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101 results for “Phyllanthus”
Figure 4 from: Pornpongrungrueng P, Chantaranothai P, Parnell JA.N, Hodkinson TR (2019) Two new species of Phyllanthus (Phyllanthaceae) from Thailand. PhytoKeys 136: 35-44. https://doi.org/10.3897/phytokeys.136.47625
Figure 4 Phyllanthus chantaranothaii Pornp., J.Parn. & Hodk., sp. nov. A habit B branchlet showing axillary fascicle of staminate flowers C branchlet showing pistillate flower D branchlet showing young capsule E branchlet showing mature capsule. A–C photos by Natthawut Triyuttachai D, E photos by Siriyakorn Sukcharoen.
Figure 1 from: Pornpongrungrueng P, Chantaranothai P, Parnell JA.N, Hodkinson TR (2019) Two new species of Phyllanthus (Phyllanthaceae) from Thailand. PhytoKeys 136: 35-44. https://doi.org/10.3897/phytokeys.136.47625
Figure 1 Phyllanthus huamotensis Pornp., Chantar. & J.Parn., sp. nov. A habit B, C leaf shapes (B adaxial surface C abaxial surface) D stipule E pistillate flower F staminate flower G mature capsule. Drawn by Pimwadee Pornpongrungrueng.
Figure 3 from: Pornpongrungrueng P, Chantaranothai P, Parnell JA.N, Hodkinson TR (2019) Two new species of Phyllanthus (Phyllanthaceae) from Thailand. PhytoKeys 136: 35-44. https://doi.org/10.3897/phytokeys.136.47625
Figure 3 Phyllanthus chantaranothaii Pornp., J.Parn. & Hodk., sp. nov. A habit B, C leaf shapes (B adaxial surface C abaxial surface) D stipule E pistillate flower F staminate flower G young capsule H mature capsule. Drawn by Pimwadee Pornpongrungrueng.
Figure 2 from: Pornpongrungrueng P, Chantaranothai P, Parnell JA.N, Hodkinson TR (2019) Two new species of Phyllanthus (Phyllanthaceae) from Thailand. PhytoKeys 136: 35-44. https://doi.org/10.3897/phytokeys.136.47625
Figure 2 Phyllanthus huamotensis Pornp., Chantar. & J.Parn., sp. nov. A, B habit C branchlet showing axillary fascicle of staminate flowers D branchlet showing pistillate flower E branchlet showing young red capsule. A Photo by Natthawut Triyuttachai B, C photos by Suchart Chanhomhual D, E photos by Kanokorn Ruengsawang.
Supplementary material 1 from: Sadaqa E, Utami RA, Mudhakir D (2024) In vitro cytotoxic and genotoxic effects of Phyllanthus niruri extract loaded chitosan nanoparticles in TM4 cells and their influence on spermatogenesis. Pharmacia 71: 1-14. https://doi.org/10.3897/pharmacia.71.e112138
Supplementary information
Figure 7 from: Sadaqa E, Utami RA, Mudhakir D (2024) In vitro cytotoxic and genotoxic effects of Phyllanthus niruri extract loaded chitosan nanoparticles in TM4 cells and their influence on spermatogenesis. Pharmacia 71: 1-14. https://doi.org/10.3897/pharmacia.71.e112138
Figure 7 Imaging of cellular morphological change in the presence of ChNP and PNNP. TM4 cells were incubated in the absence of nanoparticles (a) and in the presence of ChNP at 50 µg/mL (b), PNNP at 125 µg/mL (c), and ChNP at 200 µg/mL (d). Images were captured 2 h after transfection using a 20× lens on a CLSM (Olympus FV-1200) and the nucleus was stained with Hoechst 33342 (blue). The bar indicates 50 µm.
Figure 9 from: Sadaqa E, Utami RA, Mudhakir D (2024) In vitro cytotoxic and genotoxic effects of Phyllanthus niruri extract loaded chitosan nanoparticles in TM4 cells and their influence on spermatogenesis. Pharmacia 71: 1-14. https://doi.org/10.3897/pharmacia.71.e112138
Figure 9 Claudin 11 expression in the presence of ChNP and PNNP. TM4 cells were incubated with ChNP 50 µg/mL, PNNP 125 µg/mL and ChNP 200 µg/mL for 24 h. a. Confocal images of claudin 11 expression after cells were incubated with a secondary antibody goat anti-rabbit IgG Alexa Fluor 488 (green) and the nucleus was stained with Hoechst 33342 (blue). The bars indicate 50 µm; b. Histogram of downregulation claudin 11 expression. The results are shown as mean ± SE of two separate studies (n = 50). ns (not significant) p > 0.05. Stars (***) indicate significance when compared with the control group (untreated cells) (p < 0.001). Pound signs (###) demonstrate significance compared with ChNP 50 µg/mL.
