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opennotspecifiedAug 2021View details →

Fig. 3 in Pyrazinopyrimidine alkaloids from a mangrove-derived fungus Aspergillus versicolor HDN11-84

Fig. 3. DP4+ probability analyses for candidates diastereomers of 1 and 2 (Gibbs free energies at the PCM/B3LYP/6-31+G (d,p) level were used for the analysis).

Fig. 6 in Genome-wide transcriptional analysis unveils the molecular basis of organ-specific expression of isosteroidal alkaloids biosynthesis in critically endangered Fritillaria roylei Hook

Fig. 6. Heatmap representing organ specific significantly enriched genes corresponding to (A) Isosteroidal alkaloid biosynthesis, (B) Sucrose and starch metabolism, (C) UGTs and CYPs, (D) aquaporins, (E) ABC transporters, (F) Transcription factor and Transposable elements. The red-blue scale represents positive enrichment (red) and negative enrichment (Blue) of transcripts. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 5 in Genome-wide transcriptional analysis unveils the molecular basis of organ-specific expression of isosteroidal alkaloids biosynthesis in critically endangered Fritillaria roylei Hook

Fig. 5. Significantly enriched transcripts (nodes) in predicted interactome network. (A) Steroidal alkaloid biosynthesis pathways (B) Isopentenyl diphosphate biosynthesis pathway, (C) Sucrose and starch metabolic pathways and (D) Aquaporins. The nodes encircled in red color represents higher enrichment in the bulb while the nodes encircled in grey and blue represents higher enrichment in arial organs (leaf and stem). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 7 in Genome-wide transcriptional analysis unveils the molecular basis of organ-specific expression of isosteroidal alkaloids biosynthesis in critically endangered Fritillaria roylei Hook

Fig. 7. qRT-PCR expression-based validation of RNA-seq data using potential 20 genes involved in isosteroidal alkaloid biosynthetic pathway. (A) Bulb vs. Leaf, (B) Leaf vs. Stem and (C) Bulb vs. Stem.

Fig. 3 in Genome-wide transcriptional analysis unveils the molecular basis of organ-specific expression of isosteroidal alkaloids biosynthesis in critically endangered Fritillaria roylei Hook

Fig. 3. Significant KEGG pathway enrichment analysis in tissues from aerial organs (leaf & Stem) and bulb. The green colour enrichment indicates higher expression in aerial tissue while pink represents higher enrichment in bulb. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 4 in Genome-wide transcriptional analysis unveils the molecular basis of organ-specific expression of isosteroidal alkaloids biosynthesis in critically endangered Fritillaria roylei Hook

Fig. 4. Transcriptional protein-protein interactome (PPIN) prediction in F. roylei along the network statistics. (A) Overall prediction of PPI network based on significantly differential expressed transcripts. Spatial PPI network prediction of significantly enriched transcripts in (B) Bulb, (C) Leaf and (D) Stem.

Fig. 1 in Genome-wide transcriptional analysis unveils the molecular basis of organ-specific expression of isosteroidal alkaloids biosynthesis in critically endangered Fritillaria roylei Hook

Fig. 1. Summary of organ specific spatial transcriptome sequencing in F. roylei. (A): Overall quality filtering of sequenced reads; (B): Tissue specific high quality filtered reads obtained after removing low quality/adaptor contaminated sequences; (C): Assembly statistics details; (D): Venn diagram representing functional annotation with six different public protein databases.

Fig. 8 in Genome-wide transcriptional analysis unveils the molecular basis of organ-specific expression of isosteroidal alkaloids biosynthesis in critically endangered Fritillaria roylei Hook

Fig. 8. Representation of isosteroidal alkaloid biosynthesis pathway in F. roylei and heat map representing expression of genes in Stem, Leaf and Bulb tissue using red-blue scale (red: positive enrichment and Blue: negative enrichment of transcripts). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 2 in Genome-wide transcriptional analysis unveils the molecular basis of organ-specific expression of isosteroidal alkaloids biosynthesis in critically endangered Fritillaria roylei Hook

Fig. 2. Clustering of 2488 significant differentially expressed transcripts in leaf, stem and bulb in F. roylei (A) sub-cluster 1 represent transcripts with significant higher expression in bulb; (B): Sub-cluster 2 in Stem and (C): Sub-cluster 3 in leaf; (D): Pearson's correlation of organ specific significant differentially expressed clustered transcripts in leaf, stem and bulb tissues.

