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18,621 results for “development;”
Dataset of questionnaires for teachers developed in the frame of ENVRIPLUS project
<p>The questionnaire is addressed to teachers in order to catch their needs and help in feeding the platform with targeted contents. The first part of the questionnaire focuses on objective information about the formal, quantitative and qualitative position of science class in schools, and the content and methods of teaching in different countries. The second part of the questionnaire investigates personal teacher experiences and their views on what can improve training offer about environmental science lessons and courses. The third part investigates on tools teachers have and use and on what they would have. Questionnaires were collected based on the GITF international network and in the occasion of training events for Italian teachers.This action is undertaken for the developing of Task 15.5, aimed at providing secondary school teachers and students with an e-Training Platform, based on scientific themes related to Research Infrastructures for the Environmental Sector participating to ENVRIplus project. The main actions are aimed to design, structure, develop and execute a Training course for teachers to the use of the multimedia platform.</p>
Dataset: SiNGA Simulation of cAMP sink development in different environments.
<p>The setup files for the simulation of cAMP sink development in different environments. The results of the simulations were presented on the ISMB Sysmod Meeting 2019.</p> <p>To run a simulation the desired simulation setup file must be located in the same directory as the "singa-run.jar" and must be named "simulation_setup.json". Afterwards the simulation can be run using the "java -jar singa-run.jar" command on any system with Java 8 installed. Simulation parameters may be altered by changing the simulation setup file. The "singa-run" jar in this depository automatically runs all simulations resulting from all combinations of the "alternative values" annotated to concentrations or features.</p> <p>Results are saved in an "observations" folder that is created in the same directory. Each simulation creates one folder containing the variations used and the trajectory of the simulation.</p> <p>More information are available on the <a href="https://github.com/singa-bio/singa">SiNGA github</a>.</p>
Test data set for macros accompanying the publication Multi-parameter screening method for developing optimized red fluorescent proteins
<p>This a bundle of test data can be used to run the macros accompanying the publication Multi-parameter screening method for developing optimized red fluorescent proteins.</p> <p>These data sets can be used to run the following macros that can be found on GitHub:</p> <ol> <li><a href="https://github.com/molcyto/MC-Ratio-96-wells">https://github.com/molcyto/MC-Ratio-96-wells</a></li> <li><a href="https://github.com/molcyto/MC-Ratio-Petri-dish">https://github.com/molcyto/MC-Ratio-Petri-dish</a></li> <li><a href="https://github.com/molcyto/MC-FLIM-Petri-dish">https://github.com/molcyto/MC-FLIM-Petri-dish</a></li> <li><a href="https://github.com/molcyto/MC-Bleach-96-wells">https://github.com/molcyto/MC-Bleach-96-wells</a></li> <li><a href="https://github.com/molcyto/MC-Scatter5D">https://github.com/molcyto/MC-Scatter5D</a></li> <li><a href="https://github.com/molcyto/MC-FLIM-96-wells">https://github.com/molcyto/MC-FLIM-96-wells</a></li> </ol> <p>Funding:<br> This work was supported by the NWO CW-Echo grant 711.011.018 (M.A.H. and T.W.J.G.), grant 12149 (T.W.J.G.) from the Foundation for Technological Sciences (STW) from the Netherlands</p> <p> </p>
PERCEIVE Education, Research Development and Innovation in the South-East Development Region A Case Study 20191006
<p>The data set include secondary data extracted from EUROSTAT database. The data set referring to the education and research infrastructure on Sud-Est NUTS II region from Romania for the period 2007-2013. </p>
Splicing patterns of the Arabidopsis organellar rhomboid At1g74140 change during development from young to mature leaf tissues.