Figure 8 from: Sadaqa E, Utami RA, Mudhakir D (2024) In vitro cytotoxic and genotoxic effects of Phyllanthus niruri extract loaded chitosan nanoparticles in TM4 cells and their influence on spermatogenesis. Pharmacia 71: 1-14. https://doi.org/10.3897/pharmacia.71.e112138
Figure 8 Connexin 43 expression in the presence of ChNP and PNNP. TM4 cells were incubated with ChNP 50 µg/mL, PNNP 125 µg/mL and with ChNP 200 µg/mL for 24 h. a. Confocal images of connexin 43 expression after cells were incubated with a secondary antibody goat anti-rabbit IgG Alexa Fluor 488 (green) and the nucleus was stained with Hoechst 33342 (blue). The bars indicate 50 µm. b. Histogram of downregulation of connexin 43 expression. The results are shown as mean ± SEM of two separate studies (n=50). ns (not significant) p > 0.05. Stars (***) indicate significance when compared with the control group (untreated cells) (p < 0.001). Pound signs (###) demonstrate significance compared with ChNP 50 µg/mL.
Figure 6 from: Sadaqa E, Utami RA, Mudhakir D (2024) In vitro cytotoxic and genotoxic effects of Phyllanthus niruri extract loaded chitosan nanoparticles in TM4 cells and their influence on spermatogenesis. Pharmacia 71: 1-14. https://doi.org/10.3897/pharmacia.71.e112138
Figure 6 DNA breakage caused by ChNP and PNNP using FHA. TM4 cells were incubated with various concentration of ChNP (25, 50, 200 µg/mL) and PNNP (62.5, 125, 500 µg/mL) for 2 h. Positive control 100 µm H2O2 was used and incubated for 15 min. Photomicrograph obtained by Inverted Microscope Olympus IX73. Single stranded DNA was stained with ethidium bromide of a. NEgative control (untreated cells); b. H2O2; c.ChNP 25 µg/mL; d.ChNP 50 µg/mL; e.ChNP 200 µg/mL; f.PNNP 62.5 µg/mL; g.PNNP 125 µg/mL; h.PNNP 500 µg/mL. The bar indicates 5 µm. i. Histogram showing DNA damage level using NDF from several randomly selected microscope images. The results are shown as mean ± SEM of three independent experiments (n=150). ns (not significant) p > 0.05. ***p < 0.001 when compared to the corresponding control group.
Figure 4 from: Sadaqa E, Utami RA, Mudhakir D (2024) In vitro cytotoxic and genotoxic effects of Phyllanthus niruri extract loaded chitosan nanoparticles in TM4 cells and their influence on spermatogenesis. Pharmacia 71: 1-14. https://doi.org/10.3897/pharmacia.71.e112138
Figure 4 Cell viability percentage after 24 hours of exposure to various concentrations of ChNP and PNNP assessed by CCK8 test. The values are presented as mean ± standard deviation (SD) (n = 3). All data were analyzed using One-way ANOVA. The stars indicate significant difference between control negative (untreated cells) and various treatment groups (P < 0.05 significantly different, p-value ≥ 0.05 was determined as non-significant (ns). A p-value score of between 0.01 and 0.05 was considered significant (*), between 0.01 and 0.001 as very significant (**), and < 0.001 as extremely significant (***).
Figure 3 from: Sadaqa E, Utami RA, Mudhakir D (2024) In vitro cytotoxic and genotoxic effects of Phyllanthus niruri extract loaded chitosan nanoparticles in TM4 cells and their influence on spermatogenesis. Pharmacia 71: 1-14. https://doi.org/10.3897/pharmacia.71.e112138
Figure 3 Illustration of the TGA, DTG, and DTA curves for A.ChNP and B.PNNP. DTA curves are represented in green, DTG curves in red, and TGA curves in blue.