Fig. 4 in The complexity of sound quantification of specialized metabolite biosynthesis: The stress related impact on the alkaloid content of Catharanthus roseus

Fig. 4. The stress-related increase of the concentration of natural products. In principle, two major effects are responsible for the stress-related increase, i. e., the decrease of the reference value (e.g. dry weight), and an enhancement of biosynthetic activity. The latter one is due either to stress-related up-regulation ("active increase" of enzymatic activity) or a "passive shift" cause by the stress-related overreduction due to stomatal closure. Decreases of factors are displayed in red, the related enhancements in blue. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 3 in The complexity of sound quantification of specialized metabolite biosynthesis: The stress related impact on the alkaloid content of Catharanthus roseus

Fig. 3. Dry weight, alkaloid concentration, and alkaloid content in leaves of Catharanthus roseus plants under salt stress. (a): Dry weight of the entire leaves; (b): Concentration of alkaloids in old leaves; (c): Concentration of alkaloids in young leaves; (d): Total alkaloids content in all aerial plant parts. Differentiation between young and old leaves is mentioned in the Materials and methods section. Different lower-case letters on top of the columns for each period of time (10 and 20 days) indicate significant differences (P ≤ 0.05) as calculated using the least significant difference (LSD) test; n = 8. Every period has 5 bars, and these bars from left to right are control, 100 mM, 200 mM, 300 mM, 400 mM NaCl solution, respectively. The bars display the standard deviation.

Fig. 1 in The complexity of sound quantification of specialized metabolite biosynthesis: The stress related impact on the alkaloid content of Catharanthus roseus

Fig. 1. Evapotranspiration rate of drought-stressed Catharanthus roseus plants. The evapotranspiration rates for mild (20% watering) and severe drought stress (40% watering) were obtained by calculating the amount of water lost. Control plant results were used as a reference to normalize the rate. Arrows indicate the days of sampling.

Fig. 5 in Pyrrolyl 4-quinolone alkaloids from the mangrove endophytic fungus Penicillium steckii SCSIO 41025: Chiral resolution, configurational assignment, and enzyme inhibitory activities

Fig. 5. Molecular docking result of (+)-1 in α-glucosidase (PDB ID: 5NN8). (A) 3D structure of the enzyme docked with (+)-1. (B) Detail analysis of the 2D binding model of (+)-1 with the residues surrounding the binding pocket of α-glucosidase.

Fig. 4 in Indole alkaloids from the leaves of Ravenia spectabilis engl. with activity against pancreatic cancer cell line

Fig. 4. Interaction of compounds 1 (A), 2 (B) and 3 (C) with DNA sequence Seq-1. All three compounds are predicted to intercalate with the DNA base pairs.

Fig. 3 in Indole alkaloids from the leaves of Ravenia spectabilis engl. with activity against pancreatic cancer cell line

Fig. 3. Morphological changes observed in HeLa (top row) and MIA PaCa-2 (bottom row) cells after incubation with gemcitabine (A and E), compound 1 (B and F), compound 2 (C and G) and compound 3 (D and H) at their respective IC50s for 8 h.

Fig. 4 in Alkaloids bearing rare skeletons from Forsythia suspensa with anti-inflammatory and anti-viral activities in vitro

Fig. 4. Experimental CD spectrum of 1 (in red) in MeOH and calculated ECD spectra of 1a (4bS,8S,8aR, in black) and 1b (4bR,8R,8aS, in blue) at the b3lyp/6–31 +g (d,p) level in MeOH. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

opennotspecifiedOct 2021View details →

Fig. 4 in Alkaloids from Lepidium meyenii (Maca), structural revision of macaridine and UPLC-MS/MS feature-based molecular networking

Fig. 4. Cluster containing identified imidazole and amidine alkaloids. Nodes were numbered from lowest to highest m/z values. Detailed information on unknown nodes can be found as Supporting Information.

opennotspecifiedDec 2021View details →

Fig. 4 in Diterpenoid alkaloids from Delphinium forrestii var. viride and their anti-inflammation activity

Fig. 4. Effect of compounds 1–15 on LPS-induced production of NO. Dexamethasone (Dex) was used as the positive control.

opennotspecifiedDec 2021View details →

Fig. 8. Forrestline F suppressed LPSinduced inflammatory activity through Nrf2 in Diterpenoid alkaloids from Delphinium forrestii var. viride and their anti-inflammation activity

Fig. 8. Forrestline F suppressed LPSinduced inflammatory activity through Nrf2 pathways and promoted the LPSinduced nuclear translocation of Nrf2. (A–B) The cells were exposed to forrestline F (5, 10, 20 μM) for 3 h and cultured with LPS for 24 h, except for the control group. The protein expression of Keap-1/Nrf2 pathway was determined by western blotting and the quantifications of protein expression were performed by Image J. (C) The inhibitory effect of forrestline F (20 μM) on the nuclear translocation of Nrf2 induced by LPS via confocal laser scanning microscopy, in conjunction with nuclear DAPI stain (scale bar: 50 μM). The values are presented as mean ± SD of three independent experiments, n = 3. ###p <0.001, vs. the control group; ***p <0.001, vs. LPS-stimulated group.