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dryad32/100

Data from: Digital fragment analysis of short tandem repeats by high-throughput amplicon sequencing

High-throughput sequencing has been proposed as a method to genotype microsatellites and overcome the four main technical drawbacks of capillary electrophoresis: amplification artifacts, imprecise sizing, length homoplasy, and limited multiplex capability. The objective of this project was to test a high-throughput amplicon sequencing approach to fragment analysis of short tandem repeats and characterize its advantages and disadvantages against traditional capillary electrophoresis. We amplified and sequenced 12 muskrat microsatellite loci from 180 muskrat specimens and analyzed the sequencing data for precision of allele calling, propensity for amplification or sequencing artifacts, and for evidence of length homoplasy. Of the 294 total alleles, we detected by sequencing, only 164 alleles would have been detected by capillary electrophoresis as the remaining 130 alleles (44%) would have been hidden by length homoplasy. The ability to detect a greater number of unique alleles resulted in the ability to resolve greater population genetic structure. The primary advantages of fragment analysis by sequencing are the ability to precisely size fragments, resolve length homoplasy, multiplex many individuals and many loci into a single high-throughput run, and compare data across projects and across laboratories (present and future) with minimal technical calibration. A significant disadvantage of fragment analysis by sequencing is that the method is only practical and cost-effective when performed on batches of several hundred samples with multiple loci. Future work is needed to optimize throughput while minimizing costs and to update existing microsatellite allele calling and analysis programs to accommodate sequence-aware microsatellite data.

opencc-zeroDec 2015View details →
zenodo32/100

Likert-Skala for User friendly interaction in intelligent digital assistance systems.

<p>A dataset for a usability study.</p>

opencc-by-4.0Jun 2021View details →
zenodo32/100

FIGURE 3 in Substituting time-consuming pencil drawings in arthropod taxonomy using stacks of digital photographs

FIGURE 3. Creation of the drawing with the WACOM board using a crustacean appendage as an example. a, stacks of micrographs are imported in a drawing software and arranged into one picture; focus levels can be selected by clicking on the level selector (arrow); b, the outlines of the photos are made using the levels of the stacks that are in focus; c, once all details are drawn, the photos are deselected and the drawing can be used.

opennotspecifiedNov 2006View details →
zenodo32/100

FIGURE 2 in Substituting time-consuming pencil drawings in arthropod taxonomy using stacks of digital photographs

FIGURE 2. Making of micrographs. a, stacks of photos are taken with a camera mounted on a microscope (1), directly transferred onto a computer (2) and drawn with a WACOM Intuous 2 DIN A3 drawing tablet (3); b, all camera functions are controlled by the remote capture task software, in the preview window of this software where focus of the microscope is being controlled. Moving the lever 1 up will zoom out the dark margins; pushing the button 2 will expose a picture and automatically transfers it onto the computer.

opennotspecifiedNov 2006View details →
zenodo32/100

FIGURE 1 in Substituting time-consuming pencil drawings in arthropod taxonomy using stacks of digital photographs

FIGURE 1. Preparation of permanent slides. a, maceration of appendages on a hot plate removing tissue from the appendages; b, creation of permanent slides using Euparal, arrow points to a cover slip with drop of Euparal, resting to allow the mounting medium to becoming somewhat harder; c, lignin-pink stained distal part of a leg of an amphipod crustacean mounted in Euparal.

opennotspecifiedNov 2006View details →
zenodo32/100

Digital breast tomosynthesis and contrast-enhanced dual-energy digital mammography alone and in combination compared to 2D digital synthetized mammography and MR imaging in breast cancer detection and classification

<p>We uploaded the dataset of included patients of manuscript:&nbsp;Petrillo A, Fusco R, Vallone P, Filice S, Granata V, Petrosino T, Rosaria Rubulotta M, Setola SV, Mattace Raso M, Maio F, Raiano C, Siani C, Di Bonito M, Botti G. Digital breast tomosynthesis and contrast-enhanced dual-energy digital mammography alone and in combination compared to 2D digital synthetized mammography and MR imaging in breast cancer detection and classification. Breast J. 2020 May;26(5):860-872. doi: 10.1111/tbj.13739. Epub 2019 Dec 30. PMID: 31886607.</p>

opencc-by-4.0May 2020View details →
zenodo32/100

Evaluation of average glandular dose and investigation of the relationship with compressed breast thickness in dual energy contrast enhanced digital mammography and digital breast tomosynthesis.

