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325 results for “PURIFICATION”
Clinical Study to Evaluate the Efficacy of Blood Purification Therapy on Cholesterol Crystal Embolism
ClinicalTrials.gov study NCT01726868. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Purification of the N-HEAT_81-1643_Additional Heparin step
<p>Previously, I purified the HTT N-HEAT_81-1643 domain and showed that the HTT N-HEAT_81-1643 domain also elutes early from a Superose 6 column 10/300 (<a href="https://zenodo.org/record/3462496#.XY4limYpA2x">https://zenodo.org/record/3462496#.XY4limYpA2x</a>). One hypothesis we proposed that could explain the early elution volume is that the construct HTT N-HEAT_81-1643 forms a complex with nucleic acid material. Here we added an additional purification step with heparin resin to remove nucleic acid material from the sample. This strategy was successful for full length HTT+HAP40 samples in removing nucleic acid and other impurities from the samples (<a href="https://zenodo.org/record/2628064#.XYTL7WYpDcs">https://zenodo.org/record/2628064#.XYTL7WYpDcs</a>) (performed by Dr. Rachel Harding).</p> <p>Purified HTT N-HEAT_81-1643 domain sample was used to perform a buffer screen and biophysical characterization with <a href="https://zenodo.org/record/3519364#.Xefhf9VOnb0">DSLS</a>, <a href="https://zenodo.org/record/3561087#.Xefi1NVOnb0">DSF</a> and <a href="https://zenodo.org/record/3561096#.Xefiu9VOnb0">DLS</a>.</p>
Purification of the HTT N-HEAT (81-1643) with various buffer conditions
<p>Previously, I purified the HTT N-HEAT_81-1643 domain and showed that the HTT N-HEAT_81-1643 domain elutes early from a Superose 6 column 10/300 (<a href="https://zenodo.org/record/3462496#.XjsVXSNOk2w">https://zenodo.org/record/3462496#.XjsVXSNOk2w</a>). Initial biophysical characterization by DLS showed samples of the HTT N-HEAT_81-1643 construct are made up of large particles ( <a href="https://zenodo.org/record/3562523#.Xjw57iN">https://zenodo.org/record/3562523#.Xjw57iN</a>, <a href="https://zenodo.org/record/3561096#.Xjw6ByNOk2w">https://zenodo.org/record/3561096#.Xjw6ByNOk2w</a>). Thus, our initial results all pointed out at having high oligomeric states of the HTT N-HEAT_81-1643 construct in solution.</p> <p> </p> <p>To rule out the possibility of a weak interaction with nucleic acid material which could cause the large particle size in solution, we tested whether we could remove the nucleic acid material by adding an additional purification step with heparin resin. However, purification using the additional heparin step showed no improvement in the purity of the sample from other protein impurities or nucleic acid material (<a href="https://zenodo.org/record/3562523#.XjsVyyNOk2w">https://zenodo.org/record/3562523#.XjsVyyNOk2w</a>).</p> <p> </p> <p>Determination of ideal buffer conditions for the HTT N-HEAT_81-1643 construct using DSLS and DSF (<a href="https://zenodo.org/record/3562523#.Xjw57iN">https://zenodo.org/record/3562523#.Xjw57iN</a></p> <p><a href="https://zenodo.org/record/3519364#.Xjw6ICNOk2w">https://zenodo.org/record/3519364#.Xjw6ICNOk2w</a>, <a href="https://zenodo.org/record/3561087#.Xjw6LiNOk2w">https://zenodo.org/record/3561087#.Xjw6LiNOk2w</a>) was not possible as no significant changes in the T<sub>aag</sub> or T<sub>m</sub> were observed for the conditions tested. Further, the data for both DSF and DSLS could have presented challenges to fit (<em>e.g.</em> The DSF data showed very high initial fluorescence while the DSLS data showed the T<sub>agg </sub>­ for this construct was at the end of the detection limit ~ 90 °C).</p> <p> </p> <p>Thus, to further assess if we could find an ideal buffer condition where the HTT N-HEAT_81-1643 construct is the most stable and monomeric, we tested different purification conditions. </p> <p> </p>
PURIFICATION OF NATURAL GAS FROM CO2 BY ADSORPTION METHOD
Open the record for dataset details and reuse information.
