Find research datasets worth reusing

opennotspecifiedAug 2004View details →

Figure 2 in Mitochondrial DNA sequences of the Afro-Arabian spiny-tailed lizards (genus Uromastyx; family Agamidae): phylogenetic analyses and evolution of gene arrangements

Figure 2. Evolution of mitochondrial gene organization in Uromastyx. A, typical vertebrate organization plesiomorphic to lizards. B, typical organization for acrodont lizards including Leiolepis and likely the direct common ancestor of Leiolepis and Uromastyx. C, typical Uromastyx organization in which the putative origin of light-strand replication (black box) disappeared from the WANCY tRNA gene cluster. D, organization for U. ornata and likely for the direct common ancestor of U. ornata and U. ocellata, which has an insertion containing a stem-and-loop structure (hatched box) and the second tRNAGln gene or pseudogene (Q*). E, organization for U. ocellata in which Q* disappeared. See Figs 3 and 4 for sequences and secondary structures of the inserted region in U. ornata and U. ocellata.

opennotspecifiedAug 2004View details →

Figure 3 in Mitochondrial DNA sequences of the Afro-Arabian spiny-tailed lizards (genus Uromastyx; family Agamidae): phylogenetic analyses and evolution of gene arrangements

Figure 3. Nucleotide sequences of the inserted region between the tRNA Gln and tRNAIle genes. A, alignment between the 128 bp insertion in Uromastyx ornata and the 59 bp insertion in U. ocellata (65% identity). B, alignment between the original tRNAGln gene and its second copy within the inserted region for U. ornata (49% identity). Light-strand and heavystrand sequences are shown for A and B, respectively. Dots indicate identity with the first sequence and dashes denote a gap.

opennotspecifiedAug 2004View details →

Figure 1 in Mitochondrial DNA sequences of the Afro-Arabian spiny-tailed lizards (genus Uromastyx; family Agamidae): phylogenetic analyses and evolution of gene arrangements

Figure 1. Position of primers used for amplification and/or sequencing. See Table 1 for the primer sequences; numbers of primers correspond to those in Table 1.

Fig. 6 in Genome-wide characterization and expression profiling of GASA gene family in Triticum turgidum ssp. durum (desf.) husn. (Durum wheat) unveils its involvement in environmental stress responses

Fig. 6. Predicted cis-acting elements in the TdGASA genes promoter regions. The 2-kb sequences upstream of the 19 TdGASA genes were analyzed with the PlantCARE (http://bioinformatics.psb.ugent.be/webtools/plantcare/html) and New PLACE (https://www.dna.affrc.go.jp/PLACE/?action=new place) databases. The cis-acting elements were classified into three major classes: hormone-related cis-elements, development-related cis-elements, and stress-related cis-elements.

Fig. 8 in Genome-wide characterization and expression profiling of GASA gene family in Triticum turgidum ssp. durum (desf.) husn. (Durum wheat) unveils its involvement in environmental stress responses

Fig. 8. Expression of TdGASA1, TdGASA4, TdGASA14, and TdGASA19 genes confers stress tolerance to yeast cells. Wild-type transformed with empty vector (EV) or with four TdGASA genes were grown for 4 days under normal growth conditions (30 ◦ C) or under heat (37 ◦ C or 42 ◦ C), ionic (LiCl 100 mM), salt stress (NaCl 2 M), osmotic stress (Mannitol 2 M), and oxidative stress (H2O2 10 mM) in rich solid media a containing galactose as carbon source. The growth assays depicted are reflective of three independent replicates (A). (B) Cell growth (OD600) of wild-type transformed with empty vector (EV) or with four TdGASA genes under different stress conditions. Error bars represent calculated standard error of the mean (SEM) of three independent replicates. Different letters on bars represent the significant values according to Duncan's test (p <0.05).

