, <em>AP1</em>, <em>FD</em>, <em>TFL1a</em>, <em>TFL1c</em>, and <em>LFY</em>, for the cultivars CDC Frontier and ICCV 96029 and for two growth conditions (long day, LD, and short day, SD). Each file is named according to the following scheme: <Gene name>_<Cultivar name>_<Growth conditions>.csv. Each file contains values in the following three columns (separated by commas): time (in days after sowing), relative transcription level (%ACTIN), and standard error. In the case of the genes <em>AP1</em>, <em>FD</em>, <em>TFL1a</em>, <em>TFL1c</em>, and <em>LFY</em>, the standard error was assumed equal to the size of the points in the figure when the actual error range was smaller than that size (and, thus, not visible in the figure). In the case of the genes <em>FTa1</em>, <em>FTa2</em>, <em>FTa3</em>, <em>FTb</em>, and <em>FTc</em>, the standard error was recorded as 0 for such points (the error for these genes was not used in the study).</p> <p>The data was extracted with the help of the web-based tool <em>WebPlotDigitizer</em> (https://automeris.io/WebPlotDigitizer).</p>
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.