<p><strong>Background to the study:</strong> Four distinct rhomboid genes appear to function in <em>Arabidopsis</em> plastids, two “active” types from the secretases and presenilin-like associated rhomboid-like (PARL) categories (At1g25290 and At5g25752) and two “inactive” rhomboid forms (At1g74130 and At1g74140). The number of working rhomboids is further increased by alternative splicing, two reported for At1g25290 and three for At1g74130. Since At1g25290 and At1g74130 exist as alternative splice variants, it would be necessary to assess the splicing patterns of the other two plastid rhomboid genes, At5g25752 and At1g74140, before studying the <em>Arabidopsis</em> plastid rhomboid system as a whole. </p> <p><strong>Method used:</strong> This study thus specifically focused on an analysis of the At1g74140 transcript population using various RT-PCR strategies. The supplementary table provides the details of the various RT-PCR primers used in this study.</p> <p><strong>Results of the study and their relationship to the supplementary figures deposited here:</strong> The exon mapping results indicate splicing patterns different from the close relative At1g74130, despite similarity between the exonic sequences. The splicing patterns indicate a high level of sequence “discontinuity” in the At1g74140 transcript population with a significant portion of the discontinuity being generated by two regions of the gene. The three supplementary figures of RT-PCR results deposited here provide further evidence that the alternatively spliced short transcript products from the discontinuous splicing patterns fluctuate during development.</p> <p><strong>Conclusion of the main study and its relationship to the supplementary results deposited here:</strong> The overall discontinuous splicing pattern of At1g74140 may be reflective of its mode of involvement in activities like controlling gene expression. The developmental evidence in these deposited supplementary RT-PCR results indicate that some of the alternatively spliced short transcript products fluctuate between mature and young tissues, suggesting that the short transcripts bear functional significance.</p>
Figures 1-6 in Gonadal development and fecundity of the smooth weakfish Cynoscion leiarchus (Teleostei: Perciformes: Sciaenidae) in a tropical Brazilian bay
Figures 1-6. Photomicrographs of ovarian (1-4) and testicular (5-6) histology illustrating oocytes and spermatocytes at different development stages of C. leiarchus: (1-2) regenerating ovarian phase; (3) spawning capable ovarian phase; (4) actively spawning subphase; (5- 6) spawning capable testis phase. (PG) Primary growth, (MB) muscle bundle, (CA) cortical alveolar, (OW) ovarian wall, (Vtg1) primary vitellogenic, (Vtg2) secondary vitellogenic, (Vtg3) tertiary vitellogenic, (A) atresia POF: postovulatory follicle complex, (GVM) germinal vesicula migration, (GVBD) germinal vesicular breackdown, (Sg1) primary spermatogonia, (Sz) spermatozoa, (Cy) spermatocyst.
8 9 10 11 Figures 8-11 in Gonadal development and fecundity of the smooth weakfish Cynoscion leiarchus (Teleostei: Perciformes: Sciaenidae) in a tropical Brazilian bay
8 9 10 11 Figures 8-11. Relationship between batch fecundity and gonad weight (8), total weight (9), total length (10), and age (11) of Cynoscion leiarchus in Sepetiba Bay.
Fig. 8. Mastigograptus aff. tenuiramosus. A in The ultrastructure, development, and systematic position of the graptolite genus Mastigograptus
Fig. 8. Mastigograptus aff. tenuiramosus. A. Thecal base of Fig. 6B. Scale bar 50 µm. B, C. Fractured thecal bases, and occluded autotheca (arrowed) (ZPAL G.30/17). Scale bars: B, 100 µm, C, 50 µm. D. Thecal base on main stem, with occluded autotheca (arrowed), a needle preparation from ZPAL G.30/18. Scale bar 20 µm. E. Stem, looking obliquely proximally, with two thecal bases (ZPALG.30/15). Scale bar 200 µm. F, G, H. Successively more distal cross−sections of thecal bases. Scale bars 10 µm, 50 µm, 100 µm. I. Thecal base with occluded autotheca (ZPALG.30/17). Scale bar 50 µm. J. Diaphragm of stolonal triad in thecal base, looking proximally (ZPALG.30/15). Scale bar 10 µm. K. Stolotheca looking proximally with adjacent thecal base displaying astolothecaltriad,seeH(ZPALG.30/11).Scalebar200µm. L.Crassal,fusellarandcorticaltissuesinthecalbase(ZPALG.30/17).Scalebar5µm. M."E.T. head" of thecal base, with central occluded autotheca (ZPAL G.30/4). Scale bar 50 µm.