Large scale expression and purification of full length huntingtin Q23 from baculovirus expression system (2016/08/29)
<p>Open lab notebook huntingtin structure function project.<br> </p>
Expression and purification of huntingtin domain constructs spanning aa. P80-G428 (2016/11/29)
<p>Open lab notebook huntingtin structure function project.<br> </p>
The artificial-liver blood-purification system can effectively improve hypercytokinemia for COVID-19
<p><span><span>Since December 2019, the outbreak of Coronary Virus Disease 2019 (COVID-19) occurred in Wuhan, and rapidly spread across the country, and the world. As the number of diagnoses and deaths continues to rise, this has become the focus of international public health. COVID-19 is highly contagious, and there is no effective treatment. New treatment strategies are urgently needed to improve the treatment success rate of severe and critically ill patients. Increasing evidence has shown that cytokine storm plays an important role in the progression of COVID-19. The artificial liver blood purification system (ALS) is expected to improve the cytokine storm. In the present study, the levels of cytokines and chemokines were detected in 12 COVID-19 patients before and after ALS, and exciting results were obtained. The present study has directly proven that ALS can block the cytokine storm, rapidly remove the inflammatory mediators, and hopefully suppress the progression of the disease, providing a new idea for the clinical treatment of COVID-19.</span></span></p>
Identification of B6T173 (ZmPrx35) as the prevailing peroxidase in highly insect-resistant maize (Zea mays, p84C3) kernels by activity-directed purification
<p>Dataset for publication https://doi.org/10.3389/fpls.2015.00670</p>
Expression and purification of PARP1 from Sf9 Insect cells 2019/02/04
<p><strong>Project: </strong>Investigating HTT parylation and PAR-binding in its putative role in DNA damage repair</p> <p><strong>Experiment: </strong>Expression and purification of PARP1 from Sf9 Insect cells</p> <p><strong>Date completed:­ </strong>2019/02/04</p> <p><strong>Rationale: </strong>HTT may be parylated or recognize PAR motifs on other proteins involved in DNA damage repair. To test this hypothesis, the full-length PARP1 cDNA was cloned in to pFBOH-SBP-TEV-LIC, overexpressed in insect cell Sf9 culture and purified. The purified PARP1 protein will be shared with Dr. Tamara Maiuri for her future experiments on the link between HTT protein and parylation. </p>
Large-scale purification of Q23 and Q54 HTT-HAP40 from Sf9 expression system 2019/07/29
<p><strong>Project: </strong>Biophysical investigation of purified HTT protein samples</p> <p><strong>Experiment: </strong>Large-scale purification of Q23 and Q54 HTT-HAP40 from Sf9 expression system</p> <p><strong>Date completed:­ </strong>2019/07/29</p> <p><strong>Rationale: </strong>HTT samples are to be purified from different systems for subsequent biophysical and structural studies. </p>
Large-scale purification of Q23 and Q54 HTT from Sf9 and EXPI293F expression systems 2019/07/22
<p><strong>Project: </strong>Biophysical investigation of purified HTT protein samples</p> <p><strong>Experiment: </strong>Large-scale purification of Q23 and Q54 HTT from Sf9 and EXPI293F expression systems</p> <p><strong>Date completed:­ </strong>2019/07/22</p> <p><strong>Rationale: </strong>HTT samples are to be purified from different systems for subsequent biophysical and structural studies. </p>
Purification of HTT aa. 76-3060 -HAP40 aa. 1-371 for structural analysis 2019/10/21
<p><strong>Project: </strong>Biophysical investigation of purified HTT protein samples</p> <p><strong>Experiment: </strong>Purification of HTT<sup>76-3060 </sup>-HAP40<sup>1-371</sup> from Sf9 </p> <p><strong>Date completed:­ </strong>2019/10/21</p> <p><strong>Rationale: </strong>Purification of HTT<sup>76-3060 </sup>-HAP40<sup>1-371</sup> for structural analysis</p>
Large scale expression and purification of full-length huntingtin Q54 with HAP40 from baculoviral expression system production in sf9 insect cells – 2018/08/04
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Rationale </strong>- Purified huntingtin samples with and without stabilising binding partners (HAP40) are required for use in structural and functional studies, in particular SAXS experiments. </p>
Supplementary material 2 from: Horváthová E (2022) Analysis of Drinking Water treatment costs – with an Application to Groundwater Purification Valuation. One Ecosystem 7: e82125. https://doi.org/10.3897/oneeco.7.e82125
Ecosystem types
Supplementary material 1 from: Horváthová E (2022) Analysis of Drinking Water treatment costs – with an Application to Groundwater Purification Valuation. One Ecosystem 7: e82125. https://doi.org/10.3897/oneeco.7.e82125
Regression results
Procyanidins from cranberry press residues - extraction opti-mization, purification and characterization HRMS data
<p>Data of HRMS</p>
Fig. 6 in Isolation, purification, and characterization of pectin methylesterase inhibitor and polygalacturonase inhibitor protein from Indian lemon (Citrus limon L.)
Fig. 6. Circular dichroism spectra of (a) purified PMEI and (b) purified PGIP.
Fig. 2 in Isolation, purification, and characterization of pectin methylesterase inhibitor and polygalacturonase inhibitor protein from Indian lemon (Citrus limon L.)
Fig. 2. Denaturing SDS-PAGE (12% gel) of purified PMEI and PGIP stained with silver staining.
Extraction, purification, and clumped isotope analysis of methane (Δ13CDH3 and Δ12CD2H2) from different sources and the atmosphere
<p>This is the dataset needed to replicate the figures in the paper submitted to AMT.</p>
Changes in Microvascular Perfusion During Blood Purification With Cytosorb® in Septic Shock
ClinicalTrials.gov study NCT03456180. IPD Sharing: Not stated. Countries: 0. Publications: 15.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.