<p>We uploaded the&nbsp;average absorbed glandular dose (AGD), compressed breast thickness (CBT) and compression force (CF) values&nbsp;of of Dual energy contrast enhanced digital mammography (CEDM)&nbsp;related to the manuscript: Fusco R, Raiano N, Raiano C, Maio F, Vallone P, Mattace Raso M, Setola SV, Granata V, Rubulotta MR, Barretta ML, Petrosino T, Petrillo A. Evaluation of average glandular dose and investigation of the relationship with compressed breast thickness in dual energy contrast enhanced digital mammography and digital breast tomosynthesis. Eur J Radiol. 2020 May;126:108912. doi: 10.1016/j.ejrad.2020.108912.&nbsp;</p>

opencc-by-4.0Mar 2020View details →
zenodo32/100

Usibility Test for User friendly interaction in intelligent digital assistance systems.

<p>Te Interview for usability test.</p>

opencc-by-4.0Jun 2021View details →
zenodo32/100

Figure 4. Above, digital X in Six species of Amazonian Woodcreepers (Aves: Dendrocolaptidae) preying upon lizards and frogs

Figure 4. Above, digital X-ray of Gonatodes humeralis (USNM 568677) indicating the bones from the gecko skeleton that are more resistant to digestion. Below, histogram indicating the total number and percentage of bones recovered from at least eight geckos preyed upon by six birds. Scale bar 10 mm.

opennotspecifiedDec 2012View details →
zenodo32/100

Data for: Using a digital twin of an electrical stimulation device to monitor and control the electrical stimulation of cells in vitro

<p>Replication data for &quot;Using a digital twin of an electrical stimulation device to monitor and control the electrical stimulation of cells in vitro&quot;.</p>

opencc-by-nc-sa-4.0Aug 2021View details →
zenodo32/100

Fig. 4. A in Identification of Sound-Producing Hydrophilid Beetles (Coleoptera: Hydrophilidae) in Underwater Recordings Using Digital Signal Processing

Fig. 4. A half-second vocalization by Tropisternus blatchleyi from 0–5,000 Hz changed into the frequency domain using the Fast Fourier Transformation. Active call frequency regions are at 1,100 Hz and 4,400 Hz. The first feature for T. blatchleyi divides the sum of the points in the active frequency band by the sum of the points in the inactive band, yielding a large number in T. blatchleyi exemplar calls.

opennotspecifiedJun 2015View details →
zenodo32/100

Fig. 3 in Identification of Sound-Producing Hydrophilid Beetles (Coleoptera: Hydrophilidae) in Underwater Recordings Using Digital Signal Processing

Fig. 3. The classifier algorithm with two features shown in a two-dimensional graph. Beetle call data and noise data are classified based on two beetle call features: difference in active frequency range shape (x-axis and in Matlab™ as a template) and a ratio of amplitudes in an active and non-active frequency range (y-axis). The algorithm is shown as a solid black line. Most beetle calls fall within the correct classification in the lower left-hand corner, however, some fall outside the equation and are classified as noise. Likewise, noises are occasionally classified as beetles. As more features are added, the algorithm becomes multidimensional.

opennotspecifiedJun 2015View details →
zenodo32/100

Fig. 2 in Identification of Sound-Producing Hydrophilid Beetles (Coleoptera: Hydrophilidae) in Underwater Recordings Using Digital Signal Processing

Fig. 2. Five distress calls by Berosus pantherinus transformed into the frequency domain using the fast fourier transformation from 0–12,000 Hz (x-axis). Wide active frequency bands can be seen from 1,500–6,000 Hz and 7,000–9,500 Hz. The feature for distress calls is the sum of the data points between 1,000–6,000 Hz with an amplitude (y-axis) greater than 2.

opennotspecifiedJun 2015View details →
zenodo32/100

supplement videos of Solution for Mass Production of High-Throughput Digital Microfluidic Chip Based on Thin Film Transistor Technology

<p>supplement videos of Solution for Mass Production of High-Throughput Digital Microfluidic Chip Based on Thin Film Transistor Technology</p>

opencc-by-4.0Aug 2021View details →
zenodo32/100

RAGAM BAHAN AJAR BIPA PADA MASA PANDEMI COVID-19: SEBUAH TUNTUTAN LITERASI DIGITAL

<p>Menciptakan pembelajaran yang menyenangkan pada masa pandemic Covid-19 menjadi salah satu tantangan saat proses pembelajaran, khususnya pada pemelajar BIPA. Pemilihan ragam bahan yang tepat dapat menentukan motivasi dan kualitas hasil belajar dari pemelajar. Bahan ajar buku teks BIPA yang sudah ada perlu variasi saat penggunaanya dalam proses pembelajaran sesuai dengan tuntuang situasi dan kondisi, khusunya secara daring. Ragam bahan ajar yang diperlukan sesuai situasi Pandemi Covid-19 adalah ragam bahan ajar berbasis digital. Tuntutan menciptakan pembelajaran yang menyenangkan berkolerasi dengan kemampuan literasi digital yang dimiliki oleh pengajar. Plat form pembelajaran daring menjadi salah satu alteranatif yang dapat digunakan, namun hal tersebut tidaklah cukup untuk mengajarkan keterampilan berbahasa. Tulisan ini merupakan pengalaman penulis sebagai pengajar BIPA di Vietnam secara daring saat masa Pandemi Covid-19.</p>