Fig. 4. Mastigograptus aff. tenuiramosus. A in The ultrastructure, development, and systematic position of the graptolite genus Mastigograptus
Fig. 4. Mastigograptus aff. tenuiramosus. A. Fractured wall of stolotheca. The head of a fusellus is arrowed (ZPALG.30/9). Scale bar 10 µm B, C. External ornament on stolothecae (ZPAL G.30/10). Scale bars 100 µm, 50 µm. D. Sectional view of stolonal triad on main stem, looking distally (ZPAL G.30/11). Scale bar 50 µm. E. Stolonal triad on main stem, looking proximally; diaphragm at base of occluded autotheca (arrowed) (ZPALG.30/12). Scale bar 50 µm. F. Base of diaphragm on main stem, looking distally, and showing two pores (ZPALG.30/13). Scale bar 10 µm. G. Portion of stem with monopodial branch to right. a, occluded first thecal base on branch, with short stolotheca. b, borings on main stem (ZPAL G.30/14). Scale bar 500 µm.
Fig.3. Mastigograptus aff. tenuiramosus. A in The ultrastructure, development, and systematic position of the graptolite genus Mastigograptus
Fig.3. Mastigograptus aff. tenuiramosus. A.Stipewiththickcortexandfracturedthecalbase.(ZPALG.30/4).Scalebar100µm. B.Occludedthecalbaseon proximal portion of stem (ZPALG.30/3). Scale bar 10 µm. C. Proximal portion of stem, with occluded sicula and first thecal base. (ZPALG.30/2). Scale bar 200 µm. D. Portion of stem with monopodial branch (ZPALG.30/5). Scale bar 2 µm. E. Fractured stolotheca; distal to left (ZPALG.30/6). Scale bar 50 µm. F. Fractured stem with thick cortex and borings (ZPALG.30/7). Scale bar 50 µm. G. Amphorate stolothecae, and broken thecal bases (ZPALG.30/4) Scale bar 1 mm. H. Amphorate stolotheca, looking proximally. (ZPAL G.30/8). Scale bar 100 µm. I. Stereopair view of thecal base (ZPAL G.30/4). Scale bar 100 µm.
Fig. 2. Mastigograptus aff. tenuiramosus. A in The ultrastructure, development, and systematic position of the graptolite genus Mastigograptus
Fig. 2. Mastigograptus aff. tenuiramosus. A. Fusellar fabric of holdfast (ZPAL G.30/2). Scale bar 1 µm. B. Fusellar and cortical fabrics in the laminae of holdfast (ZPAL G.30/3). Scale bar 20 µm. C. Sheet fabric of laminae of holdfast (ZPAL G.30/1). Scale bar 1 µm. D. Laminae of holdfast. Bundled parallel cortical fibrils and sheet fabric with vesicles (ZPALG.30/1). Scale bar 1 µm. E. Two siculae on holdfast. The left hand one is occluded (ZPALG.30/1). Scale bar 100 µm. F. Occluded sicula (arrowed) (ZPAL G.30/3). Scale bar 100 µm. G, H. Broken thecal base on proximal portion of stem (ZPAL G.30/2). Scale bars: G, 50 µm; H, 100 µm.
Fig. 3 in Development and morphological characterization of the immature stages of Tetrastichus giffardianus Silvestri (Hymenoptera: Eulophidae)
Fig. 3. Immature stages of Tetrastichus giffardianus. Egg (A); first-instar larva (B, C); second-instar larva (D); third-instar larva (E); pre-pupa (F); pupa at three different stages of melanization (G, H, I). Scale bar = 0.1 mm.
Fig. 1 in Development and morphological characterization of the immature stages of Tetrastichus giffardianus Silvestri (Hymenoptera: Eulophidae)
Fig. 1. Tetrastichus giffardianus larvae (A) and pupae (B) inside a Ceratitis capitata puparium; (C) emergence of adults; (D) C. capitata puparium with T. giffardianus emergence hole.
Fig. 3 in Intra-puparial development of the Cochliomyia macellaria and Lucilia cuprina (Diptera, Calliphoridae)
Fig. 3. Morphological sequence of the intra-puparial development of Lucilia cuprina. (A) ventral view of the cryptocephalic pupa; (B, C) ventral view of the phanerocephalic pupa; (D) ventral view of the pharate adult and (E) dorsal view, anterior spiracle of the pupa (arrow); (F) ventral view of the pharate adult, sequence of pigmentation of the hairs, bristles and body structures; (G) formed adult and formation of the ptilineal sac in the dorsal view, anterior spiracle of the pupa (arrow) and (H) ventral view. Scale: 1 mm.
