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opennotspecifiedJan 2022View details →

Fig. 3 in Rapid screening of glycosyltransferases in plants using a linear DNA expression template based cell-free transcription-translation system

Fig. 3. UPLC-MS analysis of isoquercitrin converted from quercetin by ArUGTs expressed in TX-TL. (A) A glycosylation reaction catalyzed by a UDP-glucose glycosyltransferase. (B) The chromatogram of the blank sample is 50% methanol solvent. (C) The chromatogram of the negative group, which has TXTL, quercetin, UDP-glucose but no additional DNA. (D) The chromatogram of quercetin standard. (E) The chromatogram of isoquercitrin standard. (F–K) Chromatograms of products from the catalysis of quercetin by different ArUGTs (the final concentrations of the linear DNAs used for each ArUGTs are 30 nM): (F) AR14572, (G) AR11662, (H) AR43718, (I) AR06047, (J) AR06981, and (K) AR07558, see Supplementary Fig. S5 for the duplicate group. The y axis indicates ion signal abundance relative to the highest signal in each chromatogram (%). The numbers in the top right of each chromatogram (for instance 2.21e7 in [E]) indicate the ion counts represented by "100" relative abundance. The retention time of the isoquercitrin standard is 4.08 min, and the red arrow in each figure indicates the retention time of the product isoquercitrin. The numbers under the protein names are the peak intensities of the product. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 8 in Rosmarinic acid and hesperidin regulate gas exchange, chlorophyll fluorescence, antioxidant system and the fatty acid biosynthesis-related gene expression in Arabidopsis thaliana under heat stress

Fig. 8. The changes of the relative expression of WRI1 (A), BCCP2 (B), FAD2 (C), FAD3 (D) genes encoding after rosmarinic acid (RA, 100 μM) and/or hesperidin (HP, 100 μM) with/without heat stress (38 ◦C) in A. thaliana leaves. Multivariate data analyses of gene expression analysis under investigation.

Fig. 5 in Rosmarinic acid and hesperidin regulate gas exchange, chlorophyll fluorescence, antioxidant system and the fatty acid biosynthesis-related gene expression in Arabidopsis thaliana under heat stress

Fig. 5. The changes of the relative band intensity of different types of glutathione S-transferase activity isoenzymes (GST, A) and GST activity (B), and glutathione peroxidase activity (GPX, C) after rosmarinic acid (RA, 100 μM) and/or hesperidin (HP, 100 μM) with/without heat stress (38 ◦C) in A. thaliana leaves.

Fig. 1 in Rosmarinic acid and hesperidin regulate gas exchange, chlorophyll fluorescence, antioxidant system and the fatty acid biosynthesis-related gene expression in Arabidopsis thaliana under heat stress

Fig. 1. The average absorption per active reaction center (ABS/RC), the electron transport flux per active reaction center (ETo/RC), the flux of trapped exciton per active reaction center (TRo/RC), and the energy flow on the acceptor side of PSI ((Ro), The number of QA-reducing reaction centers per PSII antenna chlorophyll ((ΦPo/(1-ΦPo)), the efficiency with which a trapped exciton transfers an electron to the photosynthetic electron transfer chain ((ΨEo/(1-ΨEo)), the number of QA- reducing reaction centers per PSII antenna chlorophyll (γRC/(1-γRC)), the ratio of total dissipation to the number of active reaction centers (DIo/RC). The relative variable fluorescence intensity at J (VJ) and I step (VI), the performance index based on light absorption (PIABS), and the performance index (potential) for energy conservation from exciton to PSI and acceptor reduction (PItotal) were determined in A. thaliana leaves following treatment with rosmarinic acid (RA, 100 μM) and/or hesperidin (HP, 100 μM) with/without heat stress (38 ◦ C).