Figure 5 from: Sadaqa E, Utami RA, Mudhakir D (2024) In vitro cytotoxic and genotoxic effects of Phyllanthus niruri extract loaded chitosan nanoparticles in TM4 cells and their influence on spermatogenesis. Pharmacia 71: 1-14. https://doi.org/10.3897/pharmacia.71.e112138
Figure 5 DNA damage caused by ChNP and PNNP using comet assay. TM4 cells were incubated with various concentrations of ChNP and PNNP for 2 h. Positive control 100 µm H2O2 was used and incubated for 15 min. Comet images demonstrating the degree of DNA damage on TM4 cells were captured using Inverted Microscope Olympus IX73. Single stranded DNA was stained with ethidium bromide. Comet images displayed untreated cells as control (a), H2O2-treated cells (b), and ChNP-treated cells at concentrations of 25 µg/mL (c), 50 µg/mL (d), and 200 µg/mL (e), as well as PNNP-treated cells at concentrations of 62.5 µg/mL (f), 125 µg/mL (g), and 500 µg/mL (h). The bar indicates 5 µm. Quantitative assessment of DNA damage was performed by measuring olive tail moment (i) and % tail DNA (j). The data are presented as the mean ± SEM of three independent trials (n=200), ns (not significant) (***p < 0.001, **p < 0.01, *p < 0.05 when compared to the corresponding control group).
Figure 1 from: Sadaqa E, Utami RA, Mudhakir D (2024) In vitro cytotoxic and genotoxic effects of Phyllanthus niruri extract loaded chitosan nanoparticles in TM4 cells and their influence on spermatogenesis. Pharmacia 71: 1-14. https://doi.org/10.3897/pharmacia.71.e112138
Figure 1 Transmission electron microscope (TEM) images showing morphology of A. unloaded ChNP compared to B.PNNP. The scale bar represents 100 nm.
FIGURE 3. Phyllanthus pterocaulis M.J in A new Critically Endangered species of stone breaker (Phyllanthus, Phyllanthaceae) from Central Brazil, with notes on its leaf and stem anatomy
FIGURE 3. Phyllanthus pterocaulis M.J. Silva. Distribution map.
Figure 1 from: Anto EJ, Syahputra RA, Silitonga HA, Situmorang PC, Nugaraha SE (2022) Oral acute toxicity study extract ethanol of balakka fruit (Phyllanthus emblica). Pharmacia 69(1): 187-194. https://doi.org/10.3897/pharmacia.69.e81280
Figure 1 Histopathology of Organs including the heart, testis, ovary, liver, and kidney from CM, CF, M2000 (M2), F2000 (F2), M5000 (M5), and F5000 (F5) (H&e x20). Testis ST: seminiferous tubules; IT: Intestinal tissue. Heart MC: cardiac muscle cells; MC: muscle nucleus. Ovary O: Oocytes. Liver tissue PA: portal area; H: hepatocytes. Kidney tissue G: glomerulus; BS: Bowmen space, DT: Distal Concoluted tubule.
Phyllanthus urinaria L. (BR0000015221209V)
Belgium Herbarium image of <a href="https://www.plantentuinmeise.be">Meise Botanic Garden</a>.
Phyllanthus fraternus G.L.Webster (BR0000012562459)
Belgium Herbarium image of <a href="https://www.plantentuinmeise.be">Meise Botanic Garden</a>.
Fig. 2. Phyllanthus novofriburgensis J.C.R.Mendes, J.M.A in Phyllanthus novofriburgensis and P. pedrosae, two new species of Phyllanthus subsect. Clausseniani (Phyllanthaceae) from Southeastern Brazil
Fig. 2. Phyllanthus novofriburgensis J.C.R.Mendes, J.M.A.Braga & Fraga sp. nov. A‒B. Habitat. C‒D. Branches (in detail the staminate and pistillate flowers). E. Capsule. Photos by J.C.R. Mendes (A, B, C, E) and R. Freitas (D with staminate flower detail).
FIGURE 4 in Phyllanthus eremitus (Phyllanthaceae), a narrowly endemic new species from Santa Catarina, southern Brazil, and lectotypification and range extension of P. hyssopifolioides
FIGURE 4. Habitat of Phyllanthus eremitus.
FIGURE 3 in Typifications and a new synonym in Phyllanthus sect. Phyllanthus subsect. Clausseniani (Phyllanthaceae)
FIGURE 3. Updated geographical distribution of Phyllanthus heteradenius.
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