Fig. 1 in Intra-puparial development of the Cochliomyia macellaria and Lucilia cuprina (Diptera, Calliphoridae)
Fig. 1. Morphological sequence of the intra-puparial development of Cochliomyia macellaria. (A) Ventral view and (B) dorsal view, cryptocephalic pupa; (C) ventral view, phanerocephalic pupa; (D, E) dorsal and (F) ventral view, pharate adult; (G) Pharate adult in dorsal view, sequence of bristle pigmentation and body sutures; (H) Imago with formation of the ptilineal sac in ventral view. Arrow, anterior spiracle (asp). Scale: 1 mm.
Figure 1 in Development of experimental mesocosms for cicada nymphs Graptopsaltria nigrofuscata: methodology and research recommendations
Figure 1. Photographs of the mesocosm experiment. (A) a final instar nymph of Graptopsaltria nigrofuscata cicada in a mesocosm cage. (B) An empty burrow made by a cicada nymph. The nymph might feed on larch root at the interior of burrow. Photographs were taken at the end of mesocosm experiment (7 July).
Fig. 8 in Ultrastructure of the Plasmodial Development of Myxobolus insignis (Myxozoa), Infecting the Amazonian Fish Semaprochilodus insignis (Prochilodontidae)
Fig. 8. Schematic drawing of the plasmodial evolution of the periphery of plasmodia (P) of Myxobolus insignis n. sp. showing the differentiation of the plasmalemma during the three stages (a – Stage 1; b – Stage 2 and c – Stage 3) during sporogenesis (Fb – fibroblasts, P – plasmodia, Ve – vesicles, * – collagen fibres).
Figs 1–4 in Ultrastructure of the Plasmodial Development of Myxobolus insignis (Myxozoa), Infecting the Amazonian Fish Semaprochilodus insignis (Prochilodontidae)
Figs 1–4. Light and ultrastructural aspects of the periphery of plasmodia of Myxobolus insignis infecting the freshwater teleost Semaprochilodus insignis from the Amazon River, showing the evolution of the plasmalemma during the three phases. 1 – light micrograph showing the smooth plasmalemma (arrowheads) of the plasmodium (P) in contact with the surrounding cells of the host (H). This aspect corresponds to stage 1 of the plasmodium development; 2 – ultrastructural aspect of the area boxed in Fig. 1, observed with higher magnification showing several collagen fibres (Cg) at the periphery of the plasmodium (P) and some vesicles (Ve) in the cortical zone of the plasmodium; 3 – light micrograph showing the plasmalemma containing several small microvilli (arrowheads). Internally, some immature (iS) and mature spores (mS) and vesicles are present and, externally, a layer of collagen fibres (Cg). This aspect corresponds to stage 2 of the plasmodium development; 4 – ultrastructural aspect of the plasmalemma and their microvilli (arrowheads) in contact with surrounding fibroblasts (Fb). Among the microvilli numerous collagen fibres are present. The cortical zone of the plasmodium (P) contains numerous vesicles (Ve), some of which seem to fuse with the plasmalemma.
Figs 5–7 in Ultrastructure of the Plasmodial Development of Myxobolus insignis (Myxozoa), Infecting the Amazonian Fish Semaprochilodus insignis (Prochilodontidae)
Figs 5–7. Ultrastructural aspects of the periphery of the plasmodia (P) of Myxobolus insignis n. sp. infecting the freshwater teleost Semaprochilodus insignis from the Amazon River showing the different aspects of the microvilli (arrowheads) and collagen fibre arrangements (Cg) located among the fibroblasts (Fb), some of which are showing their nuclei (Nu). The cortical zone of the plasmodium contains numerous vesicles (Ve).
Figure 4 in Development of empirical standard weight equation for Pursak chub Squalius pursakensis, an endemic cyprinid species of Northwest Anatolia
Figure 4. Plots showing the distribution of the residuals (a) and the results of the application of the empirical quartiles (EmpQ) method (b) used to investigate potential length bias in the standard mass (W s) equation for Squalius pursakensis. Residuals = standardized residuals of the regression; fitted values = values obtained by the model fit; standardized 75th percentile mean W = standardized 75th percentile mean weights calculated by W equation.
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.