opencc-by-4.0Aug 2021View details →
dryad32/100

Digital holographic microscope recordings of passive motion of small particles

<p>Digital holographic microscopy provides the ability to observe throughout a volume that is large compared to its resolution without the need to actively refocus to capture the entire volume. This enables simultaneous observations of large numbers of small objects within such a volume. We have constructed a microscope that can observe a volume of 0.4 x 0.4 x 1.0 µm with submicrometer resolution (in xy) and 2 µm resolution (in z) for observation of microorganisms and minerals in liquid environments on Earth and on potential planetary missions. Because environmental samples are likely to contain mixtures of inorganics and microorganisms of comparable sizes near the resolution limit of the instrument, discrimination between living and non-living objects may be difficult. The active motion of motile organisms can be used to readily distinguish them from non-motile objects (live or inorganic), but additional methods are required to distinguish non-motile organisms and inorganic objects that are of comparable size but different composition and structure. We demonstrate the use of passive motion to make this discrimination by evaluating diffusion and buoyancy characteristics of cells, styrene beads, alumina particles, and gas-filled vesicles of micron scale in the field of view.</p>

opencc-zeroSep 2021View details →
zenodo32/100

FIGURE 1 in Toward a biogeographic regionalization of the Nearctic region: Area nomenclature and digital map

FIGURE 1. Map of the regionalization of the Nearctic region, including three subregions, one transition zone and 29 provinces.

opennotspecifiedAug 2021View details →
zenodo32/100

Dataset for Robot-mounted digital 3D-microscope versus standard microscope for spinal decompression surgery

<p>Compilation of all data collected in the study.</p> <p>-&nbsp;Depth Perception Robotc: Data collected during depth perception experiments with the BHS RoboticScope</p> <p>-&nbsp;Depth Perception Cons: Data collected during depth perception experiments with the OM</p> <p>-&nbsp;Both Scopes Combined: Combined data regarding depth perception experiments</p> <p>-&nbsp;Questionaire &nbsp;Combined: Combined data regarding cadaveric experiments</p> <p>-&nbsp;Questionaire expsurg1:&nbsp;completed questionnaire by the experienced surgeon 1</p> <p>-&nbsp;Questionaire expsurg3:&nbsp;completed questionnaire by the experienced surgeon 3</p>

opencc-by-2.0Sep 2021View details →
zenodo32/100

Dataset for effects of shear zone thickness in root-reinforced soils: Fallow P X-ray CT scans and digital volume correlation data

<p>Dataset for effects of shear zone thickness in root-reinforced soils: Fallow P X-ray CT scans and digital volume correlation data</p>

opencc-by-4.0Nov 2019View details →
zenodo32/100

Dataset for effects of shear zone thickness in root-reinforced soils: Gorse A X-ray CT scans and digital volume correlation data

<p>Dataset for effects of shear zone thickness in root-reinforced soils: Gorse A X-ray CT scans and digital volume correlation data</p>

opencc-by-4.0Nov 2019View details →

ScienceDex guides

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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.

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Allen Brain Atlas

Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.

allen-brain-atlas
neuroscienceopenDocumentation, web resources, and API references are available online.
Last verified 2026-04-30Open record

Annotated Behaviour and Observability Dataset (ABODe)

ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.

abode-home-cage
behavioral-neuroscienceopenThe DataShare record exposes download links for annotations, documentation, license text, and the zipped per-snippet data directory.
Last verified 2026-04-30Open record

DANDI Archive for NWB datasets

DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.

dandi-nwb
electrophysiologyopenPublished Dandiset metadata and archive endpoints are available through the production DANDI API.
Last verified 2026-04-30Open record

International Brain Laboratory public data

The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.

ibl
behavioral-neuroscienceopenPublic sessions can be searched and loaded from the IBL public data server through ONE.
Last verified 2026-04-29Open record

OpenNeuro

OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.

openneuro
neuroscienceopenPublished datasets are available on demand over the internet.
Last verified 2026-04-29Open record

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