Expression and purification of PARP1 from Sf9 Insect cells 2019/02/04

<p><strong>Project:&nbsp;</strong>Investigating HTT parylation and PAR-binding in its putative role in DNA damage repair</p> <p><strong>Experiment:&nbsp;</strong>Expression and purification of PARP1 from Sf9 Insect cells</p> <p><strong>Date completed:&shy;&nbsp;</strong>2019/02/04</p> <p><strong>Rationale:&nbsp;</strong>HTT may be parylated or recognize PAR motifs on other proteins involved in DNA damage repair. To test this hypothesis, the full-length PARP1 cDNA was cloned in to pFBOH-SBP-TEV-LIC, overexpressed in insect cell Sf9 culture and purified. The purified PARP1 protein will be shared with Dr. Tamara Maiuri for her future experiments on the link between HTT protein and parylation.&nbsp;</p>