Fig. 7 in Rosmarinic acid and hesperidin regulate gas exchange, chlorophyll fluorescence, antioxidant system and the fatty acid biosynthesis-related gene expression in Arabidopsis thaliana under heat stress

Fig. 7. The changes of monodehydroascorbate reductase activity (MDHAR, A), dehydroascorbate reductase activity (DHAR, B), ascorbate content (AsA, C), dehydroascorbate content (DHA, D), glutathione content (GSH, E), oxidized glutathione content (GSSG, F), AsA/DHA (G), GSH/GSSG (H) and GSH redox state (I) after rosmarinic acid (RA, 100 μM) and/or hesperidin (HP, 100 μM) with/without heat stress (38 ◦C) in A. thaliana leaves.

Fig. 4 in Rosmarinic acid and hesperidin regulate gas exchange, chlorophyll fluorescence, antioxidant system and the fatty acid biosynthesis-related gene expression in Arabidopsis thaliana under heat stress

Fig. 4. The changes of the relative band intensity of different types of peroxidase isoenzymes (POX, A) and POX activity (B), relative band intensity of different types of NADPH oxidase isoenzymes (NOX, C) and NOX activity (D) after rosmarinic acid (RA, 100 μM) and/or hesperidin (HP, 100 μM) with/without heat stress (38 ◦C) in A. thaliana leaves.

Fig. 3 in Rosmarinic acid and hesperidin regulate gas exchange, chlorophyll fluorescence, antioxidant system and the fatty acid biosynthesis-related gene expression in Arabidopsis thaliana under heat stress

Fig. 3. The changes of the relative band intensity of different types of superoxide dismutase isoenzymes (SOD, A) and SOD activity (B), the relative band intensity of different types of catalase isoenzymes (CAT, C) and CAT activity (D) after rosmarinic acid (RA, 100 μM) and/or hesperidin (HP, 100 μM) with/without heat stress (38 ◦C) in A. thaliana leaves.

Fig. 2 in Rosmarinic acid and hesperidin regulate gas exchange, chlorophyll fluorescence, antioxidant system and the fatty acid biosynthesis-related gene expression in Arabidopsis thaliana under heat stress

Fig. 2. The changes of hydrogen peroxide content (H O, A), lipid peroxidation (TBARS content, B), histochemical staining for O • accumulation (C), histochemical 2 2 2 staining for H2O2 determination (D), histochemical staining for plasma membrane integrity (E) and histochemical staining for lipid peroxidation (F) after rosmarinic acid (RA, 100 μM) and/or hesperidin (HP, 100 μM) with/without heat stress (38 ◦C) in A. thaliana leaves.

Fig. 6 in Rosmarinic acid and hesperidin regulate gas exchange, chlorophyll fluorescence, antioxidant system and the fatty acid biosynthesis-related gene expression in Arabidopsis thaliana under heat stress

Fig. 6. The changes of the relative band intensity of different types of ascorbate glutathione isoenzymes (APX, A) and APX activity (B), and glutathione reductase activity (GR, C) after rosmarinic acid (RA, 100 μM) and/or hesperidin (HP, 100 μM) with/without heat stress (38 ◦C) in A. thaliana leaves.

opencc-by-4.0Aug 2023View details →

Dataset of scientific article: "Effect of miR-34a on expression of clock and clock-controlled genes in human cancer DLD1 and Lovo cells with different backgrounds in respect to p53 functionality and 17β-estradiol-mediated regulation"

<p>The dataset includes "raw" data used for the calculation of gene expression, wound healing test, fluorescence intensity, and MTS test.</p> <p> </p>

Fig. 7 in Triterpenic and phenolic acids production changed in Salvia officinalis via in vitro and in vivo polyploidization: A consequence of altered genes expression

Fig. 7. Relative expression of six genes related to the biosynthesis of TAs, including farnesyl diphosphate synthase (FDS), squalene synthase (SQS), squalene epoxidases (SQE), lupeol synthase (LUS), β-amyrin synthase (BAS), and mixed function amyrin synthase (MFAS) genes in the different ploidy levels of in vitro and in vivo conditions of S. officinalis. Error bars are shown as standard deviation (n = 3).