Fig. 7 in Genome-wide characterization and expression profiling of GASA gene family in Triticum turgidum ssp. durum (desf.) husn. (Durum wheat) unveils its involvement in environmental stress responses

Fig. 7. Expression pattern of durum wheat GASA genes. (A) Heatmap of the expression pattern of TdGASA genes in roots, stems, leaves, and seeds. (B) Heatmap showing the expression pattern of TdGASA genes T. durum plants subjected to 150 mM NaCl, 15% PEG-6000, 50 μM GA3 and 50 μM ABA. The data represent means of three independent experiments. Color code is presented above the heatmap. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig. 1 in Genome-wide characterization and expression profiling of GASA gene family in Triticum turgidum ssp. durum (desf.) husn. (Durum wheat) unveils its involvement in environmental stress responses

Fig. 1. Locations of the 19 TdGASA genes on durum wheat chromosomes. The scale on the left represented the length of the chromosomes. Mb = million base pair. The pairs of duplicated genes are underlined with same color. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig. 5 in Genome-wide characterization and expression profiling of GASA gene family in Triticum turgidum ssp. durum (desf.) husn. (Durum wheat) unveils its involvement in environmental stress responses

Fig. 5. Analysis of 19 TdGASA genes structures. (A) An unrooted phylogenetic tree constructed based on TdGASA genes sequences. (B) Exon-intron structure analysis, blue boxes represent untranslated regions, yellow boxes and black lines were exon and intron positions, respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig. 2 in Genome-wide characterization and expression profiling of GASA gene family in Triticum turgidum ssp. durum (desf.) husn. (Durum wheat) unveils its involvement in environmental stress responses

Fig. 2. Predicted three-dimensional structures of TdGASA proteins. Models were generated by using Phyr2 server. The secondary structure elements: α-helices (blue), β-sheets (yellow), and coils (cyan) are indicated for the predicted 3D structures of TdGASA proteins. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig. 4 in Genome-wide characterization and expression profiling of GASA gene family in Triticum turgidum ssp. durum (desf.) husn. (Durum wheat) unveils its involvement in environmental stress responses

Fig. 4. Analysis of Triticum durum TdGASA proteins structures. (A) An unrooted phylogenetic tree generated using TdGASA protein sequences. (B) Motif identification using MEME. (C) TdGASA protein structure (D) Multiple sequence alignments of GASA domain. (E) Logo of the TdGASA conserved-domain.

Fig. 3 in Genome-wide characterization and expression profiling of GASA gene family in Triticum turgidum ssp. durum (desf.) husn. (Durum wheat) unveils its involvement in environmental stress responses

Fig. 3. Phylogenetic tree analysis of B. distachyon, O. sativa, S. bicolor, A. thaliana, T. aestivum, T. turgidum, and H. vulgare GASA proteins. Maximum likelihood method with 1000 bootstrap replicates was used to compute the distances of GASA proteins by using the MEGA11 software. The four subgroups of GASAs are presented with different colors. Black triangles denote B distachyon (Bd) proteins, yellow triangles denote the O. sativa (Os) proteins, blue triangles denote the S. bicolor (Sb), red triangles are for A. thaliana (At) proteins, black circles refer to T. turgidum (TdGASA) proteins, blue diamond's indicate T. aestivum (Ta) proteins, and yellow diamonds are for H. vulgare (Hv) proteins. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig. 9 in Comprehensive analysis of sucrolytic enzyme gene families in carnation (Dianthus caryophyllus L.)

Fig. 9. Effects of hypoxic treatment on earlier responses of gene expression. Relative transcript levels calculated by real-time RT-PCR using DcUbq3-7 as a standard were compared before (black bars) and after 3 or 12 h of incubation under normoxic (gray bars) and hypoxic (white bars) conditions. Significant differences (P <0.05) detected by Tukey's multiple comparison test are indicated by different letters above the bars.

Fig. 5 in Comprehensive analysis of sucrolytic enzyme gene families in carnation (Dianthus caryophyllus L.)

Fig. 5. Phylogenetic tree of sucrose synthase (SUS). Amino acid sequences deduced from SUS genes of Arabidopsis, rice, and carnation, distinguished by AGI ID, locus identifier, or ORF no. shown in parentheses, were aligned by ClustalW. The tree was constructed by the neighbor-joining method using MEGA7.0 software. * indicates carnation SUS described in this study.