Fig. 6 in Triterpenic and phenolic acids production changed in Salvia officinalis via in vitro and in vivo polyploidization: A consequence of altered genes expression

Fig. 6. The observation of stomata characteristics in vivo plants of diploid (A1,2) and mixoploid (B1,2) of S. officinalis. Bars = 50 and 10 μm.

Fig. 5 in Triterpenic and phenolic acids production changed in Salvia officinalis via in vitro and in vivo polyploidization: A consequence of altered genes expression

Fig. 5. The observation of stomata characteristics in vitro plants of diploid (A1,2), tetraploid (B1,2), and mixoploid (C1,2) of S. officinalis. Bars = 50 and 10 μm.

Fig. 2 in Triterpenic and phenolic acids production changed in Salvia officinalis via in vitro and in vivo polyploidization: A consequence of altered genes expression

Fig. 2. Chromosome numbers of root tip cells from diploid plants 2n = 2x = 14 (A); and tetraploid plants 2n = 4x = 28 (B) of S. officinalis.

Fig. 1 in Triterpenic and phenolic acids production changed in Salvia officinalis via in vitro and in vivo polyploidization: A consequence of altered genes expression

Fig. 1. Histograms of flow cytometric analysis of diploid (A), tetraploid (B) and mixoploid (C) plants of S. officinalis.

Fig. 4 in Triterpenic and phenolic acids production changed in Salvia officinalis via in vitro and in vivo polyploidization: A consequence of altered genes expression

Fig. 4. The morphological characteristics of in vivo plants of diploid (A), and mixoploid (B) of S. officinalis.

Fig. 8 in Triterpenic and phenolic acids production changed in Salvia officinalis via in vitro and in vivo polyploidization: A consequence of altered genes expression

Fig. 8. Pearson correlation and the relationship between TAs contents of betulinic acid (BA), ursolic acid (UA), and oleanolic acid (OA) and gene expression related to triterpenic acids biosynthesis pathway including farnesyl diphosphate synthase (FDS), squalene synthase (SQS), squalene epoxidases (SQE), lupeol synthase (LUS), β-amyrin synthase (BAS), and mixed function amyrin synthase (MFAS) genes in different ploidy levels of in vitro and in vivo conditions of S. officinalis.

Fig. 8 in Development of an efficient transient expression system for Siraitia grosvenorii fruit and functional characterization of two NADPH-cytochrome P450 reductases

Fig. 8. HPLC chromatogram of the mogrol standard and Agrobacterium-mediated transient expression of fruit harbouring pTRV-SgCPRs and pTRV-0 at 24 h after agroinfiltration.

Fig. 9 in Development of an efficient transient expression system for Siraitia grosvenorii fruit and functional characterization of two NADPH-cytochrome P450 reductases

Fig. 9. (A) Mogrol content in the S. grosvenorii fruit harbouring PBI121, PBI121-SgCPR1, and PBI121-SgCPR2. (B) MIIE content in the S. grosvenorii fruit harbouring PBI121, PBI121-SgCPR1, and PBI121-SgCPR2. (C) MIII content in the S. grosvenorii fruit harbouring PBI121, PBI121-SgCPR1, and PBI121-SgCPR2. (D) Accumulation of mogrol in the S. grosvenorii fruit harbouring pTRV, pTRV-SgCPR1, and pTRV-SgCPR2. (E) Accumulation of MII in the S. grosvenorii fruit harbouring pTRV, pTRV- SgCPR1, and pTRV-SgCPR2. (F) Accumulation of MIII in the S. grosvenorii fruit harbouring pTRV, pTRV-SgCPR1, and pTRV-SgCPR2. The values are the mean ± SD of three independent biological replicates.

Fig. 7 in Development of an efficient transient expression system for Siraitia grosvenorii fruit and functional characterization of two NADPH-cytochrome P450 reductases

Fig. 7. HPLC chromatogram of the mogrol standard and Agrobacterium-mediated transient expression of fruit harbouring PBI121-SgCPRs and PBI121 at 24 h after agroinfiltration.