Fig. 8 in Comprehensive analysis of sucrolytic enzyme gene families in carnation (Dianthus caryophyllus L.)

Fig. 8. Expression profiles of sucrose synthase (SUS) gene family of carnation in petals under sucrose and hypoxic treatments. Relative transcript levels calculated by real-time RT-PCR using DcUbq3-7 as a standard were compared among petals after 24 h of incubation with distilled water (plain bars) or sucrose solution (hatched bars), under normoxic (gray bars) and hypoxic (white bars) conditions. Data are expressed as mean ± SE of three separate samples. The results of two-way analysis of variance (ANOVA) are shown below each graph; NS, *, and ** indicate not significant and significant at P <0.05 and P <0.01, respectively.

Fig. 7 in Comprehensive analysis of sucrolytic enzyme gene families in carnation (Dianthus caryophyllus L.)

Fig. 7. Expression profiles of invertase (INV) gene family of carnation in petals under sucrose and hypoxic treatments. Relative transcript levels calculated by realtime RT-PCR using DcUbq3-7 as a standard were compared among petals after 24 h of incubation with distilled water (plain bars) or sucrose solution (hatched bars), under normoxic (gray bars) and hypoxic (white bars) conditions. Data are expressed as mean ± SE of three separate samples. The results of two-way analysis of variance (ANOVA) are shown below each graph; NS, *, and ** indicate not significant and significant at P <0.05 and P <0.01, respectively.

Fig. 6 in Comprehensive analysis of sucrolytic enzyme gene families in carnation (Dianthus caryophyllus L.)

Fig. 6. Expression profiles of invertase (INV) and sucrose synthase (SUS) gene families of carnation among organs. Relative transcript levels of gene families of cellwall INV (A), vacuolar INV (B), cytoplasmic INV (C), and SUS (D) calculated by real-time RT-PCR using DcUbq3-7 as a standard were compared among leaves (dotted bars) and petals (black bars). The graph for the gene with an eminent value is shown as an inset. Data are expressed as mean ± SE of three separate samples. The result of Student's t-test is shown above the bars; NS, *, and ** indicate not significant and significant at P <0.05 and P <0.01, respectively.

Fig. 2 in Comprehensive analysis of sucrolytic enzyme gene families in carnation (Dianthus caryophyllus L.)

Fig. 2. Sugar content in carnation petals. Contents of reducing sugars (A), sucrose (B), and starch (C) were compared among petals after 24 h of incubation following pretreatment with silver-thiosulfate (STS), and supplied with distilled water (plain bars) or sucrose solution (hatched bars), under normoxic (gray bars) and hypoxic (white bars) conditions. Data are expressed as mean ± SE of three separate samples. The results of two-way analysis of variance (ANOVA) are shown below each graph; NS, *, and ** indicate not significant and significant at P <0.05 and P <0.01, respectively. *** indicates significant difference at P <0.05 detected by Dunnett's test.

Fig. 1 in Comprehensive analysis of sucrolytic enzyme gene families in carnation (Dianthus caryophyllus L.)

Fig. 1. Effects of chemical treatment on flower senescence of carnation. Carnation florets pretreated with silver-thiosulfate (STS) or incubated with sucrose (Suc, not pretreated with STS) were compared to non-pretreated control florets incubated with distilled water (DW).

Fig. 3 in Comprehensive analysis of sucrolytic enzyme gene families in carnation (Dianthus caryophyllus L.)

Fig. 3. Enzymatic activities of invertase (INV) and sucrose synthase (SUS) in carnation petals. Activities of cell-wall INV (A), vacuolar INV (B), cytoplasmic INV (C), and SUS (D) were compared among petals after 24 h of incubation following pretreatment with silver-thiosulfate (STS), and supplied with distilled water (plain bars) or sucrose solution (hatched bars), under normoxic (gray bars) and hypoxic (white bars) conditions. Data are expressed as mean ± SE of three separate samples. The results of two-way analysis of variance (ANOVA) are shown below each graph; NS and * indicate not significant and significant at P <0.